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![Immunohistochemistry-Paraffin: MDA5 Antibody [NB110-1244] - Staining in paraffin embedded Human Pancreas. Antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunohistochemistry-Paraffin-NB110-1244-img0001.jpg "Immunohistochemistry-Paraffin: MDA5 Antibody [NB110-1244] - Staining in paraffin embedded Human Pancreas. Antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.")
![Flow Cytometry: MDA5 Antibody [NB110-1244] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/MDA5-Antibody-Flow-Cytometry-NB110-1244-img0002.jpg)
![Western Blot: MDA5 Antibody [NBP1-76760] - Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.](http://images.novusbio.com/fullsize/MDA5-Antibody-Western-Blot-NBP1-76760-img0001.jpg "Western Blot: MDA5 Antibody [NBP1-76760] - Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.")
![Immunocytochemistry/Immunofluorescence: MDA5 Antibody [NBP1-76760] - Human Lymph Node cells.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76760-img0003.jpg)
![Immunohistochemistry-Paraffin: MDA5 Antibody [NBP1-76760] - Analysis of MDA5 in human lymph node tissue with MDA5 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunohistochemistry-Paraffin-NBP1-76760-img0004.jpg)
![Immunohistochemistry-Paraffin: MDA5 Antibody [NBP1-76760] - Human lymph node tissue with MDA5 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunohistochemistry-Paraffin-NBP1-76760-img0002.jpg)
![Western Blot: MDA5 Antibody [NBP1-76761] - Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.](http://images.novusbio.com/fullsize/MDA5-Antibody-Western-Blot-NBP1-76761-img0001.jpg "Western Blot: MDA5 Antibody [NBP1-76761] - Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.")
![Immunocytochemistry/Immunofluorescence: MDA5 Antibody [NBP1-76761] - Immunofluorescence of MDA5 in Human Lymph Node cells with MDA5 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76761-img0003.jpg)
![Immunohistochemistry-Paraffin: MDA5 Antibody [NBP1-76761] - Human lymph node tissue with MDA5 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunohistochemistry-Paraffin-NBP1-76761-img0002.jpg)
![Western Blot: MDA5 Antibody [NBP1-89685] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: SK-MEL-30](http://images.novusbio.com/fullsize/MDA5-Antibody-Western-Blot-NBP1-89685-img0005.jpg "Western Blot: MDA5 Antibody [NBP1-89685] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: SK-MEL-30")
![Immunohistochemistry-Paraffin: MDA5 Antibody [NBP1-89685] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89685-img0004.jpg)
![Immunohistochemistry-Paraffin: MDA5 Antibody [NBP2-17243] - Human breast carcinoma.](http://images.novusbio.com/fullsize/MDA5-Antibody-Immunohistochemistry-Paraffin-NBP2-17243-img0002.jpg "Immunohistochemistry-Paraffin: MDA5 Antibody [NBP2-17243] - Human breast carcinoma.")
![<b>Biological Strategies Validation. </b>Western Blot: MDA5 Antibody [NBP2-17243] - Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDA5 antibody [N2C1], Internal (NBP2-17243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17243_rabbit-polyclonal-mda5-antibody-910202420134057.jpg)
![Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132] - Analysis of extracts of various cell lines, using MDA5 antibody (NBP3-16132) at 1:1000 dilution.C6 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/MDA5-Antibody-2N8Z5-Western-Blot-NBP3-16132-img0001.jpg "Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132] - Analysis of extracts of various cell lines, using MDA5 antibody (NBP3-16132) at 1:1000 dilution.C6 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: MDA5 Antibody (2N8Z5) [NBP3-16132] - Analysis of extracts of BxPC-3 cells, using MDA5 antibody (NBP3-16132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MDA5-Antibody-2N8Z5-Western-Blot-NBP3-16132-img0002.jpg)
![Immunohistochemistry-Paraffin: MDA5 Antibody (3F6) [H00064135-M02] - Analysis of monoclonal antibody to IFIH1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MDA5-Antibody-3F6-Immunohistochemistry-Paraffin-H00064135-M02-img0002.jpg "Immunohistochemistry-Paraffin: MDA5 Antibody (3F6) [H00064135-M02] - Analysis of monoclonal antibody to IFIH1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.")
![Sandwich ELISA: MDA5 Antibody (3F6) [H00064135-M02] - Detection limit for recombinant GST tagged IFIH1 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MDA5-Antibody-3F6-Sandwich-ELISA-H00064135-M02-img0003.jpg)
![Western Blot: MDA5 Antibody (SR1076) [NBP3-21840] - Western blot analysis using NBP3-21840 in THP1 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21840_rabbit-mda5-mab-sr1076-57202316112145.jpg "Western Blot: MDA5 Antibody (SR1076) [NBP3-21840] - Western blot analysis using NBP3-21840 in THP1 cell lysate.")
![SDS-Page: Recombinant Human MDA5 Protein [H00064135-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00064135-Q01-1.jpg "SDS-Page: Recombinant Human MDA5 Protein [H00064135-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: MDA5 Antibody (PSH03-56) [NBP3-32581] - Western blot analysis of MDA5 on different lysates with Rabbit anti-MDA5 antibody (NBP3-32581) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: Daudi cell lysate (20 ug/Lane) Lane 5: Raji cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: HeLa cell lysate (20 ug/Lane) Lane 8: SH-SY5Y cell lysate (20 ug/Lane) Lane 9: A549 cell lysate (20 ug/Lane) Lane 10: A431 cell lysate (20 ug/Lane) Lane 11: Mouse pancreas tissue lysate (40 ug/Lane) Lane 12: Rat kidney tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 117 kDa Observed band size: 140/100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32581) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32581_rabbit-mda5-mab-psh03-56-278202415295575.jpg "Western Blot: MDA5 Antibody (PSH03-56) [NBP3-32581] - Western blot analysis of MDA5 on different lysates with Rabbit anti-MDA5 antibody (NBP3-32581) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: Daudi cell lysate (20 ug/Lane) Lane 5: Raji cell lysate (20 ug/Lane) Lane 6: MCF7 cell lysate (20 ug/Lane) Lane 7: HeLa cell lysate (20 ug/Lane) Lane 8: SH-SY5Y cell lysate (20 ug/Lane) Lane 9: A549 cell lysate (20 ug/Lane) Lane 10: A431 cell lysate (20 ug/Lane) Lane 11: Mouse pancreas tissue lysate (40 ug/Lane) Lane 12: Rat kidney tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 117 kDa Observed band size: 140/100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32581) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: MDA5 Antibody (1B6) [NBP1-97526] - Analysis of MDA5 monoclonal antibody (1B6) : Lane 1: MW marker, Lane 2: HeLa, Lane 3: A431, Lane 4: L929, Lane 5: 3T3, Lane 6: Jurkat cell lysate.](http://images.novusbio.com/fullsize/MDA5-Antibody-1B6-Western-Blot-NBP1-97526-img0001.jpg "Western Blot: MDA5 Antibody (1B6) [NBP1-97526] - Analysis of MDA5 monoclonal antibody (1B6) : Lane 1: MW marker, Lane 2: HeLa, Lane 3: A431, Lane 4: L929, Lane 5: 3T3, Lane 6: Jurkat cell lysate.")