GFP Antibody (9F9.F9)

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T., . . . Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S., . . . Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 13 applications: Cytometric Bead Assay Standard, ELISA, IB, ICC/IF, IF/IHC, IHC-Fr, IHC-P, IP, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, In vitro, KD, WB.

Showing Publications 1 - 10 of 56. Show All 56 Publications. Collapse Publications.

Publications using NB600-597	Applications	Species
Facci L, Bolego C, Chemello C et al. 2-Pentadecyl-2-oxazoline inhibits lipopolysaccharide-induced microglia activation interfering with TLR4 signaling Life sciences 2023-12-15 [PMID: 37952834]
Essandoh K, Subramani A, Ferro OA et al. zDHHC9 Regulates Cardiomyocyte Rab3a Activity and Atrial Natriuretic Peptide Secretion Through Palmitoylation of Rab3gap1 JACC: Basic to Translational Science 2023-05-01 [PMID: 37325411] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence)	Immunohistochemistry, Immunocytochemistry/ Immunofluorescence
Phillips HL, Dai H, Choi SY et al. Dorsomedial prefrontal hypoexcitability underlies lost empathy in frontotemporal dementia Neuron 2023-01-09 [PMID: 36638803] (IHC-Fr) Details: Dilution used 1:1000	IHC-Fr
Hartl I, Brumovska V, Striedner Y et al. Measurement of FGFR3 signaling at the cell membrane via total internal reflection fluorescence microscopy to compare the activation of FGFR3 mutants The Journal of biological chemistry 2022-12-26 [PMID: 36581204]
Dwyer ME, Hangarter RP. Light-induced displacement of PLASTID MOVEMENT IMPAIRED1 precedes light-dependent chloroplast movements Plant Physiology 2022-06-27 [PMID: 35477788]
Dwyer ME, Hangarter RP. Light-dependent phosphorylation of THRUMIN1 regulates its association with actin filaments and 14-3-3 proteins Plant Physiology 2021-11-03 [PMID: 34618069]
Houston R, Sekine Y, Larsen MB et al. Discovery of bactericides as an acute mitochondrial membrane damage inducer Molecular Biology of the Cell 2021-11-01 [PMID: 34495738] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Bowman SL, Le L, Zhu Y et al. A BLOC-1-AP-3 super-complex sorts a cis-SNARE complex into endosome-derived tubular transport carriers The Journal of cell biology 2021-07-05 [PMID: 33886957]
Bernadzki KM, Daszczuk P, Rojek KO et al. Arhgef5 Binds alpha-Dystrobrevin 1 and Regulates Neuromuscular Junction Integrity Front Mol Neurosci 2020-06-10 [PMID: 32587503] (WB)	WB
Wolf EJ, Miles A, Lee ES et al. MKRN2 Physically Interacts with GLE1 to Regulate mRNA Export and Zebrafish Retinal Development Cell Rep 2020-05-26 [PMID: 32460013] (WB)	WB
Pottinger SE, Bak A, Margets A et al. Optimizing the PBS1 Decoy System to Confer Resistance to Potyvirus Infection in Arabidopsis and Soybean Mol Plant Microbe Interact 2020-04-09 [PMID: 32267815]
Redditt TJ, Chung EH, Karimi HZ et al. AvrRpm1 Functions as an ADP-Ribosyl Transferase to Modify NOI Domain-Containing Proteins, Including Arabidopsis and Soybean RPM1-Interacting Protein4 Plant Cell 2019-11-16 [PMID: 31727786] (IB)	IB
Higuchi C, Yamamoto M, Shin SW et al. Perturbation of maternal PIASy abundance disrupts zygotic genome activation and embryonic development via SUMOylation pathway Biol Open 2019-10-24 [PMID: 31640975] (WB)	WB
Redditt TJ, Chung EH, Zand Karimi H et al. AvrRpm1 Functions as an ADP-Ribosyl Transferase to Modify NOI-domain Containing Proteins, Including Arabidopsis and Soybean RPM1-interacting Protein 4 Plant Cell 2019-09-25 [PMID: 31548257] (WB)	WB
Zusso M, Lunardi V, Franceschini D et al. Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway J Neuroinflammation 2019-07-18 [PMID: 31319868] (IP, WB)	IP, WB
Carter M, Helm M, Chapman A et al. Convergent evolution of effector protease recognition by Arabidopsis and barley. bioRxiv 2018-07-23 [PMID: 30480480]
Methot L, Soubannier V, Hermann R et al. Nuclear factor-kappaB regulates multiple steps of gliogenesis in the developing murine cerebral cortex Glia 2018-10-20 [PMID: 30338559] (ICC/IF)	ICC/IF
Dumon C, Diabira D, Chudotvorova I et al. The adipocyte hormone leptin sets the emergence of hippocampal inhibition in mice Elife 2018-08-15 [PMID: 30106375] (ICC/IF)	ICC/IF
