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![Immunocytochemistry/Immunofluorescence: PEA-15 Antibody [NBP3-05544] - Western blot analysis of different tissue lysates using PEA-15 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat whole brain, [3] rat cerebellum, [4] mouse whole brain, [5] cow cortex and [6] cow cerebellum. The single strong band at about 15kDa corresponds to the PEA-15 protein.](http://images.novusbio.com/fullsize/PEA-15-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05544-img0002.jpg "Immunocytochemistry/Immunofluorescence: PEA-15 Antibody [NBP3-05544] - Western blot analysis of different tissue lysates using PEA-15 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat whole brain, [3] rat cerebellum, [4] mouse whole brain, [5] cow cortex and [6] cow cerebellum. The single strong band at about 15kDa corresponds to the PEA-15 protein.")
![Immunocytochemistry/Immunofluorescence: PEA-15 Antibody [NBP3-05544] - Immunofluorescent analysis of rat brain section stained with PEA-15 Antibody at a dilution of 1:1,000 in red, and costained with rabbit pAb to GFAP at a dilution of 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The PEA-15 antibody labels the cytoplasm of certain cells which are not labelled by the astrocyte specific GFAP antibody](http://images.novusbio.com/fullsize/PEA-15-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05544-img0001.jpg)
![Western Blot: PEA-15 Antibody [NBP3-05543] - Western blot analysis of different tissue lysates using PEA-15 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat whole brain, [3] rat cerebellum, [4] mouse whole brain, [5] cow cortex and [6] cow cerebellum. The strong band at about 15kDa corresponds to the PEA-15 protein.](http://images.novusbio.com/fullsize/PEA-15-Antibody-Western-Blot-NBP3-05543-img0002.jpg "Western Blot: PEA-15 Antibody [NBP3-05543] - Western blot analysis of different tissue lysates using PEA-15 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat whole brain, [3] rat cerebellum, [4] mouse whole brain, [5] cow cortex and [6] cow cerebellum. The strong band at about 15kDa corresponds to the PEA-15 protein.")
![Immunocytochemistry/Immunofluorescence: PEA-15 Antibody [NBP3-05543] - Immunofluorescent analysis of rat brain section stained with PEA-15 Antibody at a dilution of 1:1,000 in red, and costained with chicken pAb to MAP2 at a dilution of 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The PEA-15 antibody labels the cytoplasm of certain presumably neuronal cells which are not labelled by the astrocyte specific GFAP antibody.](http://images.novusbio.com/fullsize/PEA-15-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05543-img0001.jpg)
![Western Blot: PEA-15 Antibody [NBP2-88031] - WB Suggested Anti-PEA15 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain](http://images.novusbio.com/fullsize/PEA-15-Antibody-Western-Blot-NBP2-88031-img0002.jpg "Western Blot: PEA-15 Antibody [NBP2-88031] - WB Suggested Anti-PEA15 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain")
![Immunohistochemistry-Paraffin: PEA-15 Antibody [NBP2-88031] - Rabbit Anti-PEA15 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue. Observed Staining: Cytoplasmic. Primary Antibody Concentration: 1:100.](http://images.novusbio.com/fullsize/PEA-15-Antibody-Immunohistochemistry-Paraffin-NBP2-88031-img0001.jpg)
![Western Blot: PEA-15 Antibody [NBP2-88031] - WB Suggested Anti-PEA15 Antibody. Positive Control: Lane 1: 20ug EGFP-transfected MCF7 lysate Lane 2: 20ug EGFP-PEA15 transfected MCF7 lysate. Primary Antibody Dilution : 1:1000. Secondary Antibody : Goat anti-rabbit-HRP. Secondry Antibody Dilution : 1:50](http://images.novusbio.com/fullsize/PEA-15-Antibody-Western-Blot-NBP2-88031-img0003.jpg)
![Immunohistochemistry-Paraffin: PEA-15 Antibody [NBP2-62670] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/PEA-15-Antibody-Immunohistochemistry-Paraffin-NBP2-62670-img0002.jpg "Immunohistochemistry-Paraffin: PEA-15 Antibody [NBP2-62670] - Staining of human cerebral cortex shows high expression.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PEA-15 Antibody [NBP2-62670] - Analysis in human cerebral cortex and colon tissues using Anti-PEA15 antibody. Corresponding PEA15 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PEA-15-Antibody-Immunohistochemistry-Paraffin-NBP2-62670-img0001.jpg)
![Immunohistochemistry-Paraffin: PEA-15 Antibody [NBP2-62670] - Staining of human colon shows low expression as expected.](http://images.novusbio.com/fullsize/PEA-15-Antibody-Immunohistochemistry-Paraffin-NBP2-62670-img0003.jpg)
![Western Blot: PEA-15 Antibody [NBP2-93741] - Analysis of extracts of various cell lines, using PEA-15 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/PEA-15-Antibody-Western-Blot-NBP2-93741-img0001.jpg "Western Blot: PEA-15 Antibody [NBP2-93741] - Analysis of extracts of various cell lines, using PEA-15 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: PEA-15 Antibody [NBP1-81732] - Staining of human cell line U-2 OS shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PEA-15-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81732-img0001.jpg "Immunocytochemistry/Immunofluorescence: PEA-15 Antibody [NBP1-81732] - Staining of human cell line U-2 OS shows localization to nucleus & cytosol. Antibody staining is shown in green.")
![Western Blot: PEA15 Overexpression Lysate (Adult Normal) [NBL1-14273] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PEA15.](http://images.novusbio.com/fullsize/PEA15-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14273-img0002.jpg "Western Blot: PEA15 Overexpression Lysate (Adult Normal) [NBL1-14273] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PEA15.")
![SDS-Page: PEA15 Protein [NBP1-30247] - PEA-15, 15 kDa (130aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE](http://images.novusbio.com/fullsize/PEA15-Protein-SDS-Page-NBP1-30247-img0001.jpg "SDS-Page: PEA15 Protein [NBP1-30247] - PEA-15, 15 kDa (130aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE")