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![Western Blot: HCN4 Antibody (S114/10) [NBP2-12910] - Western Blot analysis of Human T-HEK cell lysate showing detection of HCN4 protein using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (NBP2-12910). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (NBP2-12910) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN4-Antibody-S114-10-Western-Blot-NBP2-12910-img0007.jpg "Western Blot: HCN4 Antibody (S114/10) [NBP2-12910] - Western Blot analysis of Human T-HEK cell lysate showing detection of HCN4 protein using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (NBP2-12910). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (NBP2-12910) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: HCN4 Antibody (S114/10) [NBP2-12910] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (NBP2-12910). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (NBP2-12910) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN4 Antibody (D) Composite.](http://images.novusbio.com/fullsize/HCN4-Antibody-S114-10-Immunocytochemistry-Immunofluorescence-NBP2-12910-img0009.jpg)
![Immunohistochemistry: HCN4 Antibody (S114/10) [NBP2-12910] - Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (NBP2-12910). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (NBP2-12910) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN4-Antibody-S114-10-Immunohistochemistry-NBP2-12910-img0008.jpg)
![Immunohistochemistry-Frozen: HCN4 Antibody (S114/10) [NBP2-12910] - Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 uL for 5 minutes at RT.](http://images.novusbio.com/fullsize/HCN4-Antibody-S114-10-Immunohistochemistry-Frozen-NBP2-12910-img0005.jpg)
![Western Blot: HCN4 Antibody (SHG 1E5) [NB100-74439] - Analysis of 25 ug of rat eye lysate onto an SDS polyacrylamide gel.](http://images.novusbio.com/fullsize/HCN4-Antibody-SHG-1E5-Western-Blot-NB100-74439-img0005.jpg "Western Blot: HCN4 Antibody (SHG 1E5) [NB100-74439] - Analysis of 25 ug of rat eye lysate onto an SDS polyacrylamide gel.")
![Immunohistochemistry-Paraffin: HCN4 Antibody (SHG 1E5) [NB100-74439] - Both normal and cancer biopsies of deparaffinized Human heart tissue tissues.](http://images.novusbio.com/fullsize/HCN4-Antibody-SHG-1E5-Immunohistochemistry-Paraffin-NB100-74439-img0004.jpg)
![Immunohistochemistry-Paraffin: HCN4 Antibody (SHG 1E5) [NB100-74439] - Both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues.](http://images.novusbio.com/fullsize/HCN4-Antibody-SHG-1E5-Immunohistochemistry-Paraffin-NB100-74439-img0003.jpg)
![Immunohistochemistry-Paraffin: HCN4 Antibody [NBP3-17864] - Staining of human caudate shows strong cytoplasmic positivity in neuronal cells and projections.](http://images.novusbio.com/fullsize/HCN4-Antibody-Immunohistochemistry-Paraffin-NBP3-17864-img0001.jpg "Immunohistochemistry-Paraffin: HCN4 Antibody [NBP3-17864] - Staining of human caudate shows strong cytoplasmic positivity in neuronal cells and projections.")
![Sandwich ELISA: HCN4 Antibody (2A5) [H00010021-M04] - Detection limit for recombinant GST tagged HCN4 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HCN4-Antibody-2A5-Sandwich-ELISA-H00010021-M04-img0001.jpg "Sandwich ELISA: HCN4 Antibody (2A5) [H00010021-M04] - Detection limit for recombinant GST tagged HCN4 is 0.1 ng/ml as a capture antibody.")