| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF222-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1477-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2221-SP | |||
| Carrier Free | FAB2221V-100UG | |||
| Carrier Free | FAB2221U-100UG | |||
| Carrier Free | FAB2221T-100UG | |||
| Carrier Free | FAB2221S-100UG | |||
| Carrier Free | FAB2221R-100UG | |||
| Carrier Free | FAB2221N-100UG | |||
| Carrier Free | FAB2221G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB222-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1477-SP | |||
![Western Blot: Activin RIB/ALK-4 Antibody (3U6I8) [NBP3-16106] - Western blot analysis of extracts of various cell lines, using Activin RIB/ALK-4 Rabbit mAb (NBP3-16106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Activin-RIB-ALK-4-Antibody-3U6I8-Western-Blot-NBP3-16106-img0005.jpg "Western Blot: Activin RIB/ALK-4 Antibody (3U6I8) [NBP3-16106] - Western blot analysis of extracts of various cell lines, using Activin RIB/ALK-4 Rabbit mAb (NBP3-16106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Activin RIB/ALK-4 Antibody (3U6I8) [NBP3-16106] - Immunohistochemistry of paraffin-embedded mouse brain using Activin RIB/ALK-4 Rabbit mAb (NBP3-16106) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Activin-RIB-ALK-4-Antibody-3U6I8-Immunohistochemistry-Paraffin-NBP3-16106-img0003.jpg)
![Immunohistochemistry-Paraffin: Activin RIB/ALK-4 Antibody (3U6I8) [NBP3-16106] - Immunohistochemistry of paraffin-embedded rat testis using Activin RIB/ALK-4 Rabbit mAb (NBP3-16106) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Activin-RIB-ALK-4-Antibody-3U6I8-Immunohistochemistry-Paraffin-NBP3-16106-img0001.jpg)
![Immunohistochemistry-Paraffin: Activin RIB/ALK-4 Antibody (3U6I8) [NBP3-16106] - Immunohistochemistry of paraffin-embedded human liver using Activin RIB/ALK-4 Rabbit mAb (NBP3-16106) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Activin-RIB-ALK-4-Antibody-3U6I8-Immunohistochemistry-Paraffin-NBP3-16106-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Activin RIB/ALK-4 Antibody (3U6I8) [NBP3-16106] -Confocal imaging of NIH/3T3 cells using ACVR1B Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red).The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16106_rabbit-activin-rib-alk-4-mab-3u6i8-immunocytochemistry-immunofluorescence-812024123048..jpg)
![Immunohistochemistry-Paraffin: Activin RIB/ALK-4 Antibody (3U6I8) [NBP3-16106] - Mouse testis tissue using ACVR1B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16106_rabbit-activin-rib-alk-4-mab-3u6i8-immunohistochemistry-paraffin-812024123213..jpg)
![Immunohistochemistry-Paraffin: Activin RIB/ALK-4 Antibody (3U6I8) [NBP3-16106] - Mouse kidney tissue using ACVR1B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16106_rabbit-activin-rib-alk-4-mab-3u6i8-immunohistochemistry-paraffin-812024123332..jpg)
![Immunohistochemistry-Paraffin: Activin RIB/ALK-4 Antibody (3U6I8) [NBP3-16106] - Human colon tissue using ACVR1B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16106_rabbit-activin-rib-alk-4-mab-3u6i8-immunohistochemistry-paraffin-812024123452..jpg)
![Immunohistochemistry: Activin RIB/ALK-4 Antibody (JE38-48) [NBP3-31998] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Activin RIB/ALK-4 antibody (NBP3-31998) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31998) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-31998_rabbit-activin-rib-alk-4-mab-je38-48-298202413585566.jpg "Immunohistochemistry: Activin RIB/ALK-4 Antibody (JE38-48) [NBP3-31998] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Activin RIB/ALK-4 antibody (NBP3-31998) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-31998) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Activin RIB/ALK-4 Antibody (JE38-48) [NBP3-31998] - Immunocytochemistry analysis of U-87 MG cells labeling Activin RIB/ALK-4 with Rabbit anti-Activin RIB/ALK-4 antibody (NBP3-31998) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Activin RIB/ALK-4 antibody (NBP3-31998) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-31998_rabbit-activin-rib-alk-4-mab-je38-48-29202410234236.jpg)
