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7 Results for "novus"

NBP1-87758

NFkB p105/p50 Antibody

(2 Publications)

0.1 ml

NBP1-87758

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining in human lymph node and cerebral cortex tissues using NBP1-87758 antibody. Corresponding NFKB1 RNA-seq data are presented for the same tissues.

<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: NFkB p105/p50 Antibody [NBP1-87758] - Analysis in SK-BR-3 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NFKB1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.

Western Blot: NFkB p105/p50 Antibody [NBP1-87758] - Analysis in human cell line Daudi.

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human cerebral cortex shows no positivity in neurons as expected.

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human colon shows moderate cytoplasmic positivity in glandular and lymphoid cells.

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human lymph node shows strong cytoplasmic positivity in germinal- and non-germinal center cells.

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-33061

DDB1 Antibody

(1 Publication)

0.1 ml

NBP1-33061

Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: DDB1 Antibody [NBP1-33061] - Analysis of paraformaldehyde-fixed HeLa.

Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Mouse brain. DDB1 stained by DDB1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: DDB1 Antibody [NBP1-33061] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of DDB1 protein by 2. 5 ug of DDB1 antibody 7. 5% SDS-PAGE gel.

Western Blot: DDB1 Antibody [NBP1-33061] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:5000.

Western Blot: DDB1 Antibody [NBP1-33061] - Sample (30 ug of whole cell lysate) NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: DDB1 Antibody [NBP1-33061] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000.

Western Blot: DDB1 Antibody [NBP1-33061] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DDB1 antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Cal27 Xenograft , using DDB1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Mouse prostate. DDB1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: DDB1 Antibody [NBP1-33061] - Rat brain. DDB1 stained by DDB1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

<b>Genetic Strategies Validation. </b>Knockdown Validated: DDB1 Antibody [NBP1-33061] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody ).

Reactivity:

Hu, Mu, Rt, Bv, Ch, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ch, Xp

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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H00002965-M01

GTF2H1 Antibody (1F12-1B5)

(2 Publications)

0.1 mg

H00002965-M01

Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody (M01), clone 1F12-1B5.Lane 1: GTF2H1 transfected lysate(62 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.Antibody concentration 5 ug/ml.

Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of GTF2H1 over-expressed 293 cell line, cotransfected with GTF2H1 Validated Chimera RNAi ( Cat # H00002965-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 (Cat # H00002965-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - GTF2H1 monoclonal antibody (M01), clone 1F12-1B5. Analysis of GTF2H1 expression in Hela S3 NE.

Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 Analysis of GTF2H1 expression in Jurkat.

Sandwich ELISA: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Detection limit for recombinant GST tagged GTF2H1 is approximately 1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-34480

ERCC1 Antibody (CL1284)

0.1 ml

NBP2-34480

Western Blot: ERCC1 Antibody (CL1284) [NBP2-34480] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ERCC1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

<b>Genetic Strategies Validation. </b>Western Blot: ERCC1 Antibody (CL1284) [NBP2-34480] - Lane 1: Marker [kDa] Lane 2: Human cell line RT-4

Immunohistochemistry-Paraffin: ERCC1 Antibody (CL1284) [NBP2-34480] - Staining of human colon shows nuclear immunoreactivity in both glandular and lymphoid cells.

Immunohistochemistry-Paraffin: ERCC1 Antibody (CL1284) [NBP2-34480] - Staining of human colorectal cancer shows strong nuclear immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: ERCC1 Antibody (CL1284) [NBP2-34480] - Staining of human ventricular cancer shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: ERCC1 Antibody (CL1284) [NBP2-34480] - Staining of human prostate cancer shows strong nuclear immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: ERCC1 Antibody (CL1284) [NBP2-34480] - Staining of human testis shows strong nuclear positivity in seminiferous tubules.

Simple Western: ERCC1 Antibody (CL1284) [NBP2-34480] - Simple Western lane view shows a specific band for ADARB1 in 0.2 mg/ml of RT-4, U-251MG sp lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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H00005886-M01

Rad23 Antibody (3C12)

(4 Publications)

0.1 mg

H00005886-M01

Western Blot: Rad23 Antibody (3C12) [H00005886-M01] - Analysis of RAD23A over-expressed 293 cell line, cotransfected with RAD23A Validated Chimera RNAi ( Cat # H00005886-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD23A monoclonal antibody (M01), clone 3C12 (Cat # H00005886-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: Rad23 Antibody (3C12) [H00005886-M01] - Analysis of RAD23A expression in transfected 293T cell line by RAD23A monoclonal antibody (M01), clone 3C12. Lane 1: RAD23A transfected lysate (39.6 KDa). Lane 2: Non-transfected lysate.

Sandwich ELISA: Rad23 Antibody (3C12) [H00005886-M01] - Detection limit for recombinant GST tagged RAD23A is approximately 0.03ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00006119-M01

RPA14 Antibody (1F4)

(1 Publication)

0.1 mg

H00006119-M01

Western Blot: RPA14 Antibody (1F4) [H00006119-M01] - Analysis of RPA3 expression in transfected 293T cell line by RPA3 monoclonal antibody (M01), clone 1F4.Lane 1: RPA3 transfected lysate(13.6 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: RPA14 Antibody (1F4) [H00006119-M01] - Analysis of monoclonal antibody to RPA3 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.

Western Blot: RPA14 Antibody (1F4) [H00006119-M01] - Analysis of RPA3 over-expressed 293 cell line, cotransfected with RPA3 Validated Chimera RNAi ( Cat # H00006119-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RPA3 monoclonal antibody (M01), clone 1F4 (Cat # H00006119-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: RPA14 Antibody (1F4) [H00006119-M01] - RPA3 monoclonal antibody (M01), clone 1F4 Analysis of RPA3 expression in HL-60.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NB100-332V

RPA2 Antibody [DyLight 405]

0.1 ml

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

DyLight 405

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-332AF350
Alexa Fluor 405	NB100-332AF405
Alexa Fluor 488	NB100-332AF488
Alexa Fluor 532	NB100-332AF532
Alexa Fluor 594	NB100-332AF594
Alexa Fluor 647	NB100-332AF647
Alexa Fluor 700	NB100-332AF700
Alexa Fluor 750	NB100-332AF750
Biotin	NB100-332B
DyLight 350	NB100-332UV
DyLight 488	NB100-332G
DyLight 550	NB100-332R
DyLight 594	NB100-332DL594
DyLight 650	NB100-332C
DyLight 680	NB100-332FR
DyLight 755	NB100-332IR
FITC	NB100-332F
HRP	NB100-332H
Janelia Fluor 549	NB100-332JF549
Janelia Fluor 646	NB100-332JF646

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