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![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining in human testis and skeletal muscle tissues using NBP1-88220 antibody. Corresponding CALB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88220-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining in human testis and skeletal muscle tissues using NBP1-88220 antibody. Corresponding CALB2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human cerebellum, skeletal muscle, stomach and testis using Anti-CALB2 antibody NBP1-88220 (A) shows similar protein distribution across tissues to independent antibody NBP1-88221 (B).](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88220-img0020.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Calretinin Antibody [NBP1-88220] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Western-Blot-NBP1-88220-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Calretinin Antibody [NBP1-88220] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88220-img0011.jpg)
![Western Blot: Calretinin Antibody [NBP1-88220] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Western-Blot-NBP1-88220-img0008.jpg)
![Western Blot: Calretinin Antibody [NBP1-88220] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Western-Blot-NBP1-88220-img0010.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human cerebellum shows strong cytoplasmic and nuclear positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88220-img0019.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88220-img0021.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88220-img0022.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88220] - Staining of human testis shows strong cytoplasmic and nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88220-img0023.jpg)
![Western Blot: Calretinin Antibody [NBP1-32244] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE Calretinin antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Western-Blot-NBP1-32244-img0006.jpg "Western Blot: Calretinin Antibody [NBP1-32244] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE Calretinin antibody, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Calretinin Antibody [NBP1-32244] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calretinin protein stained by Calretinin antibody diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32244-img0010.jpg)
![Immunohistochemistry-Frozen: Calretinin Antibody [NBP1-32244] - Frozen Section adult mouse hippocampus. Green: Calretinin protein stained by Calretinin antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Frozen-NBP1-32244-img0017.jpg)
![Western Blot: Calretinin Antibody [NBP1-32244] - A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human CALB2-transfected 293T cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Western-Blot-NBP1-32244-img0008.jpg)
![Western Blot: Calretinin Antibody [NBP1-32244] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calretinin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Western-Blot-NBP1-32244-img0015.jpg)
![Western Blot: Calretinin Antibody [NBP1-32244] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Western-Blot-NBP1-32244-img0005.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-32244] - Mouse fore brain. Calretinin antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-32244-img0007.jpg)
![Immunohistochemistry-Frozen: Calretinin Antibody [NBP1-32244] - Frozen sectioned adult mouse retina. Green: Calretinin protein stained by Calretinin antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Frozen-NBP1-32244-img0013.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-32244] - Mouse retina, using Calretinin antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-32244-img0016.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88221] - Staining in human testis and skeletal muscle tissues using NBP1-88221 antibody. Corresponding CALB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88221-img0038.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88221] - Staining in human testis and skeletal muscle tissues using NBP1-88221 antibody. Corresponding CALB2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88221] - Staining of human cerebellum, skeletal muscle, stomach and testis using Anti-CALB2 antibody NBP1-88221 (A) shows similar protein distribution across tissues to independent antibody NBP1-88220 (B).](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88221-img0034.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Calretinin Antibody [NBP1-88221] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Western-Blot-NBP1-88221-img0032.jpg)
![Immunocytochemistry/Immunofluorescence: Calretinin Antibody [NBP1-88221] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88221-img0017.jpg)
![Western Blot: Calretinin Antibody [NBP1-88221] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Western-Blot-NBP1-88221-img0031.jpg)
![Immunohistochemistry: Calretinin Antibody [NBP1-88221] - Staining of mouse hippocampus shows selective immunoreactivity in the dentate gyrus neurons.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-NBP1-88221-img0018.jpg)
![Immunohistochemistry: Calretinin Antibody [NBP1-88221] - Staining of mouse hypothalamus shows immunoreactivity in neurons of the paraventricular nucleus.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-NBP1-88221-img0019.jpg)
![Immunohistochemistry: Calretinin Antibody [NBP1-88221] - Staining of mouse substantia nigra shows neuronal cell bodies and processes in pars compacta.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-NBP1-88221-img0020.jpg)
![Immunohistochemistry: Calretinin Antibody [NBP1-88221] - Staining of mouse hindbrain shows cytoplasmic immunoreactivity in neurons of the pontine nucleus.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-NBP1-88221-img0021.jpg)
![Immunohistochemistry: Calretinin Antibody [NBP1-88221] - Staining of mouse medulla shows positivity in the gigantocellular reticular nucleus neurons.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-NBP1-88221-img0022.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88221] - Staining of human cerebellum shows strong cytoplasmic and nuclear positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88221-img0033.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88221] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88221-img0035.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88221] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88221-img0036.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-88221] - Staining of human testis shows strong cytoplasmic and nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-88221-img0037.jpg)
![Western Blot: Calretinin Antibody [NBP1-30050] - Rat cerebellum (CB) lysate showing specific immunolabeling of the ~ 29k calretinin protein.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Western-Blot-NBP1-30050-img0001.jpg "Western Blot: Calretinin Antibody [NBP1-30050] - Rat cerebellum (CB) lysate showing specific immunolabeling of the ~ 29k calretinin protein.")
![Immunohistochemistry: Calretinin Antibody [NBP1-30050] - Immunostaining of rat cerebellum showing fluorescence of calretinin containing cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-Immunohistochemistry-NBP1-30050-img0002.jpg)