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![TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 acts through formation of heterodimer complexes with TLR1 or TLR6. HEK 293 endogenously expresses TLR1 and TLR6, so that the TLR2 reporter cell line can response to both Pam3CSK4 (TLR2/TLR1 specific ligand) and MALP-2 (TLR2/TLR6 specific ligand). The TLR2 / TLR4 inhibitor specifically inhibits TLR2/TLR1 receptor complex activity in a dose-response manner but exhibited no or little effect on TLR2/TLR6 receptor complex activity.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-NBP2-26245-img0007.jpg "TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 acts through formation of heterodimer complexes with TLR1 or TLR6. HEK 293 endogenously expresses TLR1 and TLR6, so that the TLR2 reporter cell line can response to both Pam3CSK4 (TLR2/TLR1 specific ligand) and MALP-2 (TLR2/TLR6 specific ligand). The TLR2 / TLR4 inhibitor specifically inhibits TLR2/TLR1 receptor complex activity in a dose-response manner but exhibited no or little effect on TLR2/TLR6 receptor complex activity.")
![TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - The TLR4 reporter cell line responds to LPS. The TLR2 / TLR4 inhibitor specifically inhibits TLR4 activation upon LPS stimulation in a dose-response manner.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-NBP2-26245-img0008.jpg)
![TIRAP (TLR2 and TLR4) Inhibitor Peptide [NBP2-26245] - TLR2 /4 Inhibitor suppressed the Pam3CSK4-induced TLR2/TLR1 activity [A] and the LPS-induced TLR4 activity [B] in a dose-response manner, of which IC50 values were measured as 17.2 uM and 10.47 uM, respectively.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-NBP2-26245-img0009.jpg)
![Functional (Inhibition): TIRAP (TLR2 and TLR4) Inhibitor Peptide Set [NBP2-29331] - TLR2/NF-kB/SEAPorter HEK 293 (NBP2-26274) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29331) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Pam3CSK4 (NBP2-25297) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Inhibitor-Peptide-Set-Functional-Inhibition-NBP2-29331-img0001.jpg "Functional (Inhibition): TIRAP (TLR2 and TLR4) Inhibitor Peptide Set [NBP2-29331] - TLR2/NF-kB/SEAPorter HEK 293 (NBP2-26274) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29331) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Pam3CSK4 (NBP2-25297) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).")
![Western Blot: TIRAP (TLR2 and TLR4) Antibody [NB300-990] - (1 ug/ml) staining of human liver lysate 35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TIRAP-TLR2-and-TLR4-Antibody-Western-Blot-NB300-990-img0005.jpg "Western Blot: TIRAP (TLR2 and TLR4) Antibody [NB300-990] - (1 ug/ml) staining of human liver lysate 35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: TIRAP Antibody (20D1055.1) [NB100-56730] - Analysis of TIRAP in human spleen lysate using this antibody.](http://images.novusbio.com/fullsize/TIRAP-Antibody-20D1055-1-Western-Blot-NB100-56730-img0003.jpg "Western Blot: TIRAP Antibody (20D1055.1) [NB100-56730] - Analysis of TIRAP in human spleen lysate using this antibody.")
