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NB600-308

GFP Antibody

(18 Reviews)

(262 Publications)

0.1 mg

NB600-308

Analysis of GFP in transgenic mouse pancreas in OCT. The blue region in the center is an islet which does not express GFP and is negative. Image from verified customer review.

Analysis of GFP tagged PKA subunits over-expressed in HEK293T cells using GFP antibody. Image from verified customer review.

Staining of GFP+ hepatocytes (brown) transplanted into the liver of a recipient animal using anti-GFP antibody. Cyclin D1 double staining in blue. Image from verified customer review.

Total cell lysates (20ug) with protein of interest tagged with GFP was loaded. Blotted with GFP antibody [HRP] NB600-308H, without any secondary antibody. Image from verified customer review.

Germaria from wt or chico1/KG mutant ovaries endogenously expressing Myc-GFP stained with anti-GFP, anti-1B1, and DAPI. Scale bars, 10 um. (F) Quantification of relative Myc-GFP intensity in germarium region 2B from wt or chico1/KG mutant ovaries. n = 10 germaria for each genotype. Error bars represent SEM. *p 0.05), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 and GAPDH are shown below the graphs d. MM – molecular weight marker, C – tissues obtained from control cows, ADENO – tissues obtained from cows with adenomyosis Image collected and cropped by CiteAb from the following open publication (//www.rbej.com/content/13/1/110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Protein expression and nuclear foci formation in breast cancer cell lines. (a) Cellular protein levels. 53BP1 and Vimentin band intensities were normalized to loading controls. Values for T47D cell extracts, loaded as a reference on each blot, were set to 1. Mean relative Western blot signals per cell line were calculated from two independent experiments. Box plots in the upper panels show mean values (cross), the median (line), and 95% CI (whiskers) for untreated and for 7d Eribulin-treated non-TNBC (N=4) and TNBC cell lines (N=7). The Kruskal–Wallis test did not reveal significant differences in the case of 53BP1. Representative immunoblots are shown in the bottom panels. Vimentin staining was detected in all TNBC but was negative in all non-TNBC cell lines in which BT20 served as a reference. Actin normalized protein levels are indicated above the 53BP1 and Vimentin signals. (b) Focal accumulation of 53BP1 in the nucleus of MBC cell lines. 53BP1 foci were detected by immunofluorescence microscopy in MBC cell lines. The graph presents quantitative data in box plots as in A; ~300–900 nuclei per sample were scored in two independent experiments each, and mean values were calculated for each cell line. *P<0.05, Kruskal–Wallis test, Mann–Whitney U test. Representative immunofluorescence images are displayed in Supplementary Figure S3. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32283863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , , , , ,

Applications: , , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-308AF350
Alexa Fluor 405	NB600-308AF405
Alexa Fluor 488	NB600-308AF488
Alexa Fluor 532	NB600-308AF532
Alexa Fluor 594	NB600-308AF594
Alexa Fluor 647	NB600-308AF647
Alexa Fluor 700	NB600-308AF700
Alexa Fluor 750	NB600-308AF750
Allophycocyanin	NB600-308APC
Allophycocyanin/Cy7	NB600-308APCCY7
Biotin	NB600-308B
DyLight 350	NB600-308UV
DyLight 405	NB600-308V
DyLight 488	NB600-308G
DyLight 550	NB600-308R
DyLight 594	NB600-308DL594
DyLight 650	NB600-308C
DyLight 680	NB600-308FR
DyLight 755	NB600-308IR
FITC	NB600-308F
HRP	NB600-308H
Janelia Fluor 549	NB600-308JF549
Janelia Fluor 646	NB600-308JF646
PE	NB600-308PE
PE/Atto594	NB600-308PEATT594
PE/Cy5.5	NB600-308PECY55
PE/Cy7	NB600-308PECY7
PerCP	NB600-308PCP
mFluor Violet 450 SE	NB600-308MFV450
mFluor Violet 500 SE	NB600-308MFV500
mFluor Violet 610 SE	NB600-308MFV610

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NB100-1770

GFP Antibody

(7 Reviews)

(122 Publications)

0.1 mg

NB100-1770

Analysis using the Biotin conjugate of NB100-1770. Staining of transgenic mouse pancreas, expressing GFP in beta cells. Image courtesy of product review by Dr. Yves Heremans of Vrije Universiteit Brussel.

Analysis of Biotin conjugate of NB100-1770. Tissue: Drosophila melanogaster late stage embryonic central nervous system. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Anti-GFP antibody at a 1:1000 for 1 h at RT.

Analysis using the FITC conjugate of NB100-1770. Lane 1: GFP (50 ug). Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. Block for 30 minutes.

HEK293 cells stable transfected with integrin alpha8-GFP, were immunostained for GFP (green) and counterstained with phalloidin (red) and DAPI (BLUE). ICC/IF image submitted by a verified customer review.

Analysis using the Alkaline Phosphatase conjugate of NB100-1770. Lane 1: GFP (50 ng). Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Predicted/Observed size: 28 kDa for GFP.

9 Poly-GA co-immuno - precipitation with pATM. Aggregated poly-GA selectively co-immunoprecipitates with pATM. Image collected and cropped by CiteAb from the following publication (www.link.springer.com/article/10.1007%2Fs00401-019-02082-0) licensed under a CC-BY license.

E5.5 Hex-GFP transgenic mouse embryo tissue. Primary antibody: Goat anti-GFP at 1:500 dilution. Secondary antibody: Fluorochrome conjugated Anti-goat IgG antibody at 1:10,000 for 45 min at RT. Staining: GFP as green fluorescent signal with DAPI blue counterstain.

IF analysis of GFP in mouse brain. Image courtesy of product review by Tatyana Pivneva.

Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS o/n, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Primary antibody: Goat anti-GFP was used at 1:500 dilution in free floating immunohistochemistry to detect GFP. Secondary antibody: Fluorochrome conjugated Anti-goat IgG antibody at 1:500 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.

Analysis of DyLight 488 conjugate of NB100-1770. Human breast carcinoma tissue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Anti-Histone and Anti-Tubulin antibody at 10 ug/mL for 1 h at RT. Secondary antibody: DyLight 488.

GFP-positive transplanted NPCs have morphological features of hippocampal pyramidal neurons at day 90 after grafting. IHC image submitted by a verified customer review.

Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. Primary antibody: GFP antibody at 1 ug/ml for 1 h at room temperature. Secondary antibody: IRDye(R) 800 conjugated Donkey-a-Goat IgG [H&L] MX7 () secondary antibody at 1:2,500 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa, 33 kDa for GFP. Other band(s): none.

Western Blot of GFP Antibody. Lane 1: Opal Prestained Molecular Weight Marker

Multi-lysate Western Blot of GFP Antibody. Marker: Opal Pre-stained ladder

hnRNPA3 knockout enhances DNA damage in DPR expressing HeLa cells. a Immunocytochemical detection of gammaH2AX foci in GFP-tagged DPR-expressing HeLa WT and A3KO HeLa cells. b Fold change of gammaH2AX foci in HeLa WT and A3KO cells expressing the indicated DPRs relative to HeLa WT cells with GFP expression. N = 47-127 cells from 2 biological replicates. c Immunocytochemical detection of pATM foci in GFP-tagged DPR-expressing HeLa WT and A3KO HeLa cells. d Fold change of pATM foci in HeLa WT and A3KO cells expressing the indicated DPRs relative to HeLa WT cells with GFP expression. N = 71-263 cells from 2 biological replicates. GFP EGFP transfected, GA EGFP-tagged poly-GA 175 repeats transfected, GR EGFP-tagged poly-GR 177 repeats transfected, PR: EGFP-tagged poly-PR 176 repeats transfected. All graphs are shown as mean +/- SEM. *p < 0.05, **p < 0.01; one-way ANOVA and Tukey's post-hoc test. Scale bar 10 um Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31642962), licensed under a CC-BY licence.

HIF1 alpha and PFKFB3 are protective against hIAPP mediated beta -cell death. a Representative immunoblotting (left) and quantification (right) of the whole cell extract (WCE) from asynchronous INS 832/13 cells treated with either HIF1 alpha inhibitor KC7F2 (10 μM) or PFKFB3 siRNA (75 nM) for 56 h versus control. Cells were transduced with hIAPP adenovirus (75 MOI) or LacZ adenovirus as control (75 MOI) for last 32 h of culture. DMSO or CTRL siRNA, respectively were added to LacZ or hIAPP to equal DMSO or PFKFB3 siRNA used in experimental groups. GAPDH was used as a loading control. Data are presented as mean ± SEM, n = 4, *p < 0.05, **p < 0.01. b Quantification of DNA content distribution after FACS analysis of INS 832/13 cells treated as described in (a). Data are the mean ± SEM, n = 4, *p < 0.05, **p < 0.01. c The frequency of TUNEL positive INS 832/13 cells treated as described in (a). Data are the mean ± SEM, n = 4 in (a) and (b), and n = 3 independent experiments in (c). Statistical significance was analyzed by one-way ANOVA test with Tukey’s post-test (*p < 0.05, **p < 0.01). See Supplementary Fig. 10 for additional supportive data Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31213603), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200× magnification) of (A) S100a9, (B) beta -catenin, (C) c-Myc, (D) p-Akt, (E) p-Smad2, and (F) Cxcl5 in normal control, IgG Ab, and anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 µm. Staining scores were determined by semi-quantitative optical analysis. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , , , , , ,

Applications: , , , , , , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alkaline Phosphatase	NB600-1502
Biotin	NB100-1678
DyLight 488	NBP1-69969
FITC	NB100-1771
FITC	NB100-1771SS
HRP	NB600-313
Rhodamine	NBP1-97327-500ug
Texas Red	NB120-6660

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NBP1-96752

mCherry Antibody (1C51)

(3 Reviews)

(79 Publications)

0.1 ml

NBP1-96752

Immunohistochemistry: mCherry Antibody (1C51) [NBP1-96752] - Cadherin-2 is required cell autonomously for caudal migration of FBMNs. (A-I) Whole-mount immunocytochemistry showing dorsal views of Tg (isl1:GFP) (A-C) and Tg (isl1:cdh2_EC-mCherry)vc25 transgenic embryos (D-I) at 38 hpf embryos. Embryos are labeled with a-GFP (green) (A,D,G) and a-mCherry (red) (B,E,H) antibodies. (A-C) Wild-type Tg (isl1:GFP) embryos with FBMNs fully migrated into r6. (D-I) Defective caudal migration of FBMNs in Tg (isl1:GFP)/Tg(isl1:cdh2_EC-mCherry) vc25 embryos carrying one copy of the transgene (hemizygous) or two copies (homozygous). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0164433) licensed under a CC-BY license.

Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Analysis of HEK293 cell lysates and recombinant protein solutions using mCherry antibody, dilution 1:1,000 (Green). [1] protein standard, [2] HEK293, [3] HEK293 cells transfected with mCherry-HA construct, [4] mCherry recombinant protein, [5] GFP recombinant protein, and [6] HEK293 transfected with GFP construct. Major band at about 30 kDa corresponds to mCherry protein (predicted molecular weight: 27 kDa). mCherry antibody does not react with GFP protein. The same blot was simultaneously probed with chicken HSP60 pAb, dilution 1:5,000 in red which reveals band at 60 kDa seen only in cell lysates.

Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Fluorescent signals and immunoblots of the dual fluorescence reporter of cup-5 32-UTR in WT worms and mir-83(-) mutants at day 1 of adulthood. Quantification is from the western blots. GFP blots and WT worms serve as controls for loading and normalization respectively. Scale bar: 100 i1/4m. n = 3 independent experiments. Image collected and cropped by Citeab from the following publication (A secreted microRNA disrupts autophagy in distinct tissues of Caenorhabditis elegans upon ageing. Nat Commun (2019) licensed under a CC-BY license.

$Western Blot: mCherry Antibody (1C51) [NBP1-96752] - Threonine 276 is required for the intramolecular interaction, and for inhibition of membrane binding. Mutation of threonine 276 to aspartic acid promotes the association of cytohesin 2 with membranes. MDCK cells were transfected with constructs encoding mCherry-tagged wild-type or T276D cytohesin 2 and fractionated into cytosol and total membranes. The fractions were Western blotted with mouse anti-mCherry, mouse anti-E-cadherin and mouse anti-actin. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pone.0082084) licensed under a CC-BY license.$

Immunocytochemistry/Immunofluorescence: mCherry Antibody (1C51) [NBP1-96752] - HEK293 cells transfected with mCherry and visualized in red. The cells were stained with NBP1-96752 in the green channel, and visualized using a confocal microscope. Transfected cells are yellow, showing overlap of the mCherry and NBP1-96752. Untransfected HEK293 cells do not express Cherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue).

Western Blot: mCherry Antibody (1C51) [NBP1-96752] - WB assay of the crude extract of HEK293 cells transfected with pFin-EF1-mCherry vector (lane +) and an equal amount of protein extract from untransfected HEK293 cells (lane -). NBP1-96752 binds a major band running at about 28 kDa (observed molecular weight) corresponding to intact full-length mCherry. The two other bands are clearly processed forms of mCherry as they are not present in non-transfected HEK293 cells.

Immunohistochemistry-Frozen: mCherry Antibody (1C51) [NBP1-96752] - Mouse Bone Marrow Sections (Femur). Fixed-frozen and decalcified. tdTomato reporter transgenic mice. tdTomato in hematopoietic cells were detected by anti-mCherry antibody. Antibody is cross-reactive and works well for fixed-frozen bone marrow. Background is low. IHC-Fr image submitted by a verified customer review.

The oligomerization state of overexpressed Cav1 varies as a function of its tag. COS-7 cells expressing the indicated constructs were lysed in digitonin and subjected to BN-PAGE followed by western blotting for Cav1 (red) and either GFP, mCherry or myc (green). A) Cells were either left untransfected ('control') or transfected with EGFP, Cav1-GFP or P132L-GFP. B) As in (A) except cells were transfected with the indicated mCherry constructs. C) As in (A) except cells were transfected with Cav1-myc or P132L-myc. Figures are representative of two independent experiments. Red arrows indicate the high molecular weight band positive for both tag antibodies and Cav1 antibodies (h1-97 or 2297). Black arrows indicate the high molecular weight band only positive for Cav1 antibodies (h1-97 or 2297). Green arrows indicate the low molecular weight bands only positive for FP tag antibodies. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25639341), licensed under a CC-BY licence.

Mis18alpha:Mis18beta-hexamer mediates dimerization of M18BP1. (D) Western blot results of co-immunoprecipitation experiments using GFP-Trap_A beads. HeLa CENP-A-SNAP + EGFP-M18BP11-140-P2A-T2A-mCherry-M18BP11-140, EGFP-M18BP11-140/T40D/S110E-P2A-T2A-mCherry-M18BP11-140/T40D/S110E, GST-EGFP-M18BP11-140-P2A-T2A-GST-mCherry-M18BP11-140, or GST-EGFP-M18BP11-140/T40D/S110E-P2A-T2A-GST-mCherry-M18BP11-140/T40D/S110E were analyzed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28059702), licensed under a CC-BY licence.

Fluorescent signals and immunoblots of the dual fluorescence reporter of cup-5 3'-UTR in WT worms and mir-83(-) mutants at day 1 of adulthood. Quantification is from the western blots. GFP blots and WT worms serve as controls for loading and normalization respectively. Scale bar: 100 um. n = 3 independent experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31645592), licensed under a CC-BY licence.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-96752AF350
Alexa Fluor 405	NBP1-96752AF405
Alexa Fluor 488	NBP1-96752AF488
Alexa Fluor 532	NBP1-96752AF532
Alexa Fluor 594	NBP1-96752AF594
Alexa Fluor 647	NBP1-96752AF647
Alexa Fluor 700	NBP1-96752AF700
Alexa Fluor 750	NBP1-96752AF750
Biotin	NBP1-96752B
DyLight 350	NBP1-96752UV
DyLight 405	NBP1-96752V
DyLight 488	NBP1-96752G
DyLight 550	NBP1-96752R
DyLight 594	NBP1-96752DL594
DyLight 650	NBP1-96752C
DyLight 680	NBP1-96752FR
DyLight 755	NBP1-96752IR
FITC	NBP1-96752F
HRP	NBP1-96752H
Janelia Fluor 549	NBP1-96752JF549
Janelia Fluor 646	NBP1-96752JF646
mFluor Violet 450 SE	NBP1-96752MFV450
mFluor Violet 500 SE	NBP1-96752MFV500
mFluor Violet 610 SE	NBP1-96752MFV610

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NBP2-25157

mCherry Antibody

(4 Reviews)

(71 Publications)

0.1 ml

NBP2-25157

Immunohistochemistry: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in other regions of the CNS. Optic tracts at P0 visualized by mCherry staining (white arrows). (BAE ) Co-localization experiments with mCherry (red) and pan-Brn3 (green) antibodies at P0. (CAE ) Co-localization with mCherry (red) and Tuj1 (green) in the trigeminal ganglia at E13. 5. Arrows indicate mCherry+ Tuj+ neurons. Image collected and cropped by Citeab from the following publication (A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression. Int J Mol Sci (2019)) licensed under a CC-BY license.

