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NB300-144

Laminin Antibody

(9 Reviews)

(122 Publications)

0.1 ml

NB300-144

Immunohistological analysis of brain stem section stained with rabbit polyclonal Laminin Antibody [NB300-144], dilution 1:1,000 in red, and costained with chicken pAb to Myelin Basic Protein (MBP), dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with the above antibodies. The laminin antibody is an excellent marker of basement membranes surrounding blood vessels, while the MBP antibody stains the myelin sheathes around axons.

MxIF analysis of mouse submandibular salivary gland morphogenesis. MxIF of a developmental TMA including embryonic stages (E14, E16, E18) and postnatal stages (P5 and P20) was performed using sequential application of directly conjugated antibodies to detect multiple markers of tissue structures and cell types on the same tissue sections. Tissue compartments. The epithelium, mesenchyme, neurons, and basement membranes was detected using antibodies directed towards E-cadherin (ECAD, red), platelet-derived growth factor (PDGFR, green), beta III tubulin (bIII, magenta), and Laminin Antibody [NB300-144] (LMN, cyan), respectively. Image collected and cropped by CiteAb from the following publication (//bio.biologists.org/cgi/doi/10.1242/bio.20134309), licensed under a CC-BY license.

Staining of mouse section of cortex stained with Laminin Antibody [NB300-144] (red). Blue is DAPI staining of DNA. This antibody reveals strong staining in the basement membranes of blood vessels.

Staining of rat spinal cord and dorsal root paraformaldehyde/paraffin-embedded tissue using Laminin Antibody [NB300-144]. Pepsin antigen retrieval was performed on this tissue sample.

Analysis of rat heart cells lysates (lane 1) and 0.2 ug of purified laminin protein from mouse EHS sarcoma (lane 2) Laminin Antibody [NB300-144] recognizes 3 laminin isotypes: alpha 1 (440 kDa), beta 1 (220 kDa) and gamma 1 (220 kDa). Also recognized is a laminin binding protein at 120 kDa in both rat heart lysates and purified laminin protein. Since this protein always coexpresses with laminin this crossreactivity is irrelevant. Theoretical molecular weight of LAMA1 is 337 kDa.

IF Confocal analysis of HeLa cells using Laminin Antibody [NB300-144] (1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).

Western blot analysis of the effects of IP6, Ins, IP6 + Ins and normal saline on the levels of collagen IV, Laminin and Fibronectin, with Laminin Antibody [NB300-144]. IP6 or Ins treatment decreased the protein expression of LN and FN, and the combined IP6 + Ins treatment resulted in significantly greater effects compared with treatment with either compound alone. The Western blot membranes were stripped and reprobed for beta-actin as an internal control to confirm equal loading. (A) representative blots from one of three separate experiments; (B) relative band intensities based on densitometry. The results are expressed as the mean +/- standard deviation from three independent experiments. * p < 0.05 compared to the IP6 + Ins group; #p < 0.05 compared to the normal saline group. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/8/5/286), licensed under a CC-BY license.

Immunofluorescence staining for extracellular matrix proteins in day 1 and day 7 HWJSC spheroids. HWJSC spheroids were cultured for either 1 day or 7 days (with 6 days of osteogenic differentiation), fixed, and stained for extracellular matrix protein: laminin (C) and counter-staining with Hoechst. Scale bars represent 100 um. Image collected and cropped by CiteAb from the following publication (journals.plos.org/plosone/article?id=10.1371/journal.pone.0184155), licensed under a CC-BY license. Using the Alexa Fluor 488 format of this antibody.

Laminin staining on an E12.5 mouse Right Ventricle. IHC-Fr image submitted by a verified customer review.

A surface stain was performed on HeLa cells with the Alexa Fluor 647 conjugate of Laminin Antibody [NB300-144AF647] (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Analysis of Laminin-1 expression in mouse EHS tumor crude extracts (left) and Laminin-2 expression in rat heart crude extracts (right), using Laminin Antibody [NB300-144]. The Laminin polyclonal antibody was used at 1 ug/mL. Theoretical Molecular Weight of NB300-144 is 337 kDa.

VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) using Laminin Antibody [NB300-144] shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (//asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY license.

SOX17 regulates adult muscle regeneration after injury in Pax7CreERT2/+;Sox17fl/fl mutant mice. Representative images of cryosections from regenerating adult TA muscles d28 after injury, showing immunofluorescence for PAX7+ (quiescent, arrows) cells. Scale bar, 25 Aum. Image collected and cropped by Citeab from the following publication (SOXF factors regulate murine satellite cell self-renewal and function through inhibition ofI2-catenin activity. Elife (2018)) licensed under a CC-BY license.

VSOP observed in perivascular-restricted spinal cord lesions with intact BBB (B) Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23452162), licensed under a CC-BY licence.

