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![Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.Lane 1: CDK6 transfected lysate(36.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Cdk6-Antibody-8H4-Western-Blot-H00001021-M01-img0015.jpg "Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody (M01), clone 8H4.Lane 1: CDK6 transfected lysate(36.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of monoclonal antibody to CDK6 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Cdk6-Antibody-8H4-Immunocytochemistry-Immunofluorescence-H00001021-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of monoclonal antibody to CDK6 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Cdk6-Antibody-8H4-Immunohistochemistry-Paraffin-H00001021-M01-img0011.jpg)
![Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - CDK6 monoclonal antibody (M01), clone 8H4. Analysis of CDK6 expression in PC-12.](http://images.novusbio.com/fullsize/Cdk6-Antibody-8H4-Western-Blot-H00001021-M01-img0013.jpg)
![Western Blot: Cdk6 Antibody (8H4) [H00001021-M01] - CDK6 monoclonal antibody (M01), clone 8H4 Analysis of CDK6 expression in Jurkat.](http://images.novusbio.com/fullsize/Cdk6-Antibody-8H4-Western-Blot-H00001021-M01-img0014.jpg)
![Proximity Ligation Assay: Cdk6 Antibody (8H4) [H00001021-M01] - Analysis of protein-protein interactions between CDK2 and CDK6. HeLa cells were stained with anti-CDK2 rabbit purified polyclonal 1:1200 and anti-CDK6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cdk6-Antibody-8H4-Proximity-Ligation-Assay-H00001021-M01-img0007.jpg)
![ELISA: Cdk6 Antibody (8H4) [H00001021-M01] - Detection limit for recombinant GST tagged CDK6 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cdk6-Antibody-8H4-ELISA-H00001021-M01-img0016.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Analysis in human placenta and skeletal muscle tissues. Corresponding CDK6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Analysis in human placenta and skeletal muscle tissues. Corresponding CDK6 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Cdk6 Antibody [NBP1-87262] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP1-87262-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Cdk6 Antibody [NBP1-87262] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87262-img0019.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0027.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cdk6 Antibody [NBP1-87262] - Analysis in human cell lines U2OS and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP1-87262-img0020.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human lymph node shows moderate to strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0024.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0025.jpg)
![Immunohistochemistry-Paraffin: Cdk6 Antibody [NBP1-87262] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunohistochemistry-Paraffin-NBP1-87262-img0026.jpg)
![Western Blot: Cdk6 Antibody [NBP2-92967] - analysis of lysates from HeLa cells, using CDK6 Rabbit pAb at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25Ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-92967_rabbit-polyclonal-cdk6-antibody-western-blot-1542024133214..jpg "Western Blot: Cdk6 Antibody [NBP2-92967] - analysis of lysates from HeLa cells, using CDK6 Rabbit pAb at 1:900 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25Ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 30s.")
![Western Blot: Cdk6 Antibody [NBP2-15846] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cdk6 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP2-15846-img0004.jpg "Western Blot: Cdk6 Antibody [NBP2-15846] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cdk6 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Cdk6 Antibody [NBP2-15846] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK6 protein stained by CDK6 antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15846-img0003.jpg)
![Western Blot: Cdk6 Antibody [NBP2-15846] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP2-15846-img0001.jpg)
![Western Blot: Cdk6 Antibody [NBP2-15846] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK6 antibody [N3C3] diluted at 1:500.](http://images.novusbio.com/fullsize/Cdk6-Antibody-Western-Blot-NBP2-15846-img0002.jpg)
![Immunoprecipitation: Cdk6 Antibody (8G3) [NBP3-26663] - Immunoprecipitating Cdk6 in K562 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26663 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26663 (2ug)+ K562 whole cell lysate (500ug). Lane 3: K562 whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26663_rabbit-cdk6-mab-8g3-2622024132371.jpg "Immunoprecipitation: Cdk6 Antibody (8G3) [NBP3-26663] - Immunoprecipitating Cdk6 in K562 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26663 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26663 (2ug)+ K562 whole cell lysate (500ug). Lane 3: K562 whole cell lysate (10ug).")
![Western Blot: Cdk6 Antibody (8G3) [NBP3-26663] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, 293 whole cell lysate. All lanes: Cdk6 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 37 kDa Observed band size: 37 kDa](http://images.novusbio.com/fullsize/nbp3-26663_rabbit-cdk6-mab-8g3-28220241135160.jpg)
![Recombinant Human Cdk6 Protein [H00001021-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001021-P02-1.jpg "Recombinant Human Cdk6 Protein [H00001021-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Cdk6 Antibody (0O6X7) [NBP3-15249] - Western blot analysis of extracts of various cell lines, using Cdk6 Rabbit mAb (NBP3-15249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Cdk6-Antibody-0O6X7-Western-Blot-NBP3-15249-img0001.jpg "Western Blot: Cdk6 Antibody (0O6X7) [NBP3-15249] - Western blot analysis of extracts of various cell lines, using Cdk6 Rabbit mAb (NBP3-15249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![ELISA: Human Cdk6 ELISA Kit (Colorimetric) [NBP2-75177] - Samples were spiked with high concentrations of Human Cdk6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75177_human-cdk6-elisa-kit-colorimetric-132202416184844.jpg "ELISA: Human Cdk6 ELISA Kit (Colorimetric) [NBP2-75177] - Samples were spiked with high concentrations of Human Cdk6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Cdk6 ELISA Kit (Colorimetric) [NBP2-75177] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75177_human-cdk6-elisa-kit-colorimetric-4320249151675.jpg)
![Western Blot: Cdk6 Overexpression Lysate (Adult Normal) [NBP2-09735] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cdk6.](http://images.novusbio.com/fullsize/Cdk6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09735-img0001.jpg "Western Blot: Cdk6 Overexpression Lysate (Adult Normal) [NBP2-09735] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cdk6.")
![Western Blot: Cdk6 Overexpression Lysate (Adult Normal) [NBL1-09044] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cdk6.](http://images.novusbio.com/fullsize/Cdk6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09044-img0002.jpg "Western Blot: Cdk6 Overexpression Lysate (Adult Normal) [NBL1-09044] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cdk6.")