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ewsr1 Products By Gene Name

15 Results for "ewsr1"

NB200-182

EWSR1 Antibody

(1 Publication)

100 ul

NB200-182

Western Blot: EWSR1 Antibody [NB200-182] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T lysate.

Immunohistochemistry-Paraffin: EWSR1 Antibody [NB200-182] - Detection of human EWS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EWS (NB200-182). Detection: DAB

Western Blot: EWSR1 Antibody [NB200-182] - Detection of human and mouse EWS by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody NB200-182 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Immunoprecipitation: EWSR1 Antibody [NB200-182] - Detection of human EWS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EWS antibody NB200-182 (lot NB200-182-2) used for IP at 3 ug per reaction. EWS was also immunoprecipitated by a previous lot of this antibody (lot NB200-182-1). For blotting immunoprecipitated EWS, NB200-182 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Simple Western: EWSR1 Antibody [NB200-182] - Simple Western lane view shows HeLa, HEK293T, and HepG2 whole cell lysate (WCL). A specific band was detected for EWSR1 antibody (NBP1-49701) at approximately 116-140 kDa (as indicated) using 10 ug/mL of EWSR1 antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

The cytoplasmic mislocalization induced by P525L causes reduced FUS binding to several ALS-associated RBPs, promoting aggregation. a, b Western blot analysis of FUS protein interactors in a LL & b SL neurons after FUS-eGFP immunoprecipitation reveals differential interactions with several ALS-associated partners. n = 4. Error bars indicate SEM. *, **, & *** Correspond to p < 0.05, 0.01, & 0.001, respectively. c In vitro phase separation assay showing fibrillization of purified P525L LL FUS-eGFP protein in the presence or absence of distinct RBPs. Investigated RBPs effectively prevent FUS fibril formation. d Fluorescence recovery after photobleaching (FRAP) was used to assess the dynamics of P525L LL FUS at the tested conditions for the indicated time points. RBPs promote the maintenance of a liquid-like behavior. e Co-localization of P525L LL FUS with the reported RBPs. Scale bar 5 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30937520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

The cytoplasmic mislocalization induced by P525L causes reduced FUS binding to several ALS-associated RBPs, promoting aggregation. a, b Western blot analysis of FUS protein interactors in a LL & b SL neurons after FUS-eGFP immunoprecipitation reveals differential interactions with several ALS-associated partners. n = 4. Error bars indicate SEM. *, **, & *** Correspond to p < 0.05, 0.01, & 0.001, respectively. c In vitro phase separation assay showing fibrillization of purified P525L LL FUS-eGFP protein in the presence or absence of distinct RBPs. Investigated RBPs effectively prevent FUS fibril formation. d Fluorescence recovery after photobleaching (FRAP) was used to assess the dynamics of P525L LL FUS at the tested conditions for the indicated time points. RBPs promote the maintenance of a liquid-like behavior. e Co-localization of P525L LL FUS with the reported RBPs. Scale bar 5 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30937520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Autophagic clearance of aberrantly accumulated cytoplasmic FUS restores protein homeostasis & ameliorates survival of SL P525L iPSC-derived neurons. a Confocal micrographs showing FUS-eGFP distribution before & after Torkinib treatment (above). Arrowhead indicates FUS-eGFP cytoplasmic accumulation in untreated neurites; arrow shows reduced FUS-eGFP cytoplasmic signal following torkinib treatment. Quantification of cytoplasmic FUS-eGFP signal intensity in acquired images (below) confirms clearance of mislocalized FUS-eGFP protein. Scale bar = 10 µm. b FRAP analysis performed on untreated versus torkinib-treated neurons shows comparable dynamics of FUS-eGFP recovery. n = 3. Error bars indicate SEM. CHX = cycloheximide. c WES capillary electrophoresis & d corresponding quantification of the indicated proteins in P525L SL neurons before & after torkinib treatment. Autophagy stimulation restores physiological levels. n = 4. Error bars indicate SEM. * & ** Correspond to p < 0.05 & 0.01, respectively. e 6 h of torkinib reduces apoptotic cell death identified by cleaved Caspase 3 staining. Scale bar = 50 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30937520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB200-182AF350
Alexa Fluor 405	NB200-182AF405
Alexa Fluor 488	NB200-182AF488
Alexa Fluor 532	NB200-182AF532
Alexa Fluor 594	NB200-182AF594
Alexa Fluor 647	NB200-182AF647
Alexa Fluor 700	NB200-182AF700
Alexa Fluor 750	NB200-182AF750
Allophycocyanin	NB200-182APC
Biotin	NB200-182B
CoraFluor 1	NB200-182CL1
DyLight 350	NB200-182UV
DyLight 405	NB200-182V
DyLight 488	NB200-182G
DyLight 550	NB200-182R
DyLight 594	NB200-182DL594
DyLight 650	NB200-182C
DyLight 680	NB200-182FR
DyLight 755	NB200-182IR
FITC	NB200-182F
HRP	NB200-182H
Janelia Fluor 525	NB200-182JF525
Janelia Fluor 549	NB200-182JF549
Janelia Fluor 585	NB200-182JF585
Janelia Fluor 635	NB200-182JF635
Janelia Fluor 646	NB200-182JF646
Janelia Fluor 669	NB200-182JF669
PE	NB200-182PE
PerCP	NB200-182PCP

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NBP1-92686

EWSR1 Antibody (5H7)

(2 Publications)

0.1 ml

NBP1-92686

Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T and huh-7 lysate.

Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (5H7) [NBP1-92686] - HeLa cell cultures stained with antibody NBP1-92686 (green) and chicken antibody to vimentin (NB300-223, red). Blue is a DNA stain, and it is clear that in these cells EWSR1 is localized along with the DNA in the nucleus.

Western Blot: EWSR1 Antibody (5H7) [NBP1-92686] - Analysis of different cell line lysates using EWSR1 antibody, dilution 1:1000 (Green): [1] protein standard (Red), [2] HeLa, [3] HEK293, [4] mouse NIH-3T3, [5] rat PC12, [6] equine NBL6 and [7] canine A72 cells. The strong band at ~80kDa corresponds to the EWS protein seen in all species tested.

Reactivity:

Hu, Mu, Rt, Ca, Eq

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Eq

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-92686AF350
Alexa Fluor 405	NBP1-92686AF405
Alexa Fluor 488	NBP1-92686AF488
Alexa Fluor 532	NBP1-92686AF532
Alexa Fluor 594	NBP1-92686AF594
Alexa Fluor 647	NBP1-92686AF647
Alexa Fluor 700	NBP1-92686AF700
Alexa Fluor 750	NBP1-92686AF750
Biotin	NBP1-92686B
CoraFluor 1	NBP1-92686CL1
DyLight 350	NBP1-92686UV
DyLight 405	NBP1-92686V
DyLight 488	NBP1-92686G
DyLight 550	NBP1-92686R
DyLight 594	NBP1-92686DL594
DyLight 650	NBP1-92686C
DyLight 680	NBP1-92686FR
DyLight 755	NBP1-92686IR
FITC	NBP1-92686F
HRP	NBP1-92686H
Janelia Fluor 525	NBP1-92686JF525
Janelia Fluor 549	NBP1-92686JF549
Janelia Fluor 585	NBP1-92686JF585
Janelia Fluor 635	NBP1-92686JF635
Janelia Fluor 646	NBP1-92686JF646
Janelia Fluor 669	NBP1-92686JF669
mFluor Violet 450 SE	NBP1-92686MFV450
mFluor Violet 500 SE	NBP1-92686MFV500
mFluor Violet 610 SE	NBP1-92686MFV610

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NBP2-16389

EWSR1 Antibody

0.1 ml

NBP2-16389

Western Blot: EWSR1 Antibody [NBP2-16389] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EWSR1 antibody diluted at a dilution of 1:1000.

Immunocytochemistry/Immunofluorescence: EWSR1 Antibody [NBP2-16389] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Paraffin-embedded mouse duodenum. EWSR1 antibody dilution: 1:500.

Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Immunohistochemical analysis of paraffin-embedded cal27 xenograft, using antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Paraffin-embedded rat fore brain. EWSR1 antibody dilution: 1:500.

Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Paraffin-embedded mouse testis. EWSR1 antibody dilution: 1:500.

Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-16389] - Paraffin-embedded mouse prostate. EWSR1 antibody dilution: 1:500.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Pm, RM

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Pm, RM

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-16845

EWSR1 Antibody (9G3H1)

100 ul

NBP3-16845

Western Blot: EWSR1 Antibody (9G3H1) [NBP3-16845] - Western blot analysis of extracts of various cell lines, using EWSR1 Rabbit mAb (NBP3-16845) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunofluorescence analysis of U-2 OS cells using EWSR1 Rabbit mAb (NBP3-16845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry of paraffin-embedded mouse testis using EWSR1 Rabbit mAb (NBP3-16845) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunofluorescence analysis of C6 cells using EWSR1 Rabbit mAb (NBP3-16845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunofluorescence analysis of NIH-3T3 cells using EWSR1 Rabbit mAb (NBP3-16845) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry of paraffin-embedded rat lung using EWSR1 Rabbit mAb (NBP3-16845) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: EWSR1 Antibody (9G3H1) [NBP3-16845] - Immunohistochemistry of paraffin-embedded human appendix using EWSR1 Rabbit mAb (NBP3-16845) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-49380

EWSR1 Antibody

0.1 ml

NBP2-49380

Western Blot: EWSR1 Antibody [NBP2-49380] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue

Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-49380] - Staining of human skin.

