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Human Cdk6 ELISA Kit (Colorimetric)

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ELISA: Human Cdk6 ELISA Kit (Colorimetric) [NBP2-75177] - Samples were spiked with high concentrations of Human Cdk6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human Cdk6 ELISA Kit (Colorimetric) [NBP2-75177] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

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Human Cdk6 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CDK6 in samples. No significant cross-reactivity or interference between Human CDK6 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.55%
Intra-Assay
CV% < 5.19%
Spike Recovery
85-103%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CDK6

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Cdk6 ELISA Kit (Colorimetric)

  • Cdk6
  • CDKN6
  • Cell division protein kinase 6
  • cyclin-dependent kinase 6
  • EC 2.7.11
  • EC 2.7.11.22
  • PLSTIREMGC59692
  • Serine/threonine-protein kinase PLSTIRE

Background

Cdk6 is encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CDK6