| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | BC100-494SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56503SS | |||
| Azide and BSA Free | NBP2-80686 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49678SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36488SS | |||
| Azide and BSA Free | NBP2-80910 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51841SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-66383SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71775SS | |||
| Azide and BSA Free | NBP2-80850 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-07047SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77397SS | |||
![Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Metformin activates apoptotic cell death; Right panel: MCF-7 cells were kept in the presence or absence of metformin for 20 days and then processed to obtain mitochondrial or whole cellular extracts. BAX and CYCS expression levels were determined by Western blot as indicated under Material and Methods. Densitometric analysis of the gels was performed as indicated under Material and Methods. PHB and CDK4 were used as purity and loading controls. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/1/49), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Western-Blot-NB100-56503-img0015.jpg "<b>Biological Strategies Validation. </b>Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Metformin activates apoptotic cell death; Right panel: MCF-7 cells were kept in the presence or absence of metformin for 20 days and then processed to obtain mitochondrial or whole cellular extracts. BAX and CYCS expression levels were determined by Western blot as indicated under Material and Methods. Densitometric analysis of the gels was performed as indicated under Material and Methods. PHB and CDK4 were used as purity and loading controls. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/1/49), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - p73 was detected in immersion fixed Hela human cell line using NB100-56503 at 25 ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLights(TM) 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Staining was observed in the cytoplasm and mitochondria.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Immunocytochemistry-Immunofluorescence-NB100-56503-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Cytochrome C was detected in immersion fixed paraffin-embedded sections of human heart using anti-human mouse monoclonal antibody (Catalog # NB100-56503) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Immunohistochemistry-Paraffin-NB100-56503-img0004.jpg)
![Flow (Intracellular): Cytochrome c Antibody (7H8.2C12) [NB100-56503] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Flow-Intracellular-NB100-56503-img0014.jpg)
![Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Human HeLa lysate probed with Cytochrome C antibody at 0.1 ug/ml.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Western-Blot-NB100-56503-img0001.jpg)
![Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Analysis using the biotin conjugate of NB100-56503. Detection of Cytochrome C in 15 ug of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is detected.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Western-Blot-NB100-56503-img0003.jpg)
![Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Detection of Cytochrome C in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Cytochrome C Monoclonal Antibody (Catalog # NB100-56503, filled histogram), or Mouse IgG2B isotype control (Catalog # MAB0041, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Flow-Cytometry-NB100-56503-img0005.jpg)
![Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Using the Alexa Fluor 488 direct conjugate, an intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Flow-Cytometry-NB100-56503-img0011.jpg)
![Simple Western: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytochrome c at approximately 23 kDa (as indicated) using 2.5 ug/mL of Mouse Anti-Cytochrome c Monoclonal Antibody (Catalog # NB100-56503). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-7H8-2C12-Simple-Western-NB100-56503-img0013.jpg)
![Simple Western: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Cytochrome C Antibodies (NB100-56503SS), ProteinSimple Western Blot on Jess Instrument; 1 microgram of human brain tissue lysate was tested with the antibodies diluted 20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-56503_mouse-monoclonal-cytochrome-c-antibody-7h8-2c12-imgenex-img-101a-simple-western-204202312023.jpg)
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NBP1-49678] - PINK1 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49678-img0002.jpg "Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NBP1-49678] - PINK1 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Simple Western: PINK1 Antibody [NBP1-49678] - Lane view shows a specific band for PINK1 at a dilution of 1:50 in 1.0 mg/ml of HeLa lysate. Molecular weight ~61kDa. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.](http://images.novusbio.com/fullsize/PINK1-Antibody-Simple-Western-NBP1-49678-img0006.jpg)
