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![Simple Western: BAG3 Antibody [NBP2-27398] - Simple Western lane view shows a specific band for BAG3 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/BAG3-Antibody-Simple-Western-NBP2-27398-img0006.jpg "Simple Western: BAG3 Antibody [NBP2-27398] - Simple Western lane view shows a specific band for BAG3 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: BAG3 Antibody [NBP2-27398] - IHC of BAG3 in IR myocardium. BAG3 expression is significantly decreased in the STZ group than those in the CON group. BAG3 expression is also significantly reduced in the IR CON and IR STZ groups as compared with the CON group. IR STZ hearts further depress BAG3 expression than IR CON hearts. Data are expressed as mean +/- SEM (n = 3) using the single values. * p < 0.05 vs. CON, a p < 0.05 vs. STZ, b p < 0.05 vs. IR CON. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2076-3921/9/8/679) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-NBP2-27398-img0009.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: BAG3 Antibody [NBP2-27398] - Western blot of BAG3 in IR myocardium. BAG3 expression is significantly decreased in the STZ group than those in the CON group. BAG3 expression is also significantly reduced in the IR CON and IR STZ groups as compared with the CON group. IR STZ hearts further depress BAG3 expression than IR CON hearts. Data are expressed as mean +/- SEM (n = 3) using the single values. * p < 0.05 vs. CON, a p < 0.05 vs. STZ, b p < 0.05 vs. IR CON. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2076-3921/9/8/679) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BAG3-Antibody-Western-Blot-NBP2-27398-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP2-27398] - Antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27398-img0005.jpg)
![Western Blot: BAG3 Antibody [NBP2-27398] - Analysis of BAG3 using NBP2-27398 at 1:2000. Tissue lysates, normalized for total protein (20 ug/lane), were from a 4 week old male mouse. BAG-3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.](http://images.novusbio.com/fullsize/BAG3-Antibody-Western-Blot-NBP2-27398-img0001.jpg)
![Immunohistochemistry-Frozen: BAG3 Antibody [NBP2-27398] - Frozen mouse muscle tissue section stained for BAG-3 expression using NBP2-27398 at 1:2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG-3 localizes with Z-disk proteins.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Frozen-NBP2-27398-img0002.jpg)
![Western Blot: BAG3 Antibody [NBP2-27398] - Mesenteric vascular Western blot graph (A) & analysis of 4-HNE (B), CaSR (C), p-eNOS (D) & BAG3 (E) are demonstrated. Microvascular reactivity from mesenteric arterioles to a vasoconstrictor (norepinephrine, NE, F) & vasodilator (acetylcholine, Ach, G) is displayed in CON & STZ groups. All data are presented as the mean ± SEM (n = 3) using the single values in each test. * p < 0.05 vs. CON group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32751309), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-27398_rabbit-polyclonal-bag3-antibody-310202415175241.jpg)
![Western Blot: BAG3 Antibody [NBP2-15543] - BAG3 antibody detects BAG3 protein by Western blot analysis. A. 50 ug mouse heart lysate/extract 7. 5 % SDS-PAGEBAG3 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/BAG3-Antibody-Western-Blot-NBP2-15543-img0006.jpg "Western Blot: BAG3 Antibody [NBP2-15543] - BAG3 antibody detects BAG3 protein by Western blot analysis. A. 50 ug mouse heart lysate/extract 7. 5 % SDS-PAGEBAG3 antibody dilution: 1:1000.")
