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![Immunocytochemistry/Immunofluorescence: BTBD12 Antibody [NBP1-28680] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-BTBD12 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/BTBD12-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-28680-img0009.jpg "Immunocytochemistry/Immunofluorescence: BTBD12 Antibody [NBP1-28680] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-BTBD12 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: BTBD12 Antibody [NBP1-28680] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human breast infiltrating duct carcinoma using BTBD12 antibody at 1:250 dilution. The signal was developed using HRP-conjugated secondary antibody and DAB reagent which followed counter-staining of the cellular nuclei with hematoxylin. This BTBD12 antibody generated a strong and very specific nuclear staining in a subset of cancerous cells in the tested section.](http://images.novusbio.com/fullsize/BTBD12-Antibody-Immunohistochemistry-Paraffin-NBP1-28680-img0008.jpg)
![Western Blot: BTBD12 Antibody [H00084464-B01P] - Analysis of BTBD12 expression in transfected 293T cell line by BTBD12 polyclonal antibody. Lane 1: BTBD12 transfected lysate(126.61 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BTBD12-Antibody-Western-Blot-H00084464-B01P-img0003.jpg "Western Blot: BTBD12 Antibody [H00084464-B01P] - Analysis of BTBD12 expression in transfected 293T cell line by BTBD12 polyclonal antibody. Lane 1: BTBD12 transfected lysate(126.61 KDa). Lane 2: Non-transfected lysate.")
![Immunoprecipitation: BTBD12 Antibody [NBP1-28679] - Detection of human BTBD12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BTBD12 antibody NBP1-28679 used for IP at 6 ug per reaction. BTBD12 was also immunoprecipitated by another rabbit anti-BTBD12 antibody. For blotting immunoprecipitated BTBD12, NBP1-28679 was used at 3 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BTBD12-Antibody-Immunoprecipitation-NBP1-28679-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: BTBD12 Antibody [NBP1-28679] - Detection of human BTBD12 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BTBD12 antibody NBP1-28679 used for IP at 6 ug per reaction. BTBD12 was also immunoprecipitated by another rabbit anti-BTBD12 antibody. For blotting immunoprecipitated BTBD12, NBP1-28679 was used at 3 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry;SLX4 Antibody[NBP3-29476]Detection of human BTBD12 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BTBD12 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29477_rabbit-slx4-pab-78202410372620.jpg "Immunohistochemistry;SLX4 Antibody[NBP3-29476]Detection of human BTBD12 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BTBD12 used at a dilution of 1:1,000 . Detection: DAB")
![Immunohistochemistry: BTBD12 Antibody [NBP2-39070] - Staining of human cervix, uterine shows moderate nucleolar positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/BTBD12-Antibody-Immunohistochemistry-NBP2-39070-img0001.jpg "Immunohistochemistry: BTBD12 Antibody [NBP2-39070] - Staining of human cervix, uterine shows moderate nucleolar positivity in squamous epithelial cells.")
![Immunocytochemistry/Immunofluorescence: BTBD12 Antibody [NBP2-58436] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/BTBD12-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58436-img0001.jpg "Immunocytochemistry/Immunofluorescence: BTBD12 Antibody [NBP2-58436] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol.")