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17 Results for "novus"

NB100-1762

LSD1 Antibody (1B2E5)

(12 Publications)

0.1 ml

NB100-1762

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. Strong KDM1A protein expression was confirmed in the medulloblastic tumors from SmoA1-and Ptch+/A-mice relative to KDM1A expression in cerebellar tissue (CB) using western blotting of tissue lysates. I2-actin expression was used as a loading control. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. KDM1A protein expresion was evaluated immunohistochemically in a tissue microarray of 70 medulloblastomas (MB) and 9 tissue samples of normal cerebellum (CB). Micrograph showing KDM1A-positive staining in a representative MB sample, and KDM1A-negative staining in CB, scale bar=100AI1/4m. Image collected and cropped by Citeab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY license.

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.

Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Analysis of LSD1 expression in Hela (1) and Jurkat (2) whole cell lysates.

Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining.

Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - Immunohistochemical analysis of paraffin-embedded Human Kidney Carcinoma tissue, showing nuclear localization using LSD1 antibody with DAB staining.

Simple Western: LSD1 Antibody (1B2E5) [NB100-1762] - Simple Western lane view shows a specific band for LSD1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

<b>Genetic Strategies Validation. </b>Knockdown Validated: LSD1 Antibody (1B2E5) [NB100-1762] - Knockdown of KDM1A protein was confirmed by western blotting of whole-cell lysates from DAOY and ONS-76 cells. beta-actin served as loading control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24252778/) licensed under a CC-BY license.

Plasma membrane associated APE1/Ref-1 is bound to ABCA1 in response to acetylation. Cells transiently expressing wild type APE1/Ref-1-FLAG or mutant APE1/Ref-1(K6/7R)-FLAG were treated with 1 µM TSA for 1 h. (A) Whole cell lysates were immunoprecipitated using the monoclonal anti-ABCA1 antibody, followed by immunoblot with the anti-FLAG antibody. (B) For reverse immunoprecipitation, cell lysates were immunoprecipitated with anti-APE1/Ref-1 antibody followed by immunoblot analysis with the polyclonal anti-ABCA1 antibody. Blots were stripped and re-probed with anti-ABCA1 or FLAG antibodies to ensure equal protein loading and no contamination of cellular proteins. Similar results were observed in replicate experiments. Columns, mean (n = 2-3); bars, SE. *, p < 0.05 indicates a significantly different result from control cells according to unpaired t-tests. (C) The binding between APE1/Ref-1 and ABCA1 in the plasma membrane was visualized using with a Duolink II PLA system with primary polyclonal anti-APE1/Ref-1 and monoclonal anti-ABCA1 antibodies (PLA†). The PLA-specific fluorescence which represents the APE1/Ref-1-ABCA1 signal, and the DAPI nuclear staining are in red and blue, respectively. The experiment was repeated multiple times with similar results; the data shown here are from a representative experiment. Optical slices were examined using a 40× oil immersion objective with a 2× zoom factor. Scale bar, 20 µm (×80). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31261750), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Involvement of N- terminus and C- terminus HIF-2 alpha in IH-induced HIF-2 alpha degradation by calpains. A. Schematic diagram showing the full length, N-terminus ( delta bHLH and delta bHLH+ delta PAS) and C-terminus ( delta UR, delta UR + delta CTAD) deleted constructs. B. Western blot showing HIF-2 alpha protein in PC12 cells transiently transfected with the HIF-2 alpha full length and the two N- and C-terminus truncated constructs and exposed to normoxia (N) or IH. The N-terminus and C-terminus deleted HIF-2 alpha proteins were detected with antibody raised against HIF-2 alpha N-terminus (Acris Antibodies; AP23352PU-N) and C-terminus (Novus Biologicals; NB100-122) respectively. C. PC12 cell lysates expressing the N- and C-terminus deleted protein were incubated for 15 min with purified calpain-1 (3 mg/ml) in presence of 1 mM CaCl2 or 1 mM CaCl2+2 mM EDTA and HIF-2 alpha protein was analyzed by western blot. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24124516), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