Reactivity:

Hu, Mu, Rt, ChHa, In, Ma, Rb, Sh

Species Glossary

Reactivity: Hu, Mu, Rt, ChHa, In, Ma, Rb, Sh

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-144AF350
Alexa Fluor 405	NB300-144AF405
Alexa Fluor 488	NB300-144AF488
Alexa Fluor 532	NB300-144AF532
Alexa Fluor 594	NB300-144AF594
Alexa Fluor 647	NB300-144AF647
Alexa Fluor 700	NB300-144AF700
Alexa Fluor 750	NB300-144AF750
Allophycocyanin	NB300-144APC
Allophycocyanin/Cy7	NB300-144APCCY7
Biotin	NB300-144B
DyLight 350	NB300-144UV
DyLight 405	NB300-144V
DyLight 488	NB300-144G
DyLight 550	NB300-144R
DyLight 594	NB300-144DL594
DyLight 650	NB300-144C
DyLight 680	NB300-144FR
DyLight 755	NB300-144IR
FITC	NB300-144F
HRP	NB300-144H
Janelia Fluor 549	NB300-144JF549
Janelia Fluor 646	NB300-144JF646
PE	NB300-144PE
PE/Atto594	NB300-144PEATT594
PE/Cy5.5	NB300-144PECY55
PE/Cy7	NB300-144PECY7
PerCP	NB300-144PCP
mFluor Violet 450 SE	NB300-144MFV450
mFluor Violet 500 SE	NB300-144MFV500
mFluor Violet 610 SE	NB300-144MFV610

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NB100-1770

GFP Antibody

(7 Reviews)

(122 Publications)

0.1 mg

NB100-1770

Analysis using the Biotin conjugate of NB100-1770. Staining of transgenic mouse pancreas, expressing GFP in beta cells. Image courtesy of product review by Dr. Yves Heremans of Vrije Universiteit Brussel.

Analysis of Biotin conjugate of NB100-1770. Tissue: Drosophila melanogaster late stage embryonic central nervous system. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Anti-GFP antibody at a 1:1000 for 1 h at RT.

Analysis using the FITC conjugate of NB100-1770. Lane 1: GFP (50 ug). Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1000 for 60 min at RT. Block for 30 minutes.

HEK293 cells stable transfected with integrin alpha8-GFP, were immunostained for GFP (green) and counterstained with phalloidin (red) and DAPI (BLUE). ICC/IF image submitted by a verified customer review.

Analysis using the Alkaline Phosphatase conjugate of NB100-1770. Lane 1: GFP (50 ng). Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Predicted/Observed size: 28 kDa for GFP.

9 Poly-GA co-immuno - precipitation with pATM. Aggregated poly-GA selectively co-immunoprecipitates with pATM. Image collected and cropped by CiteAb from the following publication (www.link.springer.com/article/10.1007%2Fs00401-019-02082-0) licensed under a CC-BY license.

E5.5 Hex-GFP transgenic mouse embryo tissue. Primary antibody: Goat anti-GFP at 1:500 dilution. Secondary antibody: Fluorochrome conjugated Anti-goat IgG antibody at 1:10,000 for 45 min at RT. Staining: GFP as green fluorescent signal with DAPI blue counterstain.

IF analysis of GFP in mouse brain. Image courtesy of product review by Tatyana Pivneva.

Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS o/n, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Primary antibody: Goat anti-GFP was used at 1:500 dilution in free floating immunohistochemistry to detect GFP. Secondary antibody: Fluorochrome conjugated Anti-goat IgG antibody at 1:500 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.

Analysis of DyLight 488 conjugate of NB100-1770. Human breast carcinoma tissue. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Anti-Histone and Anti-Tubulin antibody at 10 ug/mL for 1 h at RT. Secondary antibody: DyLight 488.

GFP-positive transplanted NPCs have morphological features of hippocampal pyramidal neurons at day 90 after grafting. IHC image submitted by a verified customer review.

Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. Primary antibody: GFP antibody at 1 ug/ml for 1 h at room temperature. Secondary antibody: IRDye(R) 800 conjugated Donkey-a-Goat IgG [H&L] MX7 () secondary antibody at 1:2,500 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa, 33 kDa for GFP. Other band(s): none.

Western Blot of GFP Antibody. Lane 1: Opal Prestained Molecular Weight Marker

Multi-lysate Western Blot of GFP Antibody. Marker: Opal Pre-stained ladder

hnRNPA3 knockout enhances DNA damage in DPR expressing HeLa cells. a Immunocytochemical detection of gammaH2AX foci in GFP-tagged DPR-expressing HeLa WT and A3KO HeLa cells. b Fold change of gammaH2AX foci in HeLa WT and A3KO cells expressing the indicated DPRs relative to HeLa WT cells with GFP expression. N = 47-127 cells from 2 biological replicates. c Immunocytochemical detection of pATM foci in GFP-tagged DPR-expressing HeLa WT and A3KO HeLa cells. d Fold change of pATM foci in HeLa WT and A3KO cells expressing the indicated DPRs relative to HeLa WT cells with GFP expression. N = 71-263 cells from 2 biological replicates. GFP EGFP transfected, GA EGFP-tagged poly-GA 175 repeats transfected, GR EGFP-tagged poly-GR 177 repeats transfected, PR: EGFP-tagged poly-PR 176 repeats transfected. All graphs are shown as mean +/- SEM. *p < 0.05, **p < 0.01; one-way ANOVA and Tukey's post-hoc test. Scale bar 10 um Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31642962), licensed under a CC-BY licence.

