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![Western Blot: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of SHP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Western-Blot-NBP2-67321-img0010.jpg "Western Blot: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of SHP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate")
![Immunocytochemistry/Immunofluorescence: SHP-1 Antibody (SR41-02) [NBP2-67321] - Staining SHP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunocytochemistry-Immunofluorescence-NBP2-67321-img0003.jpg)
![Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-SHP-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunohistochemistry-Paraffin-NBP2-67321-img0011.jpg)
![Flow Cytometry: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of Hela cells with SHP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Flow-Cytometry-NBP2-67321-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SHP-1 Antibody (SR41-02) [NBP2-67321] - Staining SHP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunocytochemistry-Immunofluorescence-NBP2-67321-img0002.jpg)
![Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded human tonsil tissue using anti-SHP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunohistochemistry-Paraffin-NBP2-67321-img0004.jpg)
![Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded mouse brain tissue using anti-SHP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunohistochemistry-Paraffin-NBP2-67321-img0005.jpg)
![Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded mouse liver tissue using anti-SHP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunohistochemistry-Paraffin-NBP2-67321-img0006.jpg)
![Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded mouse spleen tissue using anti-SHP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunohistochemistry-Paraffin-NBP2-67321-img0007.jpg)
![Immunohistochemistry-Paraffin: SHP-1 Antibody (SR41-02) [NBP2-67321] - Analysis of paraffin-embedded rat brain tissue using anti-SHP1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/SHP-1-Antibody-SR41-02-Immunohistochemistry-Paraffin-NBP2-67321-img0008.jpg)
![Western Blot: PTPN6 Antibody [NBP1-32179] - Sample (30 ug of whole cell lysate) A:NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PTPN6-Antibody-Western-Blot-NBP1-32179-img0003.jpg "Western Blot: PTPN6 Antibody [NBP1-32179] - Sample (30 ug of whole cell lysate) A:NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunoprecipitation: PTPN6 Antibody [NBP1-32179] - HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of SHP1 protein expressing HeLa cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of SHP1 by 3 ug of SHP1 7.5% SDS-PAGE The immunoprecipitated SHP1 protein was detected by SHP1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/PTPN6-Antibody-Immunoprecipitation-NBP1-32179-img0008.jpg)
![Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] - SHP-1 antibody detects SHP-1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.SHP-1 stained by SHP-1 antibody (NBP1-32179) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32179_rabbit-polyclonal-shp-1-antibody-9102024209147.jpg)
![Western Blot: SHP-1 Antibody [NBP1-32179] - Various whole cell extracts (10 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP-1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32179_rabbit-polyclonal-shp-1-antibody-91020242014651.jpg)
![Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] - SHP-1 antibody detects SHP-1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.SHP-1 stained by SHP-1 antibody (NBP1-32179) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32179_rabbit-polyclonal-shp-1-antibody-91020242014652.jpg)
![Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] - SHP-1 antibody detects SHP-1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.SHP-1 stained by SHP-1 antibody (NBP1-32179) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32179_rabbit-polyclonal-shp-1-antibody-91020242014668.jpg)
![Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] - SHP-1 antibody detects SHP-1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.SHP-1 stained by SHP-1 antibody (NBP1-32179) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32179_rabbit-polyclonal-shp-1-antibody-910202420115088.jpg)
![Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] - SHP-1 antibody detects SHP-1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.SHP-1 stained by SHP-1 antibody (NBP1-32179) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32179_rabbit-polyclonal-shp-1-antibody-910202420115048.jpg)
![Western Blot: SHP-1 Antibody [NBP1-32179] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP-1 antibody (NBP1-32179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32179_rabbit-polyclonal-shp-1-antibody-910202420104499.jpg)
![Western Blot: SHP-1 Antibody (7I1K3) [NBP3-15669] - Western blot analysis of extracts of Raji cells, using SHP-1 antibody (NBP3-15669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/SHP-1-Antibody-7I1K3-Western-Blot-NBP3-15669-img0006.jpg "Western Blot: SHP-1 Antibody (7I1K3) [NBP3-15669] - Western blot analysis of extracts of Raji cells, using SHP-1 antibody (NBP3-15669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry: SHP-1 Antibody (7I1K3) [NBP3-15669] - Immunofluorescence analysis of mouse spleen using SHP-1 Rabbit mAb (NBP3-15669) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SHP-1-Antibody-7I1K3-Immunohistochemistry-NBP3-15669-img0005.jpg)
