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![Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of HEK293 cell lysates using Adenylate Cyclase 3 antibody, dilution 1:2000 (Green): [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human Adenylate Cyclase 3 cDNA. The strong band at about 130kDa in the transfected cells demonstrates overexpression of the human Adenylate Cyclase 3 protein, and the bands over 250kDa presumably correspond to either glycosylated or aggregated forms of Adenylate Cyclase 3. The blot was simultaneously probed with mouse GAPDH mAb, dilution 1:5000 (Red), which reveals the single band at ~37kDa in both transfected and non-transfected cells.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP1-92683-img0005.jpg "Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of HEK293 cell lysates using Adenylate Cyclase 3 antibody, dilution 1:2000 (Green): [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human Adenylate Cyclase 3 cDNA. The strong band at about 130kDa in the transfected cells demonstrates overexpression of the human Adenylate Cyclase 3 protein, and the bands over 250kDa presumably correspond to either glycosylated or aggregated forms of Adenylate Cyclase 3. The blot was simultaneously probed with mouse GAPDH mAb, dilution 1:5000 (Red), which reveals the single band at ~37kDa in both transfected and non-transfected cells.")
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of rat hippocampus stained with NBP1-92683 antibody to adenylate cyclase III in red and mouse antibody to MECP2 in green, DNA is revealed with DAPI in blue. This is a stacked confocal image made with a 100X objective lens. The ACIII antibody reveals neuronal cilia while the MECP2 antibody reveals the nuclei of certain neurons to a variable degree.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92683-img0004.jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Western blot of rat olfactory epithelium (OE) and frontal cortex (FC). NBP1-92683 stains a band at about 200kDa in olfactory epithelium which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is less heavily glycosylated, and a less prominent band is seen at about 160kDa. Data generated in the laboratory of Matt Sarkisian in the University of Florida.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP1-92683-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP1-92683] - Mixed neuron-glial cultures stained with Adenylate Cyclase 3 antibody (NBP1-92683, red) and Alpha Fodrin antibody (NBP1-92689, green). Note the strong and clean staining of neuronal cilia. Since Alpha Fodrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92683-img0003.jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP3-05526] - Western blot analysis of HEK293 cell lysates using Adenylate Cyclase 3 Antibody, dilution 1:1,000, in green: [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with a vector encoding Myc-DDK tagged fill length human adenylate cyclase 3. The strong band at about 130kDa in transfected cells demonstrates overexpression of the human adenylate cyclase 3 protein, and bands over 250kDa presumably correspond to either heavily glycosolated form of adenylate cyclase 3 or multimeric forms of the protein. The same blot was simultaneously probed with rabbit polyclonal antibody to GAPDH dilution 1:20,000 in red, which reveals the single band at ~37kDa seen in both transfected and non-transfected cells.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP3-05526-img0002.jpg "Western Blot: Adenylate Cyclase 3 Antibody [NBP3-05526] - Western blot analysis of HEK293 cell lysates using Adenylate Cyclase 3 Antibody, dilution 1:1,000, in green: [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with a vector encoding Myc-DDK tagged fill length human adenylate cyclase 3. The strong band at about 130kDa in transfected cells demonstrates overexpression of the human adenylate cyclase 3 protein, and bands over 250kDa presumably correspond to either heavily glycosolated form of adenylate cyclase 3 or multimeric forms of the protein. The same blot was simultaneously probed with rabbit polyclonal antibody to GAPDH dilution 1:20,000 in red, which reveals the single band at ~37kDa seen in both transfected and non-transfected cells.")
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP3-05526] - Immunofluoresence of caudate/putamen region of rat brain stained with Adenylate Cyclase 3 Antibody in red, and chicken antibody to tyrosine hydroxylase, in green. The Blue is Hoechst stain revealing nuclei. The Adenylate Cyclase 3 Antibody reveals neuronal cilia while the tyrosine hydroxylase antibody reveals the axons of chatecholaminergic neurons.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05526-img0001.jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP3-05527] - used at a dilution of 1:1,000, in green. [1] protein standard, [2] rat hippocampus, [3] mouse hippocampus, [4] mouse frontal cortex, and [5] cow frontal cortex, [6] protein standard, [7] non-transfected HEK293 cells, and [8] HEK293 cells transfected with DNA expressing Myc-DDK tagged full length human Adenylate Cyclase 3 from the appropriate cDNA. This antibody detects variably glycosylated forms of Adenylate Cyclase 3 protein with apparent molecular weights from ~120kDa and higher. The strong band at about 130kDa demonstrates overexpression of the Adenylate Cyclase 3 protein, and those over 250kDA double band presumably corresponds to heavily glycosolated or aggregated forms of Adenylate Cyclase 3. The same blot was simultaneously probed with mouse monoclonal antibody to GAPDH at a at a dilution of of 1:5,000, in red, which reveals the single GAPDH band at ~37kDa in both transfected and non-transfected cells.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP3-05527-img0002.jpg "Western Blot: Adenylate Cyclase 3 Antibody [NBP3-05527] - used at a dilution of 1:1,000, in green. [1] protein standard, [2] rat hippocampus, [3] mouse hippocampus, [4] mouse frontal cortex, and [5] cow frontal cortex, [6] protein standard, [7] non-transfected HEK293 cells, and [8] HEK293 cells transfected with DNA expressing Myc-DDK tagged full length human Adenylate Cyclase 3 from the appropriate cDNA. This antibody detects variably glycosylated forms of Adenylate Cyclase 3 protein with apparent molecular weights from ~120kDa and higher. The strong band at about 130kDa demonstrates overexpression of the Adenylate Cyclase 3 protein, and those over 250kDA double band presumably corresponds to heavily glycosolated or aggregated forms of Adenylate Cyclase 3. The same blot was simultaneously probed with mouse monoclonal antibody to GAPDH at a at a dilution of of 1:5,000, in red, which reveals the single GAPDH band at ~37kDa in both transfected and non-transfected cells.")