HIF1 alpha and PFKFB3 are protective against hIAPP mediated beta -cell death. a Representative immunoblotting (left) and quantification (right) of the whole cell extract (WCE) from asynchronous INS 832/13 cells treated with either HIF1 alpha inhibitor KC7F2 (10 μM) or PFKFB3 siRNA (75 nM) for 56 h versus control. Cells were transduced with hIAPP adenovirus (75 MOI) or LacZ adenovirus as control (75 MOI) for last 32 h of culture. DMSO or CTRL siRNA, respectively were added to LacZ or hIAPP to equal DMSO or PFKFB3 siRNA used in experimental groups. GAPDH was used as a loading control. Data are presented as mean ± SEM, n = 4, *p < 0.05, **p < 0.01. b Quantification of DNA content distribution after FACS analysis of INS 832/13 cells treated as described in (a). Data are the mean ± SEM, n = 4, *p < 0.05, **p < 0.01. c The frequency of TUNEL positive INS 832/13 cells treated as described in (a). Data are the mean ± SEM, n = 4 in (a) and (b), and n = 3 independent experiments in (c). Statistical significance was analyzed by one-way ANOVA test with Tukey’s post-test (*p < 0.05, **p < 0.01). See Supplementary Fig. 10 for additional supportive data Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31213603), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Key molecules of specific signaling pathways are assayed by immunohistochemistry in the colorectum of mice. Immunohistochemistry (200× magnification) of (A) S100a9, (B) beta -catenin, (C) c-Myc, (D) p-Akt, (E) p-Smad2, and (F) Cxcl5 in normal control, IgG Ab, and anti-S100a9 Ab-treated colorectal tissues of the colitis-associated cancer mouse (n = 4). Scale bar, 50 µm. Staining scores were determined by semi-quantitative optical analysis. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29326691), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

All-NA

Species Glossary

Reactivity: All-NA

Applications:

, , , , , , , , , ,

Applications: , , , , , , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alkaline Phosphatase	NB600-1502
Biotin	NB100-1678
DyLight 488	NBP1-69969
FITC	NB100-1771
FITC	NB100-1771SS
HRP	NB600-313
Rhodamine	NBP1-97327-500ug
Texas Red	NB120-6660

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NB100-74555

Tryptophan hydroxylase 2 Antibody - BSA Free

(3 Reviews)

(53 Publications)

100 ul

NB100-74555

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-74555] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using Tryptophan hydroxylase 2 antibody at 1:25 dilution. The staining was developed using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated staining of the neurons.

Western Blot: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Total protein from mouse brain, stomach and human brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Tryptophan Hydroxylase 2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - The 5-HT and Tryptophan hydroxylase 2 in the raphe nuclei of the brain. The 5-HT- and Tryptophan hydroxylase 2-positive double stained cells in the dorsal raphe nuclei. Representative photomicrographs were taken at magnifications of 100x. Quantification of 5-HT and Tryptophan hydroxylase 2. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2218-273X/10/1/71), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis in mouse brain cells. Image courtesy of product review by Assaf Vestin.

Immunohistochemistry Free-Floating: Tryptophan hydroxylase 2 Antibody [NB100-74555] - PFA-perfused mouse midbrain serotoninergic neurons. AlexaFluor488 used as secondary antibody. Neurons pseudocolored in yellow hot LUT. Image from verified customer review.

Immunoprecipitation: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis of raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody. PA1-778 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT. Immunoprecipitated proteins were assessed by Western blotting (WB), using either mouse anti-TPH2 (Ms TPH2, Lane 2) or sheep anti-TPH2 antibodies (Sh TPH2, Lane 3). As a positive control for the Western blot, non-immunoprecipitated brain lysates were probed with the mouse anti-TPH2 antibody (Lane 1). As a specificity control, a blot containing immunoprecipitated proteins was probed with an antibody specific for Tyrosine Hydroxylase (TH, Lane 4). Data courtesy of the Innovators Program.

The 5-HT and TPH2 in the raphe nuclei of the brain. The 5-HT- and TPH2-positive double stained cells in the dorsal raphe nuclei (A). Representative photomicrographs were taken at magnifications of 100×. Quantification of 5-HT and TPH2 (B). Data are expressed as the means ± SD (n = 3). *, p < 0.05; **, p < 0.01 compared with the vehicle group. 5-HT; 5-hydroxytryptamine, TPH2; tryptophan hydroxylase, DAPI; 4′,6-diamidine-2-phenylindole dihydrochloride.

Reactivity:

Hu, Mu, Rt, Pm, Rb

Species Glossary

Reactivity: Hu, Mu, Rt, Pm, Rb

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-74555AF350
Alexa Fluor 405	NB100-74555AF405
Alexa Fluor 488	NB100-74555AF488
Alexa Fluor 532	NB100-74555AF532
Alexa Fluor 594	NB100-74555AF594
Alexa Fluor 647	NB100-74555AF647
Alexa Fluor 700	NB100-74555AF700
Alexa Fluor 750	NB100-74555AF750
Allophycocyanin	NB100-74555APC
Biotin	NB100-74555B
DyLight 350	NB100-74555UV
DyLight 405	NB100-74555V
DyLight 488	NB100-74555G
DyLight 550	NB100-74555R
DyLight 594	NB100-74555DL594
DyLight 650	NB100-74555C
DyLight 680	NB100-74555FR
DyLight 755	NB100-74555IR
FITC	NB100-74555F
HRP	NB100-74555H
Janelia Fluor 549	NB100-74555JF549
Janelia Fluor 646	NB100-74555JF646
PE	NB100-74555PE
PerCP	NB100-74555PCP
mFluor Violet 450 SE	NB100-74555MFV450
mFluor Violet 500 SE	NB100-74555MFV500
mFluor Violet 610 SE	NB100-74555MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-74555-0.025ml

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NBP1-05198

GFAP Antibody

(1 Review)

(40 Publications)

0.1 ml

NBP1-05198

Immunohistochemistry Free-Floating: GFAP Antibody [NBP1-05198] - Staining of a section of mouse hippocampus stained with chicken pAb to GFAP, NBP1-05198, dilution 1:5000 in green and costained with rabbit pAb to FOX3/NeuN, dilution 1:5000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, mouse brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with the above antibodies. The GFAP antibody stains a network of astroglial cells while the Fox3/NeuN antibody stains the nuclei and proximal perikarya of neurons.