![Western Blot: SHP-1 Antibody (7I1K3) [NBP3-15669] - Western blot analysis of extracts of various cell lines, using SHP-1 antibody (NBP3-15669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/SHP-1-Antibody-7I1K3-Western-Blot-NBP3-15669-img0007.jpg)
![Immunohistochemistry-Paraffin: SHP-1 Antibody (7I1K3) [NBP3-15669] - Immunohistochemistry of paraffin-embedded rat spleen using SHP-1 Rabbit mAb (NBP3-15669) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SHP-1-Antibody-7I1K3-Immunohistochemistry-Paraffin-NBP3-15669-img0001.jpg)
![Immunohistochemistry-Paraffin: SHP-1 Antibody (7I1K3) [NBP3-15669] - Immunohistochemistry of paraffin-embedded human appendix using SHP-1 Rabbit mAb (NBP3-15669) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SHP-1-Antibody-7I1K3-Immunohistochemistry-Paraffin-NBP3-15669-img0002.jpg)
![Immunohistochemistry-Paraffin: SHP-1 Antibody (7I1K3) [NBP3-15669] - Immunohistochemistry of paraffin-embedded mouse spleen using SHP-1 Rabbit mAb (NBP3-15669) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SHP-1-Antibody-7I1K3-Immunohistochemistry-Paraffin-NBP3-15669-img0003.jpg)
![Immunohistochemistry: SHP-1 Antibody (7I1K3) [NBP3-15669] - Immunofluorescence analysis of rat spleen using SHP-1 Rabbit mAb (NBP3-15669) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SHP-1-Antibody-7I1K3-Immunohistochemistry-NBP3-15669-img0004.jpg)
![Western Blot: PTPN6 Antibody [NBP1-83276] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/PTPN6-Antibody-Western-Blot-NBP1-83276-img0006.jpg "Western Blot: PTPN6 Antibody [NBP1-83276] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: PTPN6 Antibody [NBP1-83276] - Staining of human cell line A-431 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PTPN6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83276-img0013.jpg)
![Immunohistochemistry-Paraffin: PTPN6 Antibody [NBP1-83276] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/PTPN6-Antibody-Immunohistochemistry-Paraffin-NBP1-83276-img0012.jpg)
![Western Blot: PTPN6 Antibody [NBP1-83276] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PTPN6-Antibody-Western-Blot-NBP1-83276-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PTPN6 Antibody [NBP1-83276] - Analysis in human tonsil and skeletal muscle tissues using Anti-PTPN6 antibody. Corresponding PTPN6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PTPN6-Antibody-Immunohistochemistry-Paraffin-NBP1-83276-img0010.jpg)
![Immunohistochemistry-Paraffin: PTPN6 Antibody [NBP1-83276] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/PTPN6-Antibody-Immunohistochemistry-Paraffin-NBP1-83276-img0011.jpg)
![Western Blot: SHP-1 Antibody [NB100-58968] - Staining of Mouse Thymus lysate (A) with antibody at 0.01 ug/mL and Rat Thymus lysate with antibody at 0.1 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SHP-1-Antibody-Western-Blot-NB100-58968-img0005.jpg "Western Blot: SHP-1 Antibody [NB100-58968] - Staining of Mouse Thymus lysate (A) with antibody at 0.01 ug/mL and Rat Thymus lysate with antibody at 0.1 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: SHP-1 Antibody [NB100-58968] - Staining of paraffin embedded Human Thyroid. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/SHP-1-Antibody-Immunohistochemistry-Paraffin-NB100-58968-img0004.jpg)
![Western Blot: SHP-1 Antibody [NB100-58968] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PTPN6-Antibody-Western-Blot-NB100-58968-img0002.jpg)
![Western Blot: SHP-1 Antibody (S06-4C8) [NBP3-19611] - Western blot detection of SHP-1 in Jurkat cell lysates using NBP3-19611 (1:500 diluted). Predicted band size: 68kDa. Observed band size: 68kDa.](http://images.novusbio.com/fullsize/SHP-1-Antibody-S06-4C8-Western-Blot-NBP3-19611-img0002.jpg "Western Blot: SHP-1 Antibody (S06-4C8) [NBP3-19611] - Western blot detection of SHP-1 in Jurkat cell lysates using NBP3-19611 (1:500 diluted). Predicted band size: 68kDa. Observed band size: 68kDa.")
![Immunohistochemistry-Paraffin: SHP-1 Antibody (S06-4C8) [NBP3-19611] - Immunohistochemistry of SHP-1 in paraffin-embedded Human tonsil using NBP3-19611 at dilution 1/50](http://images.novusbio.com/fullsize/SHP-1-Antibody-S06-4C8-Immunohistochemistry-Paraffin-NBP3-19611-img0001.jpg)
![Western Blot: PTPN6 Antibody [NB100-60553] - (0.2ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PTPN6-Antibody-Western-Blot-NB100-60553-img0002.jpg "Western Blot: PTPN6 Antibody [NB100-60553] - (0.2ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: SHP-1 [p Ser591] Antibody [NBP3-23399] - Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to lambda phosphatase (lanes 2 & 4) then probed with anti-SHP-1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP-1 (Ser-591) antibody (lanes 3-6). The SHP-1 (Ser-591) antibody was used in the presence of phospho-SHP-1 (Ser-591) peptide (lane 5) or a non-specific phospho-serine peptide (lane 6).](http://images.novusbio.com/fullsize/nbp3-23399_rabbit-shp-1-p-ser591-pab-19920231911473.jpg "Western Blot: SHP-1 [p Ser591] Antibody [NBP3-23399] - Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to lambda phosphatase (lanes 2 & 4) then probed with anti-SHP-1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP-1 (Ser-591) antibody (lanes 3-6). The SHP-1 (Ser-591) antibody was used in the presence of phospho-SHP-1 (Ser-591) peptide (lane 5) or a non-specific phospho-serine peptide (lane 6).")