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![Western Blot: PUM2 Antibody [NB100-387] - Functionality of products expressed from miRNA134. 1, 2 and miRNA99a.1. miR-134 expressed from the miRNA134.1 construct affects the Pum2 protein levels. 10-11 DIV hippocampal neurons were transduced with purified rAAV carrying the indicated plasmids. Eight days later whole cell extracts were prepared and subjected to western blot analysis probing with Pum2 antibody and a beta-actin antibody as loading control. Here we show one out of three representative blots, on average displaying a significant 64% reduction in Pum2 protein levels. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/neuro.04.016.2009/abstract), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NB100-387-img0003.jpg "Western Blot: PUM2 Antibody [NB100-387] - Functionality of products expressed from miRNA134. 1, 2 and miRNA99a.1. miR-134 expressed from the miRNA134.1 construct affects the Pum2 protein levels. 10-11 DIV hippocampal neurons were transduced with purified rAAV carrying the indicated plasmids. Eight days later whole cell extracts were prepared and subjected to western blot analysis probing with Pum2 antibody and a beta-actin antibody as loading control. Here we show one out of three representative blots, on average displaying a significant 64% reduction in Pum2 protein levels. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/neuro.04.016.2009/abstract), licensed under a CC-BY license.")
![Western Blot: PUM2 Antibody [NB100-387] - Detection of Human and Mouse Pumilio 2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Pumilio 2 antibody NB100-387 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NB100-387-img0002.jpg)
![Western Blot: PUM2 Antibody [NBP1-89623] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NBP1-89623-img0012.jpg "Western Blot: PUM2 Antibody [NBP1-89623] - Analysis in human cell line HEL.")
![Immunocytochemistry/Immunofluorescence: PUM2 Antibody [NBP1-89623] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PUM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89623-img0011.jpg)
![Immunohistochemistry-Paraffin: PUM2 Antibody [NBP1-89623] - Staining of human thyroid gland shows distinct cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PUM2-Antibody-Immunohistochemistry-Paraffin-NBP1-89623-img0010.jpg)
![Western Blot: PUM2 Antibody [NBP2-20009] - Sample (20 ug of whole cell lysate) A: Human ESC 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NBP2-20009-img0001.jpg "Western Blot: PUM2 Antibody [NBP2-20009] - Sample (20 ug of whole cell lysate) A: Human ESC 5% SDS PAGE gel, diluted at 1:2000.")
![Immunocytochemistry/Immunofluorescence: PUM2 Antibody [NBP2-20009] - Pumilio 2 antibody detects Pumilio 2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Pumilio 2 stained by Pumilio 2 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/PUM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20009-img0003.jpg)
![Western Blot: PUM2 Antibody [NBP2-20009] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Pumilio 2 antibody [C2C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NBP2-20009-img0002.jpg)
![Immunohistochemistry-Paraffin: PUM2 Antibody [NBP2-20010] - Paraffin-embedded mouse kidney PUM2 Antibody diluted at 1:500.](http://images.novusbio.com/fullsize/PUM2-Antibody-Immunohistochemistry-Paraffin-NBP2-20010-img0009.jpg "Immunohistochemistry-Paraffin: PUM2 Antibody [NBP2-20010] - Paraffin-embedded mouse kidney PUM2 Antibody diluted at 1:500.")
![Western Blot: PUM2 Antibody [NBP2-20010] - Sample (20 ug of whole cell lysate) A: human ESC 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NBP2-20010-img0006.jpg)
![Western Blot: PUM2 Antibody [NBP2-20010] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PUM2 antibody [N1N2], N-term (NBP2-20010) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-20010_rabbit-polyclonal-pum2-antibody-910202420115061.jpg)
![Western Blot: PUM2 Antibody (SR1070) [NBP3-22199] - Western blot analysis using NBP3-22199 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-22199_rabbit-pum2-mab-sr1070-5720231512348.jpg "Western Blot: PUM2 Antibody (SR1070) [NBP3-22199] - Western blot analysis using NBP3-22199 in 293T cell lysate.")
![Western Blot: PUM2 Antibody [NBP2-56823] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/PUM2-Antibody-Western-Blot-NBP2-56823-img0002.jpg "Western Blot: PUM2 Antibody [NBP2-56823] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: PUM2 Antibody [NBP2-56823] - Staining of human cell line A-431 shows localization to nuclear membrane & cytosol.](http://images.novusbio.com/fullsize/PUM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56823-img0001.jpg)
![Western Blot: PUM2 Antibody (7M1B1) [NBP3-15913] - Western blot analysis of extracts of Rat testis, using PUM2 antibody (NBP3-15913) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/PUM2-Antibody-7M1B1-Western-Blot-NBP3-15913-img0001.jpg "Western Blot: PUM2 Antibody (7M1B1) [NBP3-15913] - Western blot analysis of extracts of Rat testis, using PUM2 antibody (NBP3-15913) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: PUM2 Antibody (7M1B1) [NBP3-15913] - Western blot analysis of extracts of various cell lines, using PUM2 antibody (NBP3-15913) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/PUM2-Antibody-7M1B1-Western-Blot-NBP3-15913-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PUM2 Antibody [NBP2-97161] - Immunofluorescence staining of PUM2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PUM2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/PUM2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97161-img0001.jpg "Immunocytochemistry/Immunofluorescence: PUM2 Antibody [NBP2-97161] - Immunofluorescence staining of PUM2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PUM2 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: PUM2 Antibody [NBP2-97161] - Staining of human PUM2 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PUM2-Antibody-Immunohistochemistry-Paraffin-NBP2-97161-img0002.jpg)
![Western Blot: PUM2 Antibody (JE37-76) [NBP3-32879] - Western blot analysis of PUM2 on different lysates with Rabbit anti-PUM2 antibody (NBP3-32879) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 114 kDa Observed band size: 140 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32879) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32879_rabbit-pum2-mab-je37-76-288202412461618.jpg "Western Blot: PUM2 Antibody (JE37-76) [NBP3-32879] - Western blot analysis of PUM2 on different lysates with Rabbit anti-PUM2 antibody (NBP3-32879) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 114 kDa Observed band size: 140 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32879) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: PUM2 Antibody (JE37-76) [NBP3-32879] - Flow cytometric analysis of U-2 OS cells labeling PUM2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32879, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32879_rabbit-pum2-mab-je37-76-392024884690.jpg)
![Western Blot: Pumilio 2 Overexpression Lysate (Adult Normal) [NBP2-10289] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pumilio 2.](http://images.novusbio.com/fullsize/Pumilio-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10289-img0001.jpg "Western Blot: Pumilio 2 Overexpression Lysate (Adult Normal) [NBP2-10289] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pumilio 2.")