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![Western Blot: MDH2 Antibody [NBP1-32259] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-32259-img0017.jpg "Western Blot: MDH2 Antibody [NBP1-32259] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: MDH2 Antibody [NBP1-32259] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MDH2 stained by MDH2 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10um.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32259-img0023.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Rat colon. MDH2 stained by MDH2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0021.jpg)
![Western Blot: MDH2 Antibody [NBP1-32259] - Analysis of MDH2 in HeLa whole cell lysate. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-32259-img0007.jpg)
![Western Blot: MDH2 Antibody [NBP1-32259] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-32259-img0009.jpg)
![Western Blot: MDH2 Antibody [NBP1-32259] - A. 50 ug rat lung lysate/extract 10 % SDS-PAGEMDH2 antibody dilution: 1:5000](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-32259-img0012.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - H1299 xenograft. MDH2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0006.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Mouse heart. MDH2 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0013.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Mouse heart. MDH2 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0014.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Rat liver. MDH2 stained by MDH2 antibody diluted at 1:1000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0018.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Rat testis. MDH2 stained by MDH2 antibody diluted at 1:1000. Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0019.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Mouse testis. MDH2 stained by MDH2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-32259-img0020.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: MDH2 Antibody [NBP1-32259] - Wild-type (WT) and MDH2 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MDH2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-32259-img0022.jpg)
![Western Blot: MDH2 Antibody [NBP1-86036] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-86036-img0015.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MDH2 Antibody [NBP1-86036] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MDH2 Antibody [NBP1-86036] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86036-img0008.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-86036] - Staining of human colon.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-86036-img0014.jpg)
![Western Blot: MDH2 Antibody [NBP1-86036] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-86036-img0007.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-86036] - Staining of human lymph node.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-86036-img0011.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-86036] - Staining of human liver.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-86036-img0012.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-86036] - Staining of human kidney.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-86036-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-86036] - Staining of human colon, kidney, liver and lymph node using Anti-MDH2 antibody NBP1-86036 (A) shows similar protein distribution across tissues to independent antibody NBP1-89540 (B).](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-86036-img0009.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89518] - Staining of human colon, kidney, liver and lymph node using Anti-MDH2 antibody NBP1-89518 (A) shows similar protein distribution across tissues to independent antibody NBP1-86036 (B).](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89518-img0008.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89518] - Staining of human colon, kidney, liver and lymph node using Anti-MDH2 antibody NBP1-89518 (A) shows similar protein distribution across tissues to independent antibody NBP1-86036 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: MDH2 Antibody [NBP1-89518] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-89518-img0014.jpg)
![Western Blot: MDH2 Antibody [NBP1-89518] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-89518-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: MDH2 Antibody [NBP1-89518] - Staining of human cell line U-251 MG shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89518-img0007.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89518] - Staining of human colon.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89518-img0013.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89518] - Staining of human lymph node.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89518-img0010.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89518] - Staining of human liver.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89518-img0011.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89518] - Staining of human kidney.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89518-img0012.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human colon, kidney, liver and lymph node using Anti-MDH2 antibody NBP1-89540 (A) shows similar protein distribution across tissues to independent antibody NBP1-86036 (B).](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89540-img0015.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human colon, kidney, liver and lymph node using Anti-MDH2 antibody NBP1-89540 (A) shows similar protein distribution across tissues to independent antibody NBP1-86036 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: MDH2 Antibody [NBP1-89540] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-89540-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: MDH2 Antibody [NBP1-89540] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining shown in green.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89540-img0010.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89540-img0024.jpg)
![Western Blot: MDH2 Antibody [NBP1-89540] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-89540-img0009.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human colon.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89540-img0017.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human lymph node.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89540-img0018.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human kidney.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89540-img0019.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human pancreas shows moderate to strong granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89540-img0021.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human skeletal muscle shows moderate to strong granular cytoplasmic positivity in myocytes](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89540-img0022.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89540] - Staining of human liver shows moderate to strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89540-img0023.jpg)
![Western Blot: MDH2 Antibody [NBP1-89539] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-89539-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MDH2 Antibody [NBP1-89539] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1,. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89539] - Staining of human colon.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89539-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89539] - Staining of human colon, kidney, liver and lymph node using Anti-MDH2 antibody NBP1-89539 (A) shows similar protein distribution across tissues to independent antibody NBP1-86036 (B).](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89539-img0007.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89539] - Staining of human lymph node.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89539-img0009.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89539] - Staining of human kidney.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89539-img0010.jpg)
![Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-89539] - Staining of human liver.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-Paraffin-NBP1-89539-img0011.jpg)
![Western Blot: MDH2 Antibody (S06-4E2) [NBP3-19685] - Western blot detection of MDH2 in K562, Rat Brain, Hela cell lysates using NBP3-19685 (1:1000 diluted). Predicted band size: 36kDa. Observed band size: 36kDa.](http://images.novusbio.com/fullsize/MDH2-Antibody-S06-4E2-Western-Blot-NBP3-19685-img0002.jpg "Western Blot: MDH2 Antibody (S06-4E2) [NBP3-19685] - Western blot detection of MDH2 in K562, Rat Brain, Hela cell lysates using NBP3-19685 (1:1000 diluted). Predicted band size: 36kDa. Observed band size: 36kDa.")