Western Blot: GFAP Antibody [NBP1-05198] - Analysis of whole brain lysates using chicken pAb to GFAP, NBP1-05198, dilution 1:5000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain. The strong band at about 50 kDa corresponds to the GFAP protein. Smaller proteolytic fragments and alternate transcripts of GFAP may also be detected on such blots.

Reactivity:

Hu, Mu, Rt, Po, Bv, Eq

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Eq

Applications:

, , ,

Applications: , , ,

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP1-05197

GFAP Antibody (5C10)

(1 Review)

(25 Publications)

0.1 ml

NBP1-05197

Immunohistochemistry Free-Floating: GFAP Antibody (5C10) [NBP1-05197] - Analysis of rat cerebellum section stained with mouse GFAP mAb, dilution 1:1,000 (Green), costained with rabbit neurofilament NF-L pAb, dilution 1:2,000 (Red). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with antibodies. The GFAP antibody stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes.

Simple Western: GFAP Antibody (5C10) [NBP1-05197] - Simple Western lane view shows a specific band for GFAP in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry: GFAP Antibody (5C10) [NBP1-05197] - The immunofluorescence of TLR7 recognized by Alexa 488, green (NBP2-24906). GFAP recognized by Alexa 594, red. NEUN recognized by Alexa 633, (blue) and merged image in the hippocampal region. NeuN and GFAP were applied to show the distribution of TLR7 within neuronal and supportive tissue populations. Scale bar 80 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0222818) licensed under a CC-BY license.

Immunohistochemistry: GFAP Antibody (5C10) [NBP1-05197] - Analysis of rat cerebellum section stained with mouse mAb to GFAP, NBP1-05197, dilution 1:1,000, in green, costained with rabbit pAb to neurofilament NF-L, dilution 1:2,000, in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP1-05197 antibody stains a network of astroglial cells, while the NF-L antibody labels neuronal cells and their processes.

Western Blot: GFAP Antibody (5C10) [NBP1-05197] - Analysis of whole tissue lysates using mouse mAb to GFAP, NBP1-05197, dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 50kDa corresponds to the GFAP protein.

Reactivity:

Hu, Mu, Rt, Po, Bv, Eq

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Eq

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-05197AF350
Alexa Fluor 405	NBP1-05197AF405
Alexa Fluor 488	NBP1-05197AF488
Alexa Fluor 532	NBP1-05197AF532
Alexa Fluor 594	NBP1-05197AF594
Alexa Fluor 647	NBP1-05197AF647
Alexa Fluor 700	NBP1-05197AF700
Alexa Fluor 750	NBP1-05197AF750
Biotin	NBP1-05197B
DyLight 350	NBP1-05197UV
DyLight 405	NBP1-05197V
DyLight 488	NBP1-05197G
DyLight 550	NBP1-05197R
DyLight 594	NBP1-05197DL594
DyLight 650	NBP1-05197C
DyLight 680	NBP1-05197FR
DyLight 755	NBP1-05197IR
FITC	NBP1-05197F
HRP	NBP1-05197H
Janelia Fluor 549	NBP1-05197JF549
Janelia Fluor 646	NBP1-05197JF646
mFluor Violet 450 SE	NBP1-05197MFV450
mFluor Violet 500 SE	NBP1-05197MFV500
mFluor Violet 610 SE	NBP1-05197MFV610

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NB110-60017

Dopamine D1R/DRD1 Antibody (SG2-D1a)

(3 Reviews)

(18 Publications)

0.1 ml

NB110-60017

Western Blot: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of lysates from Sf9 cells which were transfected with rat DR-D1b (Lane 1) or DR-D1a (Lane 2) using DRD1 antibody (clone SG2-D1a). This clone detected D1a receptor only and was not cross-reactive against DR-D1b (Note: these results are similiar to those shown in J. Neuroimmunol. 101:170-187 publication.)

Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Representative images of immunofluorescence for c-fos and dopamine receptor D1 in the NAc. Image collected and cropped by CiteAb from the following publication (nature.com/articles/ncomms11829), licensed under a CC-BY license.

Immunohistochemistry: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine Receptor D1 in rat retinal whole mounts and sections. Image from verified customer review.

Immunohistochemistry Free-Floating: Dopamine D1R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Dopamine D1 R/DRD1 Antibody (SG2-D1a) [NB110-60017] - Analysis of Dopamine D1 R in free floating rat brain slices using Dopamine D1 R antibody. Image from verified customer review.

NAc D1 and D2 neuronal activation predicts performance in motivation-related tasks. Representative images of immunofluorescence for c-fos and dopamine receptor D1 (f) or c-fos and dopamine receptor D2 (i) in the NAc of animals that performed PR task; Scale bar: 100 um; inset: 20 um. Insets represent double positive cells. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms11829), licensed under a CC-BY licence.

