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![Immunocytochemistry/Immunofluorescence: Blooms Syndrome Protein Blm Antibody [NBP1-89929] - Staining of human cell line A-431 shows positivity in nucleus and cytoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89929-img0003.jpg "Immunocytochemistry/Immunofluorescence: Blooms Syndrome Protein Blm Antibody [NBP1-89929] - Staining of human cell line A-431 shows positivity in nucleus and cytoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Blooms Syndrome Protein Blm Antibody [NBP1-89929] - Staining of human stomach shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Immunohistochemistry-Paraffin-NBP1-89929-img0002.jpg)
![Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (15 ug) from HEK293T, K-562, Jurkat, HeLa, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-214 (lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Western-Blot-NB100-214-img0003.jpg "Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (15 ug) from HEK293T, K-562, Jurkat, HeLa, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-214 (lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (50 ug) from TCMK-1 and BIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-214 (lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Western-Blot-NB100-214-img0002.jpg)
![Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody (NB100-214, lot 4) used for IP at 6 ug per reaction. BLM was also immunoprecipitated by a previous lot of this antibody (lot 3). For blotting immunoprecipitated BLM, NB100-214 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Immunoprecipitation-NB100-214-img0001.jpg)
![Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-1590] - Detection of Human and Mouse BLM by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-1590 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Western-Blot-NB100-1590-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-1590] - Detection of Human and Mouse BLM by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-1590 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-1590] - Whole cell lysate from mouse NIH3T3 cells. BLM was also immunoprecipitated using rabbit anti-BLM antibodies NB100-1588 and NB100-1589.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Western-Blot-NB100-1590-img0001.jpg)
![Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Western-Blot-NB100-324-img0003.jpg "Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-324] - Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-BLM antibody NB100-324 (lot NB100-324-2) used for IP at 6 ug per reaction. BLM was also immunoprecipitated by a previous lot of this antibody (lot NB100-324-1) and rabbit anti-BLM antibody NB100-214. For blotting immunoprecipitated BLM, NB100-324 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Immunoprecipitation-NB100-324-img0005.jpg)
![SDS-Page: Recombinant Human Blooms Syndrome Protein Blm Protein [H00000641-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Blooms-Syndrome-Protein-Blm-Protein-SDS-Page-H00000641-Q01-img0001.jpg "SDS-Page: Recombinant Human Blooms Syndrome Protein Blm Protein [H00000641-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")