Immunohistochemistry: mCherry Antibody [NBP2-25157] - Activation of TRPV1 receptors in ARC POMC neurons reduces food intake. Expression of Gi/o-DREADD in POMC neurons in the ARC (top left panel, scale bar: 20 um). Arrowheads represent POMC neurons that co-expressed mCherry (n = 493 out of 529 mCherry-positive neurons, n = 3 mice). Treatment with CNO (10 uM) hyperpolarized POMC neurons (top right panel; Control, -40.7 +/- 1.5 mV; CNO, -47.1 +/- 2.3 mV, n = 6 neurons, **p < 0.01). Scale bar: 25 mV, 2 min. Bottom panel: In the presence of CNO, treatment of the TRPV1 agonist was not able to depolarize POMC neurons (n = 3 neurons). Scale bar: 20 mV, 100 s. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pbio.2004399) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in the adult retina. Flat-mounted retina labeled with anti-mCherry antibody. (BAE ) mCherry (red) and Brn3 (teal) colocalization. Yellow arrow indicates a Brn3+ mCherry-cell. (CAE ) mCherry (red) and RBPMS (teal) colocalization. Yellow arrows indicate RBPMS+ mCherry-cell bodies. (DAE ) mCherry (red) and Tuj1 (teal) colocalization. Image collected and cropped by Citeab from the following publication (A Novel Reporter Mouse Uncovers Endogenous Brn3b Expression. Int J Mol Sci (2019)) licensed under a CC-BY license.

Western Blot: Rabbit Polyclonal mCherry Antibody [NBP2-25157] - Analysis of HEK293 cell lysates using rabbit polyclonal mCherry antibody, dilution 1:3000, in green, and mouse mAb to GAPDH, dilution 1:2000, in red: [1] protein standard, [2] HEK293 control cells, [3] HEK293 cells transfected with pCI-Neo-mod vector expressing two tdTomato protein domains, [4] HEK293 cells transfected with pCI-mod vector expressing one mCherry-HA protein domain, and [5] HEK293 cells transfected with pCI-Neo-mod vector expressing one GFP domain. The rabbit polyclonal mCherry antibody recognizes tdTomato and mCherry proteins revealing major bands at about 60kDa and 30kDa, in green, respectively, but does not recognize GFP. The red band at 37kDa corresponds to GAPDH protein here used as a loading control.

Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25157] - Brn3b-mCherry expression in other regions of the CNS. (A) Optic tracts at P0 visualized by mCherry staining (white arrows). (B,BAE ) Co-localization experiments with mCherry (red) and pan-Brn3 (green) antibodies at P0. (C,CAE ) Co-localization with mCherry (red) and Tuj1 (green) in the trigeminal ganglia at E13. 5. Arrows indicate mCherry+ Tuj+ neurons. Low magnification image of a horizontal E13. 5 whole-head section. EAE

Western Blot: mCherry Antibody [NBP2-25157] - PKN3 overexpression regulates growth of MEFs, and this effect requires PKN3AE p130Cas interaction. Immunoblotted lysates from MEFs p130CasA/A or MEFs p130CasA/A reAE expressing p130Cas (p130Cas+) treated by Doxycycline (Dox) to induce expression of mCherryAE PKN3 or mCherry alone. p130Cas presence was detected by aniAE p130Cas antibody and mCherry epitope by anti mCherry antibody. Dynamics of mCherry PKN3 expression after supplementation with Dox shown by immunoblot with anti mCherry antibody. Image collected and cropped by Citeab from the following publication (The interaction of p130Cas with PKN3 promotes malignant growth. Mol Oncol (2019)) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: mCherry Antibody [NBP2-25157] - Staining of a mouse melanoma with over expression of mCherry in lung tissue. IHC-P image submitted by a verified customer review.

Western Blot: mCherry Antibody [NBP2-25157] - 3 cells transfected with pFin-EF1-mCherry vector, in the lane marked '+'. HEK293 cells which were not transfected with this vector show no protein band in lane marked '-'.

Western Blot: mCherry Antibody [NBP2-25157] - Detection of mCherry-fused protein: MDA-MB-231 cell lysates with and without expression of mCherry-fused protein. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25157] - HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with NBP2-25157 is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome. Blot and transfected cells courtesy of the Semple-Rowland lab at the University of Florida.

Fluorescence Imaging: mCherry Antibody [NBP2-25157] - Analysis of a Drosophila wing imaginal disc. Image submitted by a verified customer review.