Hypoxia and HIF-1 alpha increase GH synthesis.a Effect of hypoxia (1% O2 for 18 h) on GH secretion on nine human acromegalic tumors in primary cell culture. For all cell culture experiments, each GH RIA value was divided to respective cell viability count as determined by WST-1 at OD450nm. Every condition was in quadruplicates and data are means ± SEM. *P < 0.05 to normoxia (NX) (Student’s t test). b Effect of 18 h hypoxia on Gh promoter activity in GH3 cells transfected with the rat Gh-luc plasmid (pA3GHluc). Luc/ beta Gal: luciferase to beta -galactosidase ratio. Data are means ± SEM from three experiments and are presented as percentage of each normoxia (NX). *P < 0.05 to each normoxia (Student’s t test). RLA, relative luciferase activity. c Effect of hypoxia on endogenous rat Gh transcription as determined by real-time RT-PCR. Data are Gh/TfIIb from three independent experiments and presented as fold increase to each normoxia. *P < 0.05 (Student’s t test). d Effect of hypoxia on GH secretion. In b–d transfection with 100 nM HIF-1 alpha siRNA for 48 h abolished the effect of hypoxia. The immunoblot shows as control the HIF-1 alpha siRNA knockdown efficacy in hypoxic cells. Data are means ± SEM from three experiments each done in triplicates. *P < 0.05 to each normoxia (Student’s t test). Effect of HIF-1 alpha overexpression on (e) rat Gh promoter activity, (f) endogenous rat Gh transcription, and (g) secretion. Data are presented as fold increase versus mock control (pCMV empty vector). *P < 0.05 to mock (Student’s t test). h Effect of the HIF-1 alpha mutant HIF-1 alpha R30A on rat Gh promoter activity. Data are means ± SEM from three experiments and are presented as percentage of mock transfected control. Inset shows immunoblot for HIF-1 alpha in the overexpressing cells. i Chromatin immunoprecipitation showing absence of HIF-1 alpha binding to the endogenous rat Gh promoter in GH3 cells under normoxia or hypoxia, in contrast to the positive control Pit-1 (shown in hypoxic cells). Rabbit IgG was used as a control. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32111982), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Mu, Rt, Bv(-), Ca(-), Hu(-), Po(-), Rb(-), Sh(-)

Species Glossary

Reactivity: Mu, Rt, Bv(-), Ca(-), Hu(-), Po(-), Rb(-), Sh(-)

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-60017AF350
Alexa Fluor 405	NB110-60017AF405
Alexa Fluor 488	NB110-60017AF488
Alexa Fluor 532	NB110-60017AF532
Alexa Fluor 594	NB110-60017AF594
Alexa Fluor 647	NB110-60017AF647
Alexa Fluor 700	NB110-60017AF700
Alexa Fluor 750	NB110-60017AF750
Biotin	NB110-60017B
DyLight 350	NB110-60017UV
DyLight 405	NB110-60017V
DyLight 488	NB110-60017G
DyLight 550	NB110-60017R
DyLight 594	NB110-60017DL594
DyLight 650	NB110-60017C
DyLight 680	NB110-60017FR
DyLight 755	NB110-60017IR
FITC	NB110-60017F
HRP	NB110-60017H
Janelia Fluor 549	NB110-60017JF549
Janelia Fluor 646	NB110-60017JF646
mFluor Violet 450 SE	NB110-60017MFV450
mFluor Violet 500 SE	NB110-60017MFV500
mFluor Violet 610 SE	NB110-60017MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-80700

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NBP2-50028

Calbindin D-28K Antibody

(1 Review)

(11 Publications)

0.1 ml

NBP2-50028

Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, NBP2-50028, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] pig hippocampus, [4] cow cerebellum, [5] protein standard (red). Next lanes are full length recombinant human proteins, [6] Parvalbumin, [7] Calretinin, [8] Calbindin. Bands at 25kDa in tissue lysates and ~30 kDa in protein solutions correspond to calbindin, the recombinant form being slightly larger due to the presence of a His tag and other vector derived sequence. NBP2-50028 antibody specifically recognizes calbindin protein and does not react with the closely related proteins parvalbumin and calretinin.

Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Cultured mouse embryonic kidney stained with Calbindin antibody (1:200) and DAPI. Image from verified customer review.

Immunohistochemistry: Calbindin D-28K Antibody [NBP2-50028] - Representative images of horizontal cells (Calbindin D-28K, cyan) in wild type control and C1q-/- mice at P8, P13, P17, and P23. Horizontal cells in C1q-/- mice fail to remain confined to the OPL, showing an increased number of ectopic neurites (arrowheads) that peak at P13 but remain present as development proceeds. These neurites extend into the outer nuclear layer and grow longer over time. Scale bars = 25 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33177995/) licensed under a CC-BY license.

Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of rat brain lysate (left), 0.5 ug of His-tagged recombinant proteins (right) were probed with NBP2-50028 at 1:5,000. Lane 1: Parvalbumin, Lane 2: Calretinin, Lane 3: Calbindin.

Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Adult rat brain cortex (Left) and striatum (Right) sections (45 uM; fixed by transcardial perfusion with 4% paraformaldehyde) were stained with NBP2-50028 (1:1,000, red), and monoclonal mouse anti-RBFOX3/NeuN (NBP1-92693; green). Calbindin labels a subset of sparsely-distributed interneurons (calbindin-positive interneurons) in the cortex (Left), and more densely-distributed neurons in the striatum (Right). Since neurons also express RBFOX3/NeuN, calbindin-positive cells appear to be gold to yellow. Insets are high magnification images of boxed area of each image. Blue is Dapi nucleus staining.

