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![Western Blot: MBD2 Antibody [NB100-55415] - Staining of Jurkat cell lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MBD2-Antibody-Western-Blot-NB100-55415-img0002.jpg "Western Blot: MBD2 Antibody [NB100-55415] - Staining of Jurkat cell lysate (35 ug protein in RIPA buffer) with antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-55415] - Staining of paraffin embedded Human Skin with antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MBD2-Antibody-Immunohistochemistry-Paraffin-NB100-55415-img0007.jpg)
![Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-55415] - Staining of paraffin embedded Human Colon with antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MBD2-Antibody-Immunohistochemistry-Paraffin-NB100-55415-img0003.jpg)
![Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-55415] - Staining of paraffin embedded Human Adrenal Gland with antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MBD2-Antibody-Immunohistochemistry-Paraffin-NB100-55415-img0006.jpg)
![Western Blot: MBD2 Antibody [NB100-81657] - Detection of Human and Mouse MBD2 on HeLa whole cell lysate using NB100-81657. MBD2 was also immunoprecipitated by rabbit anti-MBD2 antibody NB100-81656.](http://images.novusbio.com/fullsize/MBD2-Antibody-Western-Blot-NB100-81657-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: MBD2 Antibody [NB100-81657] - Detection of Human and Mouse MBD2 on HeLa whole cell lysate using NB100-81657. MBD2 was also immunoprecipitated by rabbit anti-MBD2 antibody NB100-81656.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-81657] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MBD2 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MBD2-Antibody-Immunohistochemistry-NB100-81657-img0012.jpg)
![Western Blot: MBD2 Antibody (SR1478) [NBP3-21856] - Western blot analysis using NBP3-21856 in A375 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21856_rabbit-mbd2-mab-sr1478-5720231552186.jpg "Western Blot: MBD2 Antibody (SR1478) [NBP3-21856] - Western blot analysis using NBP3-21856 in A375 cell lysate.")
![Western Blot: MBD2 Antibody [NBP2-56565] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/MBD2-Antibody-Western-Blot-NBP2-56565-img0002.jpg "Western Blot: MBD2 Antibody [NBP2-56565] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: MBD2 Antibody [NBP2-56565] - Staining of human cell line HaCaT shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/MBD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56565-img0001.jpg)
![Western Blot: MBD2 Antibody (SR1726) [NBP3-22073] - Western blot analysis using NBP3-22073 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22073_rabbit-mbd2-mab-sr1726-5720231754851.jpg "Western Blot: MBD2 Antibody (SR1726) [NBP3-22073] - Western blot analysis using NBP3-22073 in A431 cell lysate.")
![Immunohistochemistry-Paraffin: MBD2 Antibody [NBP3-10941] - Immunohistochemical analysis of formalin-fixed paraffin-embedded human adult liver tissue.](http://images.novusbio.com/fullsize/MBD2-Antibody-Immunohistochemistry-Paraffin-NBP3-10941-img0001.jpg "Immunohistochemistry-Paraffin: MBD2 Antibody [NBP3-10941] - Immunohistochemical analysis of formalin-fixed paraffin-embedded human adult liver tissue.")
![Western Blot: MBD2 Antibody [NBP3-18666] - Figure 2. Western blot analysis using the antibody directed against MBD2 (1) Western blot was performed on nuclear extracts from mouse fibroblastst (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse MBD2 , diluted 1:500 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/MBD2-Antibody-Western-Blot-NBP3-18666-img0001.jpg "Western Blot: MBD2 Antibody [NBP3-18666] - Figure 2. Western blot analysis using the antibody directed against MBD2 (1) Western blot was performed on nuclear extracts from mouse fibroblastst (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse MBD2 , diluted 1:500 in BSA/PBS- Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest is indicated on the right.")
![ELISA: MBD2 Antibody [NBP3-18666] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse MBD2 . By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:185,000.](http://images.novusbio.com/fullsize/MBD2 Antibody-ELISA-NBP3-18666-img0002.jpg)
![Western Blot: MBD2 Antibody [NBP3-04835] - Analysis of extracts of various cell lines, using MBD2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/MBD2-Antibody-Western-Blot-NBP3-04835-img0001.jpg "Western Blot: MBD2 Antibody [NBP3-04835] - Analysis of extracts of various cell lines, using MBD2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/Immunofluorescence: MBD2 Antibody [NBP2-56638] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/MBD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56638-img0001.jpg "Immunocytochemistry/Immunofluorescence: MBD2 Antibody [NBP2-56638] - Staining of human cell line A-431 shows localization to nucleoplasm.")
![Western Blot: MBD2 Antibody [NBP3-18692] - Figure 1. Western blot analysis using the antibody directed against MBD2 Whole cell extracts (40 ug) from HeLa cells, transfected with a TY1-tagged MBD2 (lane 2) or an untransfected negative control (lane 1) were analysed by western blot using the antibody against MBD2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the TY1-tagged MBD2 protein is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/MBD2 Antibody-Western Blot-NBP3-18692-img0001.jpg "Western Blot: MBD2 Antibody [NBP3-18692] - Figure 1. Western blot analysis using the antibody directed against MBD2 Whole cell extracts (40 ug) from HeLa cells, transfected with a TY1-tagged MBD2 (lane 2) or an untransfected negative control (lane 1) were analysed by western blot using the antibody against MBD2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the TY1-tagged MBD2 protein is indicated on the right; the marker (in kDa) is shown on the left.")
![Western Blot: MBD2 Antibody [NBP2-99402] - Anti-MBD2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa](http://images.novusbio.com/fullsize/MBD2-Antibody-Western-Blot-NBP2-99402-img0002.jpg "Western Blot: MBD2 Antibody [NBP2-99402] - Anti-MBD2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 43 kDa")
![Western Blot: MBD2 Antibody (4D4D5) [NBP3-16317] - Western blot analysis of extracts of Mouse liver cells, using MBD2 antibody (NBP3-16317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MBD2-Antibody-4D4D5-Western-Blot-NBP3-16317-img0001.jpg "Western Blot: MBD2 Antibody (4D4D5) [NBP3-16317] - Western blot analysis of extracts of Mouse liver cells, using MBD2 antibody (NBP3-16317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")