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52 Results for "p70s6k"
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AF8962

p70 S6 Kinase/S6K Antibody [Unconjugated]

(6 Publications)
100 μg
AF8962
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human p70 S6 Kinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8962) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for p70 S6 Kinase at approximately 70 and 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, IHC, CyTOF-ready, Flow
Applications: WB, Simple Western, IHC, CyTOF-ready, Flow
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF8962-SP
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MAB8963

p70 S6 Kinase/S6K [p Thr389] Antibody (1045C) [Unconjugated]

(6 Publications)
100 μg
MAB8963
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 60 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-p70 S6 Kinase (T389) Monoclonal Antibody (Catalog # MAB8963) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T389) and Phospho-p85 S6 Kinase (T412) at approximately 70 and 85 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, ICC/IF
Applications: WB, ICC/IF
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB8963-SP
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AF8965

p70 S6 Kinase/S6K [p Thr421, p Ser424] Antibody [Unconjugated]

(6 Publications)
100 μg
AF8965
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL of Recombinant Human IGF-1 (Catalog # 291-G1) for 20 minutes and NIH-3T3 mouse embryonic fibroblast cell line untreated or treated with 100 ng/mL of Human PDGF (Catalog # 120-HD) for 20 minutes. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-p70 S6 Kinase (T421/S424) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8965), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T421/S424) at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western
Applications: WB, Simple Western
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF8965-SP
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MAB8962

p70 S6 Kinase/S6K Antibody (215247) [Unconjugated]

(3 Publications)
100 μg
MAB8962
HeLa human cervical epithelial carcinoma cell line was stained with Human/Mouse/Rat p70 S6 Kinase Monoclonal Antibody (Catalog # MAB8962, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, IHC, CyTOF-ready, Flow
Applications: WB, IHC, CyTOF-ready, Flow
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 IC8962U-100UG
Alexa Fluor 405 IC8962V-100UG
Alexa Fluor 488 IC8962G-025
Alexa Fluor 532 FAB8962X
Alexa Fluor 594 IC8962T-100UG
Alexa Fluor 647 IC8962R-100UG
Alexa Fluor 700 IC8962N-100UG
Alexa Fluor 750 IC8962S-100UG
Allophycocyanin FAB8962A
Biotin FAB8962B
DyLight 350 FAB8962D
DyLight 405 FAB8962E
DyLight 488 FAB8962K
DyLight 550 FAB8962L
DyLight 594 FAB8962M
DyLight 650 FAB8962W
DyLight 680 FAB8962Y
DyLight 755 FAB8962Z
FITC FAB8962F
HRP FAB8962H
Janelia Fluor 549 FAB8962I
Janelia Fluor 646 FAB8962J
PerCP FAB8962C
Phycoerythrin IC8962P-025
mFluor Violet 450 SE FAB8962MFV450
mFluor Violet 500 SE FAB8962MFV500
mFluor Violet 610 SE FAB8962MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB8962-SP
Carrier Free IC8962P-025
Carrier Free IC8962G-025
Carrier Free IC8962V-100UG
Carrier Free IC8962R-100UG
Carrier Free IC8962N-100UG
Carrier Free IC8962U-100UG
Carrier Free IC8962T-100UG
Carrier Free IC8962S-100UG
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H00006198-M04

p70 S6 Kinase/S6K Antibody (4H4)

(1 Review)
0.1 mg
H00006198-M04
Western Blot: p70 S6 Kinase/S6K Antibody (4H4) [H00006198-M04] - Analysis of RPS6KB1 expression in NIH/3T3 (Cat # L018V1).
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, ELISA, ICC/IF
Applications: WB, ELISA, ICC/IF
Mouse Monoclonal
Host: Mouse Monoclonal
Unconjugated  
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NBP2-00754

p70 S6 Kinase/S6K Antibody (OTI1G4)

0.1 ml
NBP2-00754
Western Blot: S6K Antibody (1G4) [NBP2-00754] Analysis of extracts (35ug) from 9 different cell lines by using anti-S6K monoclonal antibody.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Flow, ICC/IF, IHC
Applications: WB, Flow, ICC/IF, IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 NBP2-73209AF350
Alexa Fluor 405 NBP2-73209AF405
Alexa Fluor 488 NBP2-73209AF488
Alexa Fluor 532 NBP2-73209AF532
Alexa Fluor 594 NBP2-73209AF594
Alexa Fluor 647 NBP2-73209AF647
Alexa Fluor 700 NBP2-73209AF700
Alexa Fluor 750 NBP2-73209AF750
Allophycocyanin NBP2-73209APC
Allophycocyanin/Cy7 NBP2-73209APCCY7
Biotin NBP2-73209B
CoraFluor 1 NBP2-73209CL1
DyLight 350 NBP2-73209UV
DyLight 405 NBP2-73209V
DyLight 488 NBP2-73209G
DyLight 550 NBP2-73209R
DyLight 594 NBP2-73209DL594
DyLight 650 NBP2-73209C
DyLight 680 NBP2-73209FR
DyLight 755 NBP2-73209IR
FITC NBP2-73209F
HRP NBP2-73209H
Janelia Fluor 525 NBP2-73209JF525
Janelia Fluor 549 NBP2-73209JF549
Janelia Fluor 585 NBP2-73209JF585
Janelia Fluor 635 NBP2-73209JF635
Janelia Fluor 646 NBP2-73209JF646
Janelia Fluor 669 NBP2-73209JF669
PE NBP2-73209PE
PE/Atto594 NBP2-73209PEATT594
PE/Cy5.5 NBP2-73209PECY55
PE/Cy7 NBP2-73209PECY7
PerCP NBP2-73209PCP
mFluor Violet 450 SE NBP2-73209MFV450
mFluor Violet 500 SE NBP2-73209MFV500
mFluor Violet 610 SE NBP2-73209MFV610
Available Formulations
Formulation Catalog # Availability Price  
Azide and BSA Free NBP2-73209
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NBP2-23649

