| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1287SS | |||
| Azide and BSA Free | NBP2-80735 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-44306SS | |||
| Azide and BSA Free | NBP2-44309SS | |||
| Azide and BSA Free | NBP2-44309 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70653 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92947-0.02ml | |||
![Western Blot: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis of MCF7 (A), HepG2 (B), NIH3T3 (C), and PC-12 (D) cell lysate using FGF R1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M19B2-Western-Blot-NB600-1287-img0007.jpg "Western Blot: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis of MCF7 (A), HepG2 (B), NIH3T3 (C), and PC-12 (D) cell lysate using FGF R1 antibody at 2 ug/ml.")
![Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis using the Alexa Fluor 488 (R) conjugate of NB600-1287. Staining of FGF R1 in HEK293 and HEK293 FGFR transfected cells using anti-FGF R1 antibody. (Red) Positive cells, (Green) Negative cells, (Blue) Negative cells + antibody, (Pink) Positive cells + antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M19B2-Flow-Cytometry-NB600-1287-img0009.jpg)
![Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using FGF R1 antibody (Dark Blue) and Isotype control (Orange). An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M19B2-Flow-Cytometry-NB600-1287-img0005.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining in human skin and skeletal muscle tissues . Corresponding CD44 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining in human skin and skeletal muscle tissues . Corresponding CD44 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD44 Antibody [NBP1-84578] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/CD44-Antibody-Western-Blot-NBP1-84578-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP1-84578] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84578-img0022.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human lymph node shows strong positivity in plasma membrane in non-germinal center cells.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0023.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0018.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human squamous cell lung carcinoma shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0019.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP1-84578] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP1-84578-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HGFR/c-MET Antibody [1G7NB] (NBP2-44306) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HGFR-c-MET-Antibody-1G7NB-Immunocytochemistry-Immunofluorescence-NBP2-44306-img0007.jpg "Immunocytochemistry/Immunofluorescence: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HGFR/c-MET Antibody [1G7NB] (NBP2-44306) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - Analysis of HGF R/c-Met (1G7NB) in human liver cancer.](http://images.novusbio.com/fullsize/HGFR-c-MET-Antibody-1G7NB-Immunohistochemistry-Paraffin-NBP2-44306-img0001.jpg)
![Flow Cytometry: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - c-MET antibody was tested in HeLa cells (1 x 10^6 cells/ml). After fixation and permeabilization, cells were stained using the anti-c-MET antibody (clone 1G7NB) at a 1:1000 dilution. Signal was detected using a Goat anti-Mouse Dylight 488 secondary (blue peak). Shown with secondary control (orange peak). Data was acquired on BD FACSCalibur. Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/HGFR-c-MET-Antibody-1G7NB-Flow-Cytometry-NBP2-44306-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HGFR/c-MET Antibody (1G7NB) [NBP2-44306] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with c-MET (1G7NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green). Actin was detected using Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HGFR-c-MET-Antibody-1G7NB-Immunocytochemistry-Immunofluorescence-NBP2-44306-img0003.jpg)
![Western Blot: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of PIM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: BT-20 cell lysateLane 2: HepG2 cell lysate Lane 3: SW480 cell lysate](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Western-Blot-NBP2-67528-img0010.jpg "Western Blot: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of PIM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: BT-20 cell lysateLane 2: HepG2 cell lysate Lane 3: SW480 cell lysate")
![Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunocytochemistry-Immunofluorescence-NBP2-67528-img0009.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human tonsil tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunocytochemistry-Immunofluorescence-NBP2-67528-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PIM1 Antibody (ST0513) [NBP2-67528] - Staining PIM1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunocytochemistry-Immunofluorescence-NBP2-67528-img0008.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human breast carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0002.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human colon cancer tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0003.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human gastric carcinoma tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0004.jpg)
![Immunohistochemistry-Paraffin: PIM1 Antibody (ST0513) [NBP2-67528] - Analysis of human spleen tissue using anti-PIM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PIM1-Antibody-ST0513-Immunohistochemistry-Paraffin-NBP2-67528-img0005.jpg)
![Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Lane 1: HL-60 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: Jurkat cell lysateLysates/proteins at 10 ug/Lane. Predicted band size: 26 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Western-Blot-NBP2-67182-img0010.jpg "Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Lane 1: HL-60 cell lysate, Lane 2: THP-1 cell lysate, Lane 3: Jurkat cell lysateLysates/proteins at 10 ug/Lane. Predicted band size: 26 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Staining Bcl-2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunocytochemistry-Immunofluorescence-NBP2-67182-img0003.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded mouse kidney tissue using anti-Bcl-2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0011.jpg)
![Flow Cytometry: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of Jurkat cells with Bcl-2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Flow-Cytometry-NBP2-67182-img0001.jpg)
