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MDA5 Antibody (3F6)

Images

 
Immunohistochemistry-Paraffin: MDA5 Antibody (3F6) [H00064135-M02] - Analysis of monoclonal antibody to IFIH1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
Sandwich ELISA: MDA5 Antibody (3F6) [H00064135-M02] - Detection limit for recombinant GST tagged IFIH1 is 3 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC
Clone
3F6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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MDA5 Antibody (3F6) Summary

Immunogen
IFIH1 (NP_071451.2, 928 a.a. ~ 1023 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HVNMTPEFKELYIVRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQYKKWVELPITFPNLDYSECCLFSD
Specificity
IFIH1 - interferon induced with helicase C domain 1 (3F6)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IFIH1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
Application Notes
This product is useful for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MDA5 Antibody (3F6)

  • AGS7
  • CADM-140 autoantigen
  • Clinically amyopathic dermatomyositis autoantigen 140 kDa
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
  • EC 3.6.4.13
  • Helicard
  • Helicase with 2 CARD domains
  • Hlcd
  • IDDM19
  • IFIH1
  • IMD95
  • interferon induced with helicase C domain 1
  • interferon-induced helicase C domain-containing protein 1
  • Interferon-induced with helicase C domain protein 1
  • MDA5
  • MDA-5
  • MDA-5MGC133047
  • MDA5RNA helicase-DEAD box protein 116
  • melanoma differentiation associated protein-5
  • Melanoma differentiation-associated protein 5
  • Murabutide down-regulated protein
  • RH116
  • SGMRT1

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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MDA5 - Part of the RIG-I-like Receptor Family
The innate immune system is responsible for reacting to viral infections through recognition of various viral components. Like toll-like receptor 3 (TLR3), MDA5 recognizes double-stranded (ds) RNA which is a molecular pattern indicative of viral infec...  Read full blog post.

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Bioinformatics

Gene Symbol IFIH1
Entrez
Uniprot