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![Western Blot: Podocalyxin Like Antibody [NB110-41503] - Analysis of Podolyxin in 1. Human thymus and 2. Human lymph node.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Western-Blot-NB110-41503-img0001.jpg "Western Blot: Podocalyxin Like Antibody [NB110-41503] - Analysis of Podolyxin in 1. Human thymus and 2. Human lymph node.")
![Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody [NB110-41503] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Podocalyxin Like Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunocytochemistry-Immunofluorescence-NB110-41503-img0007.jpg)
![Western Blot: Podocalyxin Like Antibody [NBP1-83348] - Analysis in control (vector only transfected HEK293T lysate) and PODXL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Western-Blot-NBP1-83348-img0013.jpg "Western Blot: Podocalyxin Like Antibody [NBP1-83348] - Analysis in control (vector only transfected HEK293T lysate) and PODXL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: Podocalyxin Like Antibody [NBP1-83348] - Staining of human cell line U-251 MG shows localization to nucleoli, plasma membrane, microtubule organizing center & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83348-img0012.jpg)
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP1-83348-img0017.jpg)
![Western Blot: Podocalyxin Like Antibody [NBP1-83348] - Analysis in human kidney tissue.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Western-Blot-NBP1-83348-img0007.jpg)
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP1-83348-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Analysis in human kidney and liver tissues. Corresponding Podocalyxin Like RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP1-83348-img0014.jpg)
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human fallopian tube shows strong positivity in cilia in glandular cells.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP1-83348-img0015.jpg)
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP1-83348] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP1-83348-img0016.jpg)
![Western Blot: Podocalyxin Like Antibody [NBP2-13784] - Analysis in control (vector only transfected HEK293T lysate) and PODXL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Western-Blot-NBP2-13784-img0005.jpg "Western Blot: Podocalyxin Like Antibody [NBP2-13784] - Analysis in control (vector only transfected HEK293T lysate) and PODXL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP2-13784] - Staining in human kidney and liver tissues using anti-PODXL antibody. Corresponding PODXL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP2-13784-img0004.jpg)
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP2-13784] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP2-13784-img0002.jpg)
![Immunohistochemistry-Paraffin: Podocalyxin Like Antibody [NBP2-13784] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Podocalyxin-Like-Antibody-Immunohistochemistry-Paraffin-NBP2-13784-img0003.jpg)