PHD3 depletion stabilizes hypoxic p27 expression by increasing p27 half-life. a Cell cycle arrest at G0 and subsequent release shows an increase of p27 expression in siPHD3 exposed cells. b Quantification for p27 expression under PHD3 depletion at indicated time points after cell cycle release in HeLa and 786-O cells. Asterisk indicates significant difference (p < 0,05; n = 3). c Cell cycle arrest at G0 and inhibition of protein synthesis with cycloheximide indicate increased p27 stability in PHD3 depleted HeLa cells. d Quantification of p27 expression using siPHD3 or control at indicated time points. Four independent experiments (± SEM) are shown (p < 0,05; n = 4). e Analysis of p27 stability in 786–0 cells by cycloheximide chase during reoxygenation after 24 h hypoxia demonstrates markedly increased half-life of p27 upon PHD3 depletion Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26223520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Pm

Species Glossary

Reactivity: Hu, Mu, Pm

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB100-56326

MeCP2 Antibody

(1 Publication)

0.1 mg

NB100-56326

Western Blot: MeCP2 Antibody [NB100-56326] - Analysis of MeCP2 in HeLa cell lysate using this antibody.

Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB100-56326] - This antibody was tested in A431 cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.

Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Mouse liver tissue using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in the hepatocytes, kupffer cells, and in the endothelial cells of blood vessels.

Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Tissue section of mouse pancreas using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in majority of the cells from acini, ducts and Islets of Langerhans in the section.

Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Mouse liver tissue using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in the hepatocytes, kupffer cells, and in the endothelial cells of blood vessels.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-297A

Alt Catalog# IMG-297A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB100-56475

LSD1 Antibody

(1 Review)

0.1 mg

NB100-56475

Western Blot: LSD1 Antibody [NB100-56475] - Analysis of LSD1 in the 1) absence and 2) presence of immunizing peptide in human brain lysate using this antibody. 5 ug/ml.

Reactivity:

Hu, Mu, Rt, Ca, Pm, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm, Ze

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5842A

Alt Catalog# IMG-5842A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-56475AF350
Alexa Fluor 405	NB100-56475AF405
Alexa Fluor 488	NB100-56475AF488
Alexa Fluor 532	NB100-56475AF532
Alexa Fluor 594	NB100-56475AF594
Alexa Fluor 647	NB100-56475AF647
Alexa Fluor 700	NB100-56475AF700
Alexa Fluor 750	NB100-56475AF750
Allophycocyanin	NB100-56475APC
Biotin	NB100-56475B
DyLight 350	NB100-56475UV
DyLight 405	NB100-56475V
DyLight 488	NB100-56475G
DyLight 550	NB100-56475R
DyLight 594	NB100-56475DL594
DyLight 650	NB100-56475C
DyLight 680	NB100-56475FR
DyLight 755	NB100-56475IR
FITC	NB100-56475F
HRP	NB100-56475H
Janelia Fluor 549	NB100-56475JF549
Janelia Fluor 646	NB100-56475JF646
PE	NB100-56475PE
PerCP	NB100-56475PCP
mFluor Violet 450 SE	NB100-56475MFV450
mFluor Violet 500 SE	NB100-56475MFV500
mFluor Violet 610 SE	NB100-56475MFV610

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NB600-1101

MeCP2 Antibody

(5 Publications)

50μg

NB600-1101

Western Blot: MeCP2 Antibody [NB600-1101] - Analysis of HeLa cell extract.

Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB600-1101] - Analysis of Methyl CpG Binding Protein 2 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left).

Immunohistochemistry-Paraffin: MeCP2 Antibody [NB600-1101] - Staining in the nucleus of rat brain tissue (right) compared to a negative control without primary antibody (left).

Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB600-1101] - Analysis of Methyl CpG Binding Protein 2 (green) showing staining in the nucleus of C6 cells (right) compared to a negative control without primary antibody (left).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-81801

GPKOW Antibody

(1 Publication)

0.1 ml

NBP1-81801

Western Blot: GPKOW Antibody [NBP1-81801] - Analysis using Anti-GPKOW antibody NBP1-81801 (A) shows similar pattern to independent antibody NBP1-81800 (B).

<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: GPKOW Antibody [NBP1-81801] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81801] - Staining of human pancreas using Anti-GPKOW antibody NBP1-81801.

