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![Knockout Validated: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TJP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:200 of Rabbit Anti-Human TJP1 Polyclonal Antibody (Catalog # NBP1-85047) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TJP1 at approximately 260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Knockout-Validated-NBP1-85047-img0019.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TJP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:200 of Rabbit Anti-Human TJP1 Polyclonal Antibody (Catalog # NBP1-85047) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TJP1 at approximately 260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining in human testis and skeletal muscle tissues. Corresponding TJP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0018.jpg)
![Simple Western: Tight Junction Protein 1 Antibody [NBP1-85047] - Simple Western lane view shows a specific band for TJP1 in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Simple-Western-NBP1-85047-img0006.jpg)
![Immunohistochemistry: Tight Junction Protein 1 Antibody [NBP1-85047] - Representative images of DCFA excretion at the biliary poles of corr-FH-iHeps. Left image FH-i, right image Corr-FH-iHeps. All images were taken with 10x objective, z-stacks of xy sections of the cells were acquired with an epifluorescence microscope (Nikon Elipse) and analyzed with ImageJ software. Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-019-1342-6) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-NBP1-85047-img0025.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Effects of dietary Flammulina velutipes stem waste (FVS) inclusion on the relative expression of tight junction proteins in jejunum of growing pigs. a The relative expression of ZO-1 in jejunum of growing pigs fed control and 2.5% or 5% FVS diets. Values are means (3 pigs per treatment) with standard errors represented by vertical bars. *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following publication (//jasbsci.biomedcentral.com/articles/10.1186/s40104-020-00449-y) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85047-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Rat epithelial cells stained positively with Tight Junction Protein 1. Primary antibody at 1:100. Secondary antibody: goat anti-rabbit-Alexa 488 conjugated at 1:500. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0024.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Analysis in control (vector only transfected HEK293T lysate) and TJP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85047-img0020.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85047-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of Pig trabecular meshwork cells. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human cell line U-2 OS shows localization to cytosol & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - The primary mouse lung endothelial cells were fixed, permeabilized and stained with 1:100 diluted anti-ZO-1 ab for overnight. Samples were washed and subsequently incubated with 1:500 diluted Alexa Fuor 546 at room temperature for 1 hour. ICC/iF image submitted by a verified customer reveiw.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Monolayer of Human ARPE-19 cells on a glass substrate. Tight Junction Protein 1 staining in red. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0023.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0014.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0015.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0016.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human testis shows moderate to strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0017.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot analysis of function protein expression on primary human renal cortical epithelial cells challenged with cytokines. Primary human renal tubular epithelial cells are challenged with cytokine cocktail (15nM IFN gamma , 6nM TNF alpha , & 3nM IL1 beta ). (A, B) Expression of Snail, E-Cad, TJP1, SMA. AhR, IDO, KMO, KY, MHCI & II. (C, D) Densitometric quantitation of protein in (A, B). All Western blots are representative of at least three independent experiments. *P < 0.05, ***P < 0.0001 versus to cells without treatment, analysis is multiple t-tests, pairwise comparison of individual time-point to control indicated same p-value. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-310202415384165.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Effects of dietary Flammulina velutipes stem waste (FVS) inclusion on the relative expression of tight junction proteins in jejunum of growing pigs. a The relative expression of ZO-1 in jejunum of growing pigs fed control & 2.5% or 5% FVS diets. b The relative expression of occludin in jejunum of growing pigs fed control & 2.5% or 5% FVS diets. c The relative expression of claudin-1 in jejunum of growing pigs fed control & 2.5% or 5% FVS diets. Values are means (3 pigs per treatment) with standard errors represented by vertical bars. *P < 0.05, **P < 0.01 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32391146), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-310202415395958.jpg)
![Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Differentiation of FH- & corr-FH-iPSCs into hepatocytes. a Representative pictures of cell morphology & immunostainings of the indicated markers at day 25 of differentiation. Scale bars: 50 μm. b Representative images of DCFA excretion at the biliary poles of corr-FH-iHeps. All images were taken with × 10 objective, z-stacks of xy sections of the cells were acquired with an epifluorescence microscope (Nikon Elipse) & analyzed with ImageJ software. Arrowheads indicate bile canaliculi Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31358055), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-31020241539255.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Protective role of 3HK & 3HAA in TEC stimulated with cytokines. Primary human renal TEC pre-incubated with different doses of 3HK or 3HAA overnight; the cells are challenged with the cytokine cocktail (15 nM IFN gamma , 6 nM TNF alpha , & 3 nM IL1 beta ) for 24 h; protein expression was assayed with Western blot: (A, B) 3HK & 3HAA cannot reverse IDO, MHC I, BID expression induced by cytokines. 3HK & 3HAA effectively restore Bcl-xL & Tjp1 expression. 3HAA shows its different function in up-regulation of AhR expression in TEC in inflammatory conditions. (C) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HK. ***P < 0.0001 versus cell treated with Cyto. (D) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HAA. ***P < 0.0001, ****P < 0.00001 versus cell treated with Cyto. Pairwise comparison of individual dose to control indicated same p-value. All Western blots are representative of three independent experiments; analysis is multiple one-way ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-3102024161362.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Protective role of 3HK & 3HAA in TEC stimulated with cytokines. Primary human renal TEC pre-incubated with different doses of 3HK or 3HAA overnight; the cells are challenged with the cytokine cocktail (15 nM IFN gamma , 6 nM TNF alpha , & 3 nM IL1 beta ) for 24 h; protein expression was assayed with Western blot: (A, B) 3HK & 3HAA cannot reverse IDO, MHC I, BID expression induced by cytokines. 3HK & 3HAA effectively restore Bcl-xL & Tjp1 expression. 3HAA shows its different function in up-regulation of AhR expression in TEC in inflammatory conditions. (C) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HK. ***P < 0.0001 versus cell treated with Cyto. (D) Qualification of protein expression in TEC challenged with cytokine cocktail in the presence of 3HAA. ***P < 0.0001, ****P < 0.00001 versus cell treated with Cyto. Pairwise comparison of individual dose to control indicated same p-value. All Western blots are representative of three independent experiments; analysis is multiple one-way ANOVA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34305900), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85047_rabbit-polyclonal-tight-junction-protein-1-antibody-310202416114731.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining in human cerebral cortex and skeletal muscle tissues using anti-TJP1 antibody. Corresponding TJP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85046-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining in human cerebral cortex and skeletal muscle tissues using anti-TJP1 antibody. Corresponding TJP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046] - Analysis of Tight Junction Protein 1 in RIPA whole cell lysates of mouse primary choroid plexus epithelial cells using anti-Tight Junction Protein 1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85046-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining of human cell line U-2 OS shows localization to cytosol & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85046-img0007.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85046-img0011.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85046-img0012.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046] - Expression of tight junction proteins in cerebellum of rats with mild liver damage. Expression of (A,B) occludin & (C,D) ZO-1 at 2 & 4 weeks was analyzed by Western blot. Values are mean ± SEM of 10–12 rats per group. One-way ANOVA with Tukey’s test was performed for occludin at 2 weeks (F(3,27) = 4.99, p 0.05) & for ZO-1 at 2 weeks (W(3,23) = 0.715, p > 0.05) & 4 weeks (F(3,43) = 3.698, p 0.05) & for ZO-1 at 2 weeks (W(3,23) = 0.715, p > 0.05) & 4 weeks (F(3,43) = 3.698, p < 0.05). Values significantly different from control rats are indicated by asterisks & from CCl4 rats by a. * p < 0.05; a p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-85046_rabbit-polyclonal-tight-junction-protein-1-antibody-31020241535626.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Detection of human TJP1/ZO-1 by WB of A-549, K-562, HEK293T, MCF-7, Jurkat, HeLa, LNCaP, and RKO lysate. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal [BLR092G] (NBP2-80141). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Western-Blot-NBP2-80141-img0006.jpg "Western Blot: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Detection of human TJP1/ZO-1 by WB of A-549, K-562, HEK293T, MCF-7, Jurkat, HeLa, LNCaP, and RKO lysate. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal [BLR092G] (NBP2-80141). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Rabbit anti-ZO-1 recombinant monoclonal antibody [BLR092G]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Immunocytochemistry-Immunofluorescence-NBP2-80141-img0007.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Rabbit anti-ZO-1 recombinant monoclonal antibody [BLR092G]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Immunohistochemistry-Paraffin-NBP2-80141-img0009.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Detection of mouse TJP1/ZO-1 by WB of mIMCD-3, RenCa, NIH 3T3, TCMK-1, and C2C12 lysate. Antibody: Rabbit anti-TJP1/ZO-1 recombinant monoclonal [BLR092G] (A700-092). Secondary: HRP-conjugated goat anti-rabbit IgG (NBP2-80141). Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Western-Blot-NBP2-80141-img0005.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Rabbit anti-ZO-1 recombinant monoclonal antibody [BLR092G]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Immunohistochemistry-Paraffin-NBP2-80141-img0008.jpg)
![Immunoprecipitation: Tight Junction Protein 1 Antibody (BLR092G) [NBP2-80141] - Detection of human TJP1/ZO-1 by WB immunoprecipitates from A-549 lysate. Antibodies: Rabbit anti-TJP1/ZO-1 recombinant monoclonal [BLR092G] (NBP2-80141) and another rabbit anti-TJP1/ZO-1.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-BLR092G-Immunoprecipitation-NBP2-80141-img0001.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-212024936270.jpg "Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Knockout Validated: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Wild-type (WT) and TJP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-2120249374943.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-2120249415937.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-212024940839.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - ZO-1 antibody [HL1185] detects ZO-1 protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZO-1 stained by ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-2120249432869.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (HL1185) - Azide and BSA Free [NBP3-25774] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1185] (NBP3-25774) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25774_rabbit-tight-junction-protein-1-mab-hl1185-azide-bsa-free-2120249371525.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP2-21051-img0009.jpg "Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP2-21051] - Tight Junction Protein 1 Antibody Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: TJP1 protein stained by ZO-1 antibody, Internal diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-21051-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP2-21051-img0005.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Tight Junction Protein 1 antibody [N2C1], Internal (NBP2-21051) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-21051_rabbit-polyclonal-tight-junction-protein-1-antibody-91020242013403.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cellular junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-46111_goat-polyclonal-tight-junction-protein-1-antibody-imgenex-img-31199-187202311342910.jpg "Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cellular junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-46111_goat-polyclonal-tight-junction-protein-1-antibody-imgenex-img-31199-187202311342922.jpg)
![Western Blot: Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free [NBP3-25773] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1133] (NBP3-25773) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25773_rabbit-tight-junction-protein-1-mab-hl1133-azide-bsa-free-2120249362714.jpg "Western Blot: Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free [NBP3-25773] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1133] (NBP3-25773) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free [NBP3-25773] - ZO-1 antibody [HL1133] detects ZO-1 protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZO-1 stained by ZO-1 antibody [HL1133] (NBP3-25773) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25773_rabbit-tight-junction-protein-1-mab-hl1133-azide-bsa-free-2120249342790.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Tight Junction Protein 1 Antibody (HL1133) - Azide and BSA Free [NBP3-25773] - Wild-type (WT) and TJP1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [HL1133] (NBP3-25773) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25773_rabbit-tight-junction-protein-1-mab-hl1133-azide-bsa-free-2120249413369.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP1-91621] - Tight Junction Protein 1 Antibody Rat Lung Lysate 1ug/ml Gel Concentration 6-18%](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-91621-img0002.jpg "Western Blot: Tight Junction Protein 1 Antibody [NBP1-91621] - Tight Junction Protein 1 Antibody Rat Lung Lysate 1ug/ml Gel Concentration 6-18%")
![Western Blot: Tight Junction Protein 1 Overexpression Lysate (Native) [NBP2-10877] - Tight Junction Protein 1 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZO-1 tight junction protein.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Overexpression-Lysate-Native-Western-Blot-NBP2-10877-img0002.jpg "Western Blot: Tight Junction Protein 1 Overexpression Lysate (Native) [NBP2-10877] - Tight Junction Protein 1 Overexpression Lysate (Native) Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZO-1 tight junction protein.")