![Western Blot: TIRAP Antibody (9T1A8) [NBP3-16854] - Western blot analysis of extracts of various cell lines, using TIRAP Rabbit mAb (NBP3-16854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/TIRAP-Antibody-9T1A8-Western-Blot-NBP3-16854-img0003.jpg "Western Blot: TIRAP Antibody (9T1A8) [NBP3-16854] - Western blot analysis of extracts of various cell lines, using TIRAP Rabbit mAb (NBP3-16854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: TIRAP Antibody (9T1A8) [NBP3-16854] - Immunohistochemistry of paraffin-embedded mouse kidney using TIRAP Rabbit mAb (NBP3-16854) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TIRAP-Antibody-9T1A8-Immunohistochemistry-Paraffin-NBP3-16854-img0002.jpg)
![Immunohistochemistry-Paraffin: TIRAP Antibody (9T1A8) [NBP3-16854] - Immunohistochemistry of paraffin-embedded rat lung using TIRAP Rabbit mAb (NBP3-16854) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TIRAP-Antibody-9T1A8-Immunohistochemistry-Paraffin-NBP3-16854-img0001.jpg)
![Western Blot: TIRAP Antibody [NBP2-95138] - Analysis of extracts of various cell lines, using TIRAP at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/TIRAP-Antibody-Western-Blot-NBP2-95138-img0005.jpg "Western Blot: TIRAP Antibody [NBP2-95138] - Analysis of extracts of various cell lines, using TIRAP at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: TIRAP Antibody [NBP2-95138] - Analysis of U2OS cells using TIRAP at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/TIRAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-95138-img0001.jpg)
![Western Blot: TIRAP Antibody [NB100-2823] - Staining (0.3µg/ml) of A549 (A) and K562 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TIRAP-Antibody-Western-Blot-NB100-2823-img0004.jpg "Western Blot: TIRAP Antibody [NB100-2823] - Staining (0.3µg/ml) of A549 (A) and K562 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot: TIRAP Antibody [NB100-2823] - Staining (0.3ug/ml) of Human colorectal cancer lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TIRAP-Antibody-Western-Blot-NB100-2823-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TIRAP Antibody [NBP2-47605] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, cytosol & cytokinetic bridge.](http://images.novusbio.com/fullsize/TIRAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47605-img0004.jpg "Immunocytochemistry/Immunofluorescence: TIRAP Antibody [NBP2-47605] - Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm, cytosol & cytokinetic bridge.")
![Immunohistochemistry-Paraffin: TIRAP Antibody [NBP2-47605] - Staining of human gallbladder shows cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/TIRAP-Antibody-Immunohistochemistry-Paraffin-NBP2-47605-img0003.jpg)
![Immunohistochemistry-Paraffin: TIRAP Antibody [NBP3-04543] - Paraffin-embedded human liver cancer using TIRAP antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/TIRAP-Antibody-Immunohistochemistry-Paraffin-NBP3-04543-img0001.jpg "Immunohistochemistry-Paraffin: TIRAP Antibody [NBP3-04543] - Paraffin-embedded human liver cancer using TIRAP antibody at dilution of 1:200 (40x lens).")
![Immunohistochemistry-Paraffin: TIRAP Antibody [NBP3-04543] - Paraffin-embedded human colon carcinoma using TIRAP antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/TIRAP-Antibody-Immunohistochemistry-Paraffin-NBP3-04543-img0002.jpg)
![Western Blot: TIRAP Antibody (SR1476) [NBP3-22201] - Western blot analysis using NBP3-22201 in HUVEC cell lysate.](http://images.novusbio.com/fullsize/nbp3-22201_rabbit-tirap-mab-sr1476-57202317161870.jpg "Western Blot: TIRAP Antibody (SR1476) [NBP3-22201] - Western blot analysis using NBP3-22201 in HUVEC cell lysate.")
![Western Blot: TIRAP Antibody (JE46-35) [NBP3-33024] - Western blot analysis of TIRAP on different lysates with Rabbit anti-TIRAP antibody (NBP3-33024) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: U-2 OS cell lysate (20 ug/Lane) Lane 3: RAW264.7 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Predicted band size: 24 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33024) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33024_rabbit-tirap-mab-je46-35-288202412493424.jpg "Western Blot: TIRAP Antibody (JE46-35) [NBP3-33024] - Western blot analysis of TIRAP on different lysates with Rabbit anti-TIRAP antibody (NBP3-33024) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: U-2 OS cell lysate (20 ug/Lane) Lane 3: RAW264.7 cell lysate (20 ug/Lane) Lane 4: Mouse liver tissue lysate (40 ug/Lane) Predicted band size: 24 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33024) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")