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![Western Blot: Aldolase C Antibody (4A9) [NBP2-25145] - Human breast cancer MCF7 cells transfected with Aldolase C guide RNA. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-4A9-Western-Blot-NBP2-25145-img0006.jpg "Western Blot: Aldolase C Antibody (4A9) [NBP2-25145] - Human breast cancer MCF7 cells transfected with Aldolase C guide RNA. Western blot image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (4A9) [NBP2-25145] - A. Immunofluorescence of rat cerebellum section stained with mouse mAb to aldolase C, NBP2-25145, dilution 1:1000, in green, and costained with rabbit pAb to GFAP, dilution 1:5000 in red. In both A and B blue is Hoechst staining of nuclear DNA. Aldolase C antibody selectively labels the perikarya and dendrites of Purkinje cells, while GFAP antibody stains processes of Bergman glia and astrocytic cells. B. Immunofluorescence of E20 rat cortical culture stained with NBP2-25145, dilution 1:1,000 in green, and costained with chicken pAb to MAP2, dilution 1:10,000 in red. The aldolase C antibody labels cytosolic protein expressed in glial cells, while MAP2 antibody stains dendrites and perikarya of mature neurons.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-4A9-Immunocytochemistry-Immunofluorescence-NBP2-25145-img0004.jpg)
![Western Blot: Aldolase C Antibody (4A9) [NBP2-25145] - Analysis of tissue and lysates using mouse mAb to aldolase C, NBP2-25145, dilution 1:2000 in green: [1] protein standard (in red), [2] rat brain, [3] mouse brain, [4] cow cerebellum, and [5] pig hippocampus. The single band at about 40 kDa corresponds to the aldolase C protein.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-4A9-Western-Blot-NBP2-25145-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (4A9) [NBP2-25145] - View of mixed neuron/glial cultures stained with NBP2-25145 (green) and the rabbit antibody to RBFOX3/NeuN (NBP1-92716, red). NBP2-25145 reveals strong cytoplasmic staining in astrocytes, while the rabbit RBFOX3/NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and complete absence of astrocytes. Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-4A9-Immunocytochemistry-Immunofluorescence-NBP2-25145-img0003.jpg)
![Immunohistochemistry-Paraffin: Aldolase C Antibody [NBP1-90954] - Analysis in human cerebral cortex and pancreas tissues. Corresponding ALDOC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-Immunohistochemistry-Paraffin-NBP1-90954-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aldolase C Antibody [NBP1-90954] - Analysis in human cerebral cortex and pancreas tissues. Corresponding ALDOC RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Aldolase C Antibody [NBP1-90954] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-Western-Blot-NBP1-90954-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Aldolase C Antibody [NBP1-90954] - Staining of human cell line A-431 shows localization to nucleus, nucleoli fibrillar center & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90954-img0009.jpg)
![Immunohistochemistry-Paraffin: Aldolase C Antibody [NBP1-90954] - Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of glial cells.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-Immunohistochemistry-Paraffin-NBP1-90954-img0018.jpg)
![Western Blot: Aldolase C Antibody [NBP1-90954] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-Western-Blot-NBP1-90954-img0007.jpg)
![Immunohistochemistry-Paraffin: Aldolase C Antibody [NBP1-90954] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-Immunohistochemistry-Paraffin-NBP1-90954-img0016.jpg)
![Immunohistochemistry-Paraffin: Aldolase C Antibody [NBP1-90954] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-Immunohistochemistry-Paraffin-NBP1-90954-img0017.jpg)
![Immunohistochemistry-Paraffin: Aldolase C Antibody [NBP1-90954] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-Immunohistochemistry-Paraffin-NBP1-90954-img0019.jpg)
![Western Blot: Aldolase C Antibody (1A1) [NBP2-25144] - Blots of crude rat brain lysates. The 1A1 clone binds strongly and cleanly to a band at about 40 kDa.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-1A1-Western-Blot-NBP2-25144-img0001.jpg "Western Blot: Aldolase C Antibody (1A1) [NBP2-25144] - Blots of crude rat brain lysates. The 1A1 clone binds strongly and cleanly to a band at about 40 kDa.")
![Immunocytochemistry/Immunofluorescence: Aldolase C Antibody (1A1) [NBP2-25144] - View of mixed neuron/glial cultures stained with NBP2-25144 (green) and the rabbit antibody to RBFOX3/NeuN (NBP1-92716, red). NBP2-25144 reveals strong cytoplasmic staining in astrocytes, while the rabbit RBFOX3/NeuN antibody shows nuclear and distal cytoplasmic staining in neuron cells and complete absence of astrocytes. Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-1A1-Immunocytochemistry-Immunofluorescence-NBP2-25144-img0003.jpg)
![Flow Cytometry: Aldolase C Antibody (1A1) [NBP2-25144] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Aldolase C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-1A1-Flow-Cytometry-NBP2-25144-img0004.jpg)
![Western Blot: Aldolase C Antibody [NBP2-15346] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase C antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-Western-Blot-NBP2-15346-img0006.jpg "Western Blot: Aldolase C Antibody [NBP2-15346] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase C antibody [N1C1] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Frozen: Aldolase C Antibody [NBP2-15346] - Frozen-sectioned adult mouse cerebellum. Green: Aldolase C protein stained by Aldolase C antibody [N1C1] diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-Immunohistochemistry-Frozen-NBP2-15346-img0004.jpg)
![Western Blot: Aldolase C Antibody [NBP2-15346] - Sample (30 ug of whole cell lysate) A: U87-MG B: MCF-7 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-Western-Blot-NBP2-15346-img0001.jpg)
![Western Blot: Aldolase C Antibody [NBP1-91323] - Human Placenta lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Aldolase-C-Antibody-Western-Blot-NBP1-91323-img0002.jpg "Western Blot: Aldolase C Antibody [NBP1-91323] - Human Placenta lysate, concentration 0.2-1 ug/ml.")