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![Western Blot: PGD Antibody [NBP1-31589] - Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] diluted at 1:1000.](http://images.novusbio.com/fullsize/PGD-Antibody-Western-Blot-NBP1-31589-img0008.jpg "Western Blot: PGD Antibody [NBP1-31589] - Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PGD Antibody [NBP1-31589] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PGD protein stained by PGD antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31589-img0010.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody [NBP1-31589] - protein at cytoplasm on A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded A549 xenograft.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunohistochemistry-Paraffin-NBP1-31589-img0004.jpg)
![Western Blot: PGD Antibody [NBP1-31589] - Sample(30 ug whole cell lysate)A:293T B:MOLT4 C:Raji 10% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PGD-Antibody-Western-Blot-NBP1-31589-img0003.jpg)
![Western Blot: PGD Antibody [NBP1-31589] - Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] diluted at 1:500.](http://images.novusbio.com/fullsize/PGD-Antibody-Western-Blot-NBP1-31589-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PGD Antibody [NBP1-31589] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGD protein stained by PGD antibody [N1N3] diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31589-img0006.jpg)
![Western Blot: PGD Antibody (OTI2A5) [NBP2-45880] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGD.](http://images.novusbio.com/fullsize/PGD-Antibody-OTI2A5-Western-Blot-NBP2-45880-img0010.jpg "Western Blot: PGD Antibody (OTI2A5) [NBP2-45880] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGD.")
![Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Human thyroid tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.](http://images.novusbio.com/fullsize/PGD-Antibody-OTI2A5-Immunohistochemistry-NBP2-45880-img0009.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Human pancreas tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.](http://images.novusbio.com/fullsize/PGD-Antibody-OTI2A5-Immunohistochemistry-NBP2-45880-img0002.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Carcinoma of Human prostate tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.](http://images.novusbio.com/fullsize/PGD-Antibody-OTI2A5-Immunohistochemistry-NBP2-45880-img0003.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Human prostate tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.](http://images.novusbio.com/fullsize/PGD-Antibody-OTI2A5-Immunohistochemistry-NBP2-45880-img0004.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Human ovary tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.](http://images.novusbio.com/fullsize/PGD-Antibody-OTI2A5-Immunohistochemistry-NBP2-45880-img0005.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Human breast tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.](http://images.novusbio.com/fullsize/PGD-Antibody-OTI2A5-Immunohistochemistry-NBP2-45880-img0006.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Human Kidney tissue within the normal limits using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.](http://images.novusbio.com/fullsize/PGD-Antibody-OTI2A5-Immunohistochemistry-NBP2-45880-img0007.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody (OTI2A5) [NBP2-45880] - Human endometrium tissue using anti-PGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.](http://images.novusbio.com/fullsize/PGD-Antibody-OTI2A5-Immunohistochemistry-NBP2-45880-img0008.jpg)
![Immunofluorescence: PGD Antibody (OTI2A5) [NBP2-45880] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY PGD.](http://images.novusbio.com/fullsize/PGD-Antibody-OTI2A5-Immunofluorescence-NBP2-45880-img0001.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody [NBP2-58686] - Staining in human esophagus and skeletal muscle tissues using anti-PGD antibody. Corresponding PGD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunohistochemistry-Paraffin-NBP2-58686-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PGD Antibody [NBP2-58686] - Staining in human esophagus and skeletal muscle tissues using anti-PGD antibody. Corresponding PGD RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: PGD Antibody [NBP2-58686] - Staining of human esophagus, liver, lymph node and testis using Anti-PGD antibody NBP2-58686 (A) shows similar protein distribution across tissues to independent antibody NBP1-85925 (B).](http://images.novusbio.com/fullsize/PGD-Antibody-Immunohistochemistry-Paraffin-NBP2-58686-img0006.jpg)
![Western Blot: PGD Antibody [NBP2-58686] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.](http://images.novusbio.com/fullsize/PGD-Antibody-Western-Blot-NBP2-58686-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PGD Antibody [NBP2-58686] - Staining of human cell line A549 shows localization to cytosol & intermediate filaments.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58686-img0001.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody [NBP2-58686] - Staining of human lymph node.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunohistochemistry-Paraffin-NBP2-58686-img0008.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody [NBP2-58686] - Staining of human esophagus shows high expression.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunohistochemistry-Paraffin-NBP2-58686-img0003.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody [NBP2-58686] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunohistochemistry-Paraffin-NBP2-58686-img0004.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody [NBP2-58686] - Staining of human testis.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunohistochemistry-Paraffin-NBP2-58686-img0007.jpg)
![Western Blot: PGD Antibody [NBP1-85925] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/PGD-Antibody-Western-Blot-NBP1-85925-img0011.jpg "Western Blot: PGD Antibody [NBP1-85925] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)")
