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AF1047

p21/CIP1/CDKN1A Antibody [Unconjugated]

(8 Publications)

100 μg

AF1047

Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p21/CIP1/CDKN1A at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

p21/CIP1/CDKN1A was detected in immersion fixed MCF-7 human breast cancer cell line treated with (left panel) or without (right panel) camptothecin using Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

p21/CIP1/CDKN1A was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 1.7 µg/mL Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MCF‑7 human breast cancer cell line was unstimulated (light orange filled histogram) or treated with 1 μM camphtothecin for 16 hours, then stained with Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1047, dark orange filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 16 hours, loaded at 0.2 mg/mL. A specific band was detected for p21/CIP1/CDKN1A at approximately 30 kDa (as indicated) using 5 µg/mL of Goat Anti-Human p21/CIP1/CDKN1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1047) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF1047

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1047-SP

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MAB1047

p21/CIP1/CDKN1A Antibody (195720) [Unconjugated]

(3 Publications)

200 μg

MAB1047

Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 16 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human p21/CIP1/CDKN1A Monoclonal Antibody (Catalog # MAB1047), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p21/CIP1/CDKN1A at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

p21/CIP1/CDKN1A was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human p21/ CIP1/CDKN1A Monoclonal Antibody (Catalog # MAB1047) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MCF-7 human breast cancer cell line either treated with 1 µM camptothecin (CPT) for 16 hours (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human p21/CIP1/CDKN1A Monoclonal Antibody (Catalog # MAB1047), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC1047U-100UG
Alexa Fluor 350	FAB1047U
Alexa Fluor 405	FAB1047V
Alexa Fluor 405	IC1047V-100UG
Alexa Fluor 488	FAB1047G
Alexa Fluor 488	IC1047G-100UG
Alexa Fluor 532	FAB1047X
Alexa Fluor 594	IC1047T-100UG
Alexa Fluor 594	FAB1047T
Alexa Fluor 647	FAB1047R
Alexa Fluor 647	IC1047R-100UG
Alexa Fluor 700	FAB1047N
Alexa Fluor 700	IC1047N-100UG
Alexa Fluor 750	IC1047S-100UG
Alexa Fluor 750	FAB1047S
Allophycocyanin	FAB1047A
Biotin	FAB1047B
DyLight 350	FAB1047D
DyLight 405	FAB1047E
DyLight 488	FAB1047K
DyLight 550	FAB1047L
DyLight 594	FAB1047M
DyLight 650	FAB1047W
DyLight 680	FAB1047Y
DyLight 755	FAB1047Z
FITC	FAB1047F
HRP	FAB1047H
Janelia Fluor 549	FAB1047I
Janelia Fluor 646	FAB1047J
PE	FAB1047P
PerCP	FAB1047C

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1047-SP
Carrier Free	IC1047V-100UG
Carrier Free	IC1047U-100UG
Carrier Free	IC1047T-100UG
Carrier Free	IC1047S-100UG
Carrier Free	IC1047R-100UG
Carrier Free	IC1047N-100UG
Carrier Free	IC1047G-100UG

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NBP3-21623

p21/CIP1/CDKN1A Antibody (SR1331)

100 ul

NBP3-21623

Western Blot: p21/CIP1/CDKN1A Antibody (SR1331) [NBP3-21623] - Western blot analysis using NBP3-21623 in (1) MCF-7 cell lysate; (2) LnCaP cell lysate.

Immunohistochemistry: p21/CIP1/CDKN1A Antibody (SR1331) [NBP3-21623] - Immunohistochemical analysis of paraffin-embedded human colon, using NBP3-21623.

Immunocytochemistry/Immunofluorescence: p21/CIP1/CDKN1A Antibody (SR1331) [NBP3-21623] - Immunofluorescent analysis of MCF7 cells, using NBP3-21623.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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