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73 Results for "cd25 il 2 r alpha"
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DR2A00

Human CD25/IL-2R alpha Quantikine ELISA Kit

(36 Publications)
1 Kit
DR2A00
N/A CD25/IL-2R alpha [HRP]
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Conjugate Catalog # Availability Size Price
HRP SR2A00
HRP PDR2A00
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MAB223

CD25/IL-2R alpha Antibody (22722) [Unconjugated]

(9 Publications)
100 μg
MAB223
CD25/IL-2 Ra was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA and monensin using Mouse Anti-Human CD25/IL-2 Ra Monoclonal Antibody (Catalog # MAB223) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Hu
Species Glossary
Reactivity: Hu
WB, B/N, ICC/IF
Applications: WB, B/N, ICC/IF
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB223-SP
Carrier Free MAB223-500
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MAB623

CD25/IL-2R alpha Antibody (24204) [Unconjugated]

(6 Publications)
100 μg
MAB623
Western blot shows 25 ng of Recombinant Human CD25/IL-2 R alpha (Catalog # 223-2A), Recombinant Mouse CD25/IL-2 R alpha (Catalog # 2438-RM) and Recombinant Rat CD25/IL-2 R alpha Fc Chimera (Catalog # 5156-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CD25/IL-2 R alpha Monoclonal Antibody (Catalog # MAB623) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD25/IL-2 R alpha at approximately 30-250 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3.
Hu
Species Glossary
Reactivity: Hu
WB, IHC, ELISA(Cap), ICC/IF
Applications: WB, IHC, ELISA(Cap), ICC/IF
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB623-SP
Carrier Free MAB623-500
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AF-223-NA

CD25/IL-2R alpha Antibody [Unconjugated]

(6 Publications)
100 μg
AF-223-NA
Western blot shows lysates of HDLM-2 human Hodgkin's lymphoma cell line and PC-3 human prostate cancer cell line (negative control). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD25/IL-2R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD25/IL-2R alpha at approximately 60 kDa (as indicated). GAPDH (AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Flow, B/N, CyTOF-ready, KO
Applications: WB, Flow, B/N, CyTOF-ready, KO
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF223
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF-223-SP
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DY2438

Mouse CD25/IL-2R alpha DuoSet ELISA, 15 Plate

(10 Publications)
1 Kit (for 15 Plates)
DY2438
N/A CD25/IL-2R alpha [Biotin]
Mu
Species Glossary
Reactivity: Mu
ELISA
Applications: ELISA
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AF2438

CD25/IL-2R alpha Antibody [Unconjugated]

(3 Publications)
100 μg
AF2438
Recombinant Mouse IL‑2 (Catalog # 402-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CD25/IL‑2 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2438). The ND50 is typically 5-20 µg/mL.
Mu
Species Glossary
Reactivity: Mu
WB, IHC, B/N, ELISA(Cap)
Applications: WB, IHC, B/N, ELISA(Cap)
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF2438
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF2438-SP
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MAB1020

CD25/IL-2R alpha Antibody (24212) [Unconjugated]

(5 Publications)
500 μg
MAB1020
In a functional flow cytometry test, Recombinant Human IL-2 Biotinylated protein (Catalog # BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 μg/mL of Mouse Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB1020). Mouse IgG2A Isotype Control (Catalog # MAB003) at 0.5 μg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050).
Hu
Species Glossary
Reactivity: Hu
WB, Flow, B/N, CyTOF-ready
Applications: WB, Flow, B/N, CyTOF-ready
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB1020U-100UG
Alexa Fluor 405 FAB1020V-100UG
Alexa Fluor 488 FAB1020G-100UG
Alexa Fluor 532 FAB1020X
Alexa Fluor 594 FAB1020T-100UG
Alexa Fluor 647 FAB1020R-100UG
Alexa Fluor 700 FAB1020N-100UG
Alexa Fluor 750 FAB1020S-100UG
Allophycocyanin FAB1020A-025
Allophycocyanin FAB1020A-100
Allophycocyanin/Cy7 FAB1020APCCY7
Biotin FAB1020B
DyLight 350 FAB1020D
DyLight 405 FAB1020E
DyLight 488 FAB1020K
DyLight 550 FAB1020L
DyLight 594 FAB1020M
DyLight 650 FAB1020W
DyLight 680 FAB1020Y
DyLight 755 FAB1020Z
FITC FAB1020F
HRP FAB1020H
Janelia Fluor 525 FAB1020JF525
Janelia Fluor 549 FAB1020I
Janelia Fluor 646 FAB1020J
Janelia Fluor 669 FAB1020JF669
PE/Atto594 FAB1020PEATT594
PE/Cy5.5 FAB1020PECY55
PE/Cy7 FAB1020PECY7
PerCP FAB1020C
Phycoerythrin FAB1020P-100
Phycoerythrin FAB1020P-025
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free FAB1020G-100UG
Carrier Free FAB1020P-100
Carrier Free FAB1020A-100
Carrier Free FAB1020A-025
Carrier Free FAB1020P-025
Carrier Free MAB1020-SP
Carrier Free FAB1020R-100UG
Carrier Free FAB1020N-100UG
Carrier Free FAB1020V-100UG
Carrier Free FAB1020U-100UG
Carrier Free FAB1020T-100UG
Carrier Free FAB1020S-100UG
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DY223

Human CD25/IL-2R alpha DuoSet ELISA, 15 Plate

(6 Publications)
1 Kit (for 15 Plates)
DY223
N/A CD25/IL-2R alpha [Biotin]
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
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MAB9926

