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Sin1/MAPKAP1 Antibody (752219)

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Western blot shows lysates of human heart tissue and human kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Sin1/MAPKAP1 Monoclonal Antibody (Catalog # MAB8168) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
752219
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Sin1/MAPKAP1 Antibody (752219) Summary

Immunogen
E. coli-derived recombinant human Sin1/MAPKAP1
Lys408-Gln522
Accession # Q9BPZ7
Specificity
Detects human Sin1/MAPKAP1 in ELISA.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MAPKAP1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB8168 in the following application:

Publications
Read Publication using MAB8168.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sin1/MAPKAP1 Antibody (752219)

  • JC310
  • MAPKAP1
  • MEKK2-interacting protein 1
  • MGC2745
  • MIP1
  • MIP1SIN1SIN1b
  • Mitogen-activated protein kinase 2-associated protein 1
  • mitogen-activated protein kinase associated protein 1
  • mSIN1
  • ras inhibitor MGC2745
  • SAPK-interacting protein 1
  • Sin1
  • SIN1g
  • stress-activated map kinase interacting protein 1
  • Stress-activated map kinase-interacting protein 1
  • stress-activated protein kinase-interacting 1
  • target of rapamycin complex 2 subunit MAPKAP1
  • TORC2 subunit MAPKAP1

Background

MAPKAP1 (mitogen-activated protein kinase (MAPK) associated protein-1), also called Mip1 (MAPK interacting protein 1) or Sin1 (stress-activated protein kinase (SAPK) interacting 1), is a widely expressed 59-65 kDa cytoplasmic phosphoprotein. MAPKAP1 binds to inactive forms of signaling molecules such as MEKK2 and Ras. Alternate mRNA splicing potentially creates isoforms of 323, 330, 372, 475, 486 and 522 amino acids (aa) in humans. The human MAPKAP1 region used as an immunogen is included in all except the smallest isoform, and shares 96% aa sequence identity with mouse and rat MAPKAP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Sin1/MAPKAP1 Antibody (MAB8168)(1)


Review for Sin1/MAPKAP1 Antibody (MAB8168) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB8168:
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(1)
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Images Ratings Applications Species Date Details
Western Blot Sin1/MAPKAP1 MAB8168
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4
reviewed by:
Verified Customer
WB Mouse 10/23/2019
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Summary

ApplicationWestern Blot
Sample TestedAdipose tissue
SpeciesMouse
LotCGFJ011909A

Comments

CommentsMouse white adipose tissue was homogenised and protein content was quantified by a BCA assay. Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membranes. Membranes were probed with primary antibody MAPKAP1 diluted 1:1000 in 5% BSA 2, before incubation with anti mouse secondary horseradish peroxidase-conjugated antibody 1:5000. Blots were visualised with Immobilon Western Chemiluminescence HRP Substrate and imaged with Syngene chemiluminescence imaging system.

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Verified Customer
10/23/2019
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MAPKAP1
Uniprot