Immunohistochemistry Free-Floating: Calbindin D-28K Antibody [NBP2-50028] - Analysis of rat cerebellum section stained with chicken Calbindin D-28K pAb, dilution 1:2,000 (Green), and costained with rabbit MeCP2 pAb, dilution 1:5,000 (Red). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. Calbindin D-28K, often used as a Purkinje cell marker, is prominently detected in dendrites and perikarya of these cells in the cerebellar molecular layer. The MeCP2 antibody selectively stains nuclei of neuronal cells.

Amb optogenetic stimulation in Calb1cre or Ghsrcre mice results in apnea mediated by non-cholinergic neurons.Data are from same stimulation trials as Fig. 4. e) Immunostaining of ACP neurons in Amb in a Calb1cre mouse injected with AAV-DIO-bReaChES-eYFP in Amb where injection failed to target ACP neurons (red), but still targeted nearby interneurons and fibers (green). Note lack of eYFP expression in ACP cell bodies (arrowheads). Bar, 25 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35650438), licensed under a CC-BY licence.

Phd2 deletion leads to activation of the Akt–mTOR pathway. a RPPA analysis of tumors with homozygous deletion of Phd2. Tumor tissues from Tyr::CreER; BRafV600E; Phd2−/− or Tyr::CreER; BRafV600E mice were processed and analyzed by RPPA assays. The analyses identified proteins that were significantly changed in mouse melanomas compared to nevi. b Activation of Akt–mTOR pathway after phd2 deletion. Tumor tissues were processed and western blots showed stabilization of HIF-1 alpha and HIF-2 alpha proteins after Phd2 depletion. Increased phosphorylation of Akt, 4EBP1 and S6K was observed in tumors from Tyr::CreER; BRafV600E; Phd2−/− compared with those of Tyr::CreER; BRafV600E mice. c Re-expression of Phd2 inhibits the Akt–mTOR pathway. A BRafV600E; Phd2−/− mouse melanoma cell line was established from melanomas in Tyr::CreER; BRafCA; Phd2lox/lox mice. Phd2 was ectopically reintroduced in these tumor cells. Western blot analysis showed that degradation of HIF-1 alpha and HIF-2 alpha proteins with decreased expression of VEGFR2 decreased phosphorylation of Akt, 4EBP1 and S6K. d Pharmacological inhibition (FM19G11) of HIF pathway in BRafV600E; Phd2−/− melanoma cells. A similar but more pronounced inhibition of the Akt–mTOR pathway was observed using the HIF inhibitor. beta -Actin was used as a loading control. Results are representative of three independent experiments Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30575721), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

UDM1 is required for RNF168-mediated 53BP1 recruitment.a Schematic illustration of RNF8 and RNF168 structural domain organization, RNF168 fragments, and chimeric proteins used as in b and c. FHA–forkhead-association domain; coiled-coil–coiled-coil domain; RING–ubiquitin E3 ligase RING domain; Rs-arginine anchor; LRM–LR motif; UMI–UIM-and MIU-related ubiquitin binding domain; MIU–motif interacting with ubiquitin. b–c Transient transfection of GFP-tagged RNF168 fragments and chimeric proteins in U2OS RNF168 KO cells. Cells were irradiated with 10 Gy and allowed to recover for 1 h followed by immunofluorescence analysis using 53BP1 antibody as indicated. Cells were counterstained with DAPI. Repeated at least two times independently with similar results. Source data are provided as Source Data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32424115), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

, , ,

Applications: , , ,

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB300-217

NF-H Antibody

(2 Reviews)

(7 Publications)

0.05 ml

NB300-217

Western Blot: NF-H Antibody [NB300-217] - Analysis of spinal cord lysates from different species using chicken pAb to NF-H, NB300-217, dilution 1:20,000 in green: [1] protein standard (red), [2] rat, [3] mouse, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the phosphorylated from of NF-H. The protein from different species is known to have different SDS-PAGE molecular weights, with large species generally expressing larger proteins. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with this antibody. The antibody does not recognize non-phosphorylated forms of NF-H.

Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Imaging of Feline optic nerve at antibody dilution 1:5000. This image was submitted via customer Review.

Immunohistochemistry Free-Floating: NF-H Antibody [NB300-217] - Analysis of a rat cerebellum section stained with NF-H antibody, dilution 1:5,000 (Red), and costained with rabbit GFAP pAb, dilution 1:5,000 Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free floating sections were stained with above antibodies. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes and other glial cells.

Western Blot: NF-H Antibody [NB300-217] - Analysis of 200kDa Neurofilament Heavy expression in rat spinal cord extract. The first lane is Coomassie Brilliant Blue stained and the second lane is probed with chicken anti-Neurofilament Heavy antibody NB300-217.

Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Rat neurons stained with 200kDa Neurofilament Heavy Antibody NB300-217.

Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - SH-SY5Y cells stained with 200kDa Neurofilament Heavy Antibody NB300-217 (red) and Fibrillarin Antibody NB300-269 (green). Nuclear DNA is stained with Hoechst dye (blue).

Immunocytochemistry/Immunofluorescence: NF-H Antibody [NB300-217] - Mixed rat neuron/glial cultures stained with Neurofilament Light (NF-L) antibody NBP1-05217 [green] and Neurofilament Heavy (NF-H) antibody NB300-217 [red]. Blue is a DNA stain. NB300-217 binds primarily to the phosphorylated axonal forms of NF-H, in contrast to NBP1-05217 which stains both axonal and dendritic/perikaryal neurofilaments. The surrounding axonal profiles are orange due to staining of both the NF-H and NF-L antibodies.