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-15543] - BAG3 in high-grade serous carcinoma. 1:100 dilution, pH=6 retrieval buffer, Dako FLEX+ protocol. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP2-15543-img0007.jpg)
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-15543] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP2-15543-img0004.jpg)
![Western Blot: BAG3 Antibody [NBP2-15543] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BAG3 antibody (NBP2-15543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15543_rabbit-polyclonal-bag3-antibody-91020242015712.jpg)
![Western Blot: BAG3 Antibody [NBP1-86429] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Skeletal muscle tissue](http://images.novusbio.com/fullsize/BAG3-Antibody-Western-Blot-NBP1-86429-img0004.jpg "Western Blot: BAG3 Antibody [NBP1-86429] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Skeletal muscle tissue")
![Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP1-86429] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86429-img0005.jpg)
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0014.jpg)
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human liver.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0007.jpg)
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human kidney.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0008.jpg)
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human testis.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0009.jpg)
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human cerebellum, prostate, skeletal muscle and skin using Anti-BAG3 antibody NBP1-86429 (A) shows similar protein distribution across tissues to independent antibody NBP1-86442 (B).](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0011.jpg)
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0012.jpg)
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0013.jpg)
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86429-img0015.jpg)
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human cerebellum, prostate, skeletal muscle and skin using Anti-BAG3 antibody NBP1-86442 (A) shows similar protein distribution across tissues to independent antibody NBP1-86429 (B).](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86442-img0012.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human cerebellum, prostate, skeletal muscle and skin using Anti-BAG3 antibody NBP1-86442 (A) shows similar protein distribution across tissues to independent antibody NBP1-86429 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP1-86442] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86442-img0004.jpg)
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86442-img0015.jpg)
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86442-img0013.jpg)
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86442-img0014.jpg)
![Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/BAG3-Antibody-Immunohistochemistry-Paraffin-NBP1-86442-img0016.jpg)
![Western Blot: BAG3 Antibody [NBP2-27330] - Analysis of BAG3 in CAPAN-1 human pancreatic cancer cells following heat shock using NBP2-27330 at 1:2000.](http://images.novusbio.com/fullsize/BAG3-Antibody-Western-Blot-NBP2-27330-img0004.jpg "Western Blot: BAG3 Antibody [NBP2-27330] - Analysis of BAG3 in CAPAN-1 human pancreatic cancer cells following heat shock using NBP2-27330 at 1:2000.")
![Western Blot: BAG3 Antibody [NBP2-27330] - Analysis of BAG3 in human pancreatic cencer cell lines using NBP2-27330 at 1:2000.](http://images.novusbio.com/fullsize/BAG3-Antibody-Western-Blot-NBP2-27330-img0002.jpg)
![Western Blot: BAG3 Antibody (8P3W7) [NBP3-16469] - Western blot analysis of extracts of various cell lines, using BAG3 Rabbit mAb (NBP3-16469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/BAG3-Antibody-8P3W7-Western-Blot-NBP3-16469-img0002.jpg "Western Blot: BAG3 Antibody (8P3W7) [NBP3-16469] - Western blot analysis of extracts of various cell lines, using BAG3 Rabbit mAb (NBP3-16469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: BAG3 Antibody (8P3W7) [NBP3-16469] - Immunohistochemistry of paraffin-embedded mouse kidney using BAG3 Rabbit mAb (NBP3-16469) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BAG3-Antibody-8P3W7-Immunohistochemistry-Paraffin-NBP3-16469-img0001.jpg)
![Immunohistochemistry: BAG3 Antibody (3D8) [NBP3-26483] - Image of BAG3 Antibody (3D8) diluted at 1:73.75 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26483_rabbit-bag3-mab-3d8-262202414225211.jpg "Immunohistochemistry: BAG3 Antibody (3D8) [NBP3-26483] - Image of BAG3 Antibody (3D8) diluted at 1:73.75 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: BAG3 Antibody (3D8) [NBP3-26483] - Positive Western Blot detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate. All lanes: BAG3 Antibody at 0.73ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 KDa Observed band size: 80 KDa](http://images.novusbio.com/fullsize/nbp3-26483_rabbit-bag3-mab-3d8-282202411442638.jpg)
![Western Blot: BAG3 Antibody (S01-3F1) [NBP3-14910] - Western blot detection of BAG3 in C6, 3T3, Hela cell lysates using BAG3 Rabbit mAb (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 80kDa.](http://images.novusbio.com/fullsize/BAG3-Antibody-S01-3F1-Western-Blot-NBP3-14910-img0001.jpg "Western Blot: BAG3 Antibody (S01-3F1) [NBP3-14910] - Western blot detection of BAG3 in C6, 3T3, Hela cell lysates using BAG3 Rabbit mAb (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 80kDa.")
![Western Blot: BAG3 Antibody (S05-6D5) [NBP3-19578] - Western blot detection of BAG3 in K562, Rat Brain cell lysates using NBP3-19578 (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 80kDa.](http://images.novusbio.com/fullsize/BAG3-Antibody-S05-6D5-Western-Blot-NBP3-19578-img0001.jpg "Western Blot: BAG3 Antibody (S05-6D5) [NBP3-19578] - Western blot detection of BAG3 in K562, Rat Brain cell lysates using NBP3-19578 (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 80kDa.")