Western Blot: GPKOW Antibody [NBP1-81801] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81801] - Staining of human fallopian tube shows nuclear positivity in glandular cells.

<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81801] - Staining of human cerebral cortex, colon, kidney and pancreas using Anti-GPKOW antibody NBP1-81801 (A) shows similar protein distribution across tissues to independent antibody NBP1-81800 (B).

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81801] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons and i glial cells.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81801] - Staining of human pancreas shows moderate to strong nuclear positivity in exocrine glandular cells and in islets of Langerhans.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81801] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81801] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules and in cells in glomeruli.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81801] - Staining of human cerebral cortex using Anti-GPKOW antibody NBP1-81801.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81801] - Staining of human colon using Anti-GPKOW antibody NBP1-81801.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81801] - Staining of human kidney using Anti-GPKOW antibody NBP1-81801.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-81800

GPKOW Antibody

(1 Publication)

0.1 ml

NBP1-81800

Knockdown Validated: GPKOW Antibody [NBP1-81800] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GPKOW antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81800] - Staining of human cerebral cortex, colon, kidney and pancreas using Anti-GPKOW antibody NBP1-81800 (A) shows similar protein distribution across tissues to independent antibody NBP1-81801 (B).

<b>Independent Antibodies Validation. </b>Western Blot: GPKOW Antibody [NBP1-81800] - Analysis using Anti-GPKOW antibody NBP1-81800 (A) shows similar pattern to independent antibody NBP1-81801 (B).

Immunocytochemistry/Immunofluorescence: GPKOW Antibody [NBP1-81800] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81800] - Staining of human kidney.

Western Blot: GPKOW Antibody [NBP1-81800] - Analysis in human cell line A-549.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81800] - Staining of human fallopian tube shows nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81800] - Staining of human pancreas.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81800] - Staining of human cerebral cortex.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP1-81800] - Staining of human colon.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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H00004204-M03

MeCP2 Antibody (1B11)

(1 Review)

0.1 mg

H00004204-M03

Western Blot: MeCP2 Antibody (1B11) [H00004204-M03] - Analysis of MECP2 expression in Jurkat cells.

Immunohistochemistry-Paraffin: MeCP2 Antibody (1B11) [H00004204-M03] - Human lymphoma tissue stained with MeCP2 Antibody (1B11). IHC-P image submitted by a verified customer review.

Western Blot: MeCP2 Antibody (1B11) [H00004204-M03] - Analysis of MECP2 expression in HeLa S3 NE.

Immunohistochemistry-Paraffin: MeCP2 Antibody (1B11) [H00004204-M03] - Analysis of monoclonal antibody to MECP2 on FFPE human cerebral cortex. Antibody at 3 ug/mL.

Sandwich ELISA: MeCP2 Antibody (1B11) [H00004204-M03] - Detection limit for recombinant GST tagged MECP2 is approximately 0.1ng/ml as a capture antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00004204-M01

MeCP2 Antibody (4B6)

(2 Publications)

0.1 mg

H00004204-M01

Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - Analysis of MECP2 expression in transfected 293T cell line by MECP2 monoclonal antibody (M01), clone 4B6.Lane 1: MECP2 transfected lysate(52.4 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: MeCP2 Antibody (4B6) [H00004204-M01] - Analysis of monoclonal antibody to MECP2 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.

Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - MECP2 monoclonal antibody (M01), clone 4B6 Analysis of MECP2 expression in MCF-7.

Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - MECP2 monoclonal antibody (M01), clone 4B6. Analysis of MECP2 expression in NIH/3T3.

Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - MECP2 monoclonal antibody (M01), clone 4B6. Analysis of MECP2 expression in rat muscle.

Sandwich ELISA: MeCP2 Antibody (4B6) [H00004204-M01] - Detection limit for recombinant GST tagged MECP2 is approximately 1ng/ml as a capture antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP1-76688

LSD1 Antibody - BSA Free

(1 Publication)

0.1 mg

NBP1-76688

Western Blot: LSD1 Antibody [NBP1-76688] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: LSD1 NBP1-76688 (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry-Paraffin: LSD1 Antibody [NBP1-76688] - Human small intestine tissue with LSD1 antibody at 2 ug/ml.