Immunohistochemistry-Frozen: NF-H Antibody [NB300-217] - Canine optic nerve, antibody specifically labelled the axons (Green). Blue is DAPI. Image taken with an epifluorescent microscope and was incubated at 1:10000 for 1hr at RT.. Image from verified customer review.

Immunohistochemistry-Paraffin: NF-H Antibody [NB300-217] - NF-H immunoreactivity in a FFPE section of mouse trigeminal ganglion. Antibody was diluted 1 to 2000 and left on sections for 1h at room temperature. Image from verified customer review.

Immunohistochemistry-Frozen: Chicken Polyclonal NF-H Antibody [NB300-217] - Fixed frozen section of mouse optic nerve showing neurofilament heavy NB300-217 in green and S100B in red. NB300-217 was diluted 1 in 4000 and was left on tissue sections overnight at room temperature. Primary was detected with donkey anti chicken conjugated to Alexa 488. Image from a verified customer review.

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of canine cecum showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of feline colon showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of equine jejunum showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.

Immunohistochemistry-Paraffin: Chicken Polyclonal NF-H Antibody [NB300-217] - FFPE section of human colon showing NF-H NB300-217 immunoreactivity in enteric neurons. Primary antibody was diluted 1 in 2000 and left on tissue sections for 30m at room temperature. Secondary was donkey anti chicken HRP. Image from a verified customer review.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe

Applications:

, , , ,

Applications: , , , ,

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Conjugate:

Unconjugated

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NB300-222

NF-M Antibody

(1 Review)

(11 Publications)

0.25 ml

NB300-222

Western Blot: NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.

Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-222] - 160kDa Neurofilament Medium Antibody [NB300-222] - View of mixed neuron/glial cultures stained with NB300-222 (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.

Immunohistochemistry Free-Floating: NF-M Antibody [NB300-222] - Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M, NB300-222, dilution 1:1,000 in red, and costained with mouse mAb to CNPase, dilution 1:500, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. The NF-M antibody labels the axons of basket cells and other neurons, while the CNP antibody stains oligodendrocytes, cells that form the myelin sheathes around axons.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch, Dr, Eq

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Dr, Eq

Applications:

, , , , ,

Applications: , , , , ,

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Conjugate:

Unconjugated

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NBP1-05194

alpha-Synuclein Antibody (2A7)

(9 Publications)

0.1 ml

NBP1-05194

Immunohistochemistry Free-Floating: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Analysis of rat cerebellum section costained with mouse mAb to alpha-synuclein NBP1-05194, dilution 1:1,000, in red, and rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in synaptic regions, while the GFAP antibody stains the filamentous cytoskeleton of Bergmann glia and astrocytic cells.

Simple Western: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Synuclein-alpha Antibody (2A7) [NBP1-05194] - Simple Western lane view shows a specific band for alpha-Synuclein in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: alpha-Synuclein Antibody (2A7) [NBP1-05194] - Western blot analysis of tissue lysates using mAb to alpha-synuclein NBP1-05194. dilution 1:1,000 in green. Lane [1] protein standard in red, [2] whole rat brain lysate, [3] rat spinal cord lysate. Strong band at about 15kDa corresponds to alpha-synuclein protein.

Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody (2A7) [NBP1-05194] - Mixed neuron-glial cultures stained with NBP1-05194, our monoclonal antibody to alpha-synuclein (red) and chicken polylclonal antibody to MAP2 NB300-213 (green). The alpha-synuclein antibody stains vesicular structures the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-synuclein than others. The blue channel shows the localization of DNA.

Flow Cytometry: alpha-Synuclein Antibody (2A7) [NBP1-05194] - An intracellular stain was performed on SK-MEL-28 cells with alpha-Synuclein [2A7] Antibody NBP1-05194AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Reactivity:

Hu, Mu, Rt, Po, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-05194AF350
Alexa Fluor 405	NBP1-05194AF405
Alexa Fluor 488	NBP1-05194AF488
Alexa Fluor 532	NBP1-05194AF532
Alexa Fluor 647	NBP1-05194AF647
Alexa Fluor 700	NBP1-05194AF700
Alexa Fluor 750	NBP1-05194AF750
Allophycocyanin	NBP1-05194APC
Biotin	NBP1-05194B
DyLight 350	NBP1-05194UV
DyLight 405	NBP1-05194V
DyLight 488	NBP1-05194G
DyLight 550	NBP1-05194R
DyLight 594	NBP1-05194DL594
DyLight 650	NBP1-05194C
DyLight 680	NBP1-05194FR
DyLight 755	NBP1-05194IR
FITC	NBP1-05194F
HRP	NBP1-05194H
Janelia Fluor 549	NBP1-05194JF549
Janelia Fluor 646	NBP1-05194JF646
PE	NBP1-05194PE
mFluor Violet 450 SE	NBP1-05194MFV450
mFluor Violet 500 SE	NBP1-05194MFV500
mFluor Violet 610 SE	NBP1-05194MFV610

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NBP2-25156

MAP2 Antibody (4H5)

(4 Reviews)

(3 Publications)

0.1 ml

NBP2-25156

Immunocytochemistry/Immunofluorescence: MAP2 Antibody (4H5) [NBP2-25156] - Mixed neuron and glia cultures stained with NBP2-25156 (green), and NB300-135 rabbit antibody to NF-H (red) and DNA (blue). NBP2-25156 reveals strong cytoplasmic staining for of dendrites and perikarya, which does not overlap with the NF-H antibody, which primarily binds to axons.