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP3-05527] - Immunofluorescence analysis of rat cortex section stained with Adenylate Cyclase 3 Antibody at a at a dilution of of 1:10,000, in red and costained with mouse monoclonal antibody to the myelin and oligodendrocyte marker CNP at a at a dilution of of 1:1,000 in green. The blue is Hoechst staining of nuclear DNA. The Adenylate Cyclase 3 Antibody reveals neuronal cilia while the CNP antibody stains oligodendrocytes and the myelin sheath around axons.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05527-img0001.jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP2-60792] - Western blot of rat cortical lysate showing specificimmunolabeling of the ~160 kDa adenylate cyclase III protein.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP2-60792-img0001.jpg "Western Blot: Adenylate Cyclase 3 Antibody [NBP2-60792] - Western blot of rat cortical lysate showing specificimmunolabeling of the ~160 kDa adenylate cyclase III protein.")
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP2-60792] - Immunofluorescence of a section of rat hippocampus selectively labeling the neuronal cilia with anti-adenylate cyclase III (1:10,000, red), labeling nuclei with anti-MECP2 (1:1000,green), and Hoechst staining of nuclear DNA.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60792-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP2-60792] - Immunofluorescence of cultured rat neurons and glia showingstrong staining of neuronal cilia using our anti-adenylatecyclase III antibody (red) and axonal and dendritic staining ofalpha II spectrin (green) revealing thesubmembraneous cytoskeleton and DNA (blue).](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-60792-img0002.jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP3-12369] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 129 KDa.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP3-12369-img0002.jpg "Western Blot: Adenylate Cyclase 3 Antibody [NBP3-12369] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 129 KDa.")
![Immunohistochemistry-Paraffin: Adenylate Cyclase 3 Antibody [NBP3-12369] - Mouse liver. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunohistochemistry-Paraffin-NBP3-12369-img0001.jpg)
![SDS-Page: Adenylate Cyclase 3 Partial Recombinant Protein [H00000109-Q01]](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Partial-Recombinant-Protein-SDS-Page-H00000109-Q01-img0001.jpg "SDS-Page: Adenylate Cyclase 3 Partial Recombinant Protein [H00000109-Q01]")
![Western Blot: Adenylate Cyclase 3 Antibody [NBP2-92522] - Analysis of extracts of various cell lines, using Adenylate Cyclase 3 .Exposure time: 60s.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP2-92522-img0001.jpg "Western Blot: Adenylate Cyclase 3 Antibody [NBP2-92522] - Analysis of extracts of various cell lines, using Adenylate Cyclase 3 .Exposure time: 60s.")
![ELISA: Human Adenylate Cyclase 3 ELISA Kit (Colorimetric) [NBP2-66450] - Samples were spiked with high concentrations of Human Adenylate Cyclase 3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66450_human-adenylate-cyclase-3-elisa-kit-colorimetric-13220241626143.jpg "ELISA: Human Adenylate Cyclase 3 ELISA Kit (Colorimetric) [NBP2-66450] - Samples were spiked with high concentrations of Human Adenylate Cyclase 3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Adenylate Cyclase 3 ELISA Kit (Colorimetric) [NBP2-66450] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66450_human-adenylate-cyclase-3-elisa-kit-colorimetric-43202492403.jpg)
![Western Blot: Adenylate Cyclase 3 Overexpression Lysate (Adult Normal) [NBP2-10559] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adenylate Cyclase 3.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10559-img0001.jpg "Western Blot: Adenylate Cyclase 3 Overexpression Lysate (Adult Normal) [NBP2-10559] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adenylate Cyclase 3.")