![Flow Cytometry: Activin RIB/ALK-4 Antibody (JE38-48) [NBP3-31998] - Flow cytometric analysis of U-87 MG cells labeling Activin RIB/ALK-4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-31998, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-31998_rabbit-activin-rib-alk-4-mab-je38-48-392024884652.jpg)
![Western Blot: Activin RIB/ALK-4 Antibody (JE38-48) [NBP3-31998] - Western blot analysis of Activin RIB/ALK-4 on different lysates with Rabbit anti-Activin RIB/ALK-4 antibody (NBP3-31998) at 1/1,000 dilution. Lane 1: 293T cell lysate (15 ug/Lane) Lane 2: U-87 MG cell lysate (20 ug/Lane) Lane 3: Mouse brain tissue lysate (20 ug/Lane) Lane 4: Rat brain tissue lysate (20 ug/Lane) Predicted band size: 57 kDa Observed band size: 52 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-31998) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-31998_rabbit-activin-rib-alk-4-mab-je38-48-3920248453516.jpg)
![Western Blot: Activin RIB/ALK-4 Antibody [NBP2-39003] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/Activin-RIB-ALK-4-Antibody-Western-Blot-NBP2-39003-img0002.jpg "Western Blot: Activin RIB/ALK-4 Antibody [NBP2-39003] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue")
![Immunocytochemistry/Immunofluorescence: Activin RIB/ALK-4 Antibody [NBP2-39003] - Immunofluorescent staining of human cell line A549 shows localization to cytosol.](http://images.novusbio.com/fullsize/Activin-RIB-ALK-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-39003-img0003.jpg)
![Immunohistochemistry-Paraffin: Activin RIB/ALK-4 Antibody [NBP2-39003] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Activin-RIB-ALK-4-Antibody-Immunohistochemistry-Paraffin-NBP2-39003-img0004.jpg)
![Western Blot: Activin RIB/ALK-4 Antibody (S08-8D6) [NBP3-19558] - Western blot detection of Activin RIB/ALK-4 in CHO-K1 (Lane 1), Rat Brain (Lane 2) lysates using NBP3-19558 (1:500 diluted). Predicted band size: 57kDa. Observed band size: 57kDa.](http://images.novusbio.com/fullsize/Activin-RIB-ALK-4-Antibody-S08-8D6-Western-Blot-NBP3-19558-img0002.jpg "Western Blot: Activin RIB/ALK-4 Antibody (S08-8D6) [NBP3-19558] - Western blot detection of Activin RIB/ALK-4 in CHO-K1 (Lane 1), Rat Brain (Lane 2) lysates using NBP3-19558 (1:500 diluted). Predicted band size: 57kDa. Observed band size: 57kDa.")
![Immunocytochemistry/Immunofluorescence: Activin RIB/ALK-4 Antibody (S08-8D6) [NBP3-19558] - Immunofluorescence of Activin RIB/ALK-4 (green) in hela using NBP3-19558 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/Activin-RIB-ALK-4-Antibody-S08-8D6-Immunocytochemistry-Immunofluorescence-NBP3-19558-img0001.jpg)
![Western Blot: Activin RIB/ALK-4 Antibody (S07-7H9) [NBP3-19557] - Western blot detection of Activin RIB/ALK-4 in K562 cell lysates using NBP3-19557 (1:1000 diluted). Predicted band size: 57kDa. Observed band size: 57kDa.](http://images.novusbio.com/fullsize/Activin-RIB-ALK-4-Antibody-S07-7H9-Western-Blot-NBP3-19557-img0001.jpg "Western Blot: Activin RIB/ALK-4 Antibody (S07-7H9) [NBP3-19557] - Western blot detection of Activin RIB/ALK-4 in K562 cell lysates using NBP3-19557 (1:1000 diluted). Predicted band size: 57kDa. Observed band size: 57kDa.")