Expression and localization of TRPA1 channels in wild-type (WT) and APP/PS1 Tg mice. (a) Brains were harvested from WT and APP/PS1 Tg mice at 8 months old. Western blot analysis of protein levels of TRPA1 and alpha -tubulin. Data are mean ± SEM from 6 mice in each group. *, P < 0.05 vs. WT mice. (b, c) Immunohistochemistry of specimens of cortex and hippocampus from 8-month-old WT and APP/PS1 Tg mice with the antibodies anti-TRPA1, anti-vWF (endothelial cell marker), anti-NeuN (neuron marker), anti-GFAP (astrocyte marker) and anti-IBA-1 (microglia marker), then FITC- or Texas red-conjugated secondary antibody. Bar = 50 μm. vWF-positive cells denoted endothelial cells, NeuN-positive cells denoted neurons, and GFAP-positive cells denoted astrocytes, as indicated by arrowheads, stars or arrows, respectively Image collected and cropped by CiteAb from the following open publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016-0557-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Targeted disruption of PTEN in vitro and in vivo induces activation of pS6 and BMAL1A. and B. Targeted inhibition of PTEN using siRNA results in concentration-dependent inhibition of PTEN protein in HNSCC cells. HNSCC cells show accumulation of BMAL1 and pS6 in response to PTEN inhibition. C. Immunofluorescence assay to detect BMAL1 in PTEN conditional knockout mice (K14cre PtenF/F) and control littermates. Immunofluorescences and graphic show high accumulation of nuclear BMAL1 in K14cre PtenF/F mice compared to control mice (K14cre). Scale bars represent 50 μm. Black and white images depict cells positive for BMAL1 in K14cre PtenF/F mice (arrows) compared to few BMAL-positive cells in control mice (arrowhead). Scale bars represent 10 μm. (**p<0.01). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/27285754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25157AF350
Alexa Fluor 405	NBP2-25157AF405
Alexa Fluor 488	NBP2-25157AF488
Alexa Fluor 532	NBP2-25157AF532
Alexa Fluor 594	NBP2-25157AF594
Alexa Fluor 647	NBP2-25157AF647
Alexa Fluor 700	NBP2-25157AF700
Alexa Fluor 750	NBP2-25157AF750
Allophycocyanin	NBP2-25157APC
Biotin	NBP2-25157B
DyLight 350	NBP2-25157UV
DyLight 405	NBP2-25157V
DyLight 488	NBP2-25157G
DyLight 550	NBP2-25157R
DyLight 594	NBP2-25157DL594
DyLight 650	NBP2-25157C
DyLight 680	NBP2-25157FR
DyLight 755	NBP2-25157IR
FITC	NBP2-25157F
HRP	NBP2-25157H
Janelia Fluor 549	NBP2-25157JF549
Janelia Fluor 646	NBP2-25157JF646
PE	NBP2-25157PE
PerCP	NBP2-25157PCP
mFluor Violet 450 SE	NBP2-25157MFV450
mFluor Violet 500 SE	NBP2-25157MFV500
mFluor Violet 610 SE	NBP2-25157MFV610

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NB100-1614

GFP Antibody

(6 Reviews)

(73 Publications)

0.1 ml

NB100-1614

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - Staining of cultured hippocampal neuron from rat. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - HeLa cells transiently expressing GFP were visualized using GFP antibody (green). Nuclei were counterstained with DAPI. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - IF analysis of GFP in mouse trigeminal ganglia, cornea. Image courtesy of product review by Jessica Newsom.

Immunohistochemistry: GFP Antibody [NB100-1614] - 5dpf Tg(brn3c:GFP) larvae were incubated with vehicle alone (DMSO, A), 400uM of cisplatin (CP, B), pre-treated with Qx for 2 hours and then incubated with CP for 6 hours (Qx, CP, C,E,G) or pre-treated with Qx for 2 hours and then co-treated with Qx and CP for 6 more hours (Qx+CP, D,F,H). Animals were fixed and immunostained for GFP (green) and otoferlin (red). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-33520-w), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - IF analysis of GFP in rat cortical culture (2 weeks old). ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - LNCaP human prostate cancer cell line imaged after transfection with GFP expressing plasmid. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1614] - Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (RED).

Immunohistochemistry-Paraffin: GFP Antibody [NB100-1614] - Pyramidal neurons in the hippocampal formation of a neonatal mouse brain. Tissue was paraformaldehyde-fixed (4%) and paraffin-embedded. GFP staining is in green in left panel.

Immunohistochemistry-Paraffin: GFP Antibody [NB100-1614] - Embyonic mouse brain section. GFP in green, DAPI in grey. IHC-P image submitted by a verified customer review.

Immunohistochemistry: GFP Antibody [NB100-1614] - (J) control. (K) Neo 200uM 30min. (L) GM 50uM 1 hour. (M) CP: 400uM 2 hours. (N) Qx 300uM 8 hours. (O) Qx 300uM 8 hours + Neo 200uM 30min. (P) Qx 300uM 8 hours + GM 50uM 1 hour. (Q) Qx 300uM 8 hours + CP 400uM 2 hours. Asterisks denote neuromast supporting cells positive for BrdU. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-33520-w), licensed under a CC-BY license.

Comparison between GFP-immunoreactivity using NB100-1614 (left panel in red) and autofluorescence (right panel in green). In this case, the cortical neuron in this unfixed thick section was first photographed for GFP autofluorescence (left), and then the section was fixed (4% paraformaldehyde) and immunostained for GFP-immunoreactivity (1:1000 dilution) using Texas Red-goat anti-chicken IgY antibodies as a secondary. The same cell (left) was then identified. Far right: Western blot showing specific immunolabeling of the GFP protein.

Immunohistochemistry: Chicken Polyclonal GFP Antibody [NB100-1614] - Chicken Polyclonal GFP Antibody on mouse cancer tissue. DAPI (Red) and Cytopastmic GFP stain (Green). Primary antibody dilution: 1:200 in a 200 um slice. Image from a verified customer review.

Intact MOR function in the Oprm1-Cre line. c) Confocal imaging of RNAscope probes targeting eGFP (green) and Oprm1 (purple) mRNAs in addition of DAPI staining (blue) shows colocalization of the two transcripts in dorsal striatum sections of Oprm1Cre/Cre mice (inset). Dashed lines delimit examples of MOR-EGFP/Cre-positive neurons. Magnification, 60x with immersion oil. Scale bar, 5 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32381649), licensed under a CC-BY licence.