p70 S6 Kinase/S6K Antibody

(1 Publication)
100 ul
NBP2-23649
Western Blot: p70 S6 Kinase/S6K Antibody [NBP2-23649] - Western blot Image of anti-p70 S6 Kinase. Whole cell protein from HeLa (lane 1), HEK293 (lane 2) and MCF7 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-p70 S6 Kinase in 1% milk, and detected with an anti-goat HRP secondary antibody using chemiluminescence.
Hu, Mu, Rt, Bv, Rb
Species Glossary
Reactivity: Hu, Mu, Rt, Bv, Rb
WB, ELISA, ICC/IF (-)
Applications: WB, ELISA, ICC/IF (-)
Goat Polyclonal
Host: Goat Polyclonal
IMG-6213A
Alt Catalog# IMG-6213A
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
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NBP3-16752

p70 S6 Kinase beta/S6K2 Antibody (5D8F5)

100 ul
NBP3-16752
Western Blot: p70 S6 Kinase beta/S6K2 Antibody (5D8F5) [NBP3-16752] - Western blot analysis of extracts of Mouse brain, using p70 S6 Kinase beta/S6K2 Rabbit mAb (NBP3-16752) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, ICC/IF, IHC
Applications: WB, ICC/IF, IHC
Rabbit Monoclonal
Host: Rabbit Monoclonal
Unconjugated  
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896-KS

Recombinant Human Active p70 S6 Kinase Protein, CF

(4 Publications)
10 μg
896-KS
The approximate molecular weight is 76 kDa and the average purity is 73%.
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
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AF8964

p70 S6 Kinase/S6K Antibody [Unconjugated]

(1 Publication)
100 μg
AF8964
Western blot shows lysates of MCF-7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat p70 S6 Kinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8964) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for p70 S6 Kinase at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, IHC
Applications: WB, IHC
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF8964-SP
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MAB8964

p70 S6 Kinase/S6K [p Thr229] Antibody (1015B) [Unconjugated]

(2 Publications)
100 μg
MAB8964
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 1 hour. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-p70 S6 Kinase (T229) Monoclonal Antibody (Catalog # MAB8964) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T229) at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, ICC/IF
Applications: WB, ICC/IF
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB8964-SP
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NB100-293

p70 S6 Kinase/S6K Antibody

(6 Publications)
100 ul
NB100-293
Western Blot: p70 S6 Kinase/S6K Antibody [NB100-293] - Detection of Human and Mouse p70S6K by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p70S6K antibody NB100-293 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Hu, Mu, Rt, Bv, Rb
Species Glossary
Reactivity: Hu, Mu, Rt, Bv, Rb
WB, Simple Western
Applications: WB, Simple Western
Rabbit Polyclonal
Host: Rabbit Polyclonal
Unconjugated  
Available Conjugates:
         
Validated by:          
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 NB100-293AF350
Alexa Fluor 405 NB100-293AF405
Alexa Fluor 488 NB100-293AF488
Alexa Fluor 532 NB100-293AF532
Alexa Fluor 594 NB100-293AF594
Alexa Fluor 647 NB100-293AF647
Alexa Fluor 700 NB100-293AF700
Alexa Fluor 750 NB100-293AF750
Allophycocyanin NB100-293APC
Biotin NB100-293B
CoraFluor 1 NB100-293CL1
DyLight 350 NB100-293UV
DyLight 405 NB100-293V
DyLight 488 NB100-293G
DyLight 550 NB100-293R
DyLight 594 NB100-293DL594
DyLight 650 NB100-293C
DyLight 680 NB100-293FR
DyLight 755 NB100-293IR
FITC NB100-293F
HRP NB100-293H
Janelia Fluor 525 NB100-293JF525
Janelia Fluor 549 NB100-293JF549
Janelia Fluor 585 NB100-293JF585
Janelia Fluor 635 NB100-293JF635
Janelia Fluor 646 NB100-293JF646
Janelia Fluor 669 NB100-293JF669
PE NB100-293PE
PerCP NB100-293PCP
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