| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1819-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76511-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1819-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5334-SP | |||
| Carrier Free | MAB5334-500 | |||
![N/A Klotho [Biotin]](http://images.novusbio.com/fullsize2/DATA_Klotho_DY533405_ELISA_2477.jpg "N/A Klotho [Biotin]")
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human KLOTHO recombinant protein per lane. Antibodies: KLOTHO NBP1-76511 (Lane 1: 1 ug/mL, Lane 2: 2 ug/mL and Lane 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Western-Blot-NBP1-76511-img0010.jpg "Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human KLOTHO recombinant protein per lane. Antibodies: KLOTHO NBP1-76511 (Lane 1: 1 ug/mL, Lane 2: 2 ug/mL and Lane 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] - Mouse heart tissue diluted at 20 ug/ml.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-76511-img0002.jpg)
![Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Endothelial klotho staining. Image collected and cropped by CiteAb from the following publication (cardiab.biomedcentral.com/articles/10.1186/s12933-016-0414-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Immunohistochemistry-NBP1-76511-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - ARPE-19 cells were divided into the following groups: normal, hypoxia, hypoxia + AAV vector, hypoxia + digoxin, hypoxia + digoxin + nutlin-3a, hypoxia + AAV-p53 mutant infection and hypoxia + miRNA-34a inhibitor. Western blot assay of Klotho in ARPE-19 cells was performed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33438362/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Klotho Antibody - BSA Free-Western Blot-NBP1-76511-img0011.jpg)
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Validation in Rat Kidney Tissue LysateLoading: 15ug of lysates per lane.Antibodies: KLOTHO 6107 (1ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Western-Blot-NBP1-76511-img0007.jpg)
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: KLOTHO NBP1-76511 (2 ug/mL), KLOTHO, competitor antibody (4 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Western-Blot-NBP1-76511-img0009.jpg)
![Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] - Mouse heart tissue diluted at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-76511-img0003.jpg)
![Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] - Staining of rat heart tissue with antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Klotho-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-76511-img0004.jpg)
![Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Blockade of the HIF‐1 alpha /p53/miRNA‐34a/Klotho axis decreases the leakage & area of mouse laser‐induced CNV. The mice were divided into the following groups: normal, CNV 7 d, CNV 7 d + 0.1% DMSO, CNV 7 d + digoxin (oral; 2 mg/kg for 7 d), CNV 7 d + AAV‐p53 mutant (intravitreal injection; approximately 3 μL, 3 × 1010 viral particles/mL), CNV 7 d + miRNA‐34a inhibitor (intravitreal injection; 1 μg) & CNV 7 d + AAV‐Klotho full‐length plasmid (intravitreal injection, 2 μL, 5 × 1010 viral particles/mL). A, Western blot was performed to measure HIF‐1 alpha , p‐p53 (S15), p53 (S20), p‐p53 (S46), p53 & Klotho protein levels. B, The relative protein levels of HIF‐1 alpha /GAPDH (B), p‐p53 (S15)/p53 (C), p‐p53 (S20)/p53 (D), p‐p53 (S46)/p53 (E), p53/GAPDH (F) & Klotho/GAPDH (G) were analysed. ***P Figure 4 Immunohistochemistry Validation of Klotho in Mouse Heart Tissue Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-2711202419353832.jpg)
![Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Figure 4 Immunohistochemistry Validation of Klotho in Mouse Heart Tissue Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-2711202419562112.jpg)
![Immunocytochemistry/ Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] - Figure 5 Immunofluorescence Validation of Klotho in Mouse Heart TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling Klotho with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-2711202419353822.jpg)
![Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Figure 6 Immunohistochemistry Validation of Klotho in Rat Heart Tissue Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-2711202420272449.gif)
![Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511] - Figure 6 Immunohistochemistry Validation of Klotho in Rat Heart Tissue Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Klotho antibody at 2.5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-271120242013162.gif)
![Immunocytochemistry/ Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] - Figure 5 Immunofluorescence Validation of Klotho in Mouse Heart TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling Klotho with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76511_rabbit-polyclonal-klotho-antibody-2711202420212384.jpg)
![Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432] - Staining of Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Klotho-Antibody-Immunohistochemistry-Paraffin-NB100-2432-img0007.jpg "Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432] - Staining of Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.")
![Immunohistochemistry-Paraffin: Klotho Antibody [NB100-2432] - Staining of Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/Klotho-Antibody-Immunohistochemistry-Paraffin-NB100-2432-img0005.jpg)
![Western Blot: Klotho Antibody [NBP1-55097] - Titration: 0.2-1 ug/ml, Positive Control: Human Thymus.](http://images.novusbio.com/fullsize/Klotho-Antibody-Western-Blot-NBP1-55097-img0003.jpg "Western Blot: Klotho Antibody [NBP1-55097] - Titration: 0.2-1 ug/ml, Positive Control: Human Thymus.")
![ELISA: Mouse Klotho ELISA Kit (Colorimetric) [NBP3-18030] - Samples were spiked with high concentrations of Mouse Klotho and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-18030_mouse-klotho-elisa-kit-colorimetric-13220241612139.jpg "ELISA: Mouse Klotho ELISA Kit (Colorimetric) [NBP3-18030] - Samples were spiked with high concentrations of Mouse Klotho and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Klotho ELISA Kit (Colorimetric) [NBP3-18030] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-18030_mouse-klotho-elisa-kit-colorimetric-432024924058.jpg)
![Western Blot: Klotho Overexpression Lysate (Adult Normal) [NBL1-12307] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Klotho.](http://images.novusbio.com/fullsize/Klotho-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12307-img0002.jpg "Western Blot: Klotho Overexpression Lysate (Adult Normal) [NBL1-12307] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Klotho.")