![Immunohistochemistry-Paraffin: MDH2 Antibody (S06-4E2) [NBP3-19685] - Immunohistochemistry of MDH2 in paraffin-embedded Human breast cancer tissue using NBP3-19685 at dilution 1/20](http://images.novusbio.com/fullsize/MDH2-Antibody-S06-4E2-Immunohistochemistry-Paraffin-NBP3-19685-img0001.jpg)
![Western Blot: MDH2 Antibody (S05-4B5) [NBP3-19686] - Western blot detection of MDH2 in C6, 3T3, Hela cell lysates using NBP3-19686 (1:1000 diluted). Predicted band size: 36kDa. Observed band size: 36kDa.](http://images.novusbio.com/fullsize/MDH2-Antibody-S05-4B5-Western-Blot-NBP3-19686-img0002.jpg "Western Blot: MDH2 Antibody (S05-4B5) [NBP3-19686] - Western blot detection of MDH2 in C6, 3T3, Hela cell lysates using NBP3-19686 (1:1000 diluted). Predicted band size: 36kDa. Observed band size: 36kDa.")
![Immunohistochemistry-Paraffin: MDH2 Antibody (S05-4B5) [NBP3-19686] - Immunohistochemistry of MDH2 in paraffin-embedded Human breast cancer tissue using NBP3-19686 at dilution 1/50](http://images.novusbio.com/fullsize/MDH2-Antibody-S05-4B5-Immunohistochemistry-Paraffin-NBP3-19686-img0001.jpg)
![Western Blot: MDH2 Antibody (JE64-68) [NBP3-32583] - Western blot analysis of MDH2 on different lysates with Rabbit anti-MDH2 antibody (NBP3-32583) at 1/500 dilution. Lane 1: Rat lung tissue lysate (20 ug/Lane) Lane 2: Hela cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32583) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32583_rabbit-mdh2-mab-je64-68-278202415242618.jpg "Western Blot: MDH2 Antibody (JE64-68) [NBP3-32583] - Western blot analysis of MDH2 on different lysates with Rabbit anti-MDH2 antibody (NBP3-32583) at 1/500 dilution. Lane 1: Rat lung tissue lysate (20 ug/Lane) Lane 2: Hela cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32583) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MDH2 Antibody (JE64-68) [NBP3-32583] - Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-MDH2 antibody (NBP3-32583) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32583) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32583_rabbit-mdh2-mab-je64-68-298202413585596.jpg)
![ELISA: MDH2 Antibody (2A7) [H00004191-M06] - Detection limit for recombinant GST tagged MDH2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MDH2-Antibody-2A7-ELISA-H00004191-M06-img0001.jpg "ELISA: MDH2 Antibody (2A7) [H00004191-M06] - Detection limit for recombinant GST tagged MDH2 is approximately 1ng/ml as a capture antibody.")
![Western Blot: MDH2 Antibody [NBP1-54649] - This Anti-MDH2 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 2.5ug/ml.](http://images.novusbio.com/fullsize/MDH2-Antibody-Western-Blot-NBP1-54649-img0001.jpg "Western Blot: MDH2 Antibody [NBP1-54649] - This Anti-MDH2 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 2.5ug/ml.")
![Western Blot: MDH2 Antibody [NBP1-54649] - C-terminal region staining of Proximal kidney tubules purfied from cortex at 1:1000.](http://images.novusbio.com/fullsize/MDH2-Antibody-Immunohistochemistry-NBP1-54649-img0002.jpg)
![Western Blot: MDH2 Antibody (1G12) [H00004191-M01] - MDH2 monoclonal antibody (M01), clone 1G12. Analysis of MDH2 expression in IMR-32.](http://images.novusbio.com/fullsize/MDH2-Antibody-1G12-Western-Blot-H00004191-M01-img0002.jpg "Western Blot: MDH2 Antibody (1G12) [H00004191-M01] - MDH2 monoclonal antibody (M01), clone 1G12. Analysis of MDH2 expression in IMR-32.")
![Sandwich ELISA: MDH2 Antibody (1G12) [H00004191-M01] - Detection limit for recombinant GST tagged MDH2 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MDH2-Antibody-1G12-Sandwich-ELISA-H00004191-M01-img0001.jpg)
![SDS-Page: Recombinant Human MDH2 Protein [NBP1-48325] - 15% SDS-PAGE (3 ug).](http://images.novusbio.com/fullsize/Recombinant-Human-MDH2-Protein-SDS-Page-NBP1-48325-img0002.jpg "SDS-Page: Recombinant Human MDH2 Protein [NBP1-48325] - 15% SDS-PAGE (3 ug).")