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![Western Blot: Islet-1 Antibody [NBP2-14999] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Islet-1-Antibody-Western-Blot-NBP2-14999-img0008.jpg "Western Blot: Islet-1 Antibody [NBP2-14999] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Islet-1 Antibody [NBP2-14999] - SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Islet 1 protein stained by Islet 1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Islet-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14999-img0003.jpg)
![Immunohistochemistry-Frozen: Islet-1 Antibody [NBP2-14999] - Frozen sectioned adult mouse retina. Green: Islet 1 protein stained by Islet 1 antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Islet-1-Antibody-Immunohistochemistry-Frozen-NBP2-14999-img0004.jpg)
![Western Blot: Islet-1 Antibody [NBP2-14999] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Islet-1-Antibody-Western-Blot-NBP2-14999-img0002.jpg)
![Western Blot: Islet-1 Antibody [NBP2-14999] - SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Islet 1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Islet-1-Antibody-Western-Blot-NBP2-14999-img0006.jpg)
![Immunohistochemistry-Paraffin: Islet-1 Antibody [NBP2-14999] - Rat fore brain. Islet 1 antibody dilution: 1:100. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Islet-1-Antibody-Immunohistochemistry-Paraffin-NBP2-14999-img0001.jpg)
![Western Blot: Islet-1 Antibody (2E7) [H00003670-M05] - Analysis of ISL1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Islet-1-Antibody-2E7-Western-Blot-H00003670-M05-img0004.jpg "Western Blot: Islet-1 Antibody (2E7) [H00003670-M05] - Analysis of ISL1 expression in PC-12 (Cat # L012V1).")
![Immunocytochemistry/Immunofluorescence: Islet-1 Antibody (2E7) [H00003670-M05] - Analysis of monoclonal antibody to ISL1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Islet-1-Antibody-2E7-Immunocytochemistry-Immunofluorescence-H00003670-M05-img0003.jpg)
![Western Blot: Islet-1 Antibody (1H9) [NBP1-47537] - Analysis using ISL1 mouse mAb against full-length ISL1 (aa1-349)-hIgGFc transfected HEK293 cell lysate(1).](http://images.novusbio.com/fullsize/Islet-1-Antibody-1H9-Western-Blot-NBP1-47537-img0010.jpg "Western Blot: Islet-1 Antibody (1H9) [NBP1-47537] - Analysis using ISL1 mouse mAb against full-length ISL1 (aa1-349)-hIgGFc transfected HEK293 cell lysate(1).")
![Immunocytochemistry/Immunofluorescence: Islet-1 Antibody (1H9) [NBP1-47537] - Analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using ISL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Islet-1-Antibody-1H9-Immunocytochemistry-Immunofluorescence-NBP1-47537-img0007.jpg)
![Immunohistochemistry-Paraffin: Islet-1 Antibody (1H9) [NBP1-47537] - Analysis of human lung cancer (left) and cervical carcinoma (right), showing nuclear localization using ISL1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Islet-1-Antibody-1H9-Immunohistochemistry-Paraffin-NBP1-47537-img0009.jpg)
![Western Blot: Islet-1 Antibody (1G9Q8) [NBP3-15315] - Western blot analysis of extracts of various cell lines, using Islet-1 Rabbit mAb (NBP3-15315) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Islet-1-Antibody-1G9Q8-Western-Blot-NBP3-15315-img0002.jpg "Western Blot: Islet-1 Antibody (1G9Q8) [NBP3-15315] - Western blot analysis of extracts of various cell lines, using Islet-1 Rabbit mAb (NBP3-15315) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: Islet-1 Antibody (1G9Q8) [NBP3-15315] - Immunohistochemistry of paraffin-embedded mouse fetal Heart using Islet-1 Rabbit mAb (NBP3-15315) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Islet-1-Antibody-1G9Q8-Immunohistochemistry-Paraffin-NBP3-15315-img0001.jpg)
![Immunohistochemistry: Islet-1 Antibody (1A1) [NBP3-26193] - Image of Islet-1 Antibody (1A1) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26193_rabbit-islet-1-mab-1a1-26220241522421.jpg "Immunohistochemistry: Islet-1 Antibody (1A1) [NBP3-26193] - Image of Islet-1 Antibody (1A1) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Islet-1 Antibody (1A1) [NBP3-26193] - Image of Islet-1 Antibody (1A1) diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26193_rabbit-islet-1-mab-1a1-262202415475645.jpg)
![Western Blot: Islet-1 Antibody (1A1) [NBP3-26193] - Positive Western Blot detected in: 293T whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate. All lanes: Islet-1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40 kDa Observed band size: 40 kDa](http://images.novusbio.com/fullsize/nbp3-26193_rabbit-islet-1-mab-1a1-28220241152516.jpg)
![Western Blot: Islet-1 Antibody (2D8) [NBP2-45452] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Islet-1.](http://images.novusbio.com/fullsize/Islet-1-Antibody-2D8-Western-Blot-NBP2-45452-img0002.jpg "Western Blot: Islet-1 Antibody (2D8) [NBP2-45452] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Islet-1.")
![Immunocytochemistry/Immunofluorescence: Islet-1 Antibody (CL11651) [NBP3-15121] - Staining of HeLa cells using the Anti-ISL1 monoclonal antibody, showing localization to nucleoplasm and cytosol in green. Microtubule probe is visualized in red.](http://images.novusbio.com/fullsize/Islet-1-Antibody-CL11651-Immunocytochemistry-Immunofluorescence-NBP3-15121-img0001.jpg "Immunocytochemistry/Immunofluorescence: Islet-1 Antibody (CL11651) [NBP3-15121] - Staining of HeLa cells using the Anti-ISL1 monoclonal antibody, showing localization to nucleoplasm and cytosol in green. Microtubule probe is visualized in red.")
![Western Blot: Islet 1 Overexpression Lysate (Adult Normal) [NBL1-12050] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Islet 1.](http://images.novusbio.com/fullsize/Islet-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12050-img0002.jpg "Western Blot: Islet 1 Overexpression Lysate (Adult Normal) [NBL1-12050] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Islet 1.")
![Western Blot: Islet-1 Antibody (4F6) [NBP3-26191] - Positive Western Blot detected in: Hela whole cell lysate, SH-SY5Y whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate. All lanes: Islet-1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40 kDa Observed band size: 39 kDa](http://images.novusbio.com/fullsize/nbp3-26191_rabbit-islet-1-mab-4f6-282202411351621.jpg "Western Blot: Islet-1 Antibody (4F6) [NBP3-26191] - Positive Western Blot detected in: Hela whole cell lysate, SH-SY5Y whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate. All lanes: Islet-1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40 kDa Observed band size: 39 kDa")
![ELISA: Human Islet-1 ELISA Kit (Colorimetric) [NBP3-39633] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39633_human-islet-1-elisa-kit-colorimetric-2110202415351251.png "ELISA: Human Islet-1 ELISA Kit (Colorimetric) [NBP3-39633] - Standard Curve Reference")