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PAK3 Antibody [Unconjugated]

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Western blot shows lysates of human brain (hypothalamus) tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human PAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6897) followed by ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

  • Size / Catalog#QtyPrice
    AF6897-SP
    25 ug
    $149.00
    5-7 Business Days
    AF6897
    100 ug
    $449.00
    1-3 Business Days
    View Available Formulations
    Catalog# & Formulation Size Price
Shipping $45.00 per order
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PAK3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PAK3
Leu138-Gln255
Accession # O75914
Specificity
Detects human PAK3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) PAK1, rhPAK1B, rhPAK2, rhPAK4, rhPAK6, and rhPAK7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
PAK3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PAK3 Antibody [Unconjugated]

  • beta-PAK
  • bPAK
  • CDKN1A
  • EC 2.7.11
  • EC 2.7.11.1
  • hPAK3
  • mental retardation, X-linked 47
  • MRX30
  • MRX47
  • oligophrenin-3
  • OPHN3
  • p21 (CDKN1A)-activated kinase 3
  • p21 protein (Cdc42/Rac)-activated kinase 3
  • p21-activated kinase 3
  • PAK3
  • PAK-3
  • PAK3beta
  • serine/threonine-protein kinase PAK 3

Background

PAK3 (p21-activaed kinase 3; also beta -PAK, PAK3b and oligophrenin-3) is a cytoplasmic 68 kDa member of PAK group I, STE20 subfamily, STE Ser/Thr protein kinase family of molecules. It is expressed in neurons of cortical layers II, III and V, the arcuate nucleus, and neurons in the dorsal raphe plus locus coeruleus. PAK3 mediates axon and dentrite arborization, providing volume to the mass of the CNS. It binds to active GTPases which induce PAK autophosphorylation and activation. Human PAK3(b) is 559 amino acids (aa) in length. It contains one GTPase binding domain (aa 68-128) plus a protein kinase catalytic region (aa 283-534). Acetylation occurs on Lys535, and there are at least three potential phosphorylation sites. Alternate splicing generates three isoform variants. There is a 65 kDa short form that shows a deletion of aa 93-107 (PAK3a), a 69 kDa long form that shows a 21 aa substitution for aa 93-107 (PAK3c), and a 72 kDa very long isoform that contains the aforementioned 21 aa "substitution" inserted onto PAK3b after Thr92 (PAK3cb). PAK3b, c and cb are all constitutively active, and show only modest binding affinity towards GTPases. Over aa 138-255, human PAK3(b) shares 94% aa identity with mouse PAK3(b).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PAK3
Uniprot