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![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82884] - Nek1 expression in luminal cells of 12 weeks TRAMP mice prostate tissue. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82884-img0011.jpg "Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82884] - Nek1 expression in luminal cells of 12 weeks TRAMP mice prostate tissue. IHC-P image submitted by a verified customer review.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82884] - Analysis in human testis and skin tissues. Corresponding NEK1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82884-img0005.jpg)
![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82884] - Staining of human endometrium shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82884-img0006.jpg)
![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82884] - Staining of human Fallopian tube shows strong cytoplasmic/ membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82884-img0007.jpg)
![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82884] - Staining of human skin shows weak cytoplasmic positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82884-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82884] - Staining of human colon, lymph node, placenta and testis using Anti-GATAD2A antibody (A) NBP1-82884 shows similar protein distribution across tissues to independent antibody NBP1-82883 (B).](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82884-img0010.jpg)
![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82884] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82884-img0009.jpg)
![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Analysis in human testis and skin tissues using antibody. Corresponding NEK1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82883-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Analysis in human testis and skin tissues using antibody. Corresponding NEK1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Staining of human endometrium, fallopian tube, skin and testis using Anti-NEK1 antibody NBP1-82883 (A) shows similar protein distribution across tissues to independent antibody NBP1-82884 (B).](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82883-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: NEK1 Antibody [NBP1-82883] - Staining of human cell line U-251 MG shows localization to nuclear membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82883-img0006.jpg)
![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Staining of human Fallopian tube shows strong cytoplasmic/ membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82883-img0007.jpg)
![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Staining of human endometrium shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82883-img0008.jpg)
![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82883-img0009.jpg)
![Immunohistochemistry-Paraffin: NEK1 Antibody [NBP1-82883] - Staining of human skin shows weak cytoplasmic positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82883-img0010.jpg)
![Western Blot: NEK1 Antibody [NBP2-36534] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiNEK1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NEK1-Antibody-Western-Blot-NBP2-36534-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: NEK1 Antibody [NBP2-36534] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiNEK1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: NEK1 Antibody [NBP2-36534] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NEK1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/NEK1-Antibody-Western-Blot-NBP2-36534-img0002.jpg)
![Immunoprecipitation: NEK1 Antibody [NBP2-36533] - Detection of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NEK1 antibody NBP2-36533 used for IP at 6 ug per reaction. NEK1 was also immunoprecipitated by rabbit anti-NEK1 antibody NBP2-36534. For blotting immunoprecipitated NEK1, NBP2-36534 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/NEK1-Antibody-Immunoprecipitation-NBP2-36533-img0001.jpg "Immunoprecipitation: NEK1 Antibody [NBP2-36533] - Detection of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NEK1 antibody NBP2-36533 used for IP at 6 ug per reaction. NEK1 was also immunoprecipitated by rabbit anti-NEK1 antibody NBP2-36534. For blotting immunoprecipitated NEK1, NBP2-36534 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")