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![Western Blot: PIAS2 Antibody [NBP1-52022] - WB analysis of PIAS2 using anti-PIAS2 antibody on Jurkat cell lyste (30 ug cell lysate boiled in lysis buffer). Image from verified customer review.](http://images.novusbio.com/fullsize/PIAS2-Antibody-Western-Blot-NBP1-52022-img0004.jpg "Western Blot: PIAS2 Antibody [NBP1-52022] - WB analysis of PIAS2 using anti-PIAS2 antibody on Jurkat cell lyste (30 ug cell lysate boiled in lysis buffer). Image from verified customer review.")
![Western Blot: PIAS2 Antibody [NBP1-52022] - Western blot analysis of PIAS2 (aa185-196) in Human Peripheral Lymphocytes lysate (35ug protein in RIPA buffer) using NBP1-52022 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PIAS2-Antibody-Western-Blot-NBP1-52022-img0003.jpg)
![Western Blot: PIAS2 Antibody [NBP1-77159] - Rat brain tissue lysate with PIAS2 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/PIAS2-Antibody-Western-Blot-NBP1-77159-img0001.jpg "Western Blot: PIAS2 Antibody [NBP1-77159] - Rat brain tissue lysate with PIAS2 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: PIAS2 Antibody [NBP1-77159] - Rat brain tissue at 20 ug/mL.](http://images.novusbio.com/fullsize/PIAS2-Antibody-Immunofluorescence-NBP1-77159-img0006.jpg)
![Immunohistochemistry-Paraffin: PIAS2 Antibody [NBP1-77159] - Rat brain tissue with PIAS2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/PIAS2-Antibody-Immunohistochemistry-Paraffin-NBP1-77159-img0002.jpg)
![Western Blot: PIAS2 Antibody (1F7) [H00009063-M01] - Analysis of PIAS2 over-expressed 293 cell line, cotransfected with PIAS2 Validated Chimera RNAi ( Cat # H00009063-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIAS2 monoclonal antibody (M01), clone 1F7 (Cat # H00009063-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PIAS2-Antibody-1F7-Western-Blot-H00009063-M01-img0007.jpg "Western Blot: PIAS2 Antibody (1F7) [H00009063-M01] - Analysis of PIAS2 over-expressed 293 cell line, cotransfected with PIAS2 Validated Chimera RNAi ( Cat # H00009063-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIAS2 monoclonal antibody (M01), clone 1F7 (Cat # H00009063-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Immunocytochemistry/Immunofluorescence: PIAS2 Antibody (1F7) [H00009063-M01] - Analysis of monoclonal antibody to PIAS2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PIAS2-Antibody-1F7-Immunocytochemistry-Immunofluorescence-H00009063-M01-img0006.jpg)
![Proximity Ligation Assay: PIAS2 Antibody (1F7) [H00009063-M01] - Analysis of protein-protein interactions between TP53 and PIAS2. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PIAS2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PIAS2-Antibody-1F7-Proximity-Ligation-Assay-H00009063-M01-img0004.jpg)
![Sandwich ELISA: PIAS2 Antibody (1F7) [H00009063-M01] - Detection limit for recombinant GST tagged PIAS2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PIAS2-Antibody-1F7-Sandwich-ELISA-H00009063-M01-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PIAS2 Antibody [NBP2-49571] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PIAS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49571-img0007.jpg "Immunocytochemistry/Immunofluorescence: PIAS2 Antibody [NBP2-49571] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PIAS2 Antibody [NBP2-49571] - Staining in human testis and prostate tissues using anti-PIAS2 antibody. Corresponding PIAS2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PIAS2-Antibody-Immunohistochemistry-Paraffin-NBP2-49571-img0006.jpg)
![Immunohistochemistry-Paraffin: PIAS2 Antibody [NBP2-49571] - Staining of human prostate shows low expression as expected.](http://images.novusbio.com/fullsize/PIAS2-Antibody-Immunohistochemistry-Paraffin-NBP2-49571-img0004.jpg)
![Immunohistochemistry-Paraffin: PIAS2 Antibody [NBP2-49571] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/PIAS2-Antibody-Immunohistochemistry-Paraffin-NBP2-49571-img0005.jpg)
![Immunohistochemistry-Paraffin: PIAS2 Antibody [NB100-59734] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PIAS2-Antibody-Immunohistochemistry-Paraffin-NB100-59734-img0001.jpg "Immunohistochemistry-Paraffin: PIAS2 Antibody [NB100-59734] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: PIAS2 Antibody (SR1996) [NBP3-22262] - Western blot analysis using NBP3-22262 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22262_rabbit-pias2-mab-sr1996-672023106288.jpg "Western Blot: PIAS2 Antibody (SR1996) [NBP3-22262] - Western blot analysis using NBP3-22262 in HepG2 cell lysate.")
![Western Blot: PIAS2 Antibody (2B5) [NBP2-46394] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIAS2.](http://images.novusbio.com/fullsize/PIAS2-Antibody-2B5-Western-Blot-NBP2-46394-img0001.jpg "Western Blot: PIAS2 Antibody (2B5) [NBP2-46394] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIAS2.")
![Western Blot: PIAS2 Antibody (4I6K6) [NBP3-16587] - Western blot analysis of extracts of various cell lines, using PIAS2 Rabbit mAb (NBP3-16587) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PIAS2-Antibody-4I6K6-Western-Blot-NBP3-16587-img0001.jpg "Western Blot: PIAS2 Antibody (4I6K6) [NBP3-16587] - Western blot analysis of extracts of various cell lines, using PIAS2 Rabbit mAb (NBP3-16587) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: PIAS2 Overexpression Lysate (Adult Normal) [NBL1-14386] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PIAS2.](http://images.novusbio.com/fullsize/PIAS2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14386-img0002.jpg "Western Blot: PIAS2 Overexpression Lysate (Adult Normal) [NBL1-14386] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PIAS2.")
![Western Blot: PIAS2 Overexpression Lysate (Adult Normal) [NBL1-14385] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PIAS2.](http://images.novusbio.com/fullsize/PIAS2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14385-img0002.jpg "Western Blot: PIAS2 Overexpression Lysate (Adult Normal) [NBL1-14385] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PIAS2.")