![Immunohistochemistry-Paraffin: PINK1 Antibody [NBP1-49678] - Stain in paraffin embedded mouse brain.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunohistochemistry-Paraffin-NBP1-49678-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NBP1-49678] - PINK1 antibody was tested at 1:50 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49678-img0005.jpg)
![Knockdown Validated: PINK1 Antibody (8E10.1D6) [NBP2-36488] - PINK1 is responsible for CMR-induced cytoplasmic vacuolation.The effect of siRNA-mediated knockdown of PINK1 on CMR-induced changes in the expression of Alix in MDA-MB-231 cells as analyzed by Western blot (left panel) All cells were treated with 6 uM CMR for 48 h. Image collected and cropped by Citeab from the following publication (Chalcomoracin is a potent anticancer agent acting through triggering Oxidative stress via a mitophagy- and paraptosis-dependent mechanism. Sci Rep (2018) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PINK1-Antibody-8E10-1D6-Western-Blot-NBP2-36488-img0007.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: PINK1 Antibody (8E10.1D6) [NBP2-36488] - PINK1 is responsible for CMR-induced cytoplasmic vacuolation.The effect of siRNA-mediated knockdown of PINK1 on CMR-induced changes in the expression of Alix in MDA-MB-231 cells as analyzed by Western blot (left panel) All cells were treated with 6 uM CMR for 48 h. Image collected and cropped by Citeab from the following publication (Chalcomoracin is a potent anticancer agent acting through triggering Oxidative stress via a mitophagy- and paraptosis-dependent mechanism. Sci Rep (2018) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: PINK1 Antibody (8E10.1D6) [NBP2-36488] - Analysis of FFPE tissue section of human hepatocellular carcinoma using PINK1 antibody (clone 8E10.1D6) at 5 ug/ml concentration. The cancer cells developed an expected punctate to granular cytoplasmic staining with no signal in the tumor stroma.](http://images.novusbio.com/fullsize/PINK1-Antibody-8E10-1D6-Immunohistochemistry-Paraffin-NBP2-36488-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PINK1 Antibody (8E10.1D6) [NBP2-36488] - HeLa cells were treated with valinomycin (1 uM, 24h) prior to being fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% Triton X-100 in PBS for 10 min. Cells were incubated with NBP2-36488 at 50 ug/ml for 1hr at room temperature, washed 3x in PBS and incubated with Alexa-DyLight-488 anti-mouse secondary antibody. PINK1 (Green) was detected at the mitochondria. Tubulin (Red) was detected using an anti-tubulin antibody with an anti-rabbit DyLight 550 secondary antibody. DNA (Blue) was counterstained with DAPI. Note: mitochondria staining might not be easily observed without treatment with valinomycin or CCCP. Image objective 40x.](http://images.novusbio.com/fullsize/PINK1-Antibody-8E10-1D6-Immunocytochemistry-Immunofluorescence-NBP2-36488-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PINK1 Antibody (8E10.1D6) [NBP2-36488] - Whole cell protein from HeLa cells treated with or without valinomycin (1 uM, 24h) as indicated was separated by SDS-PAGE on a 7.5% polyacrylamide gel. Protein was transferred to PVDF membrane and probed with 2 ug/ml anti-PINK1 in 1% BSA and detected with an HRP-conjugated anti-mouse secondary antibody using chemiluminescence. PINK1 is seen to be upregulated with treament and with a molecular weight at approximately 60 kDa.](http://images.novusbio.com/fullsize/PINK1-Antibody-8E10-1D6-Western-Blot-NBP2-36488-img0006.jpg)
![Western Blot: PINK1 Antibody (8E10.1D6) [NBP2-36488] - Analysis of (A) Partial Recombinant Human PINK-1 protein with estimated molecular weight at 13kDa and (B) Human Liver lysate using PINK1 antibody clone 8E10.1D6 at 3ug/ml concentration, molecular weight ~64 kDa.](http://images.novusbio.com/fullsize/PINK1-Antibody-8E10-1D6-Western-Blot-NBP2-36488-img0001.jpg)
![Western Blot: PINK1 Antibody [NB100-493] - Detection of murine PINK1 using NB100-493. Lane 1: molecular weight marker. Lane 2. MES cell Mitochondria (20 ug) with a band at the observed molecular weight of 63 kDa. Lane 3. MES cytosol (20 ug). Lane 4. MES nuclear (20 ug) as negative control.Lane 5. Purified human cytochrome C (0.1 ug) as PINK1 negative control.](http://images.novusbio.com/fullsize/PINK1-Antibody-Western-Blot-NB100-493-img0003.jpg "Western Blot: PINK1 Antibody [NB100-493] - Detection of murine PINK1 using NB100-493. Lane 1: molecular weight marker. Lane 2. MES cell Mitochondria (20 ug) with a band at the observed molecular weight of 63 kDa. Lane 3. MES cytosol (20 ug). Lane 4. MES nuclear (20 ug) as negative control.Lane 5. Purified human cytochrome C (0.1 ug) as PINK1 negative control.")
![Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-4H8-Western-Blot-H00009927-M03-img0009.jpg "Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in PC-12 (Cat # L012V1).")
