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![Immunocytochemistry/Immunofluorescence: PRDM14 Antibody [NBP1-72055] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/PRDM14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-72055-img0001.jpg "Immunocytochemistry/Immunofluorescence: PRDM14 Antibody [NBP1-72055] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: PRDM14 Antibody [NBP1-72055] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PRDM14-Antibody-Flow-Cytometry-NBP1-72055-img0002.jpg)
![Western Blot: PRDM14 Antibody (1359) [NBP2-43593] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE PRDM14 antibody [1359] dilution: 1:1000](http://images.novusbio.com/fullsize/PRDM14-Antibody-1359-Western-Blot-NBP2-43593-img0002.jpg "Western Blot: PRDM14 Antibody (1359) [NBP2-43593] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE PRDM14 antibody [1359] dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: PRDM14 Antibody (1359) [NBP2-43593] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRDM14 protein stained by PRDM14 antibody [1359] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PRDM14-Antibody-1359-Immunocytochemistry-Immunofluorescence-NBP2-43593-img0001.jpg)
![Immunohistochemistry-Paraffin: PRDM14 Antibody [NBP1-86437] - Staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/PRDM14-Antibody-Immunohistochemistry-Paraffin-NBP1-86437-img0004.jpg "Immunohistochemistry-Paraffin: PRDM14 Antibody [NBP1-86437] - Staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.")
![Immunocytochemistry/Immunofluorescence: PRDM14 Antibody [NBP2-55342] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PRDM14-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55342-img0001.jpg "Immunocytochemistry/Immunofluorescence: PRDM14 Antibody [NBP2-55342] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Western Blot: PRDM14 Overexpression Lysate (Adult Normal) [NBP2-04395] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PRDM14.](http://images.novusbio.com/fullsize/PRDM14-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04395-img0001.jpg "Western Blot: PRDM14 Overexpression Lysate (Adult Normal) [NBP2-04395] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PRDM14.")
![Western Blot: PRDM14 Antibody [NBP2-98845] - Anti-PRDM14 rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 64 kDa. Observed band size: 84 kDa](http://images.novusbio.com/fullsize/PRDM14-Antibody-Western-Blot-NBP2-98845-img0001.jpg "Western Blot: PRDM14 Antibody [NBP2-98845] - Anti-PRDM14 rabbit polyclonal antibody at 1:500 dilution. Lane A: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 64 kDa. Observed band size: 84 kDa")