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![Western Blot: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Western blot analysis of VDR/NR1I1/Vitamin D Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: U937 cell lysate](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-JA11-16-Western-Blot-NBP2-66778-img0008.jpg "Western Blot: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Western blot analysis of VDR/NR1I1/Vitamin D Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate Lane 2: U937 cell lysate")
![Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded rat skin tissue with Rabbit anti-Vitamin D Receptor antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-JA11-16-Immunohistochemistry-Paraffin-NBP2-66778-img0010.jpg)
![Flow Cytometry: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of Hela cells with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-JA11-16-Flow-Cytometry-NBP2-66778-img0001.jpg)
![Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded mouse skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-JA11-16-Immunohistochemistry-Paraffin-NBP2-66778-img0006.jpg)
![Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded mouse colon tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-JA11-16-Immunohistochemistry-Paraffin-NBP2-66778-img0005.jpg)
![Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded human skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-JA11-16-Immunohistochemistry-Paraffin-NBP2-66778-img0004.jpg)
![Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded rat colon tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-JA11-16-Immunohistochemistry-Paraffin-NBP2-66778-img0007.jpg)
![Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Vitamin D Receptor antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-JA11-16-Immunohistochemistry-Paraffin-NBP2-66778-img0009.jpg)
![Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (2F4) [H00007421-M02] - Analysis of monoclonal antibody to VDR on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-2F4-Immunohistochemistry-Paraffin-H00007421-M02-img0006.jpg "Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (2F4) [H00007421-M02] - Analysis of monoclonal antibody to VDR on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.")
![Sandwich ELISA: VDR/NR1I1/Vitamin D Receptor Antibody (2F4) [H00007421-M02] - Detection limit for recombinant GST tagged VDR is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-2F4-Sandwich-ELISA-H00007421-M02-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: VDR/NR1I1/Vitamin D Receptor Antibody [NBP2-98841] - Immunofluorescence staining of VDR/NR1I1/Vitamin D Receptor in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human VDR/NR1I1/Vitamin D Receptor polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red).Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98841-img0001.jpg "Immunocytochemistry/Immunofluorescence: VDR/NR1I1/Vitamin D Receptor Antibody [NBP2-98841] - Immunofluorescence staining of VDR/NR1I1/Vitamin D Receptor in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human VDR/NR1I1/Vitamin D Receptor polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red).Positive staining was localized to nucleus.")
![Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody [NBP2-98841] - Staining of human VDR/NR1I1/Vitamin D Receptor in human small intestine with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-Immunohistochemistry-Paraffin-NBP2-98841-img0003.jpg)
![Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody [NBP2-98841] - Staining of human VDR/NR1I1/Vitamin D Receptor in human colon with rabbit polyclonal antibody (1:1000).](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-Immunohistochemistry-Paraffin-NBP2-98841-img0002.jpg)
![Immunohistochemistry: VDR/NR1I1/Vitamin D Receptor Antibody (3C7) [NBP3-26255] - Image of VDR/NR1I1/Vitamin D Receptor Antibody (3C7) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26255_rabbit-vdr-nr1i1-vitamin-d-receptor-mab-3c7-27220248313711.jpg "Immunohistochemistry: VDR/NR1I1/Vitamin D Receptor Antibody (3C7) [NBP3-26255] - Image of VDR/NR1I1/Vitamin D Receptor Antibody (3C7) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: VDR/NR1I1/Vitamin D Receptor Antibody (3C7) [NBP3-26255] - Positive Western Blot detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, HL-60 whole cell lysate. All lanes: VDR/NR1I1/Vitamin D Receptor Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 49, 54 kDa Observed band size: 49 kDa](http://images.novusbio.com/fullsize/nbp3-26255_rabbit-vdr-nr1i1-vitamin-d-receptor-mab-3c7-282202411485838.jpg)
![Western Blot: VDR/NR1I1/Vitamin D Receptor Antibody [NB600-794] - Staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-Western-Blot-NB600-794-img0001.jpg "Western Blot: VDR/NR1I1/Vitamin D Receptor Antibody [NB600-794] - Staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: VDR/NR1I1/Vitamin D Receptor Antibody [NBP2-55786] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55786-img0001.jpg "Immunocytochemistry/Immunofluorescence: VDR/NR1I1/Vitamin D Receptor Antibody [NBP2-55786] - Staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.")
![ELISA: Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric) [NBP2-82389] - Samples were spiked with high concentrations of Human VDR/NR1I1/Vitamin D Receptor and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82389_human-vdr-nr1i1-vitamin-d-receptor-elisa-kit-colorimetric-13220241613072.jpg "ELISA: Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric) [NBP2-82389] - Samples were spiked with high concentrations of Human VDR/NR1I1/Vitamin D Receptor and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human VDR/NR1I1/Vitamin D Receptor ELISA Kit (Colorimetric) [NBP2-82389] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82389_human-vdr-nr1i1-vitamin-d-receptor-elisa-kit-colorimetric-4320248582557.jpg)
![Western Blot: Vitamin D Receptor Overexpression Lysate (Adult Normal) [NBL1-17711] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Vitamin D Receptor.](http://images.novusbio.com/fullsize/Vitamin-D-Receptor-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17711-img0002.jpg "Western Blot: Vitamin D Receptor Overexpression Lysate (Adult Normal) [NBL1-17711] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Vitamin D Receptor.")
![Western Blot: Vitamin D Receptor Overexpression Lysate (Adult Normal) [NBP2-11216] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Vitamin D Receptor.](http://images.novusbio.com/fullsize/Vitamin-D-Receptor-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11216-img0001.jpg "Western Blot: Vitamin D Receptor Overexpression Lysate (Adult Normal) [NBP2-11216] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Vitamin D Receptor.")
![Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody [NBP2-98840] - Immunochemical staining of human VDR/NR1I1/Vitamin D Receptor in human pancreas with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/VDR-NR1I1-Vitamin-D-Receptor-Antibody-Immunohistochemistry-Paraffin-NBP2-98840-img0001.jpg "Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody [NBP2-98840] - Immunochemical staining of human VDR/NR1I1/Vitamin D Receptor in human pancreas with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).")