Reptin depletion reduces the recruitment on chromatin of BRCA1 and 53BP1 after gamma ray irradiation.HuH7 cells stably expressing a doxycycline-inducible Reptin shRNA were treated with doxycycline (sh Reptin +) or left untreated (sh Reptin-) for 4 days. (A) Representative images of 53BP1 and BRCA1 foci in HuH7 cells detected using immunofluorescence 2h after gamma ray irradiation. (B) The bars represent the mean number of foci per cell from two independent experiments (>200 cells were counted per experiment). Expression levels of BRCA1 and 53BP1 were assessed by Western Blot on whole cell extracts 4 days after doxycycline treatment. A representative picture is shown in (C). The migration positions of molecular weight standards (in kDa) are indicated on the left. (D) Quantification of 3 Western blot experiments. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0123333), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Frozen: Chicken Polyclonal GFP Antibody [NB100-1614] - Image depicting GFP in magenta and CD31 (Catalog # AF3628) in red. Tissue is a fixed frozen section of sciatic nerve obtained from a S100A4 GFP mouse. NB100-1614 was diluted 1 in 10000 and was left on tissue sections overnight at 4 degrees Celsius. Secondary antibody was donkey anti-chicken conjugated to Alexa 647. Image from a verified customer review.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB600-597

GFP Antibody (9F9.F9)

(3 Reviews)

(54 Publications)

0.1 mg

NB600-597

Immunocytochemistry/Immunofluorescence: GFP Antibody (9F9.F9) [NB600-597] - GFP-transducted 293T cells. Image from verified customer review.

Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Lane 1: Molecular Weight Markers. Lane 2: GFP. Load: 10ng per lane. Primary antibody: Ms Anti-GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: Mouse HRP secondary antibody at 1:40,000 for 30 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa, 27 kDa for epitope tag GFP. Other band(s): None.

Western Blot: GFP Antibody (9F9.F9) [NB600-597] - All lanes have picogram amount recombinant GFP protein spiked into HeLa cell-derived lysates Lane 1: 64pg 2pg Lane 3: 16pg Lane 4: 8pg Lane 5: 4pg Lane 6: 2pg Lane 7: 1g Lane 8: 0pg. Primary antibody: anti-GFP monoclonal antibody at 1:400 for overnight at 4C. Secondary antibody: HRP-conjugated anti-Mouse IgG was performed at a dilution of 1:20,000 for 1h at 4C. Block: TTBS supplemented with 1% BSA for 1 h at 4C. Predicted/Observed size: 27 kDa for GFP. Other b&(s): none.

Immunohistochemistry: GFP Antibody (9F9.F9) [NB600-597] - Used 1:5000 As referenced in: Lu S-M, Tremblay M-E` , King IL, Qi J, Reynolds HM, et al. (2011) HIV-1 Tat-Induced Microgliosis and Synaptic Damage via Interactions between Peripheral and Central Myeloid Cells. PLoS ONE 6(9): e23915. doi:10.1371/journal.pone.0023915 Image using the Biotin format of this antibody.

Western Blot: GFP Antibody (9F9.F9) [NB600-597] - Expression of GFP in CHO-K1 cells trasfected with GFP. Image from verified customer review.

Immunoprecipitation/Western Blot using GFP Protein. Lane 1: Opal Prestained Molecular Weight Marker

Western blot of GFP Antibody (9F9.F9). Lane 1: Opal Prestained Molecular Weight Marker

Western blot of Mouse Anti-GFP Antibody. Lane 1: Thermo SuperSignal Molecular Weight Marker. Lane 2: GFP protein

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , , , , ,

Applications: , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	NB110-40670

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NB600-303

GFP Antibody

(3 Reviews)

(54 Publications)

0.05 ml

NB600-303

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-303] - Analysis of FITC conjugate of NB600-303. Staining of mouse spleen cells Tissue: spleen cells infected with MHV68-H2bYFP. As seen in: Collins CM, Speck SH (2012) Tracking Murine Gammaherpesvirus 68 Infection of Germinal Center B Cells.

Western Blot: GFP Antibody [NB600-303] - Analysis using the FITC conjugate of NB600-303. Detection of Lane 1: GFP. Load: 50 ug per lane. Primary antibody: none. Secondary antibody: Fluorescein conjugated Anti-GFP at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa.

Western Blot: GFP Antibody [NB600-303] - WB analysis of GFP in various lysates. Image courtesy of product review by Emilie Leclerc.

Immunoprecipitation: GFP Antibody [NB600-303] - IP analysis of GFP in HeLa cell lysate. Image courtesy of anonymous customer review.

Western Blot: GFP Antibody [NB600-303] - Analysis of GFP HRP expression in HeLa/GFP cell lysate.

Western Blot: GFP Antibody [NB600-303] - Analysis using Biotin conjugate of NB600-303. Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti- Biotin Conjugated secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blotting for 45 min at RT. HRP Streptavidin was used at 1:40,000 in incubated with blocking buffer for 30 min at 20C. Block: 5% Blotto 30 min at 20C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.

Immunohistochemistry: GFP Antibody [NB600-303] - Eight-week-old Lgr5-GFP mice were injected with CCL4 and rHGF or/and rRspo1 proteins (0.1 ug/mice/time), as indicated in a, Lgr5 expression was measured using an immunofluorescent assay with anti-GFP antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-01341-6) licensed under a CC-BY license.

Western Blot: GFP Antibody [NB600-303] - Lane 1: 293FT cells transfected with CDK4 dominant negative. Lane 2: 293FT cells poitive control. Load: 25 ug per lane. Primary antibody: GFP antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa for GFP.

Immunocytochemistry/Immunofluorescence: GFP Antibody [NB600-303]

Western Blot of GFP Antibody. Lane 1: 293FT cells transfected with CDK4 dominant negative. Lane 2: 293FT cells poitive control. Load: 25 ug per lane. Primary antibody: GFP antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa for GFP.