![Immunohistochemistry-Paraffin: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of monoclonal antibody to MFN2 on formalin-fixed paraffin-embedded human heart. Antibody concentration 5 ug/ml](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-4H8-Immunohistochemistry-Paraffin-H00009927-M03-img0007.jpg)
![Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 over-expressed 293 cell line, cotransfected with MFN2 Validated Chimera RNAi ( Cat # H00009927-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MFN2 monoclonal antibody (M03), clone 4H8 (Cat # H00009927-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-4H8-Western-Blot-H00009927-M03-img0004.jpg)
![Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-4H8-Western-Blot-H00009927-M03-img0008.jpg)
![Western Blot: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Analysis of MFN2 expression in transfected 293T cell line by MFN2 monoclonal antibody (M03), clone 4H8. Lane 1: MFN2 transfected lysatE (86.4 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-4H8-Western-Blot-H00009927-M03-img0010.jpg)
![ELISA: Mitofusin 2 Antibody (4H8) [H00009927-M03] - Detection limit for recombinant GST tagged MFN2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-4H8-ELISA-H00009927-M03-img0011.jpg)
![Western Blot: Mitofilin Antibody [NB100-1919] - Western blot of dog testis. Total protein extract 1:2000 dilution of antibody.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NB100-1919-img0006.jpg "Western Blot: Mitofilin Antibody [NB100-1919] - Western blot of dog testis. Total protein extract 1:2000 dilution of antibody.")
![Immunocytochemistry/Immunofluorescence: Mitofilin Antibody [NB100-1919] - HeLa cells with antibody 1:500 dilution labeling mitochondria.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1919-img0004.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. The representative image shows a specific and expected granular staining of Mitofilin in the keratinocytes as well as the other cells of epidermal layer with no staining in basal cells. [60X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0009.jpg)
![Western Blot: Mitofilin Antibody [NB100-1919] - COA7 pathological variants are import-defective. HEK293 cells that transiently expressed wild type or mutant COA7HIS were solubilized, and the affinity purification of COA7HIS was performed. The samples were analyzed by reducing and non-reducing SDS-PAGE and Western blot. Load: 3%. Eluate: 100%. Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/10.15252/emmm.201809561), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Western-Blot-NB100-1919-img0012.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P analysis of Mitofilin/Mitochondrial inner membrane protein in a transverse section of normal human skeletal muscle using Mitofilin antibody at 1:200 dilution. The myocytes showed an expected and very specific granular staining in the cytoplasm. [10X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0011.jpg)
![Immunohistochemistry-Paraffin: Mitofilin Antibody [NB100-1919] - IHC-P detection of Mitofilin/Mitochondrial inner membrane protein in a section of normal human skin using Mitofilin antibody at 1:200 dilution. Specific cytoplasmic positivity of Mitofilin was observed in the keratinocytes as well as other cells of the epidermal layer except for basal cells and dermal connective tissue. [10X Magnification]](http://images.novusbio.com/fullsize/Mitofilin-Antibody-Immunohistochemistry-Paraffin-NB100-1919-img0010.jpg)
![Western Blot: Mitofusin 1 Antibody [NBP1-51841] - Densitometric analyses of protein expression levels relative to the sedentary group with representative image of western blot are shown. n = 5-6/group. Values are expressed by mean +/- SEM expressed as fold change relative to the sedentary group. * P < 0.05, significantly different from the sedentary group. Black bar: fetal hearts from sedentary dams; gray bar: fetal hearts from exercised dams. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC5350185/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-Western-Blot-NBP1-51841-img0010.jpg "Western Blot: Mitofusin 1 Antibody [NBP1-51841] - Densitometric analyses of protein expression levels relative to the sedentary group with representative image of western blot are shown. n = 5-6/group. Values are expressed by mean +/- SEM expressed as fold change relative to the sedentary group. * P < 0.05, significantly different from the sedentary group. Black bar: fetal hearts from sedentary dams; gray bar: fetal hearts from exercised dams. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC5350185/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody [NBP1-51841] - Mfn-1 expressed in mouse neural stem cells. Image from verified customer review.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51841-img0008.jpg)
![Immunohistochemistry: Mitofusin 1 Antibody [NBP1-51841] - IHC staining of Mfn1 in mouse intestine.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-Immunohistochemistry-NBP1-51841-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody [NBP1-51841] - Mitofusin-1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51841-img0007.jpg)
![Western Blot: Mitofusin 2 Antibody [NBP2-66383] - Total protein from human HeLa and A431, mouse Neuro2A and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-MFN2 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-Western-Blot-NBP2-66383-img0003.jpg "Western Blot: Mitofusin 2 Antibody [NBP2-66383] - Total protein from human HeLa and A431, mouse Neuro2A and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-MFN2 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Mitofusin 2 Antibody [NBP2-66383] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Mitofusin 2 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-66383-img0002.jpg)
