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![Western Blot: CASK Antibody [NBP2-41181] - Western blot analysis of CASK in mouse brain tissue lysate with CASK antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/CASK-Antibody-Western-Blot-NBP2-41181-img0001.jpg "Western Blot: CASK Antibody [NBP2-41181] - Western blot analysis of CASK in mouse brain tissue lysate with CASK antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP2-41181] - Immunofluorescence of CASK in human brain tissue with CASK antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41181-img0002.jpg)
![Simple Western: CASK Antibody [NBP2-41181] - 0.6 mg/ml protein lysates from differentiating human neuroepithelial stem cells run on Simple Western with 1:500 CASK.Ab (blue) and 1:1000 CASK.Ab (pink) and an actin antibody as positive control. The CASK peak is located at 116 kDa and actin at 38 kDa. Image is courtesy of customer.](http://images.novusbio.com/fullsize/CASK-Antibody-Simple-Western-NBP2-41181-img0003.jpg)
![Simple Western: CASK Antibody - BSA Free [NBP2-41181] - Presynaptic effect is limited to inhibitory VGAT presynaptic marker.a Deconvolution of cell-type populations underlying bulk RNA-sequence samples from all cell lines. b Quantification of VGlut & Homer-1 particle size (n = 4 per cell line). c Representative confocal microscopy images of neurons differentiated for 28 days & immunostained for inhibitory VGAT (green), Homer-1 (red), & Hoechst (blue). Scale = 10 µm. d Quantification of VGAT & Homer-1 particle size (n = 3 per cell line). Statistical differences between cell lines were calculated using ANOVA with post hoc Tukey HSD. *p < 0.05, **p < 0.01, ***p < 0.001. Asterisks are color-coded according to case cell lines. e In vivo concentration of GABA in the DLPFC, MFC, & putamen of ASDCASK_SS & his cotwin in relation to a typical developed control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32929080), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-41181_rabbit-polyclonal-cask-antibody-31020241612227.jpg)
![Immunocytochemistry/ Immunofluorescence: CASK Antibody - BSA Free [NBP2-41181] - Immunofluorescence of CASK in human brain tissue with CASK antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41181_rabbit-polyclonal-cask-antibody-2711202420441162.jpg)
![Immunocytochemistry/ Immunofluorescence: CASK Antibody - BSA Free [NBP2-41181] - Immunofluorescence of CASK in human brain tissue with CASK antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41181_rabbit-polyclonal-cask-antibody-271120242092052.jpg)
![Western Blot: CASK Antibody [NBP2-76922] - Western blot analysis of CASK on different cell lysate using anti-CASK antibody at 1/500 dilution.Positive control:Lane1: SiHaLane2: A549](http://images.novusbio.com/fullsize/CASK-Antibody-Western-Blot-NBP2-76922-img0001.jpg "Western Blot: CASK Antibody [NBP2-76922] - Western blot analysis of CASK on different cell lysate using anti-CASK antibody at 1/500 dilution.Positive control:Lane1: SiHaLane2: A549")
![Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP2-76922] - ICC staining CASK in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76922-img0003.jpg)
![Immunohistochemistry: CASK Antibody [NBP2-76922] - Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CASK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunohistochemistry-NBP2-76922-img0007.jpg)
![Flow Cytometry: CASK Antibody [NBP2-76922] - Flow cytometric analysis of HT-29 cells with CASK antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CASK-Antibody-Flow-Cytometry-NBP2-76922-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP2-76922] - ICC staining CASK in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76922-img0002.jpg)
![Immunohistochemistry: CASK Antibody [NBP2-76922] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CASK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunohistochemistry-NBP2-76922-img0004.jpg)
![Immunohistochemistry: CASK Antibody [NBP2-76922] - Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-CASK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunohistochemistry-NBP2-76922-img0005.jpg)
![Immunohistochemistry: CASK Antibody [NBP2-76922] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-CASK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunohistochemistry-NBP2-76922-img0006.jpg)
![Western Blot: CASK Antibody [NBP3-13185] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CASK antibody (NBP3-13185) diluted at 1:5000.](http://images.novusbio.com/fullsize/CASK-Antibody-Western-Blot-NBP3-13185-img0001.jpg "Western Blot: CASK Antibody [NBP3-13185] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CASK antibody (NBP3-13185) diluted at 1:5000.")
![Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP3-13185] - CASK antibody detects CASK protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: CASK protein stained by CASK antibody (NBP3-13185) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13185-img0004.jpg)
![Immunohistochemistry-Paraffin: CASK Antibody [NBP3-13185] - CASK antibody detects CASK protein at cytoplasm in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. CASK antibody (NBP3-13185) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CASK-Antibody-Immunohistochemistry-Paraffin-NBP3-13185-img0003.jpg)
![Immunohistochemistry-Paraffin: CASK Antibody [NBP3-13185] - CASK antibody detects CASK protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CASK antibody (NBP3-13185) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/CASK-Antibody-Immunohistochemistry-Paraffin-NBP3-13185-img0002.jpg)
![Western Blot: CASK Antibody [NBP2-15710] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CASK antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CASK-Antibody-Western-Blot-NBP2-15710-img0004.jpg "Western Blot: CASK Antibody [NBP2-15710] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CASK antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP2-15710] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15710-img0002.jpg)
![Immunohistochemistry-Paraffin: CASK Antibody [NBP2-15710] - Paraffin-embedded mouse lung. CASK antibody diluted at 1:500.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunohistochemistry-Paraffin-NBP2-15710-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP1-86673] - Staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86673-img0010.jpg "Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP1-86673] - Staining of human cell line U-251 MG shows localization to nucleoplasm.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CASK Antibody [NBP1-86673] - Staining in human duodenum and testis tissues using anti-CASK antibody. Corresponding CASK RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunohistochemistry-Paraffin-NBP1-86673-img0009.jpg)
![Immunohistochemistry-Paraffin: CASK Antibody [NBP1-86673] - Staining of human duodenum shows high expression.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunohistochemistry-Paraffin-NBP1-86673-img0007.jpg)
![Immunohistochemistry-Paraffin: CASK Antibody [NBP1-86673] - Staining of human testis shows low expression as expected.](http://images.novusbio.com/fullsize/CASK-Antibody-Immunohistochemistry-Paraffin-NBP1-86673-img0008.jpg)
![Western Blot: CASK Antibody [NB300-321] - Analysis of AtT20 cell lysate.](http://images.novusbio.com/fullsize/CASK-Antibody-Western-Blot-NB300-321-img0004.jpg "Western Blot: CASK Antibody [NB300-321] - Analysis of AtT20 cell lysate.")
![Western Blot: CASK Antibody (A1C5-R) [NBP3-32108] - Western blot analysis of CASK on different lysates with Mouse anti-CASK antibody (NBP3-32108) at 1/2,000 dilution. Lane 1: SiHa cell lysate Lane 2: A549 cell lysate Lane 3: U-87 MG cell lysate Lane 4: Neuro-2a cell lysate Lane 5: Human brain tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32108) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32108_mouse-cask-mab-a1c5-r-278202416331294.jpg "Western Blot: CASK Antibody (A1C5-R) [NBP3-32108] - Western blot analysis of CASK on different lysates with Mouse anti-CASK antibody (NBP3-32108) at 1/2,000 dilution. Lane 1: SiHa cell lysate Lane 2: A549 cell lysate Lane 3: U-87 MG cell lysate Lane 4: Neuro-2a cell lysate Lane 5: Human brain tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32108) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CASK Antibody (A1C5-R) [NBP3-32108] - Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Mouse anti-CASK antibody (NBP3-32108) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32108) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32108_mouse-cask-mab-a1c5-r-29820241492694.jpg)
![Western Blot: CASK Antibody [NBP3-38000] - Western blot analysis of various lysates using CASK Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38000_rabbit-cask-pab-912202414154344.jpg "Western Blot: CASK Antibody [NBP3-38000] - Western blot analysis of various lysates using CASK Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: CASK Antibody [NBP3-38000] - Immunofluorescence analysis of U2OS cells using CASK Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38000_rabbit-cask-pab-912202415133495.jpg)