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![Western Blot: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM1.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Western-Blot-NBP2-01926-img0022.jpg "Western Blot: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM1.")
![Immunocytochemistry/Immunofluorescence: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM1.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunocytochemistry-NBP2-01926-img0024.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human prostate tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0021.jpg)
![Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - Analysis of Jurkat cells, using anti-LHX1 antibody (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/LIM1-Antibody-OTI2D5-Flow-Cytometry-NBP2-01926-img0025.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0012.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0019.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human Kidney tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0014.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human liver tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0015.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of paraffin-embedded Human pancreas tissue using anti-LIM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Immunohistochemistry-Paraffin-NBP2-01926-img0020.jpg)
![Flow Cytometry: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIM1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/LIM1-Antibody-2D5-Flow-Cytometry-NBP2-01926-img0023.jpg)
![Western Blot: LIM1 Antibody [NBP3-12886] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with LIM1 antibody (NBP3-12886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/LIM1-Antibody-Western-Blot-NBP3-12886-img0007.jpg "Western Blot: LIM1 Antibody [NBP3-12886] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with LIM1 antibody (NBP3-12886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: LIM1 Antibody [NBP3-12886] - LIM1 antibody detects LIM1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. LIM1 antibody (NBP3-12886) dilution: 1:500. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/LIM1-Antibody-Immunohistochemistry-Paraffin-NBP3-12886-img0004.jpg)
![Immunohistochemistry-Frozen: LIM1 Antibody [NBP3-12886] - LIM1 antibody detects LIM1 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: LIM1 protein stained by LIM1 antibody (NBP3-12886) diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/LIM1-Antibody-Immunohistochemistry-Frozen-NBP3-12886-img0002.jpg)
![Immunohistochemistry-Frozen: LIM1 Antibody [NBP3-12886] - LIM1 antibody detects LIM1 protein expression at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: LIM1 protein stained by LIM1 antibody (NBP3-12886) diluted at 1:250. Red: SOX2, stained by SOX2 antibody [GT1876] (NBP2-19304) diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/LIM1-Antibody-Immunohistochemistry-Frozen-NBP3-12886-img0003.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody [NBP3-12886] - LIM1 antibody detects LIM1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.LIM1 stained by LIM1 antibody (NBP3-12886) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-12886_rabbit-lim1-pab-91020242075961.jpg)
![Western Blot: LIM1 Antibody [NBP3-12886] - Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with LIM1 antibody (NBP3-12886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-12886_rabbit-lim1-pab-91020242021976.jpg)
![Immunohistochemistry-Paraffin: LIM1 Antibody [NBP3-12886] - LIM1 antibody detects LIM1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.LIM1 stained by LIM1 antibody (NBP3-12886) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-12886_rabbit-lim1-pab-91020242011508.jpg)
![Immunohistochemistry: LIM1 Antibody - BSA Free [NB110-12933] - Staining using LIM1 antibody - BSA Free](http://images.novusbio.com/fullsize/LIM1-Antibody---BSA-Free-Immunohistochemistry-NB110-12933-img0001.jpg "Immunohistochemistry: LIM1 Antibody - BSA Free [NB110-12933] - Staining using LIM1 antibody - BSA Free")
![Western Blot: LIM1 Antibody [NB100-56626] - Analysis of LIM-1 in muscle lysate with this antibody at 1:500 dilution. A protein band of an approximate molecular weight of 60 kDa was detected.](http://images.novusbio.com/fullsize/LIM1-Antibody-Western-Blot-NB100-56626-img0002.jpg "Western Blot: LIM1 Antibody [NB100-56626] - Analysis of LIM-1 in muscle lysate with this antibody at 1:500 dilution. A protein band of an approximate molecular weight of 60 kDa was detected.")
![Immunocytochemistry/Immunofluorescence: LIM1 Antibody [NBP3-17789] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/LIM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17789-img0001.jpg "Immunocytochemistry/Immunofluorescence: LIM1 Antibody [NBP3-17789] - Staining of human cell line A-431 shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: LIM1 Antibody [NBP2-68955] - Immunohistochemical staining of human kidney shows strong nuclear positivity in subset of cells in tubules.](http://images.novusbio.com/fullsize/LIM1-Antibody-Immunohistochemistry-NBP2-68955-img0001.jpg "Immunohistochemistry-Paraffin: LIM1 Antibody [NBP2-68955] - Immunohistochemical staining of human kidney shows strong nuclear positivity in subset of cells in tubules.")
![Western Blot: LIM1 Overexpression Lysate (Adult Normal) [NBL1-12513] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LIM1.](http://images.novusbio.com/fullsize/LIM1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12513-img0002.jpg "Western Blot: LIM1 Overexpression Lysate (Adult Normal) [NBL1-12513] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LIM1.")