Immunohistochemistry Free-Floating: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of a rat hippocampus section stained with mouse mAb to MAP2, NBP2-25156, dilution 1:2,000 in green, and costained with rabbit pAb to FOX3/NeuN, dilution 1:2,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The NBP2-25156 antibody labels MAP2 protein in the perikarya and dendrites of most neurons while the FOX3/NeuN antibody selectively stains nuclei and proximal soma of neuronal cells.

Western Blot: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of tissue and cell lysates using mouse mAb to MAP2, NBP2-25156, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, and [4] embryonic rat cortical neuron-glial cell lysate. A band at about 280 kDa corresponds to the MAP2A and MAP2B proteins.

Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Analysis of MAP2 in a 1% PFA fixed mouse brain section using anti-MAP2 (red) and NeuN (green) antibodies. Image from verified customer review.

Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Staining in mouse brain. Image from a verified customer review.

Immunohistochemistry-Paraffin: MAP2 Antibody (4H5) [NBP2-25156] - Staining of a 1% PFA fixed mouse cortical region section with anti-MAP 2 (dilution 1:300; Alexa 568 red). Image from a verified customer review.

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-25156AF350
Alexa Fluor 405	NBP2-25156AF405
Alexa Fluor 488	NBP2-25156AF488
Alexa Fluor 594	NBP2-25156AF594
Alexa Fluor 647	NBP2-25156AF647
Alexa Fluor 700	NBP2-25156AF700
Alexa Fluor 750	NBP2-25156AF750
Biotin	NBP2-25156B
DyLight 350	NBP2-25156UV
DyLight 405	NBP2-25156V
DyLight 488	NBP2-25156G
DyLight 550	NBP2-25156R
DyLight 594	NBP2-25156DL594
DyLight 650	NBP2-25156C
DyLight 680	NBP2-25156FR
DyLight 755	NBP2-25156IR
FITC	NBP2-25156F
HRP	NBP2-25156H
Janelia Fluor 549	NBP2-25156JF549
Janelia Fluor 646	NBP2-25156JF646
mFluor Violet 450 SE	NBP2-25156MFV450
mFluor Violet 500 SE	NBP2-25156MFV500
mFluor Violet 610 SE	NBP2-25156MFV610

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NBP1-92695

TDP-43/TARDBP Antibody (3H8)

(6 Publications)

0.1 ml

NBP1-92695

Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of whole brain lysates and nuclear inreached extract from whole brain using mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. Strong band at 43 kDa mark corresponds to TDP43 protein.

Immunocytochemistry/Immunofluorescence: TARDBP Antibody (3H8) [NBP1-92695] - NBP1-92695 was used to stain a section of formalin fixed adult rat brain, specifically the hippocampus. Hippocampal neuron nuclei are stained strongly. Chicken antibody to GFAP (NBP1-05198, green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue). The TARDBP (3H8) antibody stains neuronal nuclei strongly and the nuclei of some non-neuronal cells much more weakly. Neuronal nuclei therefore look crimson, since they are both red due to the content of TARDBP and blue due to their content of DNA, stained blue with DAPI.

Immunohistochemistry Free-Floating: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of rat hippocampus section stained with mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in red, and costained with chicken pAb to GFAP,, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The TDP43 protein is concentrated in the nuclei of hippocampal neurons, while the GFAP antibody stains the intermediate filament network of astroglial cells.

<b>Genetic Strategies Validation. </b>Knockout Validated: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Lysates of HAP1 (WT) and TDP-43/TARDBP (KO) were prepared, and 50 ug of protein was processed for western blot with the TDP-43/TARDBP antibody. The Ponceau stained transfers of the blot is shown (right). Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).

Immunoprecipitation: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 ug of the TDP-43/TARDBP antibody pre-coupled to Dynabeads protein G. Sample was washed and processed for immunoblot with the TDP-43/TARDBP antibody. The Ponceau stained transfer of the blot is shown. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).

Knockout Validated: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 (WT) and TDP-43/TARDBP (KO) cells were labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with glass bottom. Cells were stained with the TDP-43/TARDBP antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined on both channels with green and magenta dashed line, respectively. Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).

Reactivity:

Hu, Mu, Rt, Po, Bv, Eq

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Eq

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-92695AF350
Alexa Fluor 405	NBP1-92695AF405
Alexa Fluor 488	NBP1-92695AF488
Alexa Fluor 532	NBP1-92695AF532
Alexa Fluor 594	NBP1-92695AF594
Alexa Fluor 647	NBP1-92695AF647
Alexa Fluor 700	NBP1-92695AF700
Alexa Fluor 750	NBP1-92695AF750
Biotin	NBP1-92695B
DyLight 350	NBP1-92695UV
DyLight 405	NBP1-92695V
DyLight 488	NBP1-92695G
DyLight 550	NBP1-92695R
DyLight 594	NBP1-92695DL594
DyLight 650	NBP1-92695C
DyLight 680	NBP1-92695FR
DyLight 755	NBP1-92695IR
FITC	NBP1-92695F
HRP	NBP1-92695H
Janelia Fluor 549	NBP1-92695JF549
Janelia Fluor 646	NBP1-92695JF646
mFluor Violet 450 SE	NBP1-92695MFV450
mFluor Violet 500 SE	NBP1-92695MFV500
mFluor Violet 610 SE	NBP1-92695MFV610

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