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![Western Blot: FXR/NR1H4 Antibody [NB400-153] - Total protein from human A549, U2OS cell and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FXR (NB400-153) in block buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-Western-Blot-NB400-153-img0006.jpg "Western Blot: FXR/NR1H4 Antibody [NB400-153] - Total protein from human A549, U2OS cell and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FXR (NB400-153) in block buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB400-153] - A549 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-FXR/NR1H4 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-Immunocytochemistry-Immunofluorescence-NB400-153-img0005.jpg)
![Western Blot: FXR Antibody [NB400-153] - Detection of FXR in HeLa nuclear extract using NB400-153. ECL 30 minute detection.](http://images.novusbio.com/fullsize/FXR-Antibody-Western-Blot-NB400-153-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB400-153] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-FXR/NR1H4 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-Immunocytochemistry-Immunofluorescence-NB400-153-img0004.jpg)
![Western Blot: FXR/NR1H4 Antibody [NBP2-16550] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NR1H4 antibody.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-Western-Blot-NBP2-16550-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: FXR/NR1H4 Antibody [NBP2-16550] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NR1H4 antibody.")
![Western Blot: FXR/NR1H4 Antibody [NBP2-16550] -Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NR1H4 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16550_rabbit-polyclonal-fxr-nr1h4-antibody-western-blot-1220249182..jpg)
![Western Blot: FXR/NR1H4 Antibody [NBP2-16550] - Biochemical measurements & the mRNA & protein expression of top 5 NRs. A, Results of biochemical parameter measurements. Among the serum indicators, ALT in HFD group higher than it in NC group (P = .0004) & CSP group (P = .0222); the AST in HFD group higher than it in NC group (P = .0006) & CSP group (P = .0149); ALP in HFD group higher than it in NC group (P < .0001) & CSP group (P = .0013), & it lower in NC group than in CSP group (P = .0017); in HFD group, the TC level higher than it in NC group (P < .0001) & CSP group (P = .0003), & in NC group, it lower than it in CSP group (P < .0001); in HFD group, the TG level higher than it in NC group (P = .0011) & CSP group (P = .0476); in HFD group, the GLU level higher than it in NC group (P < .0001) & CSP group (P = .0002); & in HFD group, the HDL‐c level higher than it in NC group (P < .0001) & CSP group (P = .0022), & in NC group, it lower than it in CSP group (P < .0001) (n = 10). Among the liver indicators, in NC group, the TC level lower than it in HFD group (P = .0276) & CSP group (P = .0427); in NC group, the TG level lower than it in HFD group (P = .0427) & CSP group (P = .0448) (n = 6). B, The mRNA expression of top 5 NRs in liver. PPAR gamma in HFD group expressed at a higher level than it in NC group (P = .0072) & CSP group (P = .0489); FXR in HFD group expressed at a lower level than it in NC group (P = .0222) & CSP group (P = .0331); PPAR alpha in HFD group expressed lower than NC group (P = .0143) & CSP group (P = .0082); & RAR alpha in HFD group expressed at a higher level than it in NC group (P = .0001) & CSP group (P = .0012) (n = 10). C, Expression of top 5 NRs in liver measured by automatic WB analysis Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32189432), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16550_rabbit-polyclonal-fxr-nr1h4-antibody-310202416113545.jpg)
![Western Blot: FXR/NR1H4 Antibody (1B10) [H00009971-M02] - NR1H4 monoclonal antibody (M02), clone 1B10 Analysis of NR1H4 expression in HepG2.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-1B10-Western-Blot-H00009971-M02-img0007.jpg "Western Blot: FXR/NR1H4 Antibody (1B10) [H00009971-M02] - NR1H4 monoclonal antibody (M02), clone 1B10 Analysis of NR1H4 expression in HepG2.")
![Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody (1B10) [H00009971-M02] - Analysis of monoclonal antibody to NR1H4 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-1B10-Immunocytochemistry-Immunofluorescence-H00009971-M02-img0005.jpg)
![ELISA: FXR/NR1H4 Antibody (1B10) [H00009971-M02] - Detection limit for recombinant GST tagged NR1H4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-1B10-ELISA-H00009971-M02-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB100-55416] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-55416_goat-polyclonal-fxr-nr1h4-antibody-16520239183329.jpg "Immunocytochemistry/Immunofluorescence: FXR/NR1H4 Antibody [NB100-55416] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Flow Cytometry: FXR/NR1H4 Antibody [NB100-55416] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb100-55416_goat-polyclonal-fxr-nr1h4-antibody-16520239183323.jpg)
![Western Blot: FXR/NR1H4 Antibody (4F12) [NBP2-46221] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1H4 (RC217443, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1H4 (1:2000).](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-4F12-Western-Blot-NBP2-46221-img0004.jpg "Western Blot: FXR/NR1H4 Antibody (4F12) [NBP2-46221] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1H4 (RC217443, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1H4 (1:2000).")
![Immunohistochemistry-Paraffin: FXR/NR1H4 Antibody (4F12) [NBP2-46221] - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NR1H4 mouse monoclonal antibody (1:150). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-4F12-Immunohistochemistry-Paraffin-NBP2-46221-img0003.jpg)
![Western Blot: FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free [NBP2-71526] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1H4 (RC217443, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1H4](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-OTI4F12-Azide-and-BSA-Free-Western-Blot-NBP2-71526-img0001.jpg "Western Blot: FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free [NBP2-71526] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1H4 (RC217443, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1H4")
![Immunohistochemistry: FXR/NR1H4 Antibody (OTI4F12) - Azide and BSA Free [NBP2-71526] - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NR1H4 mouse monoclonal antibody (1:150). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min.](http://images.novusbio.com/fullsize/FXR-NR1H4-Antibody-OTI4F12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71526-img0002.jpg)
![Western Blot: FXR Overexpression Lysate (Adult Normal) [NBL1-13769] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FXR.](http://images.novusbio.com/fullsize/FXR-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13769-img0002.jpg "Western Blot: FXR Overexpression Lysate (Adult Normal) [NBL1-13769] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FXR.")
![ELISA: Human FXR/NR1H4 ELISA Kit (Colorimetric) [NBP3-31581] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31581_human-fxr-nr1h4-elisa-kit-colorimetric-20620241533935.png "ELISA: Human FXR/NR1H4 ELISA Kit (Colorimetric) [NBP3-31581] - Standard Curve Reference")