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![Knockout Validated: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TJP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:200 of Rabbit Anti-Human TJP1 Polyclonal Antibody (Catalog # NBP1-85047) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TJP1 at approximately 260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Knockout-Validated-NBP1-85047-img0019.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TJP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:200 of Rabbit Anti-Human TJP1 Polyclonal Antibody (Catalog # NBP1-85047) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TJP1 at approximately 260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining in human testis and skeletal muscle tissues. Corresponding TJP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0018.jpg)
![Simple Western: Tight Junction Protein 1 Antibody [NBP1-85047] - Simple Western lane view shows a specific band for TJP1 in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Simple-Western-NBP1-85047-img0006.jpg)
![Immunohistochemistry: Tight Junction Protein 1 Antibody [NBP1-85047] - Representative images of DCFA excretion at the biliary poles of corr-FH-iHeps. Left image FH-i, right image Corr-FH-iHeps. All images were taken with 10x objective, z-stacks of xy sections of the cells were acquired with an epifluorescence microscope (Nikon Elipse) and analyzed with ImageJ software. Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-019-1342-6) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-NBP1-85047-img0025.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Effects of dietary Flammulina velutipes stem waste (FVS) inclusion on the relative expression of tight junction proteins in jejunum of growing pigs. a The relative expression of ZO-1 in jejunum of growing pigs fed control and 2.5% or 5% FVS diets. Values are means (3 pigs per treatment) with standard errors represented by vertical bars. *P < 0.05, **P < 0.01. Image collected and cropped by CiteAb from the following publication (//jasbsci.biomedcentral.com/articles/10.1186/s40104-020-00449-y) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85047-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Rat epithelial cells stained positively with Tight Junction Protein 1. Primary antibody at 1:100. Secondary antibody: goat anti-rabbit-Alexa 488 conjugated at 1:500. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0024.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Analysis in control (vector only transfected HEK293T lysate) and TJP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85047-img0020.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85047-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of Pig trabecular meshwork cells. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human cell line U-2 OS shows localization to cytosol & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - The primary mouse lung endothelial cells were fixed, permeabilized and stained with 1:100 diluted anti-ZO-1 ab for overnight. Samples were washed and subsequently incubated with 1:500 diluted Alexa Fuor 546 at room temperature for 1 hour. ICC/iF image submitted by a verified customer reveiw.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Monolayer of Human ARPE-19 cells on a glass substrate. Tight Junction Protein 1 staining in red. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0023.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0014.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0015.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human skeletal muscle shows no membranous positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0016.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human testis shows moderate to strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0017.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining in human cerebral cortex and skeletal muscle tissues using anti-TJP1 antibody. Corresponding TJP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85046-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining in human cerebral cortex and skeletal muscle tissues using anti-TJP1 antibody. Corresponding TJP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046] - Analysis of Tight Junction Protein 1 in RIPA whole cell lysates of mouse primary choroid plexus epithelial cells using anti-Tight Junction Protein 1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-85046-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining of human cell line U-2 OS shows localization to cytosol & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85046-img0007.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85046-img0011.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85046-img0012.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP2-21051-img0009.jpg "Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP2-21051] - Tight Junction Protein 1 Antibody Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: TJP1 protein stained by ZO-1 antibody, Internal diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-21051-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP2-21051-img0005.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP2-21051-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cellular junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-46111_goat-polyclonal-tight-junction-protein-1-antibody-imgenex-img-31199-187202311342910.jpg "Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cellular junction staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-46111] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-46111_goat-polyclonal-tight-junction-protein-1-antibody-imgenex-img-31199-187202311342922.jpg)
![Western Blot: Tight Junction Protein 1 Antibody [NBP1-91621] - Tight Junction Protein 1 Antibody Rat Lung Lysate 1ug/ml Gel Concentration 6-18%](http://images.novusbio.com/fullsize/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP1-91621-img0002.jpg "Western Blot: Tight Junction Protein 1 Antibody [NBP1-91621] - Tight Junction Protein 1 Antibody Rat Lung Lysate 1ug/ml Gel Concentration 6-18%")