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![Western Blot: NMDAR2B Antibody [NB300-106] - The blots show the examples of immunobands against NMDAR2B (C) and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (//www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Western-Blot-NB300-106-img0006.jpg "Western Blot: NMDAR2B Antibody [NB300-106] - The blots show the examples of immunobands against NMDAR2B (C) and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (//www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY license.")
![Flow Cytometry: NMDAR2B Antibody [NB300-106] - NMDA2B receptor expression on CD4+ T cells. Image supplied by review from confirmed customer.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Flow-Cytometry-NB300-106-img0004.jpg)
![Western Blot: NMDAR2B Antibody [NB300-106]](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Western-Blot-NB300-106-img0005.jpg)
![Western Blot: NMDAR2B Antibody (NR2B) [NB100-74475] - Analysis of NMDA receptor type 2B in mouse brain tissue.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-NR2B-Western-Blot-NB100-74475-img0003.jpg "Western Blot: NMDAR2B Antibody (NR2B) [NB100-74475] - Analysis of NMDA receptor type 2B in mouse brain tissue.")
![Immunohistochemistry-Paraffin: NMDAR2B Antibody (NR2B) [NB100-74475] - Analysis showing staining in the cytoplasm and membrane of human breast tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-NR2B-Immunohistochemistry-Paraffin-NB100-74475-img0004.jpg)
![Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NMDAR2B protein using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Western-Blot-NBP1-47611-img0008.jpg "Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NMDAR2B protein using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2B/NMDAR2B Antibody (D) Composite.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Immunocytochemistry-Immunofluorescence-NBP1-47611-img0010.jpg)
![Immunohistochemistry: NMDAR2B Antibody (S59) [NBP1-47611] - Immunohistochemistry analysis using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining, and dermal staining.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Immunohistochemistry-NBP1-47611-img0009.jpg)
![Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - analysis of Human HEK-T lysates showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36 . Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:250.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Western-Blot-NBP1-47611-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything positive.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Immunocytochemistry-Immunofluorescence-NBP1-47611-img0005.jpg)
![Western Blot: NMDAR2B Antibody [NB100-41097] - (2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Western-Blot-NB100-41097-img0003.jpg "Western Blot: NMDAR2B Antibody [NB100-41097] - (2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody [NB100-41097] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoskeleton and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41097-img0004.jpg)
![Western Blot: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis using GRIN2B mAb against human GRIN2B (AA: extra 27-163) recombinant protein. (Expected MW is 41 kDa)](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Western-Blot-NBP2-61746-img0005.jpg "Western Blot: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis using GRIN2B mAb against human GRIN2B (AA: extra 27-163) recombinant protein. (Expected MW is 41 kDa)")
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis of SK-N-SH cells using GRIN2B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Immunocytochemistry-Immunofluorescence-NBP2-61746-img0004.jpg)
![Flow Cytometry: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis of SK-N-SH cells using GRIN2B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Flow-Cytometry-NBP2-61746-img0002.jpg)
![Western Blot: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis using GRIN2B mAb against HEK293 (1) and GRIN2B (AA: extra 27-163)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Western-Blot-NBP2-61746-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis of Hela cells using GRIN2B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Immunocytochemistry-Immunofluorescence-NBP2-61746-img0003.jpg)
![ELISA: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-ELISA-NBP2-61746-img0001.jpg)
![Western Blot: NMDAR2B Antibody [NBP1-30037]](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Western-Blot-NBP1-30037-img0002.jpg "Western Blot: NMDAR2B Antibody [NBP1-30037]")
![Immunohistochemistry: NMDAR2B Antibody [NBP1-30037] - Immunostaining of 14 DIV rat cortical neurons showing NR2B in green and PSD95 in red.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-Immunohistochemistry-NBP1-30037-img0001.jpg)
![Western Blot: NMDAR2B [p Tyr1336] Antibody [NB300-295] - Analysis of Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR (Control). The phosphospecificty of this labeling is shown in the second lane (lambda-phosphatase: lambda Ptase). The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr1336 NMDA NR2B subunit. The immunolabeling of NR2B is completely eliminated by treatment with lambda Ptase.](http://images.novusbio.com/fullsize/NMDAR2B-[p-Tyr1336]-Antibody-Western-Blot-NB300-295-img0004.jpg "Western Blot: NMDAR2B [p Tyr1336] Antibody [NB300-295] - Analysis of Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR (Control). The phosphospecificty of this labeling is shown in the second lane (lambda-phosphatase: lambda Ptase). The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr1336 NMDA NR2B subunit. The immunolabeling of NR2B is completely eliminated by treatment with lambda Ptase.")