![Immunohistochemistry-Paraffin: PGD Antibody [NBP1-85925] - Staining of human lymph node.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunohistochemistry-Paraffin-NBP1-85925-img0014.jpg)
![Western Blot: PGD Antibody [NBP1-85925] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/PGD-Antibody-Western-Blot-NBP1-85925-img0005.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody [NBP1-85925] - Staining of human esophagus shows high expression.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunohistochemistry-Paraffin-NBP1-85925-img0008.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody [NBP1-85925] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunohistochemistry-Paraffin-NBP1-85925-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PGD Antibody [NBP1-85925] - Staining in human esophagus and skeletal muscle tissues using anti-PGD antibody. Corresponding PGD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunohistochemistry-Paraffin-NBP1-85925-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PGD Antibody [NBP1-85925] - Staining of human esophagus, liver, lymph node and testis using Anti-PGD antibody NBP1-85925 (A) shows similar protein distribution across tissues to independent antibody NBP2-58686 (B).](http://images.novusbio.com/fullsize/PGD-Antibody-Western-Blot-NBP1-85925-img0012.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody [NBP1-85925] - Staining of human testis.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunohistochemistry-Paraffin-NBP1-85925-img0013.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody [NBP1-85925] - Staining of human liver.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunohistochemistry-Paraffin-NBP1-85925-img0015.jpg)
![Western Blot: PGD Antibody [NBP2-19783] - Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PGD-Antibody-Western-Blot-NBP2-19783-img0004.jpg "Western Blot: PGD Antibody [NBP2-19783] - Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PGD Antibody [NBP2-19783] - Immunofluorescence analysis of methanol-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19783-img0002.jpg)
![Immunohistochemistry-Paraffin: PGD Antibody [NBP2-19783] - Paraffin-embedded human breast carcinoma. PGD antibody diluted at 1:500.](http://images.novusbio.com/fullsize/PGD-Antibody-Immunohistochemistry-Paraffin-NBP2-19783-img0005.jpg)
![Western Blot: PGD Antibody [NBP2-19783] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PGD-Antibody-Western-Blot-NBP2-19783-img0001.jpg)
![Western Blot: PGD Antibody [NBP2-19783] - Mouse tissue extracts (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody diluted at 1:500.](http://images.novusbio.com/fullsize/PGD-Antibody-Western-Blot-NBP2-19783-img0003.jpg)
![Western Blot: PGD Antibody (9A2M7) [NBP3-15277] - Western blot analysis of extracts of various cell lines, using PGD antibody (NBP3-15277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/PGD-Antibody-9A2M7-Western-Blot-NBP3-15277-img0003.jpg "Western Blot: PGD Antibody (9A2M7) [NBP3-15277] - Western blot analysis of extracts of various cell lines, using PGD antibody (NBP3-15277) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunocytochemistry/Immunofluorescence: PGD Antibody (9A2M7) [NBP3-15277] - Immunofluorescence analysis of PC-12 cells using PGD Rabbit mAb (NBP3-15277) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PGD-Antibody-9A2M7-Immunocytochemistry-Immunofluorescence-NBP3-15277-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PGD Antibody (9A2M7) [NBP3-15277] - Immunofluorescence analysis of NIH/3T3 cells using PGD Rabbit mAb (NBP3-15277) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PGD-Antibody-9A2M7-Immunocytochemistry-Immunofluorescence-NBP3-15277-img0001.jpg)
![Immunoprecipitation: PGD Antibody (9A2M7) [NBP3-15277] - Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug PGD antibody (NBP3-15277). Western blot was performed from the immunoprecipitate using PGD antibody (NBP3-15277) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/PGD-Antibody-9A2M7-Immunoprecipitation-NBP3-15277-img0004.jpg)
![Western Blot: PGD Antibody (S02-7C6) [NBP3-19520] - Western blot detection of PGD in C6, 3T3, Hela cell lysates using NBP3-19520 (1:1000 diluted). Predicted band size: 53kDa. Observed band size: 49kDa.](http://images.novusbio.com/fullsize/PGD-Antibody-S02-7C6-Western-Blot-NBP3-19520-img0002.jpg "Western Blot: PGD Antibody (S02-7C6) [NBP3-19520] - Western blot detection of PGD in C6, 3T3, Hela cell lysates using NBP3-19520 (1:1000 diluted). Predicted band size: 53kDa. Observed band size: 49kDa.")
![Immunocytochemistry/Immunofluorescence: PGD Antibody (S02-7C6) [NBP3-19520] - Immunofluorescence of PGD (green) in Hela cells using NBP3-19520 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/PGD-Antibody-S02-7C6-Immunocytochemistry-Immunofluorescence-NBP3-19520-img0001.jpg)
![Western Blot: PGD Antibody (46B3) [NBP2-59449] - Lane 1: HeLa cell lysates, Lane 2: MCF7 cell lysates, Lane 3: 293T cell lysates, Lane 4: A549 cell lysates, Lane 5: Jurkat cell lysates](http://images.novusbio.com/fullsize/PGD-Antibody-46B3-Western-Blot-NBP2-59449-img0001.jpg "Western Blot: PGD Antibody (46B3) [NBP2-59449] - Lane 1: HeLa cell lysates, Lane 2: MCF7 cell lysates, Lane 3: 293T cell lysates, Lane 4: A549 cell lysates, Lane 5: Jurkat cell lysates")
![Immunocytochemistry/Immunofluorescence: PGD Antibody (46B3) [NBP2-59449] - Analysis of PGD in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGD antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PGD-Antibody-46B3-Immunocytochemistry-Immunofluorescence-NBP2-59449-img0002.jpg)
![Western Blot: PGD Overexpression Lysate (Adult Normal) [NBL1-14329] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PGD.](http://images.novusbio.com/fullsize/PGD-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14329-img0002.jpg "Western Blot: PGD Overexpression Lysate (Adult Normal) [NBL1-14329] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PGD.")