Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - MDA-MB-231 cells were treated with DMSO or Merbarone (100 uM) for 24 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. KAP1 [p Ser824] Antibody (NB100-2350) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Western-Blot-NB100-2350-img0019.jpg "Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - MDA-MB-231 cells were treated with DMSO or Merbarone (100 uM) for 24 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. KAP1 [p Ser824] Antibody (NB100-2350) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody [NB100-2350] - NBF-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with EPE (left) or untreated (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1ug/ml). Detection: Red fluorescent Anti-rabbit IgG-DyLight (R) 594 conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2350-img0015.jpg)
![Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - Human Phospho Kap1 (S824) by Immunohistochemistry. Sample: FFPE serial section of human lung cancer used at a dilution of 1:200 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunohistochemistry-Paraffin-NB100-2350-img0014.jpg)
![Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (15 ug) from NIH 3T3 cellstreated with 100 uM etoposide (+) or mock treated (-).Antibodies: Affinity purified rabbit anti-Phospho KAP-1(S824) antibody used forWB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 30 seconds. Lower panel shows westernblot for total KAP-1 using rabbit anti-KAP-1 recombinantmonoclonal antibody at 1:1000.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Western-Blot-NB100-2350-img0017.jpg)
![Western Blot: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (50 ug) from HEK293Tcells treated with 100 uM etoposide (+) or mock treated(-). Antibodies: Affinity purified rabbit anti-Phospho KAP-1(S824) antibody used forWB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 1 second. Lower panel shows westernblot for total KAP-1 using affinity purified rabbit anti KAP-1 antibody at 0.1 ug/ml.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Western-Blot-NB100-2350-img0018.jpg)
![Immunohistochemistry-Paraffin: KAP1 [p Ser824] Antibody [NB100-2350] - FFPE serial sections of asynchronous HeLa cells treated with EPE (left) anduntreated HeLa cells (right). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunohistochemistry-Paraffin-NB100-2350-img0013.jpg)
![Immunoprecipitation: KAP1 [p Ser824] Antibody [NB100-2350] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) and rabbit anti-KAP-1 recombinant monoclonal antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Phospho KAP-1 (S824), Antibody was used at 0.04 ug/ml. To examine total KAP-1, the blot was stripped and then blotted with this antibody at 1:1000 (lower panel). Detection: Chemiluminescence with an exposure time of 1 second.Previous](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunoprecipitation-NB100-2350-img0016.jpg)
![Simple Western: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from HEK293T cells treated with 100 µM etoposide prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (NBP2-76398) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76398_rabbit-monoclonal-kap1-p-ser824-antibody-bl-246-7b5-30320239244.jpg "Simple Western: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from HEK293T cells treated with 100 µM etoposide prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (NBP2-76398) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.")
![Western Blot: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) by WB of HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Lower panel shows WB for total KAP-1 using rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-NBP2-76398-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP1 (S824) in FFPE breast carcinoma by IHC and in FFPE EPE treated HeLa cells by ICC. Mock phosphatase treated sections (left) and calf intestinal phosphatase-treated sections (right). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunocytochemistry-NBP2-76398-img0004.jpg)
![Immunohistochemistry: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunohistochemistry-NBP2-76398-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phospho KAP-1 (S824) (red) in formaldehyde-fixed asynchronous HeLa cells treated with EPE (right) or untreated (left) by ICC-IF. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398). Secondary: DyLight 594-conjugated goat anti-rabbit IgG. Counterstain: Phalloidin conjugated Alexafluor 488 (green).](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunocytochemistry-Immunofluorescence-NBP2-76398-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Detection of human Phopsho KAP-1 (S824) by WB of immunoprecipitates from HEK293T lysate treated with 100 uM EPE (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (NBP2-76398) and rabbit anti-KAP-1 recombinant monoclonal [BL-248-2G6] (NBP2-76411).](http://images.novusbio.com/fullsize/KAP1-p-Ser284-Antibody-BL-246-7B5-Immunoprecipitation-NBP2-76398-img0005.jpg)
![Flow Cytometry- KAP1 [p Ser824] Antibody (BL-246-7B5) [NBP2-76398] - Antibody: Rabbitanti-phospho KAP-1 recombinant monoclonal[BL-246-7B5] or isotype control (unshaded).Secondary: DyLight® 488-conjugated goat anti-rabbit IgG](http://images.novusbio.com/fullsize/nbp2-76398_rabbit-monoclonal-kap1-p-ser824-antibody-bl-246-7b5-flow-cytometry-30112023101053..jpg)
![Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073] - Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody NBP2-32073 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Western blot for total KAP-1 using a different affinity purified rabbit anti-KAP-1 antibody at 0.4 ug/ml with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Western-Blot-NBP2-32073-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073] - Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 cells treated with etoposide (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho KAP-1 (S824) antibody NBP2-32073 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Western blot for total KAP-1 using a different affinity purified rabbit anti-KAP-1 antibody at 0.4 ug/ml with an exposure time of 3 seconds.")
![<b>Independent Antibodies Validation. </b>Western Blot: KAP1 [p Ser824] Antibody [NBP2-32073] - Detection of mouse Phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from TCMK-1 cells treated with etoposide (+) and/or Lambda PP (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-KAP-1 antibody NBP2-32073 used for IP at 6 ug per reaction. For blotting immunoprecipitated Phospho KAP-1 (S824), NBP2-32073 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-KAP-1 antibody A304-145A.](http://images.novusbio.com/fullsize/KAP1-[p-Ser824]-Antibody-Immunoprecipitation-NBP2-32073-img0002.jpg)
![Western Blot: KAP1 [p Ser 824] Antibody (SR1057) [NBP3-21591] - Western blot analysis using NBP3-21591 in HeLa cell treated with etoposide.](http://images.novusbio.com/fullsize/nbp3-21591_rabbit-kap1-p-ser-824-mab-sr1057-57202317161890.jpg "Western Blot: KAP1 [p Ser 824] Antibody (SR1057) [NBP3-21591] - Western blot analysis using NBP3-21591 in HeLa cell treated with etoposide.")