CD25/IL-2R alpha Antibody (Hu107) [Unconjugated]

100 μg
MAB9926
In a functional flow cytometry test, Recombinant Human IL-2 Biotinylated protein (Catalog # BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 μg/mL of Human Anti-Human CD25/IL-2R alpha (Research Grade Basiliximab Biosimilar) Monoclonal Antibody (Catalog # MAB9926). Human IgG Isotype Control (Catalog # 1-001-A) at 0.5 μg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050).
Hu
Species Glossary
Reactivity: Hu
Flow, B/N, CyTOF-ready
Applications: Flow, B/N, CyTOF-ready
Human Monoclonal
Host: Human Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB9926U-100UG
Alexa Fluor 405 FAB9926V-100UG
Alexa Fluor 488 FAB9926G-100UG
Alexa Fluor 532 FAB9926X
Alexa Fluor 594 FAB9926T-100UG
Alexa Fluor 647 FAB9926R-100UG
Alexa Fluor 700 FAB9926N-100UG
Alexa Fluor 750 FAB9926S-100UG
Allophycocyanin FAB9926A
Allophycocyanin/Cy7 FAB9926APCCY7
Biotin FAB9926B
CoraFluor 1 FAB9926CL1
DyLight 350 FAB9926D
DyLight 405 FAB9926E
DyLight 488 FAB9926K
DyLight 550 FAB9926L
DyLight 594 FAB9926M
DyLight 650 FAB9926W
DyLight 680 FAB9926Y
DyLight 755 FAB9926Z
FITC FAB9926F
HRP FAB9926H
Janelia Fluor 525 FAB9926JF525
Janelia Fluor 549 FAB9926I
Janelia Fluor 646 FAB9926J
Janelia Fluor 669 FAB9926JF669
PE FAB9926P
PE/Atto594 FAB9926PEATT594
PE/Cy5.5 FAB9926PECY55
PE/Cy7 FAB9926PECY7
PerCP FAB9926C
mFluor Violet 450 SE FAB9926MFV450
mFluor Violet 500 SE FAB9926MFV500
mFluor Violet 610 SE FAB9926MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB9926-SP
Carrier Free FAB9926V-100UG
Carrier Free FAB9926U-100UG
Carrier Free FAB9926T-100UG
Carrier Free FAB9926S-100UG
Carrier Free FAB9926R-100UG
Carrier Free FAB9926N-100UG
Carrier Free FAB9926G-100UG
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MAB9927

CD25/IL-2R alpha Antibody (Hu102) [Unconjugated]

(1 Publication)
100 μg
MAB9927
Human PBMCs were treated with PHA (5 µg/mL for 24hr) then stained with (A) Human CD25/IL-2 Ra (Daclizumab Biosimilar) Monoclonal Antibody (Catalog # MAB9927) or (B) Normal Human IgG control antibody (Catalog # 1-001-A), followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antiobdy (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.
Hu
Species Glossary
Reactivity: Hu
Flow, CyTOF-ready
Applications: Flow, CyTOF-ready
Human Monoclonal
Host: Human Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB9927U-100UG
Alexa Fluor 405 FAB9927V-100UG
Alexa Fluor 488 FAB9927G-100UG
Alexa Fluor 532 FAB9927X
Alexa Fluor 594 FAB9927T-100UG
Alexa Fluor 647 FAB9927R-100UG
Alexa Fluor 700 FAB9927N-100UG
Alexa Fluor 750 FAB9927S-100UG
Allophycocyanin FAB9927A
Allophycocyanin/Cy7 FAB9927APCCY7
Biotin FAB9927B
CoraFluor 1 FAB9927CL1
DyLight 350 FAB9927D
DyLight 405 FAB9927E
DyLight 488 FAB9927K
DyLight 550 FAB9927L
DyLight 594 FAB9927M
DyLight 650 FAB9927W
DyLight 680 FAB9927Y
DyLight 755 FAB9927Z
FITC FAB9927F
HRP FAB9927H
Janelia Fluor 525 FAB9927JF525
Janelia Fluor 549 FAB9927I
Janelia Fluor 646 FAB9927J
Janelia Fluor 669 FAB9927JF669
PE FAB9927P
PE/Atto594 FAB9927PEATT594
PE/Cy5.5 FAB9927PECY55
PE/Cy7 FAB9927PECY7
PerCP FAB9927C
mFluor Violet 450 SE FAB9927MFV450
mFluor Violet 500 SE FAB9927MFV500
mFluor Violet 610 SE FAB9927MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB9927-SP
Carrier Free FAB9927V-100UG
Carrier Free FAB9927U-100UG
Carrier Free FAB9927T-100UG
Carrier Free FAB9927S-100UG
Carrier Free FAB9927R-100UG
Carrier Free FAB9927N-100UG
Carrier Free FAB9927G-100UG
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223-2A/CF

Recombinant Human CD25/IL-2R alpha Protein, CF

(3 Publications)
5 μg
Hu
Species Glossary
Reactivity: Hu
Bioactivity
Applications: Bioactivity
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free 223-2A-025/CF
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AB-223-NA

CD25/IL-2R alpha Antibody

(1 Publication)
1 mg
AB-223-NA
Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL‑2 R alpha Polyclonal Antibody (Catalog # AB-223-NA). The ND50 is typically 5-20 µg/mL.
Hu
Species Glossary
Reactivity: Hu
WB, B/N
Applications: WB, B/N
Goat Polyclonal
Host: Goat Polyclonal
Unconjugated  
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