Western Blot of Rabbit anti-GFP antibody. Lane 1: Wild type GFP (0.1 ug) was used to spike HeLa whole cell lysate. Lane 2: none. Load: 30 ug per lane. Primary antibody: GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) Goat-a-Rabbit IgG [H&L] MX10 () at 1:10,000 for 45 min at RT. Block: 5% BLOTTO in PBS overnight at 4C. Predicted/Observed size: 27 kDa for epitope tag GFP. Other band(s): none.

The polo-boxes of Plk4 are not sufficient to mediate STIL binding.(A) Scheme of Flag-Plk4 fragments. (B) After overexpression of Flag-Plk4-fragments (A) and GFP-STIL in HEK293T cells, cell lysates were subjected to immunoprecipitations using anti-Flag antibodies. Coprecipitation of GFP-STIL with Flag-Plk4 fragments was detected by western blotting using anti-GFP and anti-Flag antibodies.

Lgr5+ liver stem cells transplantation decreased liver fibrosis. b) Lgr5+ cells were isolated from Lgr5-GFP mice treated with CCL4 by FACS assay for transplantation (left). The liver Lgr5 expression was stained using anti-GFP antibody in mice with Lgr5+ cells or PH transplantation (right) on day 40. n = 10 mice, scale bars, 200 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29079780), licensed under a CC-BY licence.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	NB100-1365
FITC	NB100-1183-0.025mg
FITC	NB100-1183

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NBP2-25158

mCherry Antibody

(1 Review)

(55 Publications)

0.1 ml

NBP2-25158

Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25158] - HEK293 cells transfected with mCherry, stained with mCherry antibody and viewed in a confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red, and staining with the mCherry antibody is shown in Green. The green antibody staining is only seen cells which express mCherry, and the superimposition of the green and red results in an orange signal. Interestingly stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules releasing the low molecular weight mCherry fluorochrome.

Western Blot: mCherry Antibody [NBP2-25158] - Analysis of NBP2-25158. Lane 1. Lysate of HEK293 cells transfected with pFin-EF1-mCherry vector. There is a strong clean band at about 29kDa corresponding to mCherry (predicted molecular weight ~27kDa). Lane 2, lysate of untransfected HEK293 cells show no protein bands. The NBP2-25158 antibody was used at 1:2,000 dilution.

Western Blot: mCherry Antibody [NBP2-25158] - Analysis of HEK293 cell lysates, and recombinant protein solutions using chicken mCherry pAb, dilution 1:2000 (Green). [1] protein standard, [2] HEK293, [3] HEK293 cells transfected with an mCherry-HA constract, [4] pure recombinant mCherry protein (predicted molecular weight ~ 27kDa), [5] pure recombinant GFP protein and [6] HEK293 transfected with a GFP construct. The major band at about 30kDa corresponds to mCherry-HA protein and the slightly larger recombinant form runs at about 33kDa due to presence of a His tag and other vector derived sequence. The mCherry antibody reacts strongly with both mCherry constructs and does not react with GFP protein. The blot was simultaneously probed with mouse HSP60 mAb, dilution 1:10000 (Red), which reveals a band at 60kDa only in the cell lysates.

<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: mCherry Antibody [NBP2-25158] - Dox-inducible DN Lats2-IRES-mCherry transgenic embryos were imaged before Dox treatment (top panel) and the same embryo was imaged following 24 hr of Dox live (middle panel) and fixed/stained for lineage markers (bottom panel). A representative embryo is shown for each stage. Live mCherry is shown as an extended focus image, immunofluorescence stainings shown as single plane images. mCherry positive ICMs in mosaic transgenic embryos are circled with a dotted line. Arrow points to a rare ICM cell in a 64 cell stage-induced embryo with weak Cdx2 expression, which also co-expressed an ICM marker. Scale bar: 25 um. n indicates number of transgenic embryos analyzed. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28226240/) licensed under a CC-BY license.

Western blot analysis of HEK293 cell lysates using chicken pAb to mCherry, NBP2-25158, dilution 1:2,000, in green, and rabbit pAb to GAPDH, RPCA-GAPDH, dilution 1:5,000, in red: [1] protein molecular weight standard, [2] untransfected HEK293 control cells, [3] HEK293 cells transfected with pCI-Neo-mod vector expressing two tdTomato protein domains, [4] HEK293 cells transfected with pCI-Neo-mod vector expressing one mCherry-HA protein domain, and [5] HEK293 cells transfected with pCI-Neo-mod vector expressing one GFP domain. NBP2-25158 recognizes tdTomato and mCherry proteins revealing major bands at about 60kDa and 30kDa, but does not recognize GFP. The red band at 37kDa corresponds to GAPDH protein here used as a loading control.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , ,

Applications: , , , ,

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB600-335

c-Myc Antibody - BSA Free

(1 Review)

(55 Publications)

0.1 ml

NB600-335

Western Blot: c-Myc Antibody [NB600-335] - Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-c-myc antibody NB600-335 at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 20 ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the Alexa Fluor 488 format of this antibody.

Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.4 ug/mL (1:25,000).

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Agarose	NB600-342
Alexa Fluor 350	NB600-335AF350
Alexa Fluor 405	NB600-335AF405
Alexa Fluor 488	NB600-335AF488
Alexa Fluor 532	NB600-335AF532
Alexa Fluor 594	NB600-335AF594
Alexa Fluor 647	NB600-335AF647
Alexa Fluor 700	NB600-335AF700
Alexa Fluor 750	NB600-335AF750
Allophycocyanin	NB600-335APC
Biotin	NB600-335B
DyLight 350	NB600-335UV
DyLight 405	NB600-335V
DyLight 488	NB600-335G
DyLight 550	NB600-335R
DyLight 594	NB600-335DL594
DyLight 650	NB600-335C
DyLight 680	NB600-335FR
DyLight 755	NB600-335IR
FITC	NB600-335F
HRP	NB600-341
HRP	NB600-335H
Janelia Fluor 549	NB600-335JF549
Janelia Fluor 646	NB600-335JF646
PE	NB600-335PE
PerCP	NB600-335PCP

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NBP2-22111

GFP Antibody (4B10B2) - BSA Free

(2 Publications)

0.1 ml

NBP2-22111

Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Expression of GFP in HEK293T cells. This image was submitted via customer Review. Image using the HRP form of this antibody.