Wolf EJ MKRN2 Interacts with GLE1 and Negatively Regulates mRNA Nuclear Export and Retinal Development Thesis (WB, KD, IP, Human)	WB, KD, IP	Human
Ping S, Qiu X, Gonzalez-Toledo ME et al. Stem Cell Factor in Combination with Granulocyte Colony-Stimulating Factor reduces Cerebral Capillary Thrombosis in a Mouse Model of CADASIL Cell Transplant 2018-06-07 [PMID: 29871518] (IF/IHC)	IF/IHC
Essandoh K, Subramani A, Ferro O et al. zDHHC9 Regulates Cardiomyocyte Rab3a Activity and Atrial Natriuretic Peptide Secretion Through Palmitoylation of Rab3gap1 JACC: Basic to Translational Science 2023-02-01 (WB)	WB
Fulop G, Brameshuber M, Arnold AM et al. Determination of the Membrane Environment of CD59 in Living Cells Biomolecules May 17 2018 [PMID: 29772810] Biomolecules. 2018-05-17 [PMID: 29772810] Details: Citation using the Biotin form of this antibody.
Bocchi R, Egervari K, Carol-Perdiguer L et al. Perturbed Wnt signaling leads to neuronal migration delay, altered interhemispheric connections and impaired social behavior Nat Commun 2017-10-29 [PMID: 29079819]
Vierra NC, Dadi PK, Milian SC et al. TALK-1 channels control b cell endoplasmic reticulum Ca(2+) homeostasis. Sci Signal. 2017-09-19 [PMID: 28928238]
Zusso M, Mercanti G, Belluti F et al. Phenolic 1,3-diketones attenuate lipopolysaccharide-induced inflammatory response by an alternative magnesium-mediated mechanism Br J Pharmacol 2017-02-16 [PMID: 28198010] (IP)	IP
Barbara Schlingmann, Christian E. Overgaard, Samuel A. Molina, K. Sabrina Lynn, Leslie A. Mitchell, StevenClaude Dorsainvil White, Alexa L. Mattheyses, David M. Guidot, Christopher T. Capaldo, Michael Koval Regulation of claudin/zonula occludens-1 complexes by hetero-claudin interactions Nature Communications 2016-07-25 [PMID: 27452368]
Baumgart F, Arnold AM, Leskovar K et al. Varying label density allows artifact-free analysis of membrane-protein nanoclusters Nat Methods. 2016-08-01 [PMID: 27295310] Details: Citation used the Biotin format of this antibody.
Song JM, Sung YM, Nam JH et al. A Mercaptoacetamide-Based Class II Histone Deacetylase Inhibitor Increases Dendritic Spine Density via RasGRF1/ERK Pathway J. Alzheimers Dis. 2016-02-03 [PMID: 26890742]
Boltengagen M, Huang A, Boltengagen A et al. A novel role for the histone acetyltransferase Hat1 in the CENP-A/CID assembly pathway in Drosophila melanogaster Nucleic Acids Res 2015-11-21 [PMID: 26586808] (ICC/IF)	ICC/IF
Kim M, Jeong Y, Chang YC. Extracellular matrix protein reelin regulate dendritic spine density through CaMKIIbeta. Neurosci Lett 2015-07-10 [PMID: 26003447]
Della Pia EA, Martinez KL. Single domain antibodies as a powerful tool for high quality surface plasmon resonance studies PLoS ONE 2015-03-31 [PMID: 25822527] Details: This citation used the Biotin version of this antibody.
Bin NR, Jung CH, Kim B et al. Chaperoning of closed syntaxin-3 through Lys46 and Glu59 in domain 1 of Munc18 proteins is indispensable for mast cell exocytosis J Cell Sci 2015-03-22 [PMID: 25795302] (ICC/IF)	ICC/IF
Pelassa I, Cora D, Cesano F et al. Association of polyalanine and polyglutamine coiled coils mediates expansion disease-related protein aggregation and dysfunction Hum Mol Genet 2014-02-06 [PMID: 24497578] (Cytometric Bead Assay Standard)	Cytometric Bead Assay Standard
Ding Q, Miyazaki Y, Tsukasa K et al. CD133 facilitates epithelial-mesenchymal transition through interaction with the ERK pathway in pancreatic cancer metastasis. Mol. Cancer 2014-02-24 [PMID: 24468059] (IHC-P)	IHC-P
Rieusset A, Schaller F, Unmehopa U et al. Stochastic loss of silencing of the imprinted Ndn/NDN allele, in a mouse model and humans with prader-willi syndrome, has functional consequences. PLoS Genet 2013-01-01 [PMID: 24039599]
Sohn YI, Lee NJ, Chung A et al. Antihypertensive drug Valsartan promotes dendritic spine density by altering AMPA receptor trafficking. Biochem Biophys Res Commun. 2013-10-04 [PMID: 24012668] (ICC/IF)	ICC/IF
Megill A, Lee T, DiBattista AM et al. A tetra(ethylene glycol) derivative of benzothiazole aniline enhances Ras-mediated spinogenesis J Neurosci 2013-05-31 [PMID: 23719799] (ICC/IF)	ICC/IF
Sunzenauer S, Zojer V, Brameshuber M et al. Determination of binding curves via protein micropatterning in vitro and in living cells. Cytometry A. 2013-09-01 [PMID: 23125142] (In vitro)	In vitro
