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![Western Blot: Sin1/MAPKAP1 Antibody [NB110-40424] - Truncated Sin1/MAPKAP1 displaces endogenous Sin1/MAPKAP1 from mTORC2 in DLD1 colon cancer cells. Sin1/MAPKAP1 constructs incorporate into mTORC2 and displace endogenous Sin1/MAPKAP1. Constructs were induced for 72 hours prior to immune precipitation. mTORC2 subunits, mTOR and Rictor, only appear in myc immunoprecipitates after induction with doxycycline (Left panels); myc-deltaSin1/MAPKAP1 cannot be directly detected in precipitates due to secondary antibody cross reaction with precipitating IgG. Right panels indicate unchanging expression levels of Rictor and mTOR in immune precipitation input lysates, which is further quantified from 3 independent experiments. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/20086), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Western-Blot-NB110-40424-img0010.jpg "Western Blot: Sin1/MAPKAP1 Antibody [NB110-40424] - Truncated Sin1/MAPKAP1 displaces endogenous Sin1/MAPKAP1 from mTORC2 in DLD1 colon cancer cells. Sin1/MAPKAP1 constructs incorporate into mTORC2 and displace endogenous Sin1/MAPKAP1. Constructs were induced for 72 hours prior to immune precipitation. mTORC2 subunits, mTOR and Rictor, only appear in myc immunoprecipitates after induction with doxycycline (Left panels); myc-deltaSin1/MAPKAP1 cannot be directly detected in precipitates due to secondary antibody cross reaction with precipitating IgG. Right panels indicate unchanging expression levels of Rictor and mTOR in immune precipitation input lysates, which is further quantified from 3 independent experiments. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/20086), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NB110-40424] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- Sin1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Immunohistochemistry-Paraffin-NB110-40424-img0009.jpg)
![Western Blot: Sin1/MAPKAP1 Antibody [NB110-40424] - Detection of Human Sin1 on HeLa whole cell lysate.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Western-Blot-NB110-40424-img0008.jpg)
![Western Blot: Sin1/MAPKAP1 Antibody [NBP2-24523] - Analysis of SIN1 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse skeletal muscle lysate and 4) rat skeletal muscle lysate using SIN1 at 2 ug/ml.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Western-Blot-NBP2-24523-img0006.jpg "Western Blot: Sin1/MAPKAP1 Antibody [NBP2-24523] - Analysis of SIN1 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse skeletal muscle lysate and 4) rat skeletal muscle lysate using SIN1 at 2 ug/ml.")
![Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NBP2-24523] - Analysis of Transportin 1 in human liver tissue using Transportin 1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-24523-img0005.jpg)
![Western Blot: Sin1/MAPKAP1 Antibody [NB100-56482] - Analysis of Mip1 in human heart lysate in the 1) absence and 2) presence of immunizing peptide and in human skeletal muscle lysate in the 3) absence and 4) presence of immunizing peptide using this antibody. 5 ug/ml.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Western-Blot-NB100-56482-img0003.jpg "Western Blot: Sin1/MAPKAP1 Antibody [NB100-56482] - Analysis of Mip1 in human heart lysate in the 1) absence and 2) presence of immunizing peptide and in human skeletal muscle lysate in the 3) absence and 4) presence of immunizing peptide using this antibody. 5 ug/ml.")
![Western Blot: Sin1/MAPKAP1 Antibody [NBP1-89569] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Western-Blot-NBP1-89569-img0004.jpg "Western Blot: Sin1/MAPKAP1 Antibody [NBP1-89569] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NBP1-89569] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89569-img0008.jpg)
![Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NBP1-89569] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89569-img0006.jpg)
![Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NBP1-89569] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89569-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NBP1-89569] - Staining in human testis and pancreas tissues using anti-MAPKAP1 antibody. Corresponding MAPKAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89569-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Sin1/MAPKAP1 Antibody [NBP1-89568] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89568-img0006.jpg "Immunocytochemistry/Immunofluorescence: Sin1/MAPKAP1 Antibody [NBP1-89568] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry-Paraffin: Sin1/MAPKAP1 Antibody [NBP1-89568] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-89568-img0005.jpg)
![Western Blot: SIN1 Overexpression Lysate (Adult Normal) [NBP2-10750] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIN1.](http://images.novusbio.com/fullsize/SIN1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10750-img0001.jpg "Western Blot: SIN1 Overexpression Lysate (Adult Normal) [NBP2-10750] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIN1.")
![Western Blot: SIN1 Lysate [NBL1-12887] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for MAPKAP1](http://images.novusbio.com/fullsize/SIN1-Lysate-Western-Blot-NBL1-12887-img0001.jpg "Western Blot: SIN1 Lysate [NBL1-12887] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for MAPKAP1")
![Western Blot: SIN1 Overexpression Lysate (Adult Normal) [NBP2-06349] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIN1.](http://images.novusbio.com/fullsize/SIN1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06349-img0001.jpg "Western Blot: SIN1 Overexpression Lysate (Adult Normal) [NBP2-06349] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIN1.")