![Immunohistochemistry-Paraffin: Mitofusin 2 Antibody [NBP2-66383] - Analysis of FFPE tissue section of mouse heart using Mitofusin 2/MFN2 antibody at 5 ug/mL concentration (1:200 dilution). The primary antibody binding to MFN2 antigen was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This MFN2 antibody generated a diffused to punctate cytoplasmic staining of mitochondria in the cardiac muscles (brown color). The nuclei of the muscle cells and the macrophages are stained blue in this image (40X image of a transverse section of heart tissue).](http://images.novusbio.com/fullsize/Mitofusin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-66383-img0001.jpg)
![Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Effect of AIF overexpression on mitochondrial fission and fusion in the cortex. Immunoblotting of OPA1 and Mitofusin 1 in the mitochondrial fraction from cortical tissue of the CL and IL hemispheres at 24 h after HI in WT and AIF Tg mice. The expression of Mitofusin 1 was significantly decreased at 24 h post-HI both in WT and AIF Tg mice, while the short form (82 kDa) and cleavage band of OPA1 were significantly increased at 24 h post-HI in both the WT and AIF Tg mice. Quantification of OPA1 and Mitofusin 1 did not show any significant differences in the CL and IL hemispheres between WT and AIF Tg mice (n=6/group). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2280-z), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Western-Blot-NBP1-71775-img0014.jpg "Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Effect of AIF overexpression on mitochondrial fission and fusion in the cortex. Immunoblotting of OPA1 and Mitofusin 1 in the mitochondrial fraction from cortical tissue of the CL and IL hemispheres at 24 h after HI in WT and AIF Tg mice. The expression of Mitofusin 1 was significantly decreased at 24 h post-HI both in WT and AIF Tg mice, while the short form (82 kDa) and cleavage band of OPA1 were significantly increased at 24 h post-HI in both the WT and AIF Tg mice. Quantification of OPA1 and Mitofusin 1 did not show any significant differences in the CL and IL hemispheres between WT and AIF Tg mice (n=6/group). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2280-z), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Antibody was tested in 3T3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Immunocytochemistry-Immunofluorescence-NBP1-71775-img0013.jpg)
![Immunohistochemistry-Paraffin: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 in mouse liver.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Immunohistochemistry-Paraffin-NBP1-71775-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 expression in (1) MEF wild-type and (2) MEF Mitofusin-1-null whole cell lysates using NBP1-71775.](http://images.novusbio.com/fullsize/Mitofusin-1-Antibody-11E9-1H12-Western-Blot-NBP1-71775-img0009.jpg)
![Western Blot: PDK4 Antibody [NBP1-07047] - Western Blot on human heart protein lysate.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0002.jpg "Western Blot: PDK4 Antibody [NBP1-07047] - Western Blot on human heart protein lysate.")
![Western Blot: PDK4 Antibody [NBP1-07047] - Mouse liver. NuPAGE 4-12%. This image was submitted via customer review.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0003.jpg)
![Western Blot: PDK4 Antibody [NBP1-07047] - The protein expression levels in QF muscle. Representative Western blot images. n = 14-15 animals/group. * = vs. LFsed (P 0.1). Black bars = sedentary, grey bars = running. Image collected and cropped by CiteAb from the following publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-53), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP1-07047-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0015.jpg "Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: HSP60 Antibody [NBP1-77397] - Analysis of extracts from HeLa and COS cells using NBP1-77397 Hsp60 antibody at 1:1000](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-77397-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77397] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0019.jpg)
![Immunohistochemistry: HSP60 Antibody [NBP1-77397] - Staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunohistochemistry-NBP1-77397-img0006.jpg)
![Flow Cytometry: HSP60 Antibody [NBP1-77397] - Analysis of HSP60 in RM1 cells (murine prostate cancer cell line) using anti-HSP60 antibody. The primary antibody was used at a dilution of 1:100. Image from verified customer review.](http://images.novusbio.com/fullsize/HSP60-Antibody-Flow-Cytometry-NBP1-77397-img0014.jpg)
![Western Blot: HSP60 Antibody [NBP1-77397] - Analysis of HSP60 in: 1) HeLa, 2) HepG2, 3) NIH/3T3, 4) Jurkat, 5) CHO, 6) A431, 7) PC12 and 8) COS7](http://images.novusbio.com/fullsize/HSP60-Antibody-Western-Blot-NBP1-77397-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HSP60 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescence analysis of HeLa cells using Hsp60 antibody at 1:50 (green). Nuclei were counterstained using DAPI (blue).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - Confocal immunofluorescent analysis of HeLa cells using Hsp60 antibody (NBP1-77397, 1:100). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody [NBP1-77397] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody conjugated to Alexa Fluor 488 (NBP1-77397AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77397] - Mouse MS1 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody - BSA Free [NBP1-77397] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with HSP60 Antibody (NBP1-77397) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/HSP60-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-77397-img0018.jpg)
![Simple Western: HSP60 Antibody [NBP1-77397] - Lane view shows a specific band for Hsp60 in 0.05 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-Simple-Western-NBP1-77397-img0013.jpg)