Immunocytochemistry/Immunofluorescence: GFP Antibody (4B10B2) [NBP2-22111] - Analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP mouse mAb .

Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Analysis using GFP mouse mAb against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).

Flow Cytometry: GFP Antibody (4B10B2) [NBP2-22111] - Analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP mouse mAb .

Western Blot: GFP Antibody (4B10B2) [NBP2-22111] - Analysis using GFP mouse mAb against extracts from HCC827 cells, untransfected (1) and transfected with GFP (2).

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22111AF350
Alexa Fluor 405	NBP2-22111AF405
Alexa Fluor 488	NBP2-22111AF488
Alexa Fluor 532	NBP2-22111AF532
Alexa Fluor 594	NBP2-22111AF594
Alexa Fluor 647	NBP2-22111AF647
Alexa Fluor 700	NBP2-22111AF700
Alexa Fluor 750	NBP2-22111AF750
Allophycocyanin	NBP2-22111APC
Allophycocyanin/Cy7	NBP2-22111APCCY7
Biotin	NBP2-22111B
DyLight 350	NBP2-22111UV
DyLight 405	NBP2-22111V
DyLight 488	NBP2-22111G
DyLight 550	NBP2-22111R
DyLight 594	NBP2-22111DL594
DyLight 650	NBP2-22111C
DyLight 680	NBP2-22111FR
DyLight 755	NBP2-22111IR
FITC	NBP2-22111F
HRP	NBP2-22111H
Janelia Fluor 549	NBP2-22111JF549
Janelia Fluor 646	NBP2-22111JF646
PE	NBP2-22111PE
PE/Atto594	NBP2-22111PEATT594
PE/Cy5.5	NBP2-22111PECY55
PE/Cy7	NBP2-22111PECY7
PerCP	NBP2-22111PCP
mFluor Violet 450 SE	NBP2-22111MFV450
mFluor Violet 500 SE	NBP2-22111MFV500
mFluor Violet 610 SE	NBP2-22111MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22111SS
Azide and BSA Free	NBP2-80750

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NBP2-43575

GFP Antibody (859)

(29 Publications)

0.1 ml

NBP2-43575

Western Blot: GFP Antibody (859) [NBP2-43575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (5 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with GFP antibody (859) diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: GFP Antibody (859) [NBP2-43575] - GFP-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: GFP stained by GFP antibody [859] diluted at 1:2000.Blue: Hoechst 33342 staining.

Western Blot: GFP Antibody (859) [NBP2-43575] - Non-transfected (-) and transfected (+) 293T whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GFP antibody [859] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: GFP Antibody (859) [NBP2-43575] - Analysis of GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GFP is expressed in the tranfected cell Red: .GFP-recombinant protein stained by GFP-tag antibody diluted at 1:2000. Blue: Hoechst 33342 staining.

ELISA: GFP Antibody (859) [NBP2-43575] - Coating Ab: mouse anti-GFP (2ug/well) Sample: GFP protein Detected Ab: rabbit anti-GFP Conjugated Ab: goat anti-rabbit IgG-HRP (1:20000)

Immunoprecipitation: GFP Antibody (859) [NBP2-43575] - Analysis of 400 ug cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 ug of GFP tag antibody 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-49888

Tat-Beclin 1 D11 Autophagy Inducing Peptide - Retroinverso form

(1 Review)

(18 Publications)

1 mg

NBP2-49888

Immunocytochemistry/Immunofluorescence: Tat-Beclin 1 D11 Autophagy Inducing Peptide [NBP2-49888] - HeLa GFP-LC3B cells were treated with Tat-D11, Tat-L11, Tat-Beclin 1 or Tat-L11S for 1.5 hours. Thereafter, the cells were stained using NeuroTrace Red or DAPI and analyzed employing fluorescent microscopy. Note the higher number of autophagosomes/GFP-LC3B+ puncta in the images of Tat-D11 and Tat-L11 treated cells when compared to Tat-Beclin 1 and Tat-L11S treated cells.

In vitro assay: Tat-Beclin 1 D11 Autophagy Inducing Peptide - Retroinverso form [NBP2-49888] - Analysis of lysates from HeLa cells that were left untreated (blank) or were treated with 10-20 uM each of Tat-D11, Tat-L11, Tat-Beclin 1 or Tat-L11S. The lysates were analyzed for the expression of LC3-1/LC3-II and SQSTM1/p62 using 2 ug/mL each of anti-LC3B (NB100-2220) and anti-SQSTM1/p62 (MAB8028) respectively. Anti-Actin (AF4000) was used as a loading control. TatD11 exhibited superior induction of LC3-II and down-regulation of p62 protein when compared to other treatment and control groups.

In vivo assay: Tat-Beclin 1 D11 Autophagy Inducing Peptide - Retroinverso form [NBP2-49888] - In vivo dose study in 10wk old C57BL/6J mice. Either 1mg/kg or 10mg/kg IP once daily was administered for 2 days, mice were sacrificed, kidneys prepared for Western blot analysis. Lysosomal inhibitor bafilomycin A1 was used to provide a measurement of autophagic flux. *vehicle is scrambled tat-beclin (NBP2-49887-5mg). Image from verified customer review.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

Applications:

Sizes:

Sample Size Available

Sizes: Sample Size Available

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