![Western Blot: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Western blot analysis of Bcl-2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF-7 cell lysate](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Western-Blot-NBP2-67182-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Staining Bcl-2 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunocytochemistry-Immunofluorescence-NBP2-67182-img0002.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human colon cancer tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0004.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human lung cancer tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0005.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded human tonsil tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0006.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (JF104-8) [NBP2-67182] - Analysis of paraffin-embedded mouse kidney tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-Antibody-JF104-8-Immunohistochemistry-Paraffin-NBP2-67182-img0007.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16842] - HIST1H3A antibody detects HIST1H3A protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody diluted at a dilution of 1:1000.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Western-Blot-NBP2-16842-img0019.jpg "Western Blot: HIST1H3A Antibody [NBP2-16842] - HIST1H3A antibody detects HIST1H3A protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody diluted at a dilution of 1:1000.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16842] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: Histone H3 protein stained by Histone H3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16842-img0007.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Green: Histone H3 antibody diluted at 1:500. Red: alpha Tubulin antibody [114] (NBP2-43837) diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0018.jpg)
![Chromatin Immunoprecipitation: HIST1H3A Antibody [NBP2-16842] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-Histone H3 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-16842-img0005.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16842] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Western-Blot-NBP2-16842-img0013.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16842] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Western-Blot-NBP2-16842-img0015.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0002.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Paraffin-embedded mouse colon. Histone H3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16842] - Paraffin-embedded mouse brain. Histone H3 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16842-img0004.jpg)
![Immunoprecipitation: HIST1H3A Antibody [NBP2-16842] - IP Sample: Raji whole cell lysate/extract A : Control with 3 ug of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 ug of Histone H3 antibody15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunoprecipitation-NBP2-16842-img0009.jpg)
![Western Blot: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Western-Blot-NBP2-43535-img0010.jpg "Western Blot: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K18ac (Acetyl Lys18) antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 (acetyl Lys18) protein stained by Histone H3 (acetyl Lys18) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-43535-img0003.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of rat fore brain. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0007.jpg)
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H3K18ac (acetyl Lys18) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or beta-globin promoter.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Chromatin-Immunoprecipitation-NBP2-43535-img0001.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse duodenum. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse intestine. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0005.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of mouse prostate. Histone H3K18ac (acetyl Lys18) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunohistochemistry-Paraffin-NBP2-43535-img0006.jpg)
![Dot Blot: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of anti-Histone H3 (acetyl Lys18) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys18) antibody 1:5000 dilution. A: Peptide samples of H3K18 un B: Peptide samples of H3K18ac C: Peptide samples of H3K9un D: Peptide samples of H3K9ac E: Peptide samples of H3K14un F: Peptide samples of H3K14ac G: Peptide samples of H3K27un H: Peptide samples of H3K27ac .](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Dot-Blot-NBP2-43535-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: HIST1H3A [ac Lys18] Antibody [NBP2-43535] - Analysis of HeLa whole cell lysate/extract: A. 30 ug whole cell lysate/extract of Histone H3 (acetyl Lys18) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B. Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of Histone H3 (acetyl Lys18) protein by 2.5 ug of Histone H3 (acetyl Lys18) antibody 12% SDS-PAGE The immunoprecipitated Histone H3 protein (acetyl Lys18) was detected by Histone H3 (acetyl Lys18) antibody. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-[ac-Lys18]-Antibody-Immunoprecipitation-NBP2-43535-img0008.jpg)
![Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152] - Analysis of extracts (35ug) from 2 different Human cell lines, dilution (1:500).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-OTI4F10-Western-Blot-NBP2-01152-img0010.jpg "Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152] - Analysis of extracts (35ug) from 2 different Human cell lines, dilution (1:500).")
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ErbB2/HER2.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-4F10-Immunocytochemistry-Immunofluorescence-NBP2-01152-img0008.jpg)
![Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Human Kidney tissue using anti-ErbB2/HER2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Immunohistochemistry-Paraffin-NBP2-01152-img0005.jpg)
![Flow Cytometry: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ErbB2/HER2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Flow-Cytometry-NBP2-01152-img0001.jpg)
![Western Blot: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ErbB2/HER2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ErbB2/HER2.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Western-Blot-NBP2-01152-img0002.jpg)
![Western Blot: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - 1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8:Colon.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Western-Blot-NBP2-01152-img0007.jpg)
![Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ErbB2/HER2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Immunohistochemistry-Paraffin-NBP2-01152-img0003.jpg)
![Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ErbB2/HER2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Immunohistochemistry-Paraffin-NBP2-01152-img0004.jpg)
![Western Blot: ErbB2/Her2 Antibody (OTI4F10) - Azide and BSA Free [NBP2-70653] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ErbB2/HER2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ErbB2/HER2.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-OTI4F10-Azide-and-BSA-Free-Western-Blot-NBP2-70653-img0003.jpg "Western Blot: ErbB2/Her2 Antibody (OTI4F10) - Azide and BSA Free [NBP2-70653] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ErbB2/HER2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ErbB2/HER2.")