Immunohistochemistry: EWSR1 Antibody [NBP2-49380] - Staining of human colon shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-49380] - Staining of human skeletal muscle shows low expression as expected.

Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-49380] - Staining of human testis shows high expression.

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-49380] - Staining in human testis and skeletal muscle tissues using anti-EWSR1 antibody. Corresponding EWSR1 RNA-seq data are presented for the same tissues.

<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-49380] - Staining of human colon, liver, skin and testis using Anti-EWSR1 antibody NBP2-49380 (A) shows similar protein distribution across tissues to independent antibody NBP2-49020 (B).

Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-49380] - Staining of human liver.

Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP2-49380] - Staining of human colon.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-28798

EWSR1 Antibody

(1 Publication)

0.1 mg

NBP1-28798

Western Blot: EWSR1 Antibody [NBP1-28798] - (0.3ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP1-28798] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Immunohistochemistry-Paraffin: EWSR1 Antibody [NBP1-28798] - (7ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Reactivity:

Hu, Mu, Rt, Po, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Alt Catalog#

IMG-31193

Alt Catalog# IMG-31193

Conjugate:

Unconjugated

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NBP3-14931

EWSR1 Antibody (S09-3A7)

100 ul

NBP3-14931

Western Blot: EWSR1 Antibody (S09-3A7) [NBP3-14931] - Western blot detection of EWSR1 in MCF7, 3T3, Hela cell lysates using EWSR1 Rabbit mAb (1:1000 diluted). Predicted band size: 69KDa. Observed band size: 85KDa.

Immunocytochemistry/Immunofluorescence: EWSR1 Antibody (S09-3A7) [NBP3-14931] - Immunofluorescence of EWSR1 (green) in Hela cells using EWSR1 Rabbit mAb at dilution 1/50, and DAPI (blue)

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NB200-183

EWSR1 Antibody

(1 Publication)

100 ul

NB200-183

Western Blot: EWSR1 Antibody [NB200-183] - Detection of Human and Mouse EWS by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody NB200-183 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

<b>Independent Antibodies Validation. </b>Immunoprecipitation: EWSR1 Antibody [NB200-183] - Detection of human EWSR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EWSR1 antibody NB200-183 used for IP at 6 ug per reaction. EWSR1 was also immunoprecipitated by rabbit anti-EWSR1 antibody NB200-182. For blotting immunoprecipitated EWSR1, NB200-183 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB200-183AF350
Alexa Fluor 405	NB200-183AF405
Alexa Fluor 488	NB200-183AF488
Alexa Fluor 532	NB200-183AF532
Alexa Fluor 594	NB200-183AF594
Alexa Fluor 647	NB200-183AF647
Alexa Fluor 700	NB200-183AF700
Alexa Fluor 750	NB200-183AF750
Allophycocyanin	NB200-183APC
Biotin	NB200-183B
CoraFluor 1	NB200-183CL1
DyLight 350	NB200-183UV
DyLight 405	NB200-183V
DyLight 488	NB200-183G
DyLight 550	NB200-183R
DyLight 594	NB200-183DL594
DyLight 650	NB200-183C
DyLight 680	NB200-183FR
DyLight 755	NB200-183IR
FITC	NB200-183F
HRP	NB200-183H
Janelia Fluor 525	NB200-183JF525
Janelia Fluor 549	NB200-183JF549
Janelia Fluor 585	NB200-183JF585
Janelia Fluor 635	NB200-183JF635
Janelia Fluor 646	NB200-183JF646
Janelia Fluor 669	NB200-183JF669
PE	NB200-183PE
PerCP	NB200-183PCP

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NB100-75256

EWSR1 Antibody

0.05 mg

NB100-75256

Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. (anti-EWSR1)

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP3-19628

EWSR1 Antibody (S04-4B3)

100 ul

NBP3-19628

Western Blot: EWSR1 Antibody (S04-4B3) [NBP3-19628] - Western blot detection of EWSR1 in Hela cell lysates using NBP3-19628 (1:1000 diluted). Predicted band size: 69kDa. Observed band size: 85kDa.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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H00002130-Q01

Recombinant Human EWSR1 GST (N-Term) Protein

10 μg

H00002130-Q01

12.5% SDS-PAGE Stained with Coomassie Blue.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

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NBP2-49020

EWSR1 Antibody

0.1 ml

NBP2-49020

Analysis in human cell line Daudi.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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