Western Blot: LSD1 Antibody [NBP1-76688] - P815 cell lysate at (A) 1 and (B) 2 ug/ml.

Immunofluorescence: LSD1 Antibody [NBP1-76688] - Human Small Intestine cells with LSD1 antibody at 20 ug/mL.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-76688AF350
Alexa Fluor 405	NBP1-76688AF405
Alexa Fluor 488	NBP1-76688AF488
Alexa Fluor 532	NBP1-76688AF532
Alexa Fluor 594	NBP1-76688AF594
Alexa Fluor 647	NBP1-76688AF647
Alexa Fluor 700	NBP1-76688AF700
Alexa Fluor 750	NBP1-76688AF750
Biotin	NBP1-76688B
DyLight 350	NBP1-76688UV
DyLight 405	NBP1-76688V
DyLight 488	NBP1-76688G
DyLight 550	NBP1-76688R
DyLight 594	NBP1-76688DL594
DyLight 650	NBP1-76688C
DyLight 680	NBP1-76688FR
DyLight 755	NBP1-76688IR
FITC	NBP1-76688F
HRP	NBP1-76688H
Janelia Fluor 549	NBP1-76688JF549
Janelia Fluor 646	NBP1-76688JF646
PerCP	NBP1-76688PCP
mFluor Violet 450 SE	NBP1-76688MFV450
mFluor Violet 500 SE	NBP1-76688MFV500
mFluor Violet 610 SE	NBP1-76688MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-76688-0.025mg

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NBP1-81027

MeCP2 Antibody

(1 Publication)

0.1 ml

NBP1-81027

Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human cerebellum, lymph node, prostate and testis using Anti-MeCP2 antibody NBP1-81027 (A) shows similar protein distribution across tissues to independent antibody NBP1-81028 (B).

<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP1-81027] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human cerebellum shows strong nuclear positivity in cells in granular layer.

Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB100-536

LSD1 Antibody

(1 Publication)

100 ul

NB100-536

Western Blot: LSD1 Antibody [NB100-536] - Detection of human LSD1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-LSD1 antibody NB100-536 (lot NB100-536-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. LSD1 was also immunoprecipitated by a previous lot (lot NB100-536-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Immunohistochemistry-Paraffin: LSD1 Antibody [NB100-536] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BHC110/LSD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: LSD1 Antibody [NB100-536] - Detection of Human BHC110/KIAA0601 by Western Blot. Samples: Nuclear extract (5 and 20 ug) from HeLa cells. Antibody: NB100-536 used at 0.01 ug/ml. Detection: Chemiluminescence with an exposure time of 15 minutes.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-536AF350
Alexa Fluor 405	NB100-536AF405
Alexa Fluor 488	NB100-536AF488
Alexa Fluor 532	NB100-536AF532
Alexa Fluor 594	NB100-536AF594
Alexa Fluor 647	NB100-536AF647
Alexa Fluor 700	NB100-536AF700
Alexa Fluor 750	NB100-536AF750
Allophycocyanin	NB100-536APC
Biotin	NB100-536B
DyLight 350	NB100-536UV
DyLight 405	NB100-536V
DyLight 488	NB100-536G
DyLight 550	NB100-536R
DyLight 594	NB100-536DL594
DyLight 650	NB100-536C
DyLight 680	NB100-536FR
DyLight 755	NB100-536IR
FITC	NB100-536F
HRP	NB100-536H
Janelia Fluor 549	NB100-536JF549
Janelia Fluor 646	NB100-536JF646
PE	NB100-536PE
PerCP	NB100-536PCP

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NBP2-16714

GPKOW Antibody

0.1 ml

NBP2-16714

Western Blot: GPKOW Antibody [NBP2-16714] - Sample (30 ug of whole cell lysate) A: A549 B: Hela 10% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: GPKOW Antibody [NBP2-16714] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP2-16714] - GPKOW antibody detects GPKOW protein at nucleus in human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded human breast cancer. GPKOW antibody diluted at 1:500.

Immunohistochemistry-Paraffin: GPKOW Antibody [NBP2-16714] - Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using antibody at 1:500 dilution.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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