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (OTI4F10) - Azide and BSA Free [NBP2-70653] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ErbB2/HER2.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-OTI4F10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70653-img0005.jpg)
![Immunohistochemistry: ErbB2/Her2 Antibody (OTI4F10) - Azide and BSA Free [NBP2-70653] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ErbB2/HER2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-OTI4F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70653-img0007.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (OTI4F10) - Azide and BSA Free [NBP2-70653] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-ErbB2/HER2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-OTI4F10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70653-img0004.jpg)
![Western Blot: ErbB2/Her2 Antibody (OTI4F10) - Azide and BSA Free [NBP2-70653] - Analysis of extracts (35ug) from 2 different Human cell lines, dilution (1:500).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-OTI4F10-Azide-and-BSA-Free-Western-Blot-NBP2-70653-img0001.jpg)
![Western Blot: ErbB2/Her2 Antibody (OTI4F10) - Azide and BSA Free [NBP2-70653] - 1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8:Colon.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-OTI4F10-Azide-and-BSA-Free-Western-Blot-NBP2-70653-img0002.jpg)
![Immunohistochemistry: ErbB2/Her2 Antibody (OTI4F10) - Azide and BSA Free [NBP2-70653] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ErbB2/HER2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-OTI4F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70653-img0006.jpg)
![Immunohistochemistry: ErbB2/Her2 Antibody (OTI4F10) - Azide and BSA Free [NBP2-70653] - Staining of paraffin-embedded Human Kidney tissue using anti-ErbB2/HER2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-OTI4F10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70653-img0008.jpg)
![Western Blot: ErbB2/Her2 Antibody [NBP1-84584] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Western-Blot-NBP1-84584-img0006.jpg "Western Blot: ErbB2/Her2 Antibody [NBP1-84584] - Analysis in human cell line SK-BR-3.")
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human cell line MCF7 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84584-img0008.jpg)
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP1-84584-img0013.jpg)
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human breast cancer shows no positivity in tumor cells as expected.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP1-84584-img0010.jpg)
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human breast cancer shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP1-84584-img0011.jpg)
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human kidney shows weak to moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP1-84584-img0012.jpg)
![Western Blot: CD44 Antibody [NBP2-92947] - Analysis of extracts of various cell lines, using CD44 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/CD44-Antibody-Western-Blot-NBP2-92947-img0005.jpg "Western Blot: CD44 Antibody [NBP2-92947] - Analysis of extracts of various cell lines, using CD44 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP2-92947] - Analysis of RAW264.7 cells using CD44 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92947-img0004.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP2-92947] - Human lung cancer using CD44 Rabbit pAb at dilution of 1:250 (40x lens).](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP2-92947-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP2-92947] - Analysis of A431 cells using CD44 at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92947-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP2-92947] - Analysis of HeLa cells using CD44 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92947-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody [NBP2-92947] - Analysis of HeLa cells using CD44 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CD44-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92947-img0003.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody [NBP2-92947] - Human esophageal cancer using CD44 Rabbit pAb at dilution of 1:250 (40x lens).](http://images.novusbio.com/fullsize/CD44-Antibody-Immunohistochemistry-Paraffin-NBP2-92947-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: CD44 Antibody [NBP2-92947] - Analysis of extracts of HeLa cells, using CD44 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/CD44-Antibody-Knockout-Validated-NBP2-92947-img0006.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16838] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Western-Blot-NBP2-16838-img0010.jpg "Western Blot: HIST1H3A Antibody [NBP2-16838] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with HIST1H3A antibody diluted at 1:5000.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16838] - HIST1H3A antibody detects Histone HIST1H3A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIST1H3A protein stained by HIST1H3A antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16838-img0008.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A Antibody [NBP2-16838] - Immunohistochemical analysis of paraffin-embedded Lung AdCA, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunohistochemistry-Paraffin-NBP2-16838-img0002.jpg)
![Chromatin Immunoprecipitation (ChIP): HIST1H3A Antibody [NBP2-16838] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-HIST1H3A antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or beta-globin promoter.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-16838-img0009.jpg)
![Western Blot: HIST1H3A Antibody [NBP2-16838] - Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Western-Blot-NBP2-16838-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16838] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16838-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A Antibody [NBP2-16838] - HIST1H3A Antibody detects HIST1H3A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIST1H3A protein stained by HIST1H3A Antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16838-img0007.jpg)
![Chromatin Immunoprecipitation: HIST1H3A Antibody [NBP2-16838] - Histone H3 (di-methyl Lys4) antibody immunoprecipitates Histone H3 (di-methyl Lys4) protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 ug preimmune rabbit IgG B. 5 ug of Histone H3 (di-methyl Lys4) antibody The precipitated DNA was detected by PCR with primer set targeting to GAPDH gene locus. .](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Chromatin-Immunoprecipitation-NBP2-16838-img0006.jpg)
![Immunoprecipitation: HIST1H3A Antibody [NBP2-16838] - Sample: 1000 ug HeLa whole cell lysate/extract A. 50 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of Histone H3 (di-methyl Lys4) protein by 2 ug of Histone H3 (di-methyl Lys4) antibody 15% SDS-PAGE The immunoprecipitated Histone H3 (di-methyl Lys4) protein was detected by Histone H3 (di-methyl Lys4) antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HIST1H3A-Antibody-Immunoprecipitation-NBP2-16838-img0003.jpg)