Zusso M, Methot L, Lo R et al. Regulation of postnatal forebrain amoeboid microglial cell proliferation and development by the transcription factor Runx1 J Neurosci 2012-08-17 [PMID: 22895712] (ICC/IF)	ICC/IF
Muhia M, Willadt S et al. Molecular and behavioral changes associated with adult hippocampus-specific SynGAP1 knockout. Learn Mem 2012-06-16 [PMID: 22700469] (IF/IHC)	IF/IHC
Wei T, Schubert T, Paquet-Durand F et al. Light-driven calcium signals in mouse cone photoreceptors J Neurosci 2012-05-18 [PMID: 22593066]
Jain S, Amiji M. Tuftsin-modified alginate nanoparticles as a noncondensing macrophage-targeted DNA delivery system Biomacromolecules 2012-03-06 [PMID: 22385328] (ELISA)	ELISA
Attarwala H, Amiji M. Multi-compartmental nanoparticles-in-emulsion formulation for macrophage-specific anti-inflammatory gene delivery Pharm Res 2012-01-28 [PMID: 22281760] (ELISA)	ELISA
Xu J, Amiji M. Therapeutic gene delivery and transfection in human pancreatic cancer cells using epidermal growth factor receptor-targeted gelatin nanoparticles J Vis Exp 2012-01-04 [PMID: 22231028] (ELISA) Details: This citation used the Alkaline Phosphatase version of this antibody.	ELISA
Maira M, Long JE, Lee AY et al. Erratum to: Role for TGF-beta superfamily signaling in telencephalic GABAergic neuron development. J Neurodev Disord 2010-03-01 [PMID: 22127839]
Aebischer J, Sturny R, Andrieu D et al. Necdin protects embryonic motoneurons from programmed cell death. PLoS One 2011-01-01 [PMID: 21912643]
Goel M, Sienkiewicz AE, Picciani R et al. Cochlin Induced TREK-1 Co-Expression and Annexin A2 Secretion: Role in Trabecular Meshwork Cell Elongation and Motility. PLoS One 2011-01-01 [PMID: 21886777]
Damulewicz M, Pyza E. The clock input to the first optic neuropil of Drosophila melanogaster expressing neuronal circadian plasticity. PLoS One 2011-01-01 [PMID: 21760878]
Rolando C, Gribaudo S, Yoshikawa K et al. Extracerebellar progenitors grafted to the neurogenic milieu of the postnatal rat cerebellum adapt to the host environment but fail to acquire cerebellar identities Eur J Neurosci 2010-04-14 [PMID: 20384777]
Maira M, Long JE, Lee AY et al. Role for TGF-beta superfamily signaling in telencephalic GABAergic neuron development J Neurodev Disord 2010-03-27 [PMID: 20339443] (IF/IHC)	IF/IHC
Jesse S, Koenig A, Ellenrieder V et al. Lef-1 isoforms regulate different target genes and reduce cellular adhesion. Int J Cancer 2010-03-01 [PMID: 19653274]
Shen W, Da Silva JS, He H et al. Type A GABA-receptor-dependent synaptic transmission sculpts dendritic arbor structure in Xenopus tadpoles in vivo. J Neurosci 2009-04-01 [PMID: 19369572]
Yang F, Camp DG 2nd, Gritsenko MA et al. Identification of a novel mitotic phosphorylation motif associated with protein localization to the mitotic apparatus. J Cell Sci 2007-11-01 [PMID: 17971412]
Forrest JC, Paden CR, Allen RD 3rd et al. ORF73-null murine gammaherpesvirus 68 reveals roles for mLANA and p53 in virus replication. J Virol 2007-11-01 [PMID: 17699571]
Koenig A, Mueller C, Hasel C et al. Collagen type I induces disruption of E-cadherin-mediated cell-cell contacts and promotes proliferation of pancreatic carcinoma cells. Cancer Res. 2006-05-01 [PMID: 16651417]
Malagelada C, Jin ZH, Greene LA. RTP801 Is Induced in Parkinson's Disease Mediates Neuron Death by Inhibiting Akt Phosphorylation/Activation. J Neurosci;28(53):14363-14371. 2008-01-01 [PMID: 12923176]
Show All 56 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	4 reviewed by: Feng Li	WB	Human	11/18/2019	View Summary Application Western Blot Sample Tested 293 cell extract Species Human Lot 37157
Enlarge	5 reviewed by: Verified Customer	ICC	Human	05/10/2018	View Summary Application Immunocytochemistry Sample Tested HEK-293T cells transduced to have GFP Species Human Lot lot# 200ug/100ul Comments Comments The 293T cells have been transduced to have GFP which was detected by an antibody after permeabilization. The green staining shows the anti-GFP antibody.
Enlarge	5 reviewed by: Verified Customer	WB	Other	01/26/2015	View Summary Application Western Blot Sample Tested CHOK1 cells transfected with GFP Species Other Lot 28856

Array NB600-597

• GFP Antibodies
• GFP Proteins