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![Western Blot: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Western blot analysis of extracts of various cell lines, using IkB-alpha Rabbit mAb (NBP3-16912) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37C for overnight or treated by LPS (1 ug/mL) at 37C for 6 hours.Both NIH/3T3 cells and C6 cells were treated by TNF-alpha (20 ng/mL) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/IkB-alpha-p-Ser36-Antibody-8G1X10-Western-Blot-NBP3-16912-img0004.jpg "Western Blot: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Western blot analysis of extracts of various cell lines, using IkB-alpha Rabbit mAb (NBP3-16912) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37C for overnight or treated by LPS (1 ug/mL) at 37C for 6 hours.Both NIH/3T3 cells and C6 cells were treated by TNF-alpha (20 ng/mL) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry of paraffin-embedded mouse liver using IkB-alpha Rabbit mAb (NBP3-16912) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IkB-alpha-p-Ser36-Antibody-8G1X10-Immunohistochemistry-Paraffin-NBP3-16912-img0003.jpg)
![Immunohistochemistry-Paraffin: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry of paraffin-embedded rat ovary using IkB-alpha Rabbit mAb (NBP3-16912) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IkB-alpha-p-Ser36-Antibody-8G1X10-Immunohistochemistry-Paraffin-NBP3-16912-img0001.jpg)
![Immunohistochemistry-Paraffin: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry of paraffin-embedded human colon using IkB-alpha Rabbit mAb (NBP3-16912) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IkB-alpha-p-Ser36-Antibody-8G1X10-Immunohistochemistry-Paraffin-NBP3-16912-img0002.jpg)
![Western Blot: Histone H2B type 1B [p Ser36] Antibody [NBP3-23378] - Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B type 1B (C-terminus) (lanes 1 & 2), anti-Histone H2B type 1B (Ser-36) (lanes 3 & 4), and anti-Histone H2B type 1B (a.a. 33-47) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23378_rabbit-histone-h2b-type-1b-p-ser36-pab-18920231735528.jpg "Western Blot: Histone H2B type 1B [p Ser36] Antibody [NBP3-23378] - Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B type 1B (C-terminus) (lanes 1 & 2), anti-Histone H2B type 1B (Ser-36) (lanes 3 & 4), and anti-Histone H2B type 1B (a.a. 33-47) (lanes 5 & 6).")
![Western Blot: IkB-alpha [p Ser 36] Antibody (SR1657) [NBP3-21594] - Western blot analysis using NBP3-21594 in HeLa cell lysate treated with Calyculin A.](http://images.novusbio.com/fullsize/nbp3-21594_rabbit-ikb-alpha-p-ser-36-mab-sr1657-57202316582171.jpg "Western Blot: IkB-alpha [p Ser 36] Antibody (SR1657) [NBP3-21594] - Western blot analysis using NBP3-21594 in HeLa cell lysate treated with Calyculin A.")