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Appendage Development Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Appendage Development Pathway and Ectodermal Dysplasia, Dysplasia, Nervousness, Tissue Adhesions, Inflammation. The study of the Appendage Development Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Appendage Development Pathway has been researched in relation to Segmentation, Neurogenesis, Rna Interference, Limb Development, Hair Follicle Development. The Appendage Development Pathway complements our catalog of research reagents including antibodies and ELISA kits against WNT, TNF, SHH, DSPP, EDA.

Top Research Reagents

We have 3238 products for the study of the Appendage Development Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

UVRAG Antibody
NBP1-18885
Western Blot: UVRAG Antibody [NBP1-18885] - Beclin 1 is acetylated at lysines 430 and 437. TSA and NAM increase the binding of Beclin 1 to Rubicon. Immunoprecipitation of indicated Beclin 1-binding partners with ectopically expressed Flag-Beclin 1 in HEK293T cells treated with TSA and NAM. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms8215), licensed under a CC-BY license.Immunohistochemistry-Paraffin: UVRAG Antibody [NBP1-18885] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti- UVRAG used at a dilution of 1:200 (1ug/ml). Detection: DAB

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

3 Publications
NFkB p105/p50 Antibody (2J10D7) - BSA Free
NB100-56583
Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review

Mouse Monoclonal
Species Human, Rat, Rabbit
Applications WB, Flow, IHC

     1 Review

5 Publications
LIM1 Antibody (OTI2D5)
NBP2-01926
Western Blot: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM1.Immunocytochemistry/Immunofluorescence: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM1.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

4 Publications
TROY/TNFRSF19 Antibody [Unconjugated]
AF723

Goat Polyclonal
Species Mouse
Applications WB, IHC, ELISA(Cap)

1 Publication
EDAR Antibody [Unconjugated]
AF745

Goat Polyclonal
Species Mouse
Applications WB, Block

6 Publications
Desmocollin-3 Antibody (765129)
MAB7265
Western blot shows lysates of mouse skin tissue and mouse embryo (15 d.p.c.) tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Desmocollin-3 Monoclonal Antibody (Catalog # MAB7265) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=Desmocollin-3 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse Desmocollin-3 Monoclonal Antibody (Catalog # MAB7265) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Rat Monoclonal
Species Mouse
Applications WB, IHC

     1 Review

RFC1 Antibody [Unconjugated]
AF6457
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human RFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6457) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

Sheep Polyclonal
Species Human
Applications WB

CKAP4/p63 Antibody [Unconjugated]
AF7355
Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line, TK-6 human lymphoblast cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CKAP4/p63 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7355) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (<a class=CKAP4/p63 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human CKAP4/p63 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7355) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; <a class=

Sheep Polyclonal
Species Human
Applications WB, Simple Western, ICC

2 Publications
p63/TP73L Antibody [Unconjugated]
AF1916
p63/TP73L was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=p63/TP73L was detected in immersion fixed SCC-25 human tongue carcinoma cell line using Goat Anti-Human p63/TP73L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1916) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, IHC, ICC

     1 Review

23 Publications
TGF-beta 1 [Unconjugated]
7754-BH/CF
Recombinant human TGF-beta 1 (<a class=NoLineLink href=


Species Human
Applications BA

53 Publications
EDA-A1/Ectodysplasin A1
3944-ED


Species Human
Applications Bind

3 Publications
Sonic Hedgehog/Shh [Unconjugated]
464-SH
Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus (Catalog # 464-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The activity is more than 30-fold greater than the top competitor's Sonic Hedgehog.Recombinant Mouse Sonic Hedgehog/Shh (C25II), N-Terminus (Catalog # 464-SH) has a molecular weight (MW) of 20.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).


Species Mouse
Applications BA

65 Publications
TNF-alpha [Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.


Species Human
Applications BA

     3 Reviews

808 Publications
EGF [Unconjugated]
236-EG
Recombinant Human EGF Protein (Catalog # 236-EG) has a molecular weight (MW) of 6.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED<sub>50</sub> for this effect is 20‑100 pg/mL.


Species Human
Applications BA

793 Publications
RFC2 Antibody (OTI2B2) - Azide and BSA Free
NBP2-73857
Western Blot: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC2.Immunohistochemistry: RFC2 Antibody (OTI2B2) - Azide and BSA Free [NBP2-73857] - Analysis of Human prostate tissue.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, IHC

RPE65 Antibody (401.8B11.3D9) - BSA Free
NB100-355
Western Blot: RPE65 Antibody (401.8B11.3D9) - BSA Free [NB100-355] - Subcellular localization of BEST1 and surface Ca2+-dependent Cl- current in patient-derived iPSC-RPEs. Western blots show similar BEST1 expression levels in WT and patient-derived iPSC-RPEs. Each sample was from one cell lysis (BEST1 and beta-actin, RPE65 and CRALBP were on two gels, respectively). Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/29914), licensed under a CC-BY license.Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) - BSA Free [NB100-355] - Expression of eye-specific markers in the induced eye-like structures induced from lignin-added ES cells. (a) Higher-magnification image of the RPE like structure induced from ESCs after 12-day culture. (b) Immunostaining of eye- like structures. Eye- like structures induced from ESCs after 12-day culture were stained with antibodies against RPE65 (red) and nuclei were stained with DAPI solution (blue). Scale bar: a = 50 um, b,d = 200 um, c,e = 100 um. PLoS One. 2013 Jun 21;8(6):e66376. doi: 10.1371/journal.pone.0066376.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

     7 Reviews

97 Publications
DSPP Antibody - Azide and BSA Free
NBP2-92546
Western Blot: DSPP Antibody [NBP2-92546] - Analysis of lysates from wild type (WT) and 293F cells transfected with DSPP using DSPP Rabbit pAb at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution.Lysates/proteins: 40.5 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit. Exposuretime: 10s.ICC/IF:DSPP Antibody [NBP2-92546] - Analysis of Dental pulp stem cell using DSPP Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ELISA, ICC/IF

3 Publications