![Immunohistochemistry: NMDAR2B [p Tyr1336] Antibody [NB300-295] - Staining of Mouse dentate gyrus 48 hr post TMT treatment showing NR2B when phosphorylated at Tyr1336 in green and nuclei in blue.](http://images.novusbio.com/fullsize/NMDAR2B-[p-Tyr1336]-Antibody-Immunohistochemistry-NB300-295-img0002.jpg)
![Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103] - Changes of NMDAR2B phosphorylation levels in the hippocampus and prefrontal cortex of P28 mice after earlylife propofol exposure. Typical results of western blot showed the changes in pSer1480 NMDAR2B phosphorylation levels in cytosol and particulate fractions of the hippocampus and prefrontal cortex of P28 mice after earlylife propofol exposure. D6 mice group treated with propofol (100 mg/kg body weight, i.p.) daily P6-P11; D1 mice group treated with propofil (100 mg/kg, i.p.) at P6 and then normal saline (10 mL/kg, i.p.) from P7-P11; N mice group received normal saline (10 mL/kg, i.p.) from P6-P11. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28729940/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NMDAR2B-[p-Ser1480]-Antibody-Western-Blot-NB100-61103-img0002.jpg "Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103] - Changes of NMDAR2B phosphorylation levels in the hippocampus and prefrontal cortex of P28 mice after earlylife propofol exposure. Typical results of western blot showed the changes in pSer1480 NMDAR2B phosphorylation levels in cytosol and particulate fractions of the hippocampus and prefrontal cortex of P28 mice after earlylife propofol exposure. D6 mice group treated with propofol (100 mg/kg body weight, i.p.) daily P6-P11; D1 mice group treated with propofil (100 mg/kg, i.p.) at P6 and then normal saline (10 mL/kg, i.p.) from P7-P11; N mice group received normal saline (10 mL/kg, i.p.) from P6-P11. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28729940/) licensed under a CC-BY license.")
![Western Blot: NMDAR2B [p Ser1480] Antibody [NB100-61103] - Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Ser1480 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser1480 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/NMDAR2B-[p-Ser1480]-Antibody-Western-Blot-NB100-61103-img0001.jpg)
![Western Blot: NMDAR2B [p Tyr1472] Antibody [NB300-182] - Rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Tyr1472 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).](http://images.novusbio.com/fullsize/NMDAR2B-[p-Tyr1472]-Antibody-Western-Blot-NB300-182-img0002.jpg "Western Blot: NMDAR2B [p Tyr1472] Antibody [NB300-182] - Rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Tyr1472 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).")
![Western Blot: NMDAR2B [p Tyr1252] Antibody [NB300-292] - Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit phosphorylated at Tyr1252 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1252 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/NMDAR2B-[p-Tyr1252]-Antibody-Western-Blot-NB300-292-img0002.jpg "Western Blot: NMDAR2B [p Tyr1252] Antibody [NB300-292] - Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit phosphorylated at Tyr1252 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1252 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Immunohistochemistry: NMDAR2B [p Tyr1252] Antibody [NB300-292] - Immunostaining of 14 DIV rat cortical neurons showing NR2B phosphorylated at Tyr1252 in red and PSD95 in green. Photo courtesy of Gang Liu](http://images.novusbio.com/fullsize/NMDAR2B-[p-Tyr1252]-Antibody-Immunohistochemistry-NB300-292-img0003.jpg)
![Proximity Ligation Assay: NMDAR2B Antibody (2G5) [H00002904-M01] - Analysis of protein-protein interactions between CAPN1 and GRIN2B. HeLa cells were stained with anti-CAPN1 rabbit purified polyclonal 1:1200 and anti-GRIN2B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-2G5-Proximity-Ligation-Assay-H00002904-M01-img0002.jpg "Proximity Ligation Assay: NMDAR2B Antibody (2G5) [H00002904-M01] - Analysis of protein-protein interactions between CAPN1 and GRIN2B. HeLa cells were stained with anti-CAPN1 rabbit purified polyclonal 1:1200 and anti-GRIN2B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Sandwich ELISA: NMDAR2B Antibody (2G5) [H00002904-M01] - Detection limit for recombinant GST tagged GRIN2B is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-2G5-Sandwich-ELISA-H00002904-M01-img0003.jpg)
![Western Blot: NMDAR2B [p Ser1166] Antibody [NBP2-29520] - Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Ser1166 (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide), but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/NMDAR2B-[p-Ser1166]-Antibody-Western-Blot-NBP2-29520-img0001.jpg "Western Blot: NMDAR2B [p Ser1166] Antibody [NBP2-29520] - Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Ser1166 (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide), but not by the corresponding dephospho-peptide (not shown).")