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![Immunohistochemistry-Paraffin: Brevican Antibody [NBP1-89992] - Analysis in human cerebral cortex and pancreas tissues using NBP1-89992 antibody. Corresponding BCAN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Brevican-Antibody-Immunohistochemistry-Paraffin-NBP1-89992-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Brevican Antibody [NBP1-89992] - Analysis in human cerebral cortex and pancreas tissues using NBP1-89992 antibody. Corresponding BCAN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Brevican Antibody [NBP1-89992] - Staining of human Pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Brevican-Antibody-Immunohistochemistry-Paraffin-NBP1-89992-img0010.jpg)
![Immunohistochemistry-Paraffin: Brevican Antibody [NBP1-89992] - Staining of human Cerebral cortex shows strong positivity in neuronal processes in neuropil.](http://images.novusbio.com/fullsize/Brevican-Antibody-Immunohistochemistry-Paraffin-NBP1-89992-img0008.jpg)
![Immunohistochemistry-Paraffin: Brevican Antibody [NBP1-89992] - Staining of human Liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Brevican-Antibody-Immunohistochemistry-Paraffin-NBP1-89992-img0009.jpg)
![Immunohistochemistry-Paraffin: Brevican Antibody [NBP1-89992] - Staining of human Prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Brevican-Antibody-Immunohistochemistry-Paraffin-NBP1-89992-img0011.jpg)
![Western Blot: Brevican Antibody (S294A-6) [NBP2-22401] - Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~140 kDa.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Western-Blot-NBP2-22401-img0003.jpg "Western Blot: Brevican Antibody (S294A-6) [NBP2-22401] - Western Blot analysis of Rat Brain Membrane showing detection of ~140 kDa Brevican protein using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~140 kDa.")
![Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Brevican Antibody (D) Composite.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Immunocytochemistry-Immunofluorescence-NBP2-22401-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Immunocytochemistry-Immunofluorescence-NBP2-22401-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Brevican Antibody (S294A-6) [NBP2-22401] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Brevican Monoclonal Antibody, Clone S294A-6 (NBP2-22401). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Brevican Monoclonal Antibody (NBP2-22401) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Brevican Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Brevican-Antibody-S294A-6-Immunocytochemistry-Immunofluorescence-NBP2-22401-img0005.jpg)
![Western Blot: Brevican Antibody [NBP2-15616] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Brevican antibody [N1N2], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Brevican-Antibody-Western-Blot-NBP2-15616-img0004.jpg "Western Blot: Brevican Antibody [NBP2-15616] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Brevican antibody [N1N2], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Brevican Antibody [NBP2-15616] - Human breast cancer, using Brevican antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Brevican-Antibody-Immunohistochemistry-Paraffin-NBP2-15616-img0002.jpg)
![Western Blot: Brevican Antibody [H00063827-B01P] - Analysis of BCAN expression in transfected 293T cell line by BCAN polyclonal antibody. Lane 1: BCAN transfected lysate(100.21 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Brevican-Antibody-Western-Blot-H00063827-B01P-img0002.jpg "Western Blot: Brevican Antibody [H00063827-B01P] - Analysis of BCAN expression in transfected 293T cell line by BCAN polyclonal antibody. Lane 1: BCAN transfected lysate(100.21 KDa). Lane 2: Non-transfected lysate.")
![ELISA: Human Brevican ELISA Kit (Colorimetric) [NBP2-69940] - Samples were spiked with high concentrations of Human Brevican and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69940_human-brevican-elisa-kit-colorimetric-13220241620458.jpg "ELISA: Human Brevican ELISA Kit (Colorimetric) [NBP2-69940] - Samples were spiked with high concentrations of Human Brevican and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Brevican ELISA Kit (Colorimetric) [NBP2-69940] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69940_human-brevican-elisa-kit-colorimetric-432024903516.jpg)
![Western Blot: Brevican Overexpression Lysate (Adult Normal) [NBP2-06487] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Brevican.](http://images.novusbio.com/fullsize/Brevican-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06487-img0001.jpg "Western Blot: Brevican Overexpression Lysate (Adult